Serivisi yo gusesengura imiterere ya Aptamer

Alpha Lifetech imaze imyaka myinshi itanga serivisi zijyanye na aptamer, kandi ubu ishobora gutanga gahunda zuzuye zo guteza imbere aptamer nko guhuza aptamer, gusuzuma SELEX, gukurikirana ibintu byinshi, no kunoza no gusobanura aptamer. Hashingiwe ku byiza by'uburyo bwa SELEX bwo gusuzuma aptamers, Alpha Lifetech yongereye uburyo bwinshi bwo gusuzuma aptamers.

Intangiriro ku isesengura ry'imiterere ya Aptamer

Igenzura ry'ubumwe

Ubufatanye bwa aptamer bwakozwe neza bwemejwe hakoreshejwe ubuhanga bujyanye n'ikizamini cyo gufata aptamer. Ingero zirimo Isothermal Titration Calorimetry (ITC), Flow Cytometry (FCM), na Surface Plasmon Resonance (SPR), Microfluidics, nibindi. Ubusanzwe ubufatanye bwa aptamer bugaragazwa na dissociation constant (KD), ingano ifatika ikoreshwa mu gupima urwego rw'itandukaniro rya molekile mu buryo bushobora guhinduka. Uko agaciro ka KD kaba gato, niko complex iba ihamye, ni ukuvuga ko ubufatanye bukomera; Ibinyuranye n'ibyo, uko agaciro ka KD kaba kanini, niko complex iba idafite ishingiro kandi ubufatanye bugakomera. Iyi ntambwe ni ingenzi mu kumenya imikorere ya aptamer, kwemeza ko attamer idafite ishingiro ikura kandi ishobora kwihuza na molekile y'ingenzi ifite ubufatanye bwinshi n'uburyo bwo guhitamo mu bikorwa bifatika.

Igishushanyo cya 1. Inzira yo gukura kwa aptamer affinity. Inkomoko y'aho iboneka:Kinghorn AB, Fraser LA, 2017.

Igenzura ry'imikorere

Uretse kugenzura affinity, imikorere ya aptamer igomba kwemezwa. Ibi birimo kugenzura ihamye ya aptamer, umwihariko wayo, n'imikoranire yayo n'izindi molekile mu gace runaka. Ibisubizo by'igenzura ry'imikorere bizagira ingaruka zitaziguye ku gaciro ka aptamers mu bushakashatsi bwa siyansi no mu nganda.

Igenzura ryihariye

Igerageza ry’irushanwa: Ubushobozi bwa aptamer bwo gufatana n’ikintu runaka busuzumwa imbere y’ibindi bintu bisa. Niba aptamer ishobora gufatana by’umwihariko n’ikintu runaka nta mpungenge z’izindi molekile, bigaragaza ko ifite umwihariko wo hejuru.

Igerageza ry’uburyo bwo gukora ibintu bitandukanye: Aptamer ihuzwa n’uruhererekane rw’ibipimo bifitanye isano cyangwa bidafite aho bihuriye kugira ngo harebwe niba ihuzwa gusa n’ikintu runaka, kugira ngo harebwe neza niba ari ikintu cyihariye.

Igenzura ry'ubudahinduka

Igerageza ryo kwangirika kwa nuclease: Aptamers zahuye n’ibipimo bitandukanye bya nucleases kandi kwangirika kwazo kwagaragaye. Mu kugereranya urwego rwo kwangirika mu bihe bitandukanye, ubushobozi bwa aptamer bwo kurwanya kwangirika kwa nuclease bushobora gusuzumwa.

Igerageza ryo kugabanya ubushyuhe n'igihe: Aptamer yashyizwe mu bushyuhe n'igihe bitandukanye kugira ngo irebe ko imiterere yayo n'imikorere yayo bihamye. Ibi bifasha kumenya uburyo bwiza bwo kubika no gukoresha aptamer.

Kumenya ibikorwa by'ibinyabuzima

Uburyo bukwiye bukunze gutoranywa hakurikijwe intego yihariye y'ikoreshwa rya aside nucleic aptamer.

(1) Gupima urwego rw'ingirabuzimafatizo: Gukoresha tekiniki za biology ya molekile, nka gel electrophoresis, Western blot, nibindi, kugira ngo hamenyekane imiterere yakozwe nyuma y'uko aptamer ihujwe na molekile y'ingenzi, cyangwa hamenyekane impinduka mu rwego rw'imvugo ya molekile y'ingenzi iterwa na aptamer.

(2) Gupima urwego rw'uturemangingo: Hakoreshejwe ikoranabuhanga ryo kwita ku turemangingo, uturemangingo dushyirwa mu turemangingo kugira ngo harebwe impinduka mu binyabuzima nko mu miterere y'uturemangingo, kwiyongera no kugabanuka kw'uturemangingo, no gusuzuma imikorere y'uturemangingo.

(3) Isuzuma ry’ingero z’inyamaswa: Mu matsinda y’inyamaswa akwiye, aptameri za aside nucleic zitangwa hakoreshejwe inshinge cyangwa imiti, kandi ibimenyetso by’imiterere y’inyamaswa n’impinduka mu mikorere y’inyamaswa birebwa kugira ngo harebwe imikorere y’ibinyabuzima n’umutekano wa aptameri mu mubiri.