Pulogalamu Yopangira Ma Antibody Yowonetsera Phage Display

Alpha Lifetech, yokhala ndi chidziwitso chachikulu pakupeza ma antibodies, ingapereke ntchito zodzitetezera za monoclonal ndi polyclonal kwa mitundu yosiyanasiyana ya ma antibodies, komanso ntchito zomanga ndi kufufuza ma antibodies ku laibulale ya ma antibodies. Alpha Lifetech ikhoza kupatsa makasitomala zinthu zodzitetezera za antibody ndi recombinant antibody zabwino kwambiri, komanso ntchito zofanana, kuti akonze ma antibodies ogwira mtima, apadera kwambiri, komanso okhazikika. Pogwiritsa ntchito nsanja zonse za ma antibodies ndi mainjiniya a ma antibodies, timapereka ntchito zokhudzana ndi kupanga ma antibodies kumtunda ndi kumunsi, kuphatikizapo ntchito zaukadaulo monga kukonzekera ma antibodies, kuyeretsa, kutsata ma antibodies, ndi kutsimikizira.

Alpha Lifetech ili ndi nsanja yopezera ma antibodies okhwima yomwe imapatsa makasitomala ntchito zosiyanasiyana kuphatikizapo chitetezo cha ziweto, kumanga ndi kuyesa laibulale ya ma antibodies a phage display, kutsata ma antibodies, kufotokoza ma antibodies, kuyeretsa ma antibodies, kutsimikizira ma antibodies, ndi kulemba ma antibodies kutengera ukadaulo wa hybridoma, ukadaulo wa selo limodzi la B, ukadaulo wowonetsa ma antibodies, ndi zina zambiri. Alpha Lifetech ili ndi mzere wokhazikika wopanga, ndipo kuwonjezera pa ntchito zokonzekera ma antibodies, ntchito zothandizira monga kupangitsa ma antibodies kukhala anthu, kukula kwa ma antibodies affinity, kapangidwe ndi chitukuko cha mankhwala a ADC, komanso kapangidwe ka CAR-T kotsatira kungaperekedwenso. Nthawi yomweyo, Alpha Lifetech yamanga laibulale ya ma antibodies kutengera nsanja zaukadaulo zowonetsera ma antibodies a M13, T4, ndi T7 phage, yokhala ndi mphamvu yosungira mpaka 10^8-10^9. Chiŵerengero chabwino, chiŵerengero cholowera, ndi kusiyanasiyana kwa laibulale zonse zitha kufika pa 90%.

Ukadaulo wowonetsa phage

Kupanga ma antibodies a monoclonal poyamba kunakonzedwa pogwiritsa ntchito ukadaulo wa hybridoma monoclonal antibody kuti apange ma antibodies a mbewa a hybridoma monoclonal. Makamaka, mwa kuphatikiza maselo a ndulu kuchokera ku mbewa zomwe zapatsidwa katemera ndi maselo a myeloma ochokera kwa anthu kapena mbewa, maselo a hybridoma amapangidwa, omwe amatulutsa ma antibodies enieni a hybridoma monoclonal antibody. Mwa kusintha kwa ma antibodies a monoclonal a mbewa ndi kusintha kwa majini a ma antibodies kuti awapatse dera lokhazikika la immunoglobulin ya anthu, mphamvu yawo ya chitetezo chamthupi imatha kuchepetsedwa. Kupanga ma antibodies a monoclonal kumagwiritsidwa ntchito kwambiri mu ntchito zamankhwala ndi zamankhwala.

