Mau Oyamba a Makalata Othandizira Oteteza Anthu Opangidwa ndi Kachitidwe

Laibulale ya ma antibody yopangidwa, yomwe imadziwikanso kuti laibulale ya De novo, ndi njira yopangira yomwe imagwiritsa ntchito njira monga DNA synthesis kapena phage display popanga ndikupanga madera athunthu a antibody variable, kuphatikiza madera a framework ndi ma CDR, popanda kudalira malaibulale a antibody opanda nzeru.

Laibulale ya ma antibody yopangidwa mwa semi-synthetic imapangidwa mwa kuphatikiza ma laibulale a ma antibody omwe amapezeka mwachilengedwe ndi mitundu yosiyanasiyana yopangidwa. Nthawi zambiri imaphatikizapo kupanga ma oligonucleotide a DNA kuti apange magulu osiyanasiyana ozindikira ma complementary determining regions (CDRs), omwe amaphatikizidwa ndi constant antibody framework yochokera ku magwero achilengedwe monga maselo a B a anthu kapena nyama. CDR yopangidwayo imayambitsa mitundu yosiyanasiyana mu laibulale, zomwe zimapangitsa kuti ma antibodies azitha kuyang'ana ma epitopes osiyanasiyana. Laibulale ya ma antibody yopangidwa mwa semi-synthetic imapereka mgwirizano pakati pa mitundu yosiyanasiyana ya chitetezo cha mthupi ndi mitundu yosiyanasiyana yolamulidwa yomwe imapezeka kudzera mu njira zopangira.

Kusiyana pakati pa malaibulale a ma antibodies osadziwa zambiri ndi opanga kumadalira komwe majini a immunoglobulin amachokera. Pakadali pano, kusiyana pakati pa laibulale ya ma antibodies osadziwa zambiri ndi opanga pang'ono kuli m'chakuti yoyamba imakhala ndi gawo la magawo a ma antibodies osadziwa zambiri, monga unyolo wopepuka kapena unyolo wolemera, ndipo imodzi yokha ndi yomwe imapangidwa mu vitro; pomwe yopangidwayo imachokera kwathunthu ku kupanga kochita kupanga ndi PCR mu vitro.

Alpha Lifetech ingapereke

Alpha Lifetech Inc.ikhoza kupereka laibulale ya ma antibodies yopangidwa pang'ono/yopangidwa kuchokera ku nyama ndi anthu kutengera nsanja yathu yaukadaulo yopezera ma antibodies. Ndi zaka zambiri zokumana nazo pakusintha majini ndi kumanga laibulale, malaibulale opangidwa amatha kumangidwa omwe amatha kupanga ma antibodies opangidwa ndi mphamvu komanso apadera kuposa mphamvu ya ma antibodies achilengedwe.Alpha Lifetech'sAkatswiri akunyadira kulengeza kuti tikhoza kutsimikizira kuti padzakhala kupambana kwakukulu pakupanga laibulale ya ma antibodies yokhala ndi ma clones odziyimira pawokha a 10^8 - 10^10.

Alpha Lifetech Inc.ikunyadira kupereka ntchito zonse zomanga laibulale ya antibody kuti ikwaniritse zosowa zosiyanasiyana za ofufuza padziko lonse lapansi, kuphatikizapo scFv, Fab, VHH antibody, ndi malaibulale osinthidwa. Cholinga chathu ndikumvetsetsa ndikukwaniritsa zosowa za makasitomala osiyanasiyana ndikuthandizira pamavuto aliwonse omwe akubwera komanso omwe akubwera pantchito yofufuza.

Ntchito Yomanga Malaibulale Opangidwa ndi Ma Antibody

Kapangidwe
Gawo lokonzekera limaphatikizapo kusankha ma framework a antibody ndi madera ozindikira mgwirizano (CDRs) kutengera ma antibodies omwe alipo kapena deta ya kapangidwe kake. Ma antibodies a CDR opangidwa amathanso kupangidwa kuti ayambe kugwira ntchito zinazake kapena kuwonjezera mphamvu zomangira.

Kupanga
DNA yopangidwa yomwe imalemba ma antibody omwe adapangidwa, kuphatikiza madera onse a framework ndi ma CDR, imapangidwa pogwiritsa ntchito njira zamakemikolo kapena ma enzyme.

Msonkhano
Zidutswa za DNA zopangidwa zimasonkhanitsidwa kukhala ma vector owonetsera ma antibody pogwiritsa ntchito njira monga PCR, ligation, kapena Gibson assembly. Ma vector awa amatha kulowetsedwa mu machitidwe owonetsera ma antibody monga mabakiteriya, yisiti, kapena maselo a nyama kuti apange ma antibody.

Kuwunika ndi Kusankha
Malaibulale opangidwa ndi ma antibodies amafufuzidwa ndikusankhidwa kuti apeze ma antibodies omwe ali ndi makhalidwe omwe amafunidwa pogwiritsa ntchito njira zowunikira zapamwamba. Izi zitha kuphatikizapo njira monga chiwonetsero cha phage, chiwonetsero cha yisiti, kapena chiwonetsero cha ribosome, kutengera mtundu wa laibulale ndi momwe imagwiritsidwira ntchito.

Ntchito Yomanga Malaibulale Oletsa Kupha Anthu Osagwiritsa Ntchito Mankhwala

Alpha Lifetech Inc.Amapatsa makasitomala kapangidwe kake kamodzi kokha komanso ntchito yopangira ma antibody libraries polumikiza ma V-gene repertoires a heavy and light chain V-gene mu phage vector pogwiritsa ntchito Cre-lox site-specific recombination system kuti apange repertoire ya Fabs yomwe ikuwonetsedwa pa phage yokhala ndi ma clones 6.5 × 10^10. Laibulaleyi idapanga Abs motsutsana ndi ma antigen ambiri, ena okhala ndi nanomolar affinities. Kupanga kwa laibulale ya antibody ya anthu kumadalira ukadaulo wowonetsera wa M13 phage (womwe wawonetsedwa pachithunzi pansipa).