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Hybridoma and phage display each offer advantages in antibody discovery. Platform choice should depend on target type, workflow needs, timeline, and application—not reputation alone.

scFv linker design affects geometry, expression, and binding. Routine verification, starting with (G4S)3 and systematic testing, prevents artifacts like dimerization, aggregation, and false positives.

VHH purification differs from conventional antibodies due to small size and unique behaviors. Optimized Ni-NTA capture, SEC polishing, and aggregation control are key for consistent VHH production.

C5 is a terminal complement molecule cleaved into C5a and C5b, mediating inflammation and cell lysis. Dysregulated C5 causes PNH and aHUS. Anti-C5 antibodies and aptamers block C5 cleavage.

SELEX stringency must be actively managed across rounds. Controlled increases in washing and target pressure improve aptamer affinity, while planned stringency ramps deliver more consistent screening.

Antigen preparation impacts antibody discovery quality. Proper expression, purification, conjugation, and QC improve specificity, clone diversity, and reproducibility while reducing failedselections.

Focused peptide libraries use known motifs and smart design (alanine/positional scanning) to limit size while boosting hits. Alpha Lifetech streamlines design, synthesis, and screening.

Alpha Lifetech provides affinity detection services, including but not limited to SPR, BLI, ITC, MST, etc., to meet different experimental needs of customers and support them on their research journey

Alpha Lifetec provides multi-system protein expression and customized purification solutions, covering ervices from gene design, expression optimization, purification to functional validation.

KMD Bioscience can provide customized immortalization services for primary cells from different sources, delivering validated and stable cell lines.