Icyiciro cyo kwerekana serivisi Intangiriro

Icyiciro cyerekana, nkubuhanga bwa molekuline bushingiye ku ihindagurika ry’imiterere ya ADN ya fage, byahindutse igikoresho gikomeye cya molekile cyo kubyara molekile zirwanya intego zihariye zerekana ibyo bice hejuru yicyiciro bityo ugahitamo ibice bya peptide / antibody hamwe nibintu byihariye bihuza biturutse kumubare munini wibitabo (amasomero).

Alpha Lifetech ifite uburambe bwimyaka icumi mugutezimbere icyiciro cya antibodiyite kandi irashobora gutanga isomero rya antibody yumwuga yo kubaka no gusuzuma serivisi kubakiriya bacu. Ukurikije ibyo abakiriya bakeneye, turashobora gutanga uburyo butandukanye bwamasomero ya antibody, nkamasomero yubudahangarwa, amasomero kavukire, amasomero yubukorikori hamwe nububiko bwibitabo. Ikarita yerekana isomero rya tekinoroji, ihuza genes ya antibody hamwe na genes za proteine za fage nka M13, ni tekinoroji yo gutegura antibody ya recombinant kandi ikoreshwa cyane mugutezimbere antibodi zihariye.

Alpha Lifetech izakingira alpaca, llama, alpaca, na sharke, nibindi, kugirango imikorere yabo igere kuri 10 ^ 5. twohereze raporo yikizamini cyo gukora neza kubakiriya kugirango tumenye niba amakuru ari ukuri. Amasomero kavukire afite ibyiza byuburozi buke hamwe nubudahangarwa buke. Alpha Lifetech ifite umubare munini w'amasomero ya antibody ya kijyambere, ashobora gukoreshwa mu buryo butaziguye mu gusuzuma antibody atakingiwe inyamaswa, bityo bikagabanya igihe cy'umushinga, bigatera imbere igeragezwa ry'umukiriya, kandi byemeza ko antibody ikora neza.

Igishushanyo 1: Ihame ryerekana icyiciro

Sisitemu Yerekana Sisitemu

Amakuru yingenzi ya sisitemu yo kwerekana icyiciro cya M13 / T4 / T7 yerekanwa mu mbonerahamwe ya 1.

Imbonerahamwe 1 Ibyiciro bitandukanye byerekana ibinyabiziga nibiranga
	M13	T4	T7
Ingano ya Genome	6407 bp	168895 bp	39937 bp
Erekana poroteyine	pVI, pIII na pVIII	SOC na HOC	gp10B
Erekana Ingano	> 110 kDa ni pIII
Erekana Ingano
Erekana Ubucucike			Kopi 415
Erekana Ubucucike			Kopi 415
Ubuzima	Lysogeny	Lytic	Lytic

Alpha Lfetech Irashobora Gutanga

Icyiciro Cyerekana gihuza genotype na phenotype murimwe, ikomatanya guhitamo hamwe no kongera imbaraga hamwe nubushobozi bukomeye bwo gusuzuma. Alpha Lifetech irashobora gutanga serivisi zitandukanye kubikorwa bya fage yerekana antibody.
Serivisi nyamukuru zirimo: VHH yububiko bwibitabo bwa antibody, urubuga rwibitabo rwa scFv antibody, urubuga rwibitabo rwa Fab antibody, urubuga rwo kubaka isomero rya fage, urubuga rwo kwerekana isomero rya fage hamwe na serivisi ishinzwe ubumuntu hamwe nizindi serivisi.

