Phage display 7 peptide Library Construction Service

Alpha Lifetech offer a range of phage display peptide library construction services such as M13, T4, T7 or λ phages for our customers, with M13 phage display being our commonly used method. We are capable of constructing linear peptide libraries and structurally stable cyclic peptide libraries. We can tailor peptide libraries with different structures containing 5-25 amino acids. In addition to linear 7-mer peptide libraries, we also construct cyclic 7-mer peptide libraries, linear 12-mer peptide libraries, linear 15-mer peptide libraries, linear 9-mer peptide libraries and cyclic 9-mer peptide libraries.

7 Peptide Libraries construction Service

There are two main methods for constructing 7 peptide libraries provided by Alpha Lifetech: organic synthesis method and genetic engineering method.

Organic synthesis

Using solid phase peptide polypeptide synthesis technology, directly synthesis of 7 peptides containing various possible sequences. The synthesis begins with a dipeptide and gradually increases the length of the peptide chain and the types of amino acids replaced until the required 7 peptide lengths are reached. Each vector contains only one sequence of 7 peptides. The method of organic synthesis is simple and rapid. However, it can not be amplified, and screening is more troublesome, and fluorescent labeling or ELISA detection means are needed, which is a large workload.

Genetic engineering method

A set of oligonucleotide mixture (small peptide gene mixture) synthesized by gene cloning technology was cloned into the phage genome and expressed on the phage surface in the form of fusion protein. By screening phages that bind to target proteins, 7-peptide sequences with high affinity and specificity can be identified. Through genetic engineering, large-scale peptide libraries can be constructed, and it is easy to expand and screen.

Phage Display 7 Peptide Library Construction Process

Fig.1 Phage Display 7 Peptide Library Constrution Service

Peptide Library Screening Process

Phage library screening is a technology that fuses foreign proteins or peptides with phage shell proteins, displays them on the phage surface and maintains a specific spatial conformation, and then uses specific affinity to screen specific proteins or peptides.

Fig.1 Phage library screening process. (Reference source: Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries)

Phage Display Peptide Library Construction Workflow

Process	Service Content	Timeline
1. Nucleotide Sequence Synthesis	Randomly synthesize nucleotide sequences of 7 peptides	2-3 weeks
2. Polymerization and Digestion	Polymerization and digestion of the synthesized DNA sequence	1 day
3. Electroconversion Libraries Construction	ER2738 host bacteria were constructed, and then E. coli was transformed with the well-constructed recombinant plasmid	1 week
4. Peptide Library Size and Diversity Detection	Detection of peptide library size by colony counting and peptide library diversity by sequencing	2-3 days
5. Phage Display 7 Peptide Library Construction	By infecting the phage packaging linear 7-peptide librarypeptide library	1 week