Serivisi yo gukura kw'abarwayi bangiza umubiri

Alpha Lifetech Inc.ishobora gutanga serivisi yo gukura kw'umubiri hakoreshejwe ikoranabuhanga rya mutation no gutoranya. Ubusanzwe dukoresha scFv nk'uburyo bwa antibody mu bikorwa byo gukura kw'umubiri hamwe na sisitemu ya monovalent display phagemid yakoreshejwe mu kugabanya ingaruka z'ubushyuhe mu gihe cyo gusuzuma antigen-binding. Itsinda ryacu ry'inzobere rishobora gutanga serivisi zo gukura kw'umubiri ku barwayi ba antibody bo mu bwoko bumwe.

Alpha Lifetech Inc.Ifite ishema ryo gutanga serivisi zirambuye kugira ngo ihuze n'ibyifuzo bitandukanye by'abakiriya ku isi. Intego yacu ni ukumva no guhaza ibyifuzo by'abakiriya batandukanye no gufasha mu bibazo byose bizaza n'ibivuka mu bushakashatsi.

Gukura mu buryo bw'ubucuti (Affinity Maturation) ni iki?

Gukura kw'uturemangingo ni inzira aho uturemangingo tw’umubiri duhinduka inshuro nyinshi kandi tugatoranywa kugira ngo twongere ubufatanye bwatwo ku turemangingo twihariye. Uturemangingo twa B, cyane cyane tuboneka mu gice cy’uturemangingo tw’umubiri, tugenda duhinduka cyane mu mubiri kandi tugatoranywa bitewe n’uturemangingo twa T na follicular dendritic.
Mu gihe cyo gukura kw'uturemangingo, uturemangingo twa B dufite immunoglobulin nkeya zishyirwa mu bikorwa n'udukoko duhura natwo hanyuma zigakwirakwira no gutandukana. Binyuze mu gukura kw'uturemangingo twa somatic hypermutation, haboneka uburyo bwo gusimbuza nucleotide mu gace gahinduka k'uturemangingo twa immunoglobulin, biganisha ku gukora ubwoko butandukanye bw'uturemangingo. Nyuma yo guhinduka kw'uturemangingo twa somatic, hatoranywa antigen ku turemangingo twa B dufite immunoglobulin yahinduwe. Uturemangingo twa B dukora antibodies zifite immunoglobulin nyinshi zishyirwa mu mwanya wazo kandi zigahabwa ubushobozi bwo kwiyongera, mu gihe uturemangingo twa B dufite immunoglobulin yagabanutse cyangwa idahinduka tugenda duhinduka. Iyi gahunda isubiramo yo guhinduka no guhitamo ituma uturemangingo twa B dukora clones, bigatuma uturemangingo twa antigen dutangira kwiyongera buhoro buhoro.

Serivisi yo gukura kw'abarwayi bangiza umubiri

Ibyiza by'ingenzi by'uburyo bushingiye kuri PCR ni uko impinduka ziba zigamije neza igice cyazamuwe; igipimo cy'amakosa cyoroshye kugenzura kandi uburyo bworoshye gushyiraho kandi ntibukoresha imiti ihumanya. Birazwi neza ko polymerase ya Taq DNA ikora ADN nk'iyindi mu buryo budakwiye, ahanini bitewe nuko nta gikorwa cyo gukosora hagati ya metero 3 na metero 5.Alpha Lifetech'sUburyo bwo gukora antibody bukoresha uburyo bwa PCR bushobora kwibasira uturere twa CDR mu gihe cyo kubaka isomero rito. Kandi akenshi dukora impinduka mu myanya itunguranye ku bice byose bya VH na VL, kugira ngo twongere ubwinshi bw'uturemangingo tw'isomero rito. Nyuma yo kubaka isomero rya antibody gene rihinduka hakoreshejwe PCR ihura n'ibibazo, guhitamo ubwoko bwinshi bw'ubufatanye (ubufatanye bw'uturemangingo twa scFv bushobora kugera kuri 10^8 - 10^10 M) bikorwa hakoreshejwe uburyo bwo gukaraba cyangwa kuri antigen idakora hamwe n'uburyo bwo gukaraba bukozwe neza kugira ngo hatoranywe ibintu bidahuye n'ubushobozi.
  
Abahanga mu bya siyansi muriAlpha Lifetech Inc.Koresha ubwoko bwa Escherichia coli mutator mutAD5TM nk'imwe mu ngamba nyinshi zo guhindura imiterere y'uturemangingo kugira ngo ushyireho impinduka zidasanzwe, bityo uhindure uburyo uduce twa antibody twiyongera. Imiterere y'uburyo bwo guhitamo ishobora guhindurwa ku duce twa antibody hamwe n'umusaruro wiyongera. Imiterere y'imikurire mu turemangingo twa Escherichia coli mutator ishobora guhindurwa kugira ngo hashyirweho impinduka imwe ya random point kuri kilobase ya ADN, hafi kimwe n'impinduka imwe ya kodoni kuri buri gice cya scFv. Nyuma y'ingendo nyinshi zo guhindura imiterere, kwerekana no guhitamo, dushobora kunoza uburyo bwo guhindura imiterere y'uturemangingo kuva kuri 10^5 - 10^6 M kugeza kuri 10^8 - 10^10 M.

Kunoza ingamba zo gukura mu mibanire

Imyanya imwe n'imwe y'uturemangingo tw'umubiri ishobora guhindurwa ku buryo butandukanye (nk'uburyo bwuzuye bwo gukora ibintu hamwe na aside amine 20 zose cyangwa uburyo bwo gukora ibintu bitandukanye hamwe na aside amine zatoranijwe ku kigero gihamye) kugira ngo twongere uburyo bwo gukora ibintu. Dufite ingamba ebyiri zo kunoza uburyo bwo gukora ibintu bitandukanye: icya mbere, impinduka zishyirwa ku myanya igenzurwa mu turere duhuza ibintu (CDRs) binyuze mu guhindura ibintu mu buryo buyobowe n'ahantu; icya kabiri, impinduka zishyirwa mu turere twose twa V-coding binyuze mu guhindura ibintu mu buryo butunguranye. Mu gukoresha ubu buryo bubiri, uburyo buzigamye bwa aside amine cyangwa agace kose k'umuyoboro w'umubiri bizasimburwa n'izindi aside amine, zihujwe n'ikoranabuhanga ryacu ryo gusesengura ibitabo bya peptide, zishobora kongera cyane uburyo bwo gukora ibintu bitandukanye ku rwego rwo hejuru cyane.

Icyerekezo cya Phage-Antibody Affinity

Iyo isomero rya scFv mutant rimaze kubakwa, hari ingamba ebyiri zo gusuzuma: biopanning na solid-antigen sorting. Mu ya mbere, hakoreshwa icyiciro gito cya substrate kugira ngo hamenyekane antibody ifite affinity high-affinity, kuko mutants ifite affinity low-affinity yakurwamo, hagasigara uduce twa phage dufite avidity high-avidity. Ingamba ya kabiri ikoresha antigen ifite label mu gisubizo, guhitamo hashingiwe ku gipimo cy’uburinganire (Kd), no guhitamo hashingiwe ku buryo bwo gufatanya, ubu buryo bwo guhitamo bugaragaza ubwoko bwa antibody bufite Kd nziza.

Niba ufite ikibazo icyo ari cyo cyose, twandikire igihe icyo ari cyo cyose.