Igishushanyo 2 ： Icyiciro cyerekana serivisi ziterambere

Icyiciro Cyerekana Antibody Iterambere

Turashobora gukingira inyamaswa hamwe na poroteyine zigaragara muri laboratoire yacu cyangwa zitangwa nabakiriya bacu, harimo proteine zitandukanye za macromolecular, peptide na proteine za membrane. Binyuze mu gupima imikorere ya ELISA, abashakashatsi bacu bakoze kwigunga kwa PBMC no gukuramo RNA mumaraso ya alpacas nibisubizo byiza byo gukingira. Mugihe cyo guhindura transcription hamwe na amplification ya gene, twubatse gene kuri pMESC, pComb3XSS cyangwa pCANTAB 5E fage vectors. Hamwe na electrotransformation ya TG1 E. coli ingirabuzimafatizo, isomero rya antibody ya fage antibody ifite ubushobozi bwibitabo burenze 10 ^ 9 irashobora kuboneka. Amasomero ya Antibody afite ubushobozi bwibitabo bunini afite ubudasa bwiza kandi bufasha kubona antibodies zifitanye isano. Kugirango tumenye neza isomero rya antibody, tuzaranga ubushobozi bwibitabo nigipimo cyo kwinjiza isomero. Turangije dukurikirana amasomero kugirango duhe abakiriya bacu 30-50 zikenewe. Noneho, dukora fage yerekana isomero ryerekana isomero, Icya mbere, antigen ihagarikwa kuri microplate ya polypropilene, kandi binyuze mumuzingo 3-5 wo gusuzuma, fage-antibodies zifite ubushobozi buke bwo guhuza zivanwaho, hanyuma clone yihariye ihuza antigen iragumana. Kugaragaza fage, uburyo bwa ELISA bukoreshwa kugirango burangire hamwe na clone nziza, mugihe kumasemburo yerekanwe, uburyo bwa FACS bukoreshwa kugirango selile zigaragaze ibice bya antibody recombinant ukoresheje antigens yanditseho. Binyuze mu gusuzuma, dutegura antibodies zifite sensibilité nyinshi, umwihariko hamwe no gukundana cyane.

Igishushanyo cya 3 Icyiciro cyerekana antibody synthesis

INYUNGU ZA serivisi
Ibyiza bya serivisi

Alpha Lifetech yiyemeje cyane tekinoroji yo kwerekana ibyiciro, kandi irashobora guha abakiriya serivisi nini kandi nziza.

Igihe cyagenwe kandi neza

Isosiyete yacu irashobora gushakisha antibodi zishimishije mugihe gito; turashobora gukora ibizamini bitandukanye byo gusuzuma antibody dukurikije ibyo abakiriya bakeneye.

Ubwiza bwibicuruzwa byiza

Isosiyete yacu ifasha abakiriya gutegura antibodies zifite umwihariko kandi uhamye, byongera umusaruro wibisubizo byubushakashatsi.

Tanga amahitamo atandukanye

Isosiyete yacu irashobora kubaka amoko menshi (abantu, imbeba, urukwavu, alpaca, nibindi) amasomero ya antibody kandi irashobora kubaka ubwoko butandukanye (scfv, Fab, VHH, nibindi) byububiko bwibitabo bya antibody.

Ubushobozi bunini

Isomero ryacu rya antibody rifite ubushobozi bunini bwa antibodi zirenga 10 ^ 9

Ibibazo-Rusange ibibazo rusange byerekana fage

icyiciro cyerekana ibibazo ◢

Ikibazo.
Ikoranabuhanga ryerekana ibyiciro ni iki?

A.
Ikoreshwa rya tekinoroji yerekana ni ugushyiramo gene y’amahanga mu mwanya wihariye wa poroteyine ya fage capsid, no kwerekana gene y’amahanga hejuru ya fage bitagize ingaruka ku mikorere isanzwe ya fage. Nyuma yo kuzenguruka inshuro nyinshi, poroteyine yagenewe ifite umwihariko hamwe n’ibikorwa biologiya bihanitse yarabonetse.
Ikibazo.
Ni izihe nyungu zo kwerekana tekinoroji?

A.
Amasomero ya antibody afite ubushobozi bwinshi arashobora kubyara hakoreshejwe ubu buhanga. Kugenzura inzinguzingo ngufi, inzira yimikorere iroroshye, kandi urutonde rwibisabwa ni runini, rwemerera kumenya urutonde rwiza rwa peptide cyangwa proteyine. Mubikorwa bifitanye isano na virusi, abashakashatsi barashobora kuvumbura antibodies zihuza epitopes zikomeye za virusi mugusuzuma amasomero manini ya antibody. Iyi nzira irashobora kubangamira uburyo bwo kwandura virusi, kwinjira, kwigana, cyangwa kwirinda indwara, bityo bigashyiraho umusingi wo kuvura indwara.
Ikibazo.
Ni irihe tandukaniro riri hagati ya tekinoroji ya hybridoma na tekinoroji ya fage?

A.
Icyiciro cya tekinoroji yerekana ikoreshwa cyane kuruta tekinoroji ya hybridoma, igarukira gusa kubyara antibodi nkeya zirwanya immunogène. Ibinyuranye, tekinoroji ya fage irashobora kwerekana isomero rya antibody yose ikomoka kumoko atandukanye yumubiri.
Ikibazo.
Ni ubuhe buryo bukoreshwa muburyo bwa tekinoroji yo kwerekana?

