Utumiki Wosanthula Makhalidwe a Aptamer

Alpha Lifetech yakhala ikupereka ntchito zokhudzana ndi aptamer kwa zaka zambiri, ndipo pakadali pano ikhoza kupereka njira zonse zopangira aptamer monga kuphatikiza aptamer, kuwunikira SELEX, kutsata kwapamwamba, komanso kukonza ndi kuwonetsa aptamer. Kutengera zabwino za njira ya SELEX yowunikira aptamers, Alpha Lifetech yawonjezera njira zambiri zowunikira aptamers.

Chiyambi cha Kusanthula kwa Makhalidwe a Aptamer

Kutsimikizira kugwirizana

Kugwirizana kwa aptamer komwe kwakonzedwa bwino kunatsimikiziridwa ndi njira zoyenera za kuyesa kogwirizanitsa ndi aptamer. Zitsanzo zikuphatikizapo Isothermal Titration Calorimetry (ITC), Flow Cytometry (FCM), ndi Surface Plasmon Resonance (SPR), Microfluidics, ndi zina zotero. Kugwirizana kwa aptamer nthawi zambiri kumawonetsedwa ndi dissociation constant (KD), kuchuluka kwa thupi komwe kumagwiritsidwa ntchito kuwerengera kuchuluka kwa kusagwirizana kwa mamolekyu mu reversible reaction. Mtengo wa KD ukakhala wocheperako, kumagwirizana kumakhala kokhazikika, ndiko kuti, kumagwirizana kumakhala kolimba; Mosiyana ndi zimenezi, mtengo wa KD ukakhala waukulu, kusagwirizana kumakhala kosasunthika komanso kufooka kwa kugwirizana kumakhala kofooka. Gawo ili ndilofunika kwambiri pozindikira momwe aptamer imagwirira ntchito, kuonetsetsa kuti attamer ikugwirizana bwino ndipo imatha kumangirira ku molekyulu yomwe ikufunidwa ndi kuyanjana kwakukulu komanso kusankha bwino pakugwiritsa ntchito.

Chithunzi 1 Njira yokhwima kwa aptamer affinity. Gwero lofotokozera:Kinghorn AB, Fraser LA, 2017.

Kutsimikizira ntchito

Kuwonjezera pa kutsimikizira kugwirizana, ntchito ya aptamer iyenera kutsimikiziridwa. Izi zikuphatikizapo kutsimikizira kukhazikika kwa aptamer, kulunjika kwake, komanso kuyanjana kwake ndi mamolekyu ena m'malo enaake. Zotsatira za kutsimikizira magwiridwe antchito zidzakhudza mwachindunji kufunika kwa aptamers mu kafukufuku wasayansi ndi ntchito zamafakitale.

Kutsimikizira Kwapadera

Kuyesera Kwampikisano: Kuthekera kwa aptamer kumangirira ku cholinga china chake kumayesedwa pamaso pa zolinga zina zofanana. Ngati aptamer ikhoza kumangirira mwachindunji ku molekyulu yomwe ikufunidwa popanda kusokonezedwa ndi mamolekyu ena, zimasonyeza kuti ili ndi luso lapadera.

Kuyesera kwa Cross reaction: Aptamer imalumikizidwa ku mndandanda wa zolinga zokhudzana kapena zosagwirizana kuti ione ngati ikugwirizana ndi cholinga china chake chokha, kuti itsimikizire kutsimikizika kwake.

Kutsimikizira kukhazikika

Kuyesa kuwonongeka kwa nyukiliya: Ma aptamer adakumana ndi kuchuluka kosiyanasiyana kwa ma nuclease ndipo kuwonongeka kwawo kudawonedwa. Poyerekeza kuchuluka kwa kuwonongeka nthawi zosiyanasiyana, mphamvu ya aptamer yolimbana ndi kuwonongeka kwa nyukiliya ikhoza kuyesedwa.

Kuyesa kukhazikika kwa kutentha ndi nthawi: Aptamer idayikidwa pansi pa kutentha ndi nthawi zosiyanasiyana kuti ione kukhazikika kwake ndi magwiridwe antchito ake. Izi zimathandiza kudziwa momwe angasungire ndi kugwiritsa ntchito bwino aptamer.

Kuzindikira zochita zamoyo

Njira yoyenera nthawi zambiri imasankhidwa malinga ndi cholinga chenicheni cha aptamer ya nucleic acid.

(1) Kuyesa mulingo wa mamolekyulu: Kugwiritsa ntchito njira za biology ya mamolekyulu, monga gel electrophoresis, Western blot, ndi zina zotero, kuti mudziwe zovuta zomwe zimapangidwa pambuyo poti aptamer iphatikizana ndi molekyulu yofunidwa, kapena kuzindikira kusintha kwa mulingo wa mafotokozedwe a molekyulu yofunidwa chifukwa cha aptamer.

(2) Kuyesa mulingo wa maselo: Pogwiritsa ntchito ukadaulo wokulitsa maselo, ma aptamer amaikidwa ndi maselo omwe akufuna kuti aone kusintha kwa zamoyo monga mawonekedwe a maselo, kuchulukana ndi apoptosis, ndikuwunika momwe ma aptamer amagwirira ntchito zamoyo.

(3) Kuyesa kwa mtundu wa nyama: Mu mitundu yoyenera ya nyama, ma aptamer a nucleic acid amaperekedwa mwa jakisoni kapena mankhwala, ndipo zizindikiro za thupi ndi kusintha kwa matenda a nyama zimawonedwa kuti ziwunikire ntchito yachilengedwe komanso chitetezo cha ma aptamer mu thupi.