Why Choose Our Recombinant Protein?

High Purity: Our recombinant proteins are produced using advanced technology to ensure superior purity and eliminate contaminants that could compromise their efficacy.

High efficiency: Through precise manufacturing processes, our recombinant protein products have excellent efficacy and can achieve maximum effectiveness in a wide range of applications.

Stable quality: Each batch of our recombinant proteins undergoes strict quality control measures to ensure stable quality and performance, providing researchers and scientists with reliable results every time.

Cost-Effective: We understand the importance of delivering value without compromising on quality. By choosing Alpha Lifetech, you gain access to high-quality recombinant proteins at competitive prices, further extending your research budget without sacrificing experimental integrity.

Recombinant Protein List

Recombinant protein Products

Alpha Lifetech has over 2000+ recombinant protein products and successfully collaborates with thousands of global enterprise clients every year to provide them with solutions. Unlock the potential of recombinant protein expression technology with our high-quality recombinant protein products and services. Contact us today to accelerate your projects with recombinant protein expression services and recombinant protein products.

Recombinant Protein For Cancer

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Custom Recombinant Protein Production Service

Over the last 30 years, there have been considerable advances in the technologies for expressing recombinant proteins. Alpha Lifetech Inc. provides four main protein expression systems to produce recombinant proteins: Bacterial, yeast, insect cells, and mammalian systems. Alpha Lifetech Inc. provides a portfolio of recombinant proteins from different hosts, and different species, the products can be used for research and diagnostic reference purposes. Recombinant proteins include enzymes, growth factors, cytokines, Fc receptor proteins, immune checkpoint proteins, and membrane proteins. These recombinant proteins can be used for a variety of applications, including cell culture, bioassays, and in vitro diagnostics.

Recombinant Protein Expression System Comparison

Expression Systems

Bacterial

System

Yeast

System

Baculovirus

-insect

System

Mammalian

System

Advantage

Lower cost

Time-saving

Cost-effective

industrial scale-up

Fast growth

post-translational modifications

Clear genetic background

Simple cultivation conditions

High security

High-efficiency expression

post-translational modification

Low toxicity

Strong security

Stable growth characteristics

Easy to cultivate and transfect

High-efficiency expression

Disadvantage

Protein degradation

Toxicity issues

Endotoxin contamination

Protein insolubility

Lower yields

Lower protein yields Limited suitability for the expression of large and highly hydrophobic proteins.

Limited capability to produce proteins with high molecular weights; Less efficient at producing membrane proteins

Higher cost

Longer production timelines

Complex culture requirements

Application

Structural studies

Production of soluble, small proteins

Industrial enzyme production

Functional studies

complex secreted proteins;

Vaccine development

Drug discovery

Protein-protein interaction studies

FAQ Of Recombinant Protein Products

1. How do I apply for a quotation for bulk products?

Determine the specific product or products you are interested in purchasing in bulk. This may include recombinant proteins, antibodies, or other biological reagents. Please Contact us at info@alpha-lifetech.com
2. How can I obtain COA of the recombinant protein that I received?

Please contact us via email info@alpha-lifetech.com for detailed information about the product.
3. What is a recombinant protein?

A recombinant protein is a protein that is produced using recombinant DNA technology, typically by expressing a gene of interest in a heterologous host organism, such as bacteria, yeast, or mammalian cells. This technology allows for the production of proteins that are not naturally abundant or difficult to obtain from their native sources. Alpha Lifetech can provide recombinant protein products.
4. What are the shipping conditions for recombinant proteins?

Recombinant proteins are often shipped on dry ice (−78.5℃) or with gel packs to maintain a frozen or cold temperature during transit.If the protein is stable at refrigerated temperatures (2-8℃) for short periods, it may be shipped at 2-8℃with gel packs to maintain a cool temperature during transit.For proteins that are stable at room temperature or refrigerated temperatures for longer periods, shipping at ambient temperature (15-25℃) may be feasible. Alpha Lifetech sends you protein products according to strict requirements.
5. How is the quality of recombinant proteins ensured?

The quality of recombinant proteins is ensured through rigorous quality control measures, including protein characterization, purity assessment, and functional testing. Analytical techniques such as SDS-PAGE, HPLC, mass spectrometry, and bioassays are used to assess the quality and integrity of recombinant proteins.

FAQ Of Recombinant Protein expression service

1. What are the common host organisms used in recombinant protein expression?

Common host organisms for recombinant protein expression include bacteria (e.g., Escherichia coli), yeast (e.g., Saccharomyces cerevisiae), insect cells (e.g., Spodoptera frugiperda), and mammalian cells (e.g., Chinese hamster ovary cells). Each host organism has its advantages and disadvantages in terms of protein expression levels, post-translational modifications, and scalability. Alpha Lifetech has four major protein expression systems for you to choose from that are suitable for your project, helping you efficiently complete the project.
2. What factors should be considered when choosing a recombinant protein expression system?