A.
Ikoreshwa rya tekinoroji yerekana ibyiciro byinshi, harimo gusuzuma ibyakiriwe na mikorobe itera indwara, kwiga imikoranire ya poroteyine, gukurikirana ihererekanyabubasha hagati ya selile, gusuzuma indwara z’inyamaswa, no guteza imbere inkingo, mu zindi nzego.
Ikibazo.
Icyizere cya tekinoroji yo kwerekana?

A.
Icyiciro cya tekinoroji yerekana ibyiza byinshi kandi birashobora gukoreshwa mubice bitandukanye. Nyamara, ikibazo cyibanze mugutezimbere antibodiyide zikoresheje uburyo bwo kwerekana fage nuburyo bugoye hamwe nigiciro kijyanye nibikorwa. Ubu buryo busaba guhuza tekinoloji y’ibinyabuzima ya molekuline, harimo ihinduka ry’imihindagurikire, gukoroniza, hamwe n’imvugo, hamwe n’isesengura ry’ubudahangarwa, ibinyabuzima, na biofiziki kugira ngo tumenye kandi biranga antibodi zifuzwa.

Ibibazo bifitanye isano na Antigen ◢

Ikibazo.
Nubuhe buryo bwo kwerekana antigen?

A.
Antigen kwerekana ni ihuriro ryingenzi. Uburyo bwo kwerekana antigen burimo cyane cyane kwerekana antigen yerekanwe neza, kwerekana antigen itaziguye, kwerekana antigen binyuze mugupima ingirabuzimafatizo zose, kwerekana antigen binyuze muri liposomes, disiki ya nano-fosifolipide na VLPs.
Ikibazo.
Ni irihe tandukaniro riri muburyo butandukanye bwo kwerekana antigen?

A.
Uburyo bwo gutwikira butaziguye biroroshye, ariko guhindura antigen biroroshye guhinduka, kandi ibikorwa byo gutwikira mu buryo butaziguye biragoye, ariko birashobora gukomeza guhinduka kwa antigen no kunoza imikorere yerekana. Kugenzura selile birashobora kwigana ibintu bifatika muri vivo, ariko hariho ikibazo cyo guhuza bidafite akamaro. Disiki ya Nano-fosifolipide ifite ubunini buto, uburyo bwiza bwo gutanga no guhagarara neza; VLPs ihujwe na antigen cyangwa izengurutswe na antigen, ifite ubudahangarwa bukabije n'umutekano mwiza, kandi ishobora gutera ubudahangarwa bw'umubiri.
Ikibazo.
Ni izihe ngaruka ziterwa na antigen ku bisubizo byerekana tekinoroji ya fage?

A.
Niba kwibandaho ari bike cyane, amahirwe yo guhuza na fage azagabanuka, bikaviramo kugabanuka neza. Ibinyuranye, kwibanda cyane birenze bishobora kongera amahirwe yo guhuza bidafite akamaro, kuzamura ibimenyetso byinyuma, kandi bikabuza kumenya clone nziza.
Ikibazo.
Nigute dushobora kwemeza ko antigen igumana imiterere karemano mugihe cyo gusuzuma?

A.
Uburyo bworoheje bwo gukosora burashobora gukoreshwa kugirango wirinde ubushyuhe bwinshi, aside ikomeye na alkali. Uburyo bwo gutwikira mu buryo butaziguye na sisitemu ya biotin-streptavidin yakoreshejwe mu kugabanya ibyangiritse ku miterere ya antigen. Niba antigen ari poroteyine ya membrane, irashobora kwerekanwa hejuru yutugingo ngengabuzima tudakomeye cyangwa ikoreshwa mu kwigana ibidukikije, nka nano-disiki, kugira ngo ibungabunge poroteyine karemano.
Ikibazo.
Nigute ushobora kugenzura umwihariko wa antibodies zerekanwa na tekinoroji yerekana fagitire kuri antigene?

A.
Usibye ubushakashatsi busanzwe nka ELISA, Western Blotting (WB), na Immunofluorescence (IF), Surface Plasmon Resonance (SPR) hamwe na Bio-Layer Interferometry (BLI) uburyo bwo gutahura burashobora gukoreshwa mugukurikirana uburyo bwo guhuza no gutandukanya antibodi na antigene mugihe nyacyo, bikemerera kumenya ibipimo bifatika.

Ibibazo bifitanye isano n'ubudahangarwa ◢

Ikibazo.
Nibihe bisubizo rusange byubudahangarwa bwinyamaswa muburyo bwa tekinoroji yerekana?

