Urubuga rwo guteza imbere ikoreshwa ry'abarwanya umubiri

Ubudahangarwa bw’umubiri bw’umuntu butera ingaruka nke cyangwa nta n’imwe butera ubudahangarwa bw’umubiri bw’umuntu. Dukurikije ubushakashatsi ku isoko ry’ubushakashatsi ku isoko, isoko ry’isi ry’ubudahangarwa bw’umubiri bw’abantu ni miliyoni 92.5 z’amadolari y’Amerika mu 2024 kandi rizakura ku gipimo cy’izamuka ry’umubiri ku mwaka (CAGR) cya 13.00% kuva mu 2024 kugeza mu 2031. Alpha Lifetech ifite urubuga rwo gukwirakwiza ubudahangarwa bw’umubiri bw’abantu, rufite igipimo cyo gutsinda kirenga 98% ku budahangarwa bw’umubiri bw’abantu. Dufite ingamba nyinshi zo guhindura ubudahangarwa bw’umubiri, harimo gushyira CDR mu mubiri, gushyira SDR mu mubiri, guhuza urunigi, kwerekana phage, nibindi, bishobora gutoranywa hakurikijwe ibyo ukeneye mu igerageza. Ntidushobora gusa gutanga ubudahangarwa bw’umubiri bw’ubwoko butandukanye, nk’imbeba, inkwavu, alpaca, ingamiya, nibindi, ahubwo dushobora no guhindura ubudahangarwa bw’umubiri mu buryo butandukanye, nka scFv, Fab, na nanobodies. Dufite abakozi b’inzobere mu bya tekiniki batanga serivisi zo gukora, gutunganya no kwemeza ubudahangarwa bw’umubiri, harimo gukora ubudahangarwa bw’umubiri bw’imbeba, gukora ubudahangarwa bw’umubiri bw’abantu, kugaragaza no gutunganya ubudahangarwa bw’umubiri bw’abantu, no gusobanura no gusesengura ubudahangarwa bw’umubiri bw’abantu. Dufite igipimo cyo gutsinda cyane, ubuziranenge buhanitse, kandi dufite ubushobozi buke bwo kurwanya indwara, twemeza ko hakorwa antibody ikoresheje imiterere y’umubiri.

Intangiriro yo guhindura umubiri w’umuntu urwanya indwara

Uburyo bwo kuvura indwara ziterwa n’umubiri zirwanya kanseri bushobora gukoreshwa mu kuvura indwara ziterwa n’umubiri, kanseri, n’izindi ndwara. Ubudahangarwa bwa monoclonal bukorwa binyuze mu gukingira imbeba cyangwa izindi nyamaswa. Iyo bukoreshejwe mu kuvura indwara mu cyiciro cya nyuma cyo gukora ubudahangarwa bwa monoclonal, ubushobozi bw’ubudahangarwa bw’umubiri buterwa n’umubiri butari ubw’umuntu ntibushobora kwirengagizwa. Ihame nyamukuru ryo guhindura imiterere y’umubiri ni ugushyira ibisigazwa by’umubiri bitari ubw’umuntu, nk’uturere dupima ubudahangarwa buhindagurika cyane (CDRs), mu buryo bw’umubiri w’umuntu, bigatanga uruhererekane rugumana imiterere y’umubiri w’umwimerere mu gihe hirindwa ubudahangarwa bw’umubiri. Gukingira imbeba no guhindura imiterere y’umubiri w’imbeba nyuma yaho biracyari inzira ebyiri z’ingenzi zo kuvumbura ubudahangarwa bw’umubiri buvura, ahanini bunyura mu byiciro byinshi: gukora ubudahangarwa bw’umubiri bw’imbeba, gukuraho ubudahangarwa bw’umubiri bw’umuntu bukomoka kuri monoclonal, no guteza imbere ubudahangarwa bw’umubiri bw’umuntu bukomoka kuri monoclonal bwuzuye. Muri iki gihe, ubushakashatsi bugizwe ahanini n'imbeba zahinduwe mu jini (kwimura jeni za B mu mbeba), gusuzuma umusaruro mwinshi hakoreshejwe ikoranabuhanga ryo kwerekana umusemburo cyangwa phage, gushyira CDR mu byiciro (gushyira CDR mu byiciro by'abantu), gupima ibisigazwa by'ingirabuzimafatizo (SDR), guhuza imiterere y'umubiri, n'ubundi buryo.

gukora inyito y'umubiri (antibody humanisation) - Alpha Lifetech

Igishushanyo cya 1: Incamake y'uburyo antibody ihinduka umuntu kuva kuri antibody zo mu bwoko bwa murine (ahantu heza ho mu bwoko bwa green) kugeza kuri antibody zuzuye z'abantu.