Factors to consider include protein size and complexity, desired post-translational modifications, expression levels, protein solubility, and downstream processing requirements. Additionally, cost, ease of use, and regulatory considerations may influence the choice of expression system.
3. What are the advantages of using bacteria for recombinant protein expression?

Bacteria, such as Escherichia coli, are commonly used for recombinant protein expression due to their rapid growth, ease of manipulation, and high protein expression levels. They are particularly suitable for producing small to medium-sized proteins that do not require post-translational modifications.
4. What are the advantages of using mammalian cells for recombinant protein expression?

Mammalian cells, such as Chinese hamster ovary (CHO) cells, offer advantages for producing complex proteins that require proper folding, post-translational modifications, and secretion. Mammalian expression systems can produce proteins with human-like glycosylation patterns and are often used for therapeutic protein production.
5. What are some challenges associated with recombinant protein expression?

Challenges include protein misfolding, aggregation, low expression levels, toxicity to the host organism, and difficulties in achieving proper post-translational modifications. Optimization of expression conditions and selection of appropriate host organisms can help address these challenges.
6. How can I purify the recombinant protein?

Protein purification methods vary depending on the protein's properties and the expression system used. Common techniques include affinity chromatography, ion exchange chromatography, size exclusion chromatography, and various forms of protein-specific purification methods (e.g., His-tag purification, GST-tag purification). Multiple purification steps may be required for obtaining highly pure proteins. Alpha Lifetech has multiple protein purification systems to help you obtain high-purity proteins.
7. What are the common methods used for recombinant protein purification?

Common methods for protein purification include chromatography (e.g., affinity chromatography, ion exchange chromatography, size exclusion chromatography), precipitation, ultrafiltration, and dialysis. Each method exploits different physical or chemical properties of proteins to achieve purification.
8. How do I choose the appropriate purification method for my protein?

The choice of purification method depends on various factors, including the size, charge, solubility, and specific properties of the target protein. Consideration should also be given to the scale of purification, the desired purity level, and the downstream applications of the purified protein.
9. How are purity and yield assessed during protein purification?

Purity and yield are typically assessed using analytical techniques such as SDS-PAGE, western blotting, UV absorbance spectroscopy, and Bradford or BCA protein assays. These techniques provide quantitative and qualitative information about the composition of the purified protein sample.
10. What to do if the target protein is insoluble?

Adjusting the growth conditions of the host organism, such as temperature, induction time, inducer concentration, and culture medium composition, can sometimes promote proper protein folding and increase solubility. Adding fusion tags or solubility tags to your target protein can improve solubility. Common fusion tags include glutathione S-transferase (GST), maltose-binding protein (MBP), and small ubiquitin-related modifier (SUMO).

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Customer
evaluation

This CD28 protein was of high purity and maintained biological activity in our T-cell proliferation assays. The lyophilized format was easy to reconstitute, and results were consistent across experiments. Would definitely recommend for studies--Human CD28 Protein (ALP64560)

Dr. Michael Lee

Johns Hopkins University

We used this spike protein for ELISA-based neutralization assays and protein-protein interaction studies. The protein was well-folded and showed strong binding to ACE2. Some minor aggregation was noted at high concentrations, but overall, a great product--SARS-CoV-2 Spike RBD Protein.

Dr. Olivia Martinez

Cambridge University

The recombinant GFP protein had strong fluorescence and worked well for in vitro tracking experiments. The purity was high, though the storage buffer required slight modification for our needs. Overall, a product for fluorescence-based applications--Recombinant GFP Protein.

Dr. William Kim

Kyoto University

010203

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5. December. 2018

Why Choose Our Recombinant Protein?

Recombinant protein Products

Custom Recombinant Protein Production Service

Recombinant Protein Expression System Comparison

FAQ Of Recombinant Protein Products

1. How do I apply for a quotation for bulk products?

2. How can I obtain COA of the recombinant protein that I received?

3. What is a recombinant protein?

4. What are the shipping conditions for recombinant proteins?

5. How is the quality of recombinant proteins ensured?

FAQ Of Recombinant Protein expression service

1. What are the common host organisms used in recombinant protein expression?

2. What factors should be considered when choosing a recombinant protein expression system?

3. What are the advantages of using bacteria for recombinant protein expression?

4. What are the advantages of using mammalian cells for recombinant protein expression?

5. What are some challenges associated with recombinant protein expression?

6. How can I purify the recombinant protein?

7. What are the common methods used for recombinant protein purification?

8. How do I choose the appropriate purification method for my protein?

9. How are purity and yield assessed during protein purification?

10. What to do if the target protein is insoluble?

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