Alpha Lifetech ikhoza kupereka ntchito zowunikira ma antibodies kuyambira pakupanga njira zofufuzira zamankhwala asanapezeke mpaka kupeza ma antibodies drug conjugates (ADCs) ndi kutsimikizira nyama. Ma antibodies conjugates (ADCs) amatha kupereka mankhwala a chemotherapy ku maselo a khansa. Pambuyo polumikizana ndi zolinga zinazake zomwe zimawonetsedwa pa maselo a khansa, ADC imatulutsa mankhwala a cytotoxic m'maselo a khansa. Alpha Lifetech ikhozanso kupatsa makasitomala ntchito zonse zokhudzana ndi ma antibodies affinity maturation. Ndi ukadaulo wapamwamba wokonza ma mutation ndi ukadaulo wowunikira ma phage, ma antibodies okhala ndi affinity inayake amayesedwa kaye, kenako ma amino acid mutations amayambitsidwa kuti apange mitundu yosiyanasiyana. Pambuyo pake, ma antibodies a affinity apamwamba adayesedwa ndikuyesedwa pogwiritsa ntchito njira zowunikira. Pambuyo pa kusanthula kambiri komanso kapangidwe kake, ma antibodies okhala ndi affinity yapamwamba komanso kutsimikizika kwamphamvu adapezeka.

Alpha Lifetech ikhoza kupanga mitundu yosiyanasiyana ya malaibulale owonetsa ma antibody phage, kuphatikizapo malaibulale oteteza chitetezo cha mthupi, malaibulale achilengedwe, malaibulale osapangidwa bwino, ndi malaibulale opangidwa. Kutengera mphamvu yayikulu ya malaibulale oteteza chitetezo cha mthupi, ma antibodies apadera kwambiri angapezeke. Ma vector ambiri a phagemid monga pMECS, pComb3X, ndi pCANTAB 5E angaperekedwe, komanso mitundu monga TG1 E. coli, XL1 Blue, ndi ER2738. Kuphatikiza pa mphamvu ya laibulale yofika 10^9, kuchuluka kwa zidutswa zomwe laibulale ikufuna kuyikamo ndikwapamwamba, zomwe zimapangitsa kuti pakhale mikhalidwe yokwanira yowunikira ma antibodies omwe amakhutiritsa makasitomala. Tchati cha ntchito yokonzekera ma antibody ya Alpha Lifetech yochokera paukadaulo wowonetsera ma phage ikuwonetsedwa mu Chithunzi 1.

Chithunzi 1 Chithunzi cha njira yokonzekera ma antibodies a monoclonal kutengera ukadaulo wowonetsera phage.

Kayendedwe ka Ntchito Yopanga Ma Antibody a Phage Display

Masitepe	Zamkati mwa Utumiki	Mndandanda wa Nthawi
Gawo 1: Katemera wa ziweto	(1) Katemera wa ziweto nthawi 4, katemera wowonjezera mlingo umodzi, mlingo wonse wa 5 wa katemera. (2) Seramu yoipa isanatengedwe katemera, ndipo ELISA idachitidwa pa mlingo wachinayi kuti ipeze titer ya seramu. (3) Ngati mlingo wa antibody wa seramu wa mlingo wachinayi ukukwaniritsa zofunikira, mlingo umodzi wowonjezera wa katemera udzaperekedwa masiku 7 magazi asanatengedwe. Ngati sukwaniritsa zofunikira, katemera wanthawi zonse udzapitirira. (4) Mphamvu yoyenerera, kusonkhanitsa magazi ndi kulekanitsa ma monocytes	Masabata 10
Gawo 2: Kukonzekera kwa cDNA	(1) PBMC Total RNA Extraction (RNA Extraction Kit) (2) Kukonzekera bwino kwambiri kwa cDNA (reverse transcription kit) kwa RT-PCR (reverse transcription kit)	Tsiku limodzi
Gawo 3: Kupanga Laibulale ya Antibody	(1) Pogwiritsa ntchito cDNA ngati chitsanzo, majini adakulitsidwa ndi ma PCR awiri. (2) Kapangidwe ndi kusintha kwa phage: gene splicing phagemid vector, kusintha kwa electroporation kwa mabakiteriya a TG1 host, kumanga laibulale ya antibody. (3) Kuzindikira: Sankhani mwachisawawa ma clones 24, PCR identification positive rate + insertion rate. (4) Kukonzekera kwa phage kothandizidwa: Kukulitsa phage ya M13 + kuyeretsa. (5) Kupulumutsa laibulale yowonetsera Phage	Masabata 3-4
Gawo 4: Kuwunika laibulale ya ma antibodies (magawo atatu)	(1) Kuwunika kwa nthawi zonse kwa magawo atatu (kuwunika kwa magawo olimba): kuwunika kuthamanga kwa magazi kuti muchotse ma antibodies osakhala enieni momwe mungathere. (2) Kuchulukitsa kwa bacteriophage imodzi yosankhidwa + IPTG yomwe yachititsa + kuzindikira kwa ELISA kwa ma clones abwino. (3) Ma clones onse abwino adasankhidwa kuti ayesedwe motsatira majini.	Masabata 4-5

