He Kupu Whakataki ki ngā Whare Pukapuka Paturopi Kua Hangaia

Ko te whare pukapuka paturopi i hangaia, e mōhiotia ana ko te whare pukapuka De novo, he tikanga hangai e whakamahi ana i ngā tikanga pēnei i te hanganga DNA, te whakaaturanga phage rānei hei hoahoa me te hanga i ngā rohe taurangi paturopi katoa, tae atu ki ngā rohe anga me ngā CDR, me te kore e whakawhirinaki ki ngā whare pukapuka paturopi hou.

Ka hangaia te whare pukapuka paturopi haurua-hanga mā te whakakotahi i ngā whare pukapuka paturopi tūturu me te kanorau hanga. Ko te tikanga, ko te hanga i ngā oligonucleotides DNA hei whakaputa i tētahi huinga o ngā rohe whakatau tautoko rerekē (CDR), kātahi ka honoa ki tētahi anga paturopi pumau i ahu mai i ngā pūtake tūturu pērā i ngā pūtau B tangata, kararehe rānei. Ka whakaurua e te CDR hanga te kanorau ki roto i te whare pukapuka, ka hua ake he paturopi e aro ana ki te whānuitanga o ngā epitope. Ka whakaratohia e te whare pukapuka paturopi haurua-hanga he taurite i waenga i te kanorau tūturu o te pūnaha ārai mate me te kanorau whakahaeretia e tutukihia ana mā ngā tikanga hanga.

Ko te rerekētanga i waenga i ngā whare pukapuka paturopi hangai me ngā whare pukapuka paturopi hangai e whakawhirinaki ana ki te pūtake o ngā ira immunoglobulin. I taua wā, ko te rerekētanga i waenga i te whare pukapuka paturopi haurua-hangai me te whare pukapuka paturopi hangai kei roto ko te mea tuatahi he wāhanga o ngā wāhanga paturopi hangai, pērā i te mekameka māmā, te mekameka taumaha rānei, ā, kotahi anake o aua mea ka hangaitia i roto i te vitro; ko te mea ia i hangaitia he mea ahu mai i te hanga horihori mā te PCR i roto i te vitro.

Ka taea e Alpha Lifetech te whakarato

Alpha Lifetech Inc.ka taea te tuku i tētahi whare pukapuka paturopi kua hangaia/kua hangaia mai i ngā kararehe me ngā tāngata i runga i tā mātou tūāpapa kimi paturopi ngaio. Me ngā tau o te wheako ki te whakarerekētanga ira me te hanga whare pukapuka, ka taea te hanga whare pukapuka kua hangaia ka taea te whakaputa paturopi hangai me te kaha me te motuhaketanga i tua atu i te kaha o ngā paturopi taiao.Alpha Lifetech'sE whakahīhī ana ngā tohunga ki te kī ka taea e mātou te kī taurangi he angitu teitei i roto i te hanganga o tētahi whare pukapuka paturopi kei roto ko ngā tārua motuhake 10^8 – 10^10.

Alpha Lifetech Inc.E whakahīhī ana mātou ki te tuku ratonga hanga whare pukapuka antibody whānui hei whakatutuki i ngā hiahia kanorau o ngā kairangahau o te ao, tae atu ki te scFv, Fab, VHH antibody, me ngā whare pukapuka kua whakaritea. Ko tā mātou whāinga he mārama me te whakatutuki i ngā hiahia o ngā kiritaki rerekē, me te āwhina i ngā raruraru e tū mai nei me ngā raruraru e puta ake ana i roto i ngā mahi rangahau.

Tukanga Ratonga Hanga Whare Pukapuka Paturopi Kua Hangaia

Hoahoa
Ko te wāhanga hoahoa ko te whiriwhiri i ngā anga paturopi me ngā rohe whakatau-whakakotahi (CDR) i runga i ngā raupapa paturopi o nāianei, i ngā raraunga hanganga rānei. Ka taea hoki te hoahoa i ngā paturopi CDR hangai hei whakauru i ngā mahi motuhake, hei whakarei ake rānei i ngā āhuatanga here.

Te Hanganga
Ka hangaia te DNA hangai e whakamārama ana i ngā raupapatanga paturopi kua hoahoatia, tae atu ki ngā rohe anga me ngā CDR, mā te whakamahi i ngā tikanga matū, i ngā tikanga whākōkī rānei.

Huihuinga
Ka huihuia ngā kongakonga DNA kua hangaia hei kawe whakaputanga paturopi mā te whakamahi i ngā tikanga pēnei i te PCR, te here, te huihuinga Gibson rānei. Kātahi ka taea te whakauru i ēnei kawe ki roto i ngā pūnaha whakaputanga pēnei i te kitakita, te rewena, ngā pūtau mamara rānei hei hanga paturopi.

Te Tirotiro me te Kōwhiringa
Ka tirotirohia, ka tīpakohia hoki ngā whare pukapuka paturopi kua hangaia mō ngā paturopi me ngā āhuatanga e hiahiatia ana mā te whakamahi i ngā tikanga tātari tere-whakaputa. Tērā pea ka uru atu ki tēnei ngā tikanga pēnei i te whakaaturanga phage, te whakaaturanga rewena, te whakaaturanga ribosome rānei, i runga i te whakatakotoranga me te tono o te whare pukapuka.

Ratonga Hanga Whare Pukapuka Paturopi Hangaia

Alpha Lifetech Inc.e whakarato ana i tētahi ratonga hoahoa kotahi-mutunga me te ratonga whakahiato o ngā whare pukapuka paturopi mā te here i ngā rārangi ira-V mekameka taumaha me te mekameka māmā ki roto i tētahi ira phage mā te whakamahi i te pūnaha whakakotahitanga motuhake-pae Cre-lox hei waihanga i tētahi rārangi o ngā Fab e whakaaturia ana i runga i te phage e 6.5 × 10^10 ngā tāruarua. I puta mai i te whare pukapuka ngā Abs ki ngā momo antigens maha, ko ētahi he hononga nanomolar. Ko te whakaputanga o te whare pukapuka paturopi tangata e hangai ana ki te hangarau whakaatu phage M13 (e whakaaturia ana i te pikitia i raro nei).