Peptide Library Construction Platform

Alpha Lifetech offers comprehensive phage peptide library construction services to support peptide discovery and screening. Our platform includes T7 phage display peptide libraries and M13 phage display peptide libraries, including linear 7-, 9-, 10-, 12-, and 15-mer peptides, as well as cyclic 7- and 12-mer peptides libraries. We also provide customized peptide library design and construction services tailored to specific research objectives, along with custom peptide synthesis to meet diverse experimental requirements.

Phage display technology enables the presentation of exogenous peptides on the surface of bacteriophages while preserving both peptide functionality and phage infectivity. Each recombinant phage displays a unique peptide sequence, and together these variants form a highly diverse peptide library for screening target-specific binders (Learn More). Phage display peptide libraries have become indispensable tools for peptide discovery and have been widely applied in epitope mapping, ligand identification, antibody characterization, drug discovery, vaccine development, and other biomedical research.

Introduction to The Peptide Library Construction

Peptide library construction is based on phage display technology, in which randomized peptide-coding DNA sequences are fused to phage coat protein genes, enabling the corresponding peptides to be displayed on the surface of bacteriophages. This system establishes a direct linkage between genotype (encoding DNA) and phenotype (displayed peptide), allowing efficient selection of target-binding peptides through iterative biopanning.

As a biosynthetic peptide discovery platform, phage display combines molecular cloning and in vivo amplification to generate highly diverse peptide repertoires. Alpha Lifetech provides robust peptide library construction services, including linear and cyclic formats with different peptide lengths (7, 9, 10, 12, and 15-mer), as well as fully customized library design for specific research and application needs.

Application of Peptide Library Construction and Screening

Phage-displayed peptide libraries are widely used for affinity-based screening of functional peptides through solid-phase or solution-phase (liquid-phase) panning strategies. These approaches enable efficient enrichment of high-affinity binders from large randomized peptide populations.

Key applications include epitope mapping of immune targets, identification of receptor- or protein-binding peptides, discovery of non-protein target ligands, and selection of tissue- or cell-specific targeting peptides. In addition, selected peptides are increasingly used in the development of peptide-drug conjugates and targeted delivery systems, supporting drug discovery and translational biomedical research.

Phage Display Peptide Library Process

Fig.1 Phage Display Peptide Library Constrution Platform

Phage Display Peptide Library Construction Workflow

Process	Service Content	Timeline
1. Nucleotide Sequence Synthesis	Random synthesis of nucleotide sequences of customer specified length	2-3 weeks
2. Polymerization and Digestion	Polymerization and digestion of the synthesized DNA sequence	1 day
3. Electroconversion Libraries Construction	ER2738 host bacteria were constructed, and then E. coli was transformed with the well-constructed recombinant plasmid	1 week
4. Peptide Library Size and Diversity Detection	Detection of peptide library size by colony counting and peptide library diversity by sequencing	2-3 days
5. Phage Display Peptide Library Construction	By infecting the phage packaging peptide library	1 week