Iterambere rya Aptamer

Aptamers ni oligonucleotide imwe (ADN, RNA cyangwa XNA) ifite imitungo myinshi kandi yihariye ihuza cyane na molekile yibasira nka antibodi, kandi ikoreshwa cyane mugutezimbere biosensor, gusuzuma no kuvura.

Ihuriro rya aptamer ryatanzwe na Alpha Lifetech ririmo ibyiciro bibiri: urubuga rwa aptamer synthesis, rurimo cyane cyane serivise ya synthesis ya SELEX aptamer hamwe na serivise yiterambere rya aptamer (ADN, RNA cyangwa XNA), hamwe na porogaramu yo gusuzuma aptamer harimo serivisi zo gusuzuma zishingiye ku ikoranabuhanga rya SELEX kuri poroteyine, peptide, selile, molekile ntoya, hamwe na molekile zipima neza, ndetse no hasi ya molekile,

Ihuriro rya Aptamer

SELEX aptamer isomero rya serivise

Serivise ya SELEX aptamer isobanura cyane cyane mukubaka isomero ririmo umubare munini wurutonde rwa oligonucleotide rukurikiranye hamwe na vitro chimique synthesis ukurikije molekile zigenewe. Kubaka isomero nintangiriro yubuhanga bwa SELEX, butanga urutonde rwabakandida benshi murwego rwo gukurikiranwa nyuma yubaka amasomero manini atunguranye kandi byongerera amahirwe yo gusuzuma aptamers nyinshi.

Isomero ryibitabo rigabanijwemo intambwe zikurikira:

Intambwe	Ibisobanuro by'ikoranabuhanga
Menya intego ya molekile	Menya intego ya molekile ikeneye gusuzumwa kuri aptamers, ishobora kuba proteyine, acide nucleic, molekile nto, ion ibyuma, nibindi.
Igishushanyo gikurikiranye	Uburebure bukurikiranye, ibice fatizo nibindi bipimo byakozwe ukurikije ibiranga molekile igenewe nibisabwa byo gusuzuma. Mubisanzwe, urutonde rudasanzwe ruri hagati ya mirongo na magana shingiro muburebure.
Synthesis yuburyo bukurikiranye	Ibice bya Oligonucleotide hamwe nibihe byagenwe (nka PCR primer ikurikirana) kumpande zombi byateguwe kandi bigahuzwa, bizakoreshwa mugihe cyo gukuza no gusuzuma.

Isomero ryisomero riracyakeneye gutunganywa kugirango igenzurwe ubuziranenge. Ubwinshi bw'isomero bwariyemeje kwemeza ko bukurikizwa mugikorwa cyakurikiyeho. Itandukaniro nukuri kwuruhererekane rwateganijwe mubitabo byagenzuwe nuburyo bukurikirana hamwe nubundi buryo kugirango ireme ryibitabo ryujuje ibisabwa byo gusuzuma.

Binyuze mu ntambwe zavuzwe haruguru, isomero ryo mu rwego rwohejuru kandi ritandukanye cyane rya SELEX aptamer isomero rishobora guhuzwa, rishobora gutanga urutonde rwabakandida benshi kugirango bakurikirane ibizakurikiraho.

Serivisi ziterambere rya Aptamer (ADN, RNA cyangwa XNA)

Aptamers mubisanzwe yerekeza kuri acide nucleic aptamers. Nucleic acide aptamers yinjiza ADN aptamers, RNA aptamers, na XNA aptamers ihindurwamo imiti aptamers acide nucleic. Tekinike ya SELEX ikoreshwa cyane mugutezimbere aptamers. Ibikorwa byibanze bya serivisi ziterambere rya aptamer zirimo kubaka isomero, guhuza intego, kwigunga no kwezwa, amplification, ibyiciro byinshi byo gusuzuma, no kumenya urutonde. Kumyaka myinshi, twibanze ku kubaka amasomero nuburambe bukomeye mugutezimbere aptamer. Twiyemeje guha abakiriya serivisi nziza.

Uburyo bwa tekinoroji ya SELEX

Inzira ya SELEX igizwe nuruziga rwinshi, buri kimwe kigizwe nintambwe zingenzi zikurikira:

Isomero hamwe no Guhuza Intego

Isomero ryubatswe rya acide nucleique rivanze na molekile yihariye (nka proteyine, ibice bito bya molekile, nibindi), kugirango acide nucleic ikurikirana mubitabo ifite amahirwe yo guhuza na molekile yagenewe.

Kwigunga kwa molekile zidafunze

Urutonde rwa acide nucleique rudahujwe na molekile yintego rutandukanijwe nuruvange nuburyo bwihariye nka affinity chromatography, gutandukanya amasaro ya magneti, nibindi.

Kongera imbaraga za molekile

Urutonde rwa acide nucleic ihujwe na molekile yintego irongerwa, mubisanzwe ukoresheje tekinoroji ya polymerase (PCR). Kubyiciro bizakurikiraho, ibyiciro byongeweho bizakoreshwa nkububiko bwibitabo.

Igishushanyo 1: Igikorwa cyo gusuzuma SELEX

Aptamer Yerekana

Serivisi yo gusuzuma Aptamer

Alpha Lifetech itanga urwego rutandukanye rwa serivisi yihariye yo gusuzuma aptamer ikoresha uburyo butandukanye bwa SELEX kuburyo butandukanye bwa molekile yawe:

Ubwoko bw'Intego	Ibisobanuro bya tekiniki
Poroteyine Aptamer Yerekanwa na SELEX	Intego nyamukuru yo gusuzuma protein aptamer ni ukugaragaza aptamers ishobora guhuza cyane na molekile ya poroteyine. Izi aptamers ziroroshye guhuza, zihamye kandi ntizishobora kwibasirwa nibidukikije.
Peptide Aptamer Yerekanwa na SELEX	Peptide aptamers nicyiciro cya peptide ngufi ikurikiranye cyane kandi yihariye, irashobora guhuza cyane cyane nibintu bigamije kandi ikerekana uburyo bwinshi bwo gukoresha mubinyabuzima. Binyuze mu buryo bwihariye bwo gusuzuma, peptide aptamers ishobora guhuza cyane na molekile igenewe isuzumwa uhereye ku mubare munini w'amasomero akurikirana ya peptide.
Kugenzura Aptamer Yihariye (Cell-SELEX)	Intego za selile cyangwa molekile zihariye hejuru yakagari zateguwe nkintego. Intego zirashobora kuba selile zose, reseptors kuri selile selile, proteyine, cyangwa izindi molekile nto.
Gutoya ya Molecule Aptamer Yerekanwa na Capture SELEX	Gufata SELEX ni tekinike yo gusuzuma vitro yo gusuzuma molekile ntoya ya aptamers, ikaba ari variant ya SELEX. Gufata SELEX irakwiriye cyane cyane mugusuzuma aptamer yerekana intego ntoya ya molekile, ubusanzwe ifite amatsinda make yimikorere kandi biragoye kwimuka muburyo butaziguye.
Serivisi nzima zishingiye kuri SELEX	Serivise nzima ishingiye ku nyamaswa ni tekinike yubushakashatsi ikoreshwa cyane mubijyanye na biyogi, ubuvuzi na biotechnologiya, ikoresha inyamaswa nzima nkicyitegererezo cyubushakashatsi kugirango isuzume kandi isuzume molekile yihariye, imiti, imiti cyangwa inzira y'ibinyabuzima. Serivisi zagenewe kwigana ibidukikije byumubiri mumubiri wumuntu kugirango tumenye neza kandi dusuzume neza numutekano wibisubizo byubushakashatsi mumubiri wumuntu.

Serivisi nziza ya Aptamer

Hydrophilicity, gutakaza umubyibuho ukabije mugihe cyo kubyara, no gusohora byihuse aptamers bigabanya kubishyira mubikorwa. Kugeza ubu, uburyo butandukanye bwo gutezimbere bwashakishijwe kugirango tunoze imikorere ya aptamers.

Dufite kandi uburyo butandukanye bwo guhindura aptamer igizwe no guhagarika, guhindura, guhuza itsinda ryabigenewe (thiol, carboxy, amine, fluorophore, nibindi).

Serivisi yo gusesengura ibiranga Aptamer

Serivise yo gusesengura ibiranga Aptamer bivuga serivisi yumwuga yo gusuzuma imikorere yimikorere no kugenzura imikorere ya aptamer wabonye kugirango aptamer yujuje ubushobozi bwihariye bwo guhuza, gutuza nibisabwa byihariye. Harimo cyane cyane isesengura ryihariye hamwe nisesengura ryihariye, gusuzuma ituze no kugenzura imikorere yibinyabuzima.