Pūhara Whakawhanake Paturopi Whakaaturanga Phage

Me ōna wheako kaupapa whānui ki te kimi paturopi, ka taea e Alpha Lifetech te whakarato ratonga paturopi monoclonal me polyclonal kua whakaritea mō ngā momo maha, tae atu ki ngā ratonga hanga whare pukapuka paturopi me te tirotiro. Ka taea e Alpha Lifetech te whakarato ki ngā kiritaki he hua paturopi me te paturopi recombinant kua whakapūmautia te kounga, me ngā ratonga e rite ana, hei whakarite i ngā paturopi whai hua, tino motuhake, me te pumau. Mā te whakamahi i ngā papaaho paturopi whānui me te hangarau paturopi, ka whakaratohia e mātou ngā ratonga e kapi ana i te taha whakarunga me te taha whakararo o te hanga paturopi, tae atu ki ngā ratonga hangarau pēnei i te whakarite paturopi, te purenga, te raupapatanga paturopi, me te whakamana.

He tūāpapa kitenga paturopi pakari tā Alpha Lifetech e whakarato ana i ngā ratonga maha ki ngā kiritaki tae atu ki te ārai mate kararehe, te hanga me te tirotiro i te whare pukapuka paturopi whakaatu phage, te raupapatanga paturopi, te whakaaturanga paturopi, te purenga paturopi, te whakamana paturopi, me te tapanga paturopi i runga i te hangarau hybridoma, te hangarau pūtau B kotahi, te hangarau whakaatu phage, me te maha atu. He raina whakaputa paerewa tā Alpha Lifetech, ā, hei tāpiri atu ki ngā ratonga whakarite paturopi, ka taea hoki te whakarato i ngā ratonga tautoko pēnei i te whakatangatatanga paturopi, te pakeketanga o te hononga paturopi, te hoahoa me te whakawhanaketanga rongoā ADC, me te hoahoa raupapatanga whai muri a CAR-T. I taua wā anō, kua hangaia e Alpha Lifetech he whare pukapuka paturopi i runga i ngā tūāpapa hangarau whakaatu phage M13, T4, me te T7, me te kaha rokiroki tae atu ki te 10^8-10^9. Ko te reiti pai, te reiti whakauru, me te kanorau o te whare pukapuka ka eke katoa ki te neke atu i te 90%.

Hangarau Whakaaturanga Phage

I te tuatahi, i whakaritea te hanga paturopi monoclonal mā te whakamahi i te hangarau paturopi monoclonal hybridoma hei whakaputa i te paturopi monoclonal hybridoma kiore. Ina koa, mā te whakakotahi i ngā pūtau whēkau mai i ngā kiore kua werohia ki ngā pūtau myeloma mai i te tangata, i ngā kiore rānei, ka hangaia ngā pūtau hybridoma, e whakaputa ana i ngā paturopi paturopi monoclonal hybridoma motuhake. Mā te whakarerekētanga tangata o ngā paturopi monoclonal kiore me te whakarerekētanga ira o ngā paturopi hei hoatu ki a rātou he rohe immunoglobulin tangata pumau, ka taea te whakaiti i tō rātou ārai mate. He whānuitia te whakamahinga o te hangarau paturopi monoclonal i roto i ngā tono hauora me te haumanu.

Ka taea e Alpha Lifetech te whakarato ratonga hangarau paturopi kotahi-mutunga mai i te hoahoa kawa rangahau haumanu-mua ki te kimi i ngā hononga rongoā paturopi (ADC) me te whakamana kararehe. Ka taea e ngā hononga rongoā paturopi (ADC) te tuku rongoā chemotherapy ki ngā pūtau matepukupuku. Whai muri i te herea ki ngā whāinga motuhake kua whakaatuhia ki ngā pūtau matepukupuku, ka tukuna e te ADC ngā rongoā cytotoxic ki ngā pūtau matepukupuku. Ka taea hoki e Alpha Lifetech te whakarato ki ngā kiritaki he ratonga pakari hononga paturopi whānui. Mā te arotau ira whakawhiti matatau me te hangarau tirotiro whakaaturanga phage teitei-whakaputa, ka tirotirohia tuatahitia ngā paturopi me te kaha motuhake, kātahi ka whakaurua ngā ira whakawhiti waikawa amino hei whakaputa i ngā momo rerekētanga. Muri iho, ka aromatawaihia, ka tirotirohia hoki ngā paturopi kaha teitei mā te whakamahi i ngā tikanga tirotiro. Whai muri i ngā rauna maha o te arotau me te tātari hanganga, ka whiwhihia ngā paturopi he kaha ake te kaha me te motuhaketanga kaha ake.

Ka taea e Alpha Lifetech te hanga i ngā momo whare pukapuka whakaatu paturopi phage, tae atu ki ngā whare pukapuka ārai mate, ngā whare pukapuka taketake, ngā whare pukapuka hanga-ā-rua, me ngā whare pukapuka hanga-ā-rua. I runga i te kaha nui o ngā whare pukapuka paturopi, ka taea te whiwhi paturopi tino motuhake. Ka taea te whakarato i ngā momo kawe phagemid maha pērā i te pMECS, pComb3X, me te pCANTAB 5E, me ngā momo momo pērā i te TG1 E. coli, XL1 Blue, me te ER2738. Haunga te kaha o te whare pukapuka tae atu ki te 10^9, he teitei hoki te tere whakauru maramara ūnga o te whare pukapuka, e hanga ana i ngā āhuatanga e ranea ana mō te tirotiro i ngā paturopi e makona ai ngā kiritaki. Kei te whakaatuhia te tūtohi rere o te ratonga whakarite paturopi a Alpha Lifetech i runga i te hangarau whakaatu phage i te Pikitia 1.