A.
Icyiciro cya fage kirashobora gukoreshwa kugirango habeho antibodiyite nyinshi za monoclonal antibodi yubwoko butandukanye butanga antibody, harimo ariko ntibireba abantu, imbeba, imbeba, inkwavu, inkoko, ingamiya, alpaka, inka, imbwa, intama, inkende, ninyoni.
Ikibazo.
Ni ibihe bintu biranga hamwe nibishobora gukoreshwa mubitabo byibitabo bya antibody bikomoka kumoko yinyamanswa zitandukanye muburyo bwa tekinoroji yo kwerekana?

A.
Tekinoroji yo kubaka amasomero ya antibody yimbeba yarashizweho neza, irahendutse, kandi ifite igihe gito cyo kugerageza, bigatuma ikwirakwira mbere ya antibody nubushakashatsi bwibanze. Antibodiyite z'urukwavu zigaragaza isano ikomeye kandi yihariye, hamwe na epitope yagutse yo kumenyekanisha ugereranije na antibodies. Antibodiyite zabantu zirashobora kuboneka muburyo butaziguye mumasomero ya antibody yumuntu, bityo ukirinda ingaruka zishobora gukingira indwara zishobora kubaho mugihe antibodiyite zidafite umubiri zihabwa umubiri wumuntu. Ubu buryo bufite agaciro gakomeye mubushakashatsi bwibiyobyabwenge niterambere, cyane cyane mugukora antibodies zivura.
Ikibazo.
Ni ubuhe bwoko rusange immunogenogen ikoreshwa muburyo bwa tekinoroji yo kwerekana?

A.
Ubudahangarwa bw'inyamaswa bugabanyijemo poroteyine antigen, polypeptide antigen, aside nucleic antigen hamwe na antigen ifitanye isano na patogene, muri yo harimo antigen ifitanye isano na virusi harimo virusi ya virusi (virusi yuzuye, poroteyine ya virusi, poroteyine idafite imiterere, n'ibindi), antigen ya bagiteri (ikuramo rya bagiteri yuzuye, urukuta rw'uturemangingo, poroteyine isohoka, uburozi, n'ibindi) na antigen ya parasite.
Ikibazo.
Nigute dushobora gukemura ikibazo cyo kwihanganira ubudahangarwa bw'inyamaswa mugihe cyo gukingira indwara?

A.
Hindura immunogène usimbuza antigene z'umubiri hamwe na poroteyine za recombinant no gusimbuza virusi zirwanya umuriro na poroteyine zubaka za virusi. Hindura igishushanyo mbonera kandi uhindure dosiye yumubiri.
Ikibazo.
Nigute ubudahangarwa bw'umubiri bukingirwa muburyo bwa tekinoroji yo kwerekana?

A.
Ubudahangarwa bushobora kongerwa muguhuza antigene hamwe na poroteyine zitwara ibintu, nka bovine serum albumin. Byongeye kandi, imiti igabanya ubukana, harimo na Freund yunganira na aluminiyumu, irashobora gukoreshwa kugirango yongere igihe cyo gutura muri vivo kandi ishishikarize gukora no gukwirakwiza ingirabuzimafatizo z'umubiri, bityo bitezimbere ubudahangarwa bw'umubiri.

Ibibazo byo kubaka isomero ◢

Ikibazo.
Isomero rya antibody ya fage ni iki?

A.
Isomero rya antibody ya fage ni ikusanyirizo rya antibodi zitandukanye za fage zakozwe muguteranya urutonde rwimiterere yuturere duhindagurika duhereye kuri antibodi tujya mubice bya fage hanyuma tukabigaragaza hejuru yibice. Ubu buryo butuma habaho isomero rya fage antibody.
Ikibazo.
Inzira yo kubaka isomero rya antibody

A.
Inzira rusange yo kwerekana ibyiciro ikubiyemo intambwe zingenzi zingenzi: gukuramo ingirabuzimafatizo ya maraso monone nucléaire (PBMC) mu nyamaswa zakingiwe, gutandukanya RNA yose no kuyandukura muri ADN yuzuzanya (cDNA), kongera ingufu za gene ukoresheje tekinoroji ya polymerase (PCR), guhinduranya gene igenewe kuba selile ya fagitire. Ubu buryo butuma hubakwa isomero ryerekana fage, rishobora kumenya byihuse antibodiyite. Ibikurikira, iri somero rirashobora kuba isoko yingirakamaro mugutezimbere inkingo, imiti ivura, hamwe na reagent yo gusuzuma.
Ikibazo.
Ni izihe ngingo z'ingenzi zo kubaka isomero ryiza?

A.

Menya neza ko umubare rusange wa PBMC uhagije. Menya neza ubwiza bwa RNA nta gutesha agaciro.