Ubwino wa Ukadaulo wa Kuwonetsera kwa Phage

Alpha Lifetech ili ndi chidziwitso chochuluka pakupanga ma antibodies. Kwa zaka zambiri, Alpha Lifetech yakhazikitsa njira yopangira ma antibodies ambiri.

Ntchito Zothandizira

Tikhoza kupereka ntchito zosiyanasiyana zomanga laibulale ya ziweto ndi ntchito zowunikira ma antibodies achilengedwe malinga ndi zosowa za makasitomala.

Zolinga Zambiri

Pali mautumiki angapo opezera ma antibodies omwe alipo: mapuloteni, ma peptide, mamolekyu ang'onoang'ono, ma virus, mapuloteni a membrane, mRNA, ndi zina zotero.

Ma Vekitala Angapo

Ntchito yomanga laibulale yopangidwa ndi munthu payekha, titha kupereka ma vector osiyanasiyana a bacteriophage kuphatikiza PMECS, pComb3X, ndi pCANTAB 5E, ndikusintha malinga ndi zosowa za makasitomala.

Nsanja Yokhwima

Kuchuluka kwa malo osungira kumatha kufika 10 ^ 8-10 ^ 9, kuchuluka kwa ma antibodies onse kuli pamwamba pa 90%, ndipo mphamvu ya ma antibodies yomwe imapezeka poyesa nthawi zambiri imakhala pamlingo wa nM pM.

UTUMIKI Wogwirizana

Njira Zambiri Zopangira Ma Antibody

Utumiki Wopanga Ma Antibody a Monoclonal

Pulatifomu ya Ukadaulo wa Hybridoma

Pulatifomu Yosankhira Maselo Awiri Awiri

Pulogalamu Yopangira Ma Antibody Yowonetsera Phage Display

Kupanga kwa ma antibodies a monoclonal - Alpha Lifetech

Utumiki Wopanga Ma Antibody a Monoclonal

Tikhoza kupereka ntchito zapamwamba kwambiri, zoyera kwambiri, komanso zapadera kwambiri zopanga ma antibodies a monoclonal, kuphatikizapo kupanga ma antibodies a monoclonal a mbewa ndi ma antibodies a kalulu a monoclonal.

Pulatifomu ya Ukadaulo wa Hybridoma

Kuphatikizapo pulogalamu ya katemera, ntchito zokonzekera ma antibodies, kuyeretsa ma antibodies, kutsata ma antibodies ambiri, kutsimikizira ma antibodies, ndi zina zotero.

Pulatifomu Yosankhira Maselo Awiri Awiri

Alpha Lifetech ili ndi ubwino pa nthawi yoyezetsa magazi ndi kupeza ma antibodies apamwamba kwambiri. Ikhoza kupereka kapangidwe ka ma antigen, kupanga, ndi kusintha, chitetezo cha nyama, kuyesa kuchuluka kwa maselo a B amodzi, ndi kutsata maselo amodzi.