Pikitia 1. Kauwhata tukanga mō te whakarite i ngā paturopi monoclonal i runga i te hangarau whakaatu phage.

Rerenga Mahi Whakaputa Paturopi Whakaaturanga Phage

Ngā Hipanga	Ihirangi Ratonga	Rārangi Wā
Hipanga 1: Te werohanga ārai mate mō ngā kararehe	(1) 4 ngā werohanga ārai mate mō ngā kararehe, 1 te horopeta ārai mate tāpiri, e 5 katoa ngā werohanga i werohangatia. (2) I mua i te kohi i te werohanga, i puta te hua kino o te toto, ā, i mahia te ELISA i te tuawhā o ngā horopeta hei kite i te taumata o te toto. (3) Mena ka tutuki i te taumata o te paturopi toto o te tuawhā o ngā horopeta ngā whakaritenga, ka tukuna tētahi atu horopeta ārai mate i ngā rā e 7 i mua i te kohikohinga toto. Mena kāore e tutuki i ngā whakaritenga, ka haere tonu te ārai mate noa. (4) Te kaha kua tohua, te kohikohi toto me te wehenga o ngā monocyte	10 wiki
Hipanga 2: Te Whakaritenga cDNA	(1) Tangohanga RNA Katoa PBMC (Kete Tangohanga RNA) (2) Te whakarite RT-PCR pono o te cDNA (kete whakawhiti whakamuri)	1 rā
Hipanga 3: Te Hanganga o te Whare Pukapuka Paturopi	(1) Mā te whakamahi i te cDNA hei tauira, i whakanuia ngā ira mā te whakamahi i ngā huringa e rua o te PCR. (2) Te hanga me te whakawhiti ira: te honohono ira o te phagemid vector, te whakawhiti electroporation o ngā huakita manaaki TG1, te hanga o te whare pukapuka paturopi. (3) Tāutu: Tīpakohia matapōkeretia kia 24 ngā ira tārite, he pai te tere o te tautuhi PCR + he tere whakauru. (4) Te āwhina i te whakarite i te phage: Te whakanui i te phage M13+te purenga. (5) Te whakaoranga o te whare pukapuka whakaaturanga Phage	3-4 wiki
Hipanga 4: Tirotiro Whare Pukapuka Paturopi (3 rauna)	(1) Tātaritanga 3-wāhanga taunoa (tātaritanga wāhanga-totoka): tātaritanga pēhanga hei tango i ngā paturopi kore-motuhake ki te taumata teitei rawa atu. (2) Te whakanui i te bacteriophage mā te kōwae kotahi kua tīpakohia + te whakaaturanga IPTG i whakaohohia + te kitenga ELISA o ngā bacteriophage pai. (3) I tīpakohia ngā ira katoa mō te raupapatanga ira.	4-5 wiki

Painga o te Hangarau Whakaaturanga phage

He nui ngā wheako o Alpha Lifetech i roto i te ao whakawhanake paturopi. I roto i ngā tau, kua whakatūria e Alpha Lifetech tētahi tūāpapa whakawhanake paturopi whānui.

Ratonga Tautoko

Ka taea e mātou te whakarato i ngā ratonga hanga whare pukapuka ārai mate kararehe me ngā ratonga tirotiro whare pukapuka paturopi taiao e ai ki ngā hiahia o te kiritaki.

Whāinga Maha

He maha ngā ratonga kimi paturopi ūnga e wātea ana: ngā pūmua, ngā peptide, ngā ngota iti, ngā huaketo, ngā pūmua kiriuhi, te mRNA, me ētahi atu.

Ngā Wētera Maha

Ratonga hanga whare pukapuka whaiaro, ka taea e mātou te whakarato i ngā momo kawe bacteriophage tae atu ki te PMECS, pComb3X, me te pCANTAB 5E, me te whakarerekē i a rātou kia rite ki ngā hiahia o te kiritaki.

Pūhara Pakeke

Ka taea e te kaha rokiroki te eke ki te 10 ^ 8-10 ^ 9, ko ngā reiti whakaurunga katoa kei runga ake i te 90%, ā, ko te kaha o ngā paturopi i whiwhihia mā te tirotiro kei te taumata nM pM.

RATONGA E Pā Ana

Ngā Rautaki Whakawhanaketanga Paturopi Maha

Ratonga Whakawhanake Paturopi Monoclonal

Pūhara Hangarau Hybridoma

Pūhara Tātari Pūtau B Kotahi

Pūhara Whakawhanake Paturopi Whakaaturanga Phage

te whanaketanga o te paturopi monoclonal-Alpha Lifetech

Ratonga Whakawhanake Paturopi Monoclonal

Ka taea e mātou te whakarato ratonga whakawhanake paturopi monoclonal kounga teitei, tino parakore, me te tino motuhake, tae atu ki te hanga paturopi monoclonal kiore me ngā paturopi monoclonal rāpeti.

Pūhara Hangarau Hybridoma

Tae atu ki te kaupapa werohanga ārai mate, ngā ratonga whakarite paturopi, te purenga paturopi, te raupapatanga tere o te paturopi, te whakamana paturopi, me ētahi atu

Pūhara Tātari Pūtau B Kotahi

He painga tō Alpha Lifetech mō te wā tirotiro me te whiwhi paturopi kounga teitei. Ka taea e ia te whakarato i te hoahoa paturopi, te hanga, me te whakarerekētanga, te ārai mate kararehe, te tirotiro whakarei ake i te pūtau B kotahi, te raupapatanga pūtau kotahi.