Kugirango umenye umubare wimpinduka zose, ubushobozi bwibitabo burasabwa kuba inshuro 10 kugeza 100 zuburyo butandukanye.
Ikibazo.
Nibihe byiciro byububiko bwibitabo bwa fage antibody?

A.
Nk’uko inkomoko yububiko bwibitabo bwa antibody ibivuga, amasomero ya antibody ya fage arashobora gushyirwa mubitabo byibitabo byubudahangarwa bw'umubiri hamwe n'amasomero ya antibody ya fage. Itandukaniro ryibanze hagati yubwoko bubiri ni ukumenya niba inyamaswa zarakingiwe. Byongeye kandi, amasomero ya antibody kavukire arashobora kubyara binyuze muri kimwe cya kabiri cyangwa synthesis.
Ikibazo.
Nigute umuntu ashobora gusuzuma ubwiza bw'isomero rya fage antibody?

A.
Hariho ibice bibiri byibanze byo gusuzuma ubuziranenge bwibitabo bya antibody: ubushobozi bwo kubika no gutandukana. Ubushobozi bunini bwo kubika, bufatanije nubwinshi butandukanye, butuma igenzura ryiza rya antibodies zifitanye isano ryinshi kandi ryihariye.

Ibibazo byo Kugenzura Isomero ◢

Ikibazo.
Nubuhe buryo bwo gusuzuma isomero rya fage antibody?

A.
Muburyo bwo kuvanaho ibinyabuzima, isomero ryerekana ryashyizwemo intego zihamye hanyuma ryogejwe cyane kugirango rikureho ibyiciro bidakorwa. Conjugate isanzwe ivanwaho hakoreshejwe aside irike cyangwa umunyu mwinshi hanyuma ikongerwamo imbaraga mu ngirabuzimafatizo zibakira kugirango zikungahaze. Nyuma yo kuzenguruka 3 kugeza kuri 5, antibodies zifitanye isano ryinshi ziraboneka.
Ikibazo.
Ni ubuhe buryo bukoreshwa mu gusuzuma amasomero ya antibody ya fage?

A.
Uburyo bwo gusuzuma ibyiciro cyane cyane burimo ibice bikomeye byo gutekesha, kugenzura ibice byamazi, hamwe no kwerekana selile. Guhitamo uburyo bukwiye bwo gusuzuma bugomba gusuzuma ibiranga molekile igenewe, intego zokugenzura, hamwe na laboratoire.
Ikibazo.
Nibihe byiza kandi bibi byerekana ibice?

A.
Kugenzura neza nuburyo bwibanze bwo gusuzuma antibodiya ya fage ihuza poroteyine. Kugenzura nabi bifasha gukuraho antibodi zidafite akamaro. Mugihe cyo gusuzuma icyiciro cya antibody, antibodies zidafite akamaro zirashobora kumenyekana kubera kwivanga kwa label ya antigen, ibikoresho, cyangwa izindi antigene zifite imiterere isa na antigen. Mu bihe nk'ibi, gusuzuma nabi birashobora kongera imikorere yuburyo bwo gusuzuma.
Ikibazo.
Nigute wakwirinda gusuzuma antibodies zidafite akamaro?

A.

Antigens yanditseho ubwoko butandukanye bwibimenyetso irashobora gushirwa imbere mugupima kwambere, cyangwa kwipimisha nabi birashobora gukoreshwa kugirango igabanye igipimo cya antibodi zidafite akamaro.

Urashobora guhitamo gusimbuza amazi akwiye cyangwa ubundi buryo bwo gukoresha amazi atandukanye kugirango wirinde iki kibazo. Niba antibody imenyekanisha umurongo winyuma (CHO cyangwa HEK293) irerekanwa, urashobora gusimbuza imirongo itandukanye ya selile yo kugenzura cyangwa kugenzura nabi.
Ikibazo.
Nigute umuntu ashobora kugenzura no gusesengura antibodi zabonetse?

A.
Uburyo bwo kugenzura antibody mubisanzwe harimo ELISA, Western Blotting (WB), flux cytometrie, immunoprecipitation (IP), immunofluorescence (IF), immunohistochemie, nubundi buryo butandukanye bwubushakashatsi. Alpha Lifetech ifite uburambe bunini mugushakisha antibody kandi ifite itsinda ryabashakashatsi babigize umwuga, ryemeza imikorere ya antibodies mubushakashatsi bwakurikiyeho.

Niba ufite ikibazo, nyamuneka twandikire igihe icyo aricyo cyose.

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