Serivisi ishinzwe ubushakashatsi

Intangiriro kuri Aptamer

Aptamers ni acide nucleic acide imwe (nka ADN cyangwa RNA) molekules zerekanwa muri vitro zivuye mumasomero ya oligonucleotide ya syntetique hamwe nuburyo bwateganijwe kubushake bwiswe SELEX, bikubiyemo ibyiciro byinshi byo gutoranya itera. Ibice byingenzi bigize serivisi ziterambere rya aptamer zifitwe na Alpha Lifetech zirimo kubaka isomero rya aptamer, kwerekana aptamer SELEX, kwerekana aptamer SELEX, isesengura rya aptamer hamwe nisesengura rya aptamer.

Urutonde rwa SELEX rukomatanya aptamer SELEX yerekana hamwe nibisohoka byinshi. Binyuze kuri aptamer SELEX ikurikiranye, urutonde rwihariye rwa aptamer ihujwe nintego rushobora kugenwa neza, rutanga amakuru yibanze kubisesengura bya aptamer hanyuma bigashyirwa mubikorwa.

Aptamers yakoreshejwe cyane mubushakashatsi bwa siyanse, kuvura indwara, kubaka biosensor, kuvumbura imiti no gukurikirana ibidukikije. Nyamara, aptamers ya oligonucleotide yerekanwe muri vitro yangiritse byoroshye muri vivo ndetse ikanerekana uburozi. Kubwibyo, nyuma yo gutezimbere aptamers yerekanwe, muri vitro isesengura irasabwa gusuzuma intsinzi yiterambere rya aptamer. Ukurikije isesengura ritandukanye ryabakiriya bakeneye, Alpha Lifetech ifite ingamba zitandukanye zo gusesengura aptamer kubakiriya bahitamo.

Igishushanyo.1 Igishushanyo cyo gusesengura aptamer. Inkomoko:Thevendran R, Citartan M. 2022.Isuzuma ryo kugereranya isano ya Aptamers.

Intangiriro kuri serivisi yubushakashatsi bwa Aptamer

Isesengura rya Aptamer

Nuclease igerageza

Mugushyiramo aptamer hamwe nubwoko butandukanye bwa nuclease (nka DNase, RNase, nibindi) mubihe byihariye, iyangirika rya aptamer riragaragara, kugirango dusuzume ubushobozi bwo kurwanya kwangirika. Ibi nibyiza kumenya gutuza no kuramba kwa aptamer mubikorwa bifatika.

Uburyo bwa label ya Fluorescent

Ihame rya aptamer risuzumwa mu buryo butaziguye no gushyiramo fluorophores kuri aptamer no kureba impinduka zerekana ibimenyetso bya fluorescence mbere na nyuma yo guhuza intego. Kurugero, mugihe aptamer ihuza intego, ihinduka ryayo rishobora guhinduka, bikavamo ikimenyetso cya fluorescence cyongerewe cyangwa kigabanutse.

Isesengura ryumuriro

Ubushyuhe bwumuriro wa aptamer busuzumwa mugupima ihinduka ryubushyuhe bwacyo (Tm agaciro) mubushyuhe butandukanye. Ubushyuhe bwo hejuru bwo gushonga bwa aptamer, niko bihagarara neza.

Isesengura rihamye

Ubuso bwa plasmon resonance (SPR) hamwe nubundi buryo bwikoranabuhanga byakoreshejwe mugukurikirana inzira yo guhuza no gukuramo hagati ya aptamer nintego mugihe nyacyo, kubona ibipimo bigenda neza (nko guhuza igipimo gihoraho, igipimo cyo gutandukana guhoraho, nibindi), kurushaho gusobanukirwa nuburyo bwimikoranire hagati ya aptamer nintego, no gusuzuma ihame ryayo rihamye.

Isesengura ryimiterere

X-ray kristalografiya, magnetiki resonance (NMR) hamwe nubundi buryo bwa biologiya bwubatswe bwakoreshejwe mu gusesengura imiterere-yimiterere itatu ya aptamer nuburyo itajegajega.

Isesengura ryihariye rya Aptamer

Aptamer binding assay

Ubusabane busuzumwa no gukora aptamer ihuza ibipimo kugirango bapime guhuza guhoraho hagati ya aptamer na molekile yintego, nko gutandukana guhora Kd. Hasi ya Kd agaciro yerekana isano iri hejuru. Aptamer binding assay irashobora gusuzuma abakandida mubuvuzi bafite ubushobozi buhambaye, hanyuma bagakora ubushakashatsi bwa farumasi na farumasi.

Igerageza ryerekana

Isubiramo ryisubiramo ni uburyo bwo gusuzuma kugirango wirinde vuba molekile zidafite intego ku mubare munini w'abakandida. Isuzuma ryinyuma ryakozwe kugirango ukuremo molekile zidafite uyu mutungo cyangwa imikorere. Urufunguzo rwo guhindura ibizamini ni uguhitamo ibimenyetso byerekana ibimenyetso byerekana ibimenyetso cyangwa imiterere ituma molekile zidafite intego zimenyekana kandi zigakurwaho mugihe cyo gusuzuma. Muri ubu bushakashatsi, guhitamo molekile zitari intego ni ngombwa cyane. Hagomba kwemezwa ko molekile zidafite intego zatoranijwe zihagarariye ibintu bishobora kubangamira isuzumwa rya aptamer kandi ko ibintu bishobora kubangamira bitwikiriwe neza bishoboka.

Ikigeragezo cyo kubuza irushanwa

Mugushyiramo abanywanyi birenze urugero (nka molekile ifite imiterere isa na molekile yintego) kuri sisitemu yo guhuza aptamer na molekile yintego, ihinduka ryubushobozi bwo guhuza aptamer na molekile yintego biragaragara. Niba ubushobozi bwa aptamer bwo guhuza intego ya molekile yagabanutse cyane, byerekana ko aptamer ifite umwihariko.

Rimwe na rimwe, igeragezwa ryo kubuza irushanwa rirashobora gukoreshwa nkigice cyangwa nkigice cyo guhuza ibizamini byo gusuzuma. Kurugero, mugikorwa cyo gusuzuma imiti, ubushobozi bwa molekile yimiti ihuza na poroteyine yagenewe irashobora gusuzumwa nubushakashatsi bwabujijwe guhatanira amarushanwa, hanyuma hakabaho ubushakashatsi bwo gusuzuma bwakoreshejwe mugukuraho ibyo bikoresho bifitanye isano cyane ningirabuzimafatizo zisanzwe cyangwa ingirangingo.

Isesengura rya Aptamer Cytotoxicity

Hariho uburyo butandukanye bwubushakