Serivisi yo kunoza Aptamer

Aptamers ni oligonucleotides zo mu muyoboro umwe zishobora kwihambira ku buryo bwihariye kuri molekile zigamije. Zikora imiterere yihariye y’ibice bitatu nyuma yo kwihuza binyuze mu mbaraga zitandukanye z’imikoranire nko kwihuza kw’ibanze bya nucleotide, guhuza hydrogen, gukusanya π-π, imbaraga za electrostatic, nibindi. Iyi miterere ihambira ku buryo bwihariye kuri molekile zigamije kwihambira binyuze mu mbaraga za molekile. Aptamers ubusanzwe zikorwa hakoreshejwe uburyo bwa SELEX. Uburyo bwo kunoza Aptamers bwibanda cyane cyane ku kunoza uburyo bwo guhuza, guhitamo no guhagarara neza kwa aptamers.

Alpha Lifetech yiyemeje guha abakiriya serivisi zo gusuzuma no kunoza aside nucleic aptamer neza kandi neza. Uburyo bwo gusuzuma no kunoza aside nucleic aptamer si intambwe y'ingenzi gusa mu kubona aptamer nziza, ahubwo ni n'ishingiro ry'ingenzi mu guteza imbere izi porogaramu. Alpha Lifetech ikomeje gushyiraho ikoranabuhanga rigezweho no kunoza inzira za serivisi kugira ngo serivisi zacu zihore ziri ku isonga mu nganda kandi zihe abakiriya ubunararibonye bwiza bwo gusuzuma no kunoza aside nucleic aptamer.

Guhitamo SELEX Aptamer

Alpha Lifetech imaze imyaka myinshi yibanda ku iterambere rya aside nucleic aptamer, ahanini bigabanyijemo igishushanyo mbonera cya aside nucleic aptamer, imiterere y’isomero rya aside nucleic aptamer, gusuzuma aside nucleic aptamer na synthesis ya aside nucleic aptamer.

Uburyo bwo gusuzuma SELEX ni bwo buryo bw'ingenzi bwo gusuzuma aptamer. Alpha Lifetech ntabwo ishobora gusa gutanga uburyo bwo guhitamo aptamer ya SELEX, ahubwo ishobora no gutanga ubundi buryo bushingiye ku ikoranabuhanga rya SELEX, nka Cell-SELEX, CE-SELEX, Capture-SELEX, nibindi. Binyuze mu gusuzuma aptamer muri vitro, aptamer zishobora kumenya neza molekile y’ingenzi kandi zifite isano iri hejuru na molekile y’ingenzi, hanyuma hagakorwa sequencing ya high-throughput kuri aptamer zasuzumwe, hanyuma aptamer zigakorwa binyuze mu guhuza ibinyabutabire muri vitro. Byongeye kandi, Alpha Lifetech ishobora kunoza aptamer zatoranijwe no kugenzura imikorere ya aptamer zasuzumwe.

Intangiriro yo gukoresha Aptamer Optimization

Nubwo aptameri za aside nucleic zasuzumwe n'ikoranabuhanga rya SELEX zifite ubufatanye bukomeye kuri molekile zigamije, mu bikorwa bifatika, aptameri ziracyakeneye kunozwa kugira ngo zirusheho kunoza imiterere ya aptameri, uburinganire bwa aptamer n'ubudahangarwa bwa aptamer kugira ngo bihuze n'ibikenewe mu buryo bwihariye bwo gukoreshwa.

Uburyo bw'ingenzi bwo kunoza aptamer burimo ibi bikurikira:

Gukata imiterere

Gukata: Mu gukuraho ibice by'uruhererekane rwa aptamer bitagize ingaruka cyangwa ngo bigire ingaruka ku bushobozi bwo gufatanya bwa molekile y'ingenzi, bikarinda agace k'ibanze k'igenzura, uburebure bwa aptamer bushobora kugabanywa, kandi imikorere yayo mu gukora no kudahinduka kwa aptamer bishobora kunozwa.

Hashingiwe ku miterere y’inyongera hamwe n’igerageza n’amakosa, imiterere yo hejuru ya aptamer (nk’impeta y’umugongo, pseudojunction, G-quadruplet, nibindi) yagenwe mu buryo bw’ubukorano, iminyururu imwe idakozwe cyangwa ifatanye yarakuweho, uruti ruragabanuka, impeta nto zipfundikiye zirakurwaho, impeta zagabanutse, nibindi. Ikoranabuhanga ryo gufunga molekile rikoreshwa mu guhanura imiterere y’ibice bitatu bya aptamer na molekile y’icyerekezo kugira ngo hakorwe neza.

Tuzana impinduka mu mikorere y'umubiri

Impinduka mu buryo butunguranye: Gukoresha tekiniki za mutagenesis (nka PCR mutagenesis) kugira ngo hamenyekane impinduka mu buryo butunguranye mu gice cya aptamer, no kongera gusuzuma hakoreshejwe ikoranabuhanga rya SELEX bishobora kubona impinduka zifite ubwiyongere n'uburyo bwo guhitamo. Ubu buryo bushobora gusuzuma ubudasa bw'ibice bya aptamer no kuvumbura imiterere mishya yo guhuza hamwe n'icyerekezo cyo kunoza.

Impinduka mu gace runaka: Hashingiwe ku isesengura ry’imiterere n’iteganyagihe ry’aho hantu hahurira, ahantu hashobora guhinduka hatoranywa kugira ngo hahindurwe ahantu hamwe cyangwa ahantu hanini kugira ngo harebwe ingaruka z’uruhererekane rutandukanye ku mikorere ya aptamer. Ihinduka ry’ahantu runaka rishobora kugenzura neza impinduka z’imiterere ya aptamer kugira ngo inoze imikorere yayo.

Guhindura imiti

Aptamer ihindurwa hakoreshejwe imiti, kandi amatsinda yo guhindura ashyirwa mu myanya itandukanye ya aptamer (nk'ishingiro, isukari, itsinda rya fosifate, nibindi) kugira ngo yongere ubushobozi bwo kudahinduka, kurwanya nuclease ndetse n'ubudahangarwa bwa aptamer.

Igishushanyo cya 1 Impinduka zisanzwe za aptamers. (Inkomoko y'inyongera:Igitabo cya aside nucleic", Igice cya "Aptamers za aside nucleic"

Impinduka zisanzwe zirimo 2 '-fluoribose, 2' -amino ribose, 2 '-o-methylribose, LNA (aside nucleic locked), na aside nucleic purine (UNA) idakozwe muri methyl). Izi mpinduka zishobora kugabanya kumenyekana kwa nucleases, kongera ubushobozi bwa aptamers bwo kurwanya kwangirika, cyangwa guhindura imiterere yazo yo gufatana na molekile zigamije.

Akarere k'Imikorere katangiza

Aptamer ifite imikorere mishya, nko gukora enzymes no gutanga raporo y'urumuri. Agace gahuza ligand gahuzwa n'agace ka catalytic kugira ngo hakorwe aptamer ifite enzymes. Urugero, kongeramo uduce duhuza na molekile zisohora fluorescence birema aptamer yitanga itanga amakuru ubwayo itanga ikimenyetso cy'urumuri nyuma yo gukora neza.