Ratonga Whakawhanake Paturopi Monoclonal

Alpha Lifetech Inc.ka taea te whakarato i te matapae epitope, te hanga peptide, te whakaaturanga pūmua, te hanga paturopi polyclonal, monoclonal rānei, me te purenga paturopi mai i te ahurea pūtau, te wai ascites, te serum katoa rānei.

Alpha Lifetech Inc.He mātau ki te whakarato i ngā ratonga pūtau pumau ki ngā kiritaki tae atu ki ngā pūtau hybridoma, ngā pūtau whakatika ira, ngā pūtau knockdown, me ngā pūtau overexpression. E ngana ana mātou ki te tuku ratonga pūtau ritenga matatau ki ia kiritaki, ā, kua whakamātauhia, kua pono hoki ā mātou ratonga.

Rautaki Whakawhanake Paturopi

1. Te hoahoa me te whakarite i te paturopi
Ka taea e ēnei antigens te hanga pūmua, peptides, me ētahi atu ngota rānei. Ko te hoahoa i ngā immunogens me whai huarahi whānui e whakauru ana i te mōhiotanga ki te hanganga antigen, te immunology, me ngā pūnaha tuku. Kei a Alpha Lifetech te hangarau hoahoa antigen ka taea te hoahoa i ngā immunogens mō ngā kiritaki e hiahia ana.
Ka whakaratohia e Alpha Lifetech ngā hua pūmua hangarua hei whiriwhiri mā ngā kiritaki. He maha ā mātou pūnaha whakaputa pūmua, pērā i te pūnaha whakaputa E. coli, te pūnaha whakaputa rewena, te pūnaha whakaputa pepeke-baculovirus, te pūnaha whakaputa kararehe, me ētahi atu, hei whakaputa i ngā pūmua hangarua e hiahiatia ana e koe mō te rangahau.

2. Te ārai mate kararehe
Ka taea e Alpha Lifetech te whakarato i ngā ārai mate ki ngā kiritaki, ā, kei te wātea hoki ngā kararehe ārai mate pēnei i te kiore, te rāpeti, te hipi, te ārapa, te kiore kini, te hāmite, me ētahi atu hei whiriwhiri māu.

3. Ngā tikanga whakaputa paturopi monoclonal

●Te Hanga Mabs mā te hangarau Hybridoma
Ko te Hybridoma he pūtau ranu i hangaia mā te whakakotahitanga o ngā momo pūtau e rua, arā, ko te raina pūtau myeloma kiore me ngā pūtau plasma (lymphocyte B), ka taea e te pūtau heterozygous te whakaputa paturopi tonu ki te antigen e hiahiatia ana i roto i te vitro. Ko te whakaputanga o te raina pūtau hybridoma e toru ngā wāhanga: te hoahoa antigen (haptens, ngota iti, me ngā peptides), te werohanga kararehe, te whakakotahitanga pūtau, me te tirotiro clone pai. Mā te whakamahi i ngā tikanga werohanga ahurei, ka whiwhi mātou i ngā whai huatanga whakakotahitanga pūtau tino teitei (1-10 hybridomas mō ia mano pūtau B) me ngā titers paturopi teitei, ka whakanui ake i tō mātou reiti angitu i roto i ngā whakamātautau tātaritanga e whai ake nei tae atu ki te tirotiro matū koiora, ngā whakamātautau pūtau-in-vitro, me ngā rangahau kararehe.

●Te Hanga Mabs mā te hangarau whakaatu phage
Mā te whakaaturanga phage ka tukuna he tikanga anō mō ​​te whakaputa i ngā paturopi monoclonal. Ka wehea ngā pūtau toto-B mai i te toto kararehe, ā, ka tangohia ō rātou mRNA. Mā te PCR, ka hurihia tēnei mRNA hei cDNA, e whakanui ana i ngā wāhanga VH me VL katoa. Kātahi ka tāruatia ēnei wāhanga ki roto i tētahi vector, ko te tikanga he wāhanga taurangi mekameka kotahi (scFv), me te pūmua PIII o tētahi bacteriophage. Muri iho, ka whakamahia tēnei hanganga hei pangia te E. coli, ka hua ake he whare pukapuka kei roto tata ki te 10^10 ngā pūtau mā te werohanga ki tētahi phage āwhina. Kātahi ka taea e te E. coli te huna i te bacteriophage e pupuri ana i ngā wāhanga VH me VL hei wāhanga o tōna uhi. Whai muri i tēnei, ka taea te whiriwhiri i ngā wāhanga VH me VL motuhake e aro ana ki te antigen e hiahiatia ana, ā, ka whakamahia hei whakahoki anō i te E. coli ki te bacteriophage. Kātahi ka wehea ngā pūtau kei roto te plasmid, ā, ka raupapahia.

4. Te tirotiro honohono

●Te Hononga Pūtau me te Kōwhiringa
Kātahi ka honoa ngā pūtau-B kua hauhaketia ki ngā pūtau myeloma, arā, ngā pūtau matepukupuku ka taea te tipu haere tonu i roto i te ahurea. Ko te tikanga o te whakakotahitanga ka tutuki mā te whakamahi i tētahi tikanga pēnei i te whakakotahitanga polyethylene glycol (PEG).
I muri i te hanumitanga, ka whakatōkia ngā pūtau hybridoma ki roto i tētahi reo whiriwhiri e āhei ai ngā hybridoma anake te ora. Kei roto i te reo te aminopterin, e ārai ana i te tipu o ngā pūtau myeloma kāore i hanumi.

●Te Matawai me te Whakakōpiri
Ka tirotirohia ngā pūtau hybridoma e puta mai ana mō te whakaputanga o ngā paturopi e hāngai ana ki te antigen ūnga. Ko te tikanga ka mahia tēnei mā te whakamahi i ngā tikanga pēnei i te enzyme-linked immunosorbent assay (ELISA), te Western blot, te immunoprecipitation, me te flow cytometry.

Kia kitea ngā pūtau hybridoma e whakaputa ana i te paturopi, ka tāruatia rātou hei whakaputa i tētahi taupori o ngā pūtau ōrite. Mā tēnei ka whakarite kia ōrite te paturopi e hangaia ana e ia pūtau hybridoma.

5. Manatoko Mahi a te Paturopi
Ka whakarato a Alpha Lifetech i te whakamana mahi a te paturopi, pērā i te Western blotting, te immunohistochemistry, ngā whakamātautau mahi rānei, hei tautuhi i ngā paturopi monoclonal me te whakaū i te motuhaketanga, te hononga, me te mahi a te paturopi.

6. Te arotautanga o te paturopi
Te purenga o ngā paturopi mai i te waikura ahurea mā te whakamahi i ngā tikanga pēnei i te chromatography hononga pūmua A, pūmua G rānei, ā, ka taea hoki e Alpha Lifetech te whakarato i ngā tikanga whakarerekētanga paturopi hei whakapai ake i te hononga paturopi.

7. Te hanga paturopi
Ka taea e Alpha Lifetech te aromatawai i ngā paturopi kaitono mō ngā tono tātari tere-kaha me te whakaputanga nui.