Ingamba zo guhindura umubiri urwanya indwara

Gushyira CDR ku murongo

Gushyira CDR mu mubiri bikorwa binyuze mu buryo bwo kugaragaza imiterere y’inyamabere bushingiye ku ikoranabuhanga rya ADN rihuza, kandi intambwe zaryo z'ingenzi ni izi zikurikira:

(1) Kora antibody zijyanye nazo mu mbeba (cyangwa izitari iz'abantu), tandukanya antibody zikora ADN, hanyuma uzishyire mu buryo bwo kuzikurikirana (cyangwa uzikore mu buryo butaziguye mu buryo bw'ingirabuzima fatizo imwe).

(2) Kumenya urukurikirane rwa ADN rujyanye na CDR y’umubiri w’umuntu no kumenya umwihariko w’aho intego ihurira;

(3) Hitamo agace k'imiterere y'umubiri w'umuntu (FR) kugira ngo uteremo CDR zitari iz'umuntu hanyuma ukore gene nshya y'umubiri w'umuntu.

(4) Kora isesengura ry’impande eshatu ry’amakimbirane ari hagati ya CDR zitari iz’abantu na FR z’abantu kugira ngo habeho impinduka zo kugarura ubuzima kugira ngo habeho ituze kandi hakumirwe gutakaza isano cyangwa ubusugire bw’imiterere y’umubiri wa nyuma w’umubiri w’umuntu.

Ishusho ya 2: Incamake y'icyitegererezo cy'uburyo bwo guhuza akarere (CDR) bwo guteramo imbuto.

Guhuza umurongo

Guhuza imiterere y’urusobe rw’amakuru bishingiye ku ikoranabuhanga ryo kwerekana phage n’ikoranabuhanga ryo gusuzuma isomero rya phage kugira ngo haboneke antibodies zifatanye cyane. Oligonucleotides zikoresha uturemangingo tw’uruhererekane rw’amakuru aremereye akoreshwa nk’ibishushanyo, uturere twa CDR dukoreshwa nk’inyuguti za primers, kandi PCR amplification ikorwa kugira ngo hakorwe PCR product kugira ngo haboneke DNA ifite umurongo umwe. DNA ifite umurongo umwe ya VL na VH genes isukurwa na streptavidin ishyirwa ku turemangingo hakoreshejwe T4 DNA ligase na restriction enzymes, ishyirwamo bacteriophages, kandi ikongerwamo bacteriophages, hanyuma isomero rya phage rikubakwa. Hakorwa isuzuma ritatu hakoreshejwe antigens zijyanye naryo, kandi clones nziza zigenzurwa burundu hakoreshejwe ELISA n’ubundi buryo.

guhindura umubiri w’umuntu urwanya indwara

Ishusho ya 3: Ishusho y'icyitegererezo cy'ingamba zo guhuza akarere (CDR) n'uburyo bwo guhuza imiterere y'ubutaka (FR). (A) Uburyo bw'ingenzi bwo guhuza imiterere y'ubutaka (CDR). (B) Uburyo bw'ingenzi bwo guhuza imiterere y'ubutaka (FR). (Inkomoko y'aho: Wang Yongmei, n'abandi, Kugereranya "guhuza imiterere" na "gushyira CDR mu mubiri" mu guhindura umubiri w'umuntu antibody ya PD-1 murine.)

Icyerekezo cya Phage

Gukora antibodies zakozwe n’abantu bishingiye ku buryo bwo kwerekana antibodies zihariye ku buso bwa bacteriophages, ahanini zubakwa binyuze mu bubiko bwa antibodies za phage z’abantu. Inkomoko y’ubu bubiko ni PBMCs zakuwe mu maraso y’umuntu kugira ngo zubakwe ibitabo bya phage, aho ibice bihuye na byo bisuzumwa. Ubu buryo bufite urutonde rwuzuye rw’abantu, ariko antibodies zakozwe ni ibice bya antibodies, nka scFv, Fab, na nanobody.

Imikorere ya serivisi yo gukwirakwiza abarwayi ba antibody

Intambwe za serivisi	Ibipimo ngenderwaho bya QC	Ingengabihe
Kwitandukanya kw'abarwayi n'umubiri	Ubwiza >95%	Icyumweru 1-2
Isesengura ry'uruhererekane	Kumenya neza uruhererekane rw'ibintu bitari iby'abantu	Icyumweru kimwe
Gushyira CDR mu byiciro no Gushyira mu byiciro	Kwishyira hamwe no kugaragaza ibitekerezo neza	Ibyumweru 2-3
Igaragaza n'Isukura	Umusaruro mwinshi n'ubuziranenge	Ibyumweru 2-3
Kwemeza imikorere	Isuzuma ry'isano n'iry'umwihariko	Icyumweru kimwe

Ibyiza byo gukoresha uburyo bwo kurwanya indwara mu mubiri (Antibody Humanization Platform)

Alpha Lifetech ifite urubuga rwo gukwirakwiza uturemangingo tw’umubiri tw’abantu bakuze, rufite igipimo cyo gutsinda kirenga 98% ku turemangingo tw’umubiri tw’abantu.