Serivisi yo gukoresha ibikoresho bya Aptamer

Aptamers ni urunigi rumwe oligonucleotide ishobora guhuza byumwihariko kuri molekile. Bakora imiterere-yuburyo butatu nyuma yo guhuza n'imihindagurikire y'ikirere binyuze mu mbaraga zinyuranye zikorana nka nucleotide base yuzuzanya, guhuza hydrogène, π-π guteranya, ingufu za electrostatike, nibindi. Iyi miterere ihuza cyane cyane na molekile ikoresheje imbaraga za intermolecular. Aptamers isanzwe ikorwa na SELEX yerekana. Uburyo bwiza bwa Aptamer bwibanda cyane cyane kunoza ubumwe, guhitamo no gutuza kwa aptamers.

Alpha Lifetech yiyemeje guha abakiriya serivisi nziza kandi nziza ya nucleic aside aptamer yo gusuzuma no gutanga serivisi nziza. Kugenzura no gutezimbere ntabwo ari intambwe yingenzi yo kubona aptamers yo mu rwego rwo hejuru, ahubwo ni umusingi wingenzi wo guteza imbere izo porogaramu. Alpha Lifetech ikomeje kumenyekanisha ikoranabuhanga rigezweho no kunoza imikorere ya serivisi kugirango tumenye neza ko serivisi zacu zihora ku isonga mu nganda kandi zikazana abakiriya kurushaho gukora neza nucleic aside aptamer gusuzuma no kunoza serivisi nziza.

SELEX Guhitamo Aptamer

Alpha Lifetech imaze imyaka myinshi yibanda ku iterambere rya acide nucleic acide aptamer, cyane cyane igabanijwemo igishushanyo mbonera cya acide nucleic acide, kubaka isomero rya nucleic acide aptamer, isuzuma rya acide nucleic aptamer hamwe na synthesis ya nucleic acide aptamer.

Tekinike yo gusuzuma SELEX nuburyo nyamukuru bwo gusuzuma aptamers. Alpha Lifetech ntishobora gutanga gusa guhitamo aptamer ya SELEX, ahubwo irashobora no gutanga ubundi buryo bushingiye ku ikoranabuhanga rya SELEX, nka Cell-SELEX, CE-SELEX, Capture-SELEX, nibindi. Binyuze muri vitro yo gusuzuma aptamers, aptamers ishobora kumenya neza molekile yintego kandi ikaba ifitanye isano ryinshi na molekile yintego irabonetse, hanyuma hakurikiraho gukurikiranwa cyane bikorerwa kuri aptamers yerekanwe, hanyuma amaherezo aptamers ikomatanyirizwa muri vitamine ya vitamine. Mubyongeyeho, Alpha Lifetech irashobora guhitamo aptamers yatoranijwe no kugenzura imikorere ya aptamers nziza.

Intangiriro kuri Aptamer Optimisation

Nubwo aptamers acide nucleic yerekanwa nubuhanga bwa SELEX ifitanye isano ikomeye na molekile yintego, mubikorwa bifatika, aptamers iracyakeneye kunonosorwa kugirango irusheho kunoza umwihariko wa aptamer, isano ya aptamer hamwe na aptamer ihamye kugirango ihuze ibyifuzo byihariye.

Uburyo nyamukuru bwo gukoresha aptamer burimo ibintu bikurikira:

Gukata imiterere

Gucamo ibice: Mugukuraho ibice byurwego rwa aptamer bitagira ingaruka cyangwa bike bigira ingaruka kubushobozi bwo guhuza molekile yintego, kubungabunga ahantu hamenyekanye, uburebure bwa aptamer burashobora kugabanuka, kandi imikorere ya synthesis hamwe na aptamer ihagaze neza.

Hashingiwe ku miterere ya kabiri yo kwigana no kugerageza no kwibeshya, imiterere yo hejuru ya aptamer (nk'impeta y'uruti, pseudojunction, G-quadruplet, nibindi) yasobanuwe muburyo bwa artificiel, iminyururu imwe idahinduwe cyangwa ikomatanyirijwe hamwe, uruti ruragabanuka, impeta ntoya ya convex yakuweho, impeta ziragabanuka, nibindi.

Kumenyekanisha ihinduka

Guhinduranya bisanzwe. Ubu buryo bushobora gucukumbura itandukaniro rya aptamer no kuvumbura uburyo bushya bwo guhuza hamwe nicyerekezo cyiza.

Urubuga rwihariye: Dushingiye ku isesengura ryimiterere no guhuza ibibanza byahanuwe, imbuga zishoboka za mutation zatoranijwe kumurongo umwe cyangwa gusimbuza byinshi-kugirango bashakishe ingaruka zurutonde rutandukanye kumikorere ya aptamer. Urubuga rwihariye rwa mutation irashobora kugenzura neza impinduka zimiterere ya aptamer kugirango ihindure imikorere yayo.

Guhindura imiti

Aptamer yahinduwe muburyo bwa chimique, kandi amatsinda yo guhindura atangizwa mumyanya itandukanye ya aptamer (nkibishingwe, impeta yisukari, itsinda rya fosifate, nibindi) kugirango bitezimbere, ubushobozi bwo kurwanya nuclease hamwe nubusabane bwa aptamer.

Igishushanyo.1 Guhindura bisanzwe bya aptamers. (Inkomoko yerekana:Nucleic-aside "Igitabo," Nucleic aside aptamers "Umutwe

Ihinduka risanzwe ririmo 2 '-fluororibose, 2' -amino ribose, 2 '-o-methylribose, LNA (acide nucleic aside ifunze), hamwe na aside nucleic acide itavanze (UNA). Ihinduka rishobora kugabanya kumenyekanisha nuclease, kongera ubushobozi bwa aptamers yo kurwanya iyangirika, cyangwa guhindura imitungo yabo ihuza molekile.

Kumenyekanisha Agace Agace

Aptamer yahawe imirimo mishya, nkibikorwa bya enzyme nubushobozi bwo gutanga raporo ya fluorescence. Agace ka ligand-gahuza kahujwe nakarere ka catalitiki kugirango bitange aptamer hamwe nibikorwa bya enzyme. Kurugero, kongeramo uturere duhuza na molekile yohereza fluorescence itanga raporo yo kwiyitirira aptamer itanga ibimenyetso bya fluorescent nyuma yo guhuza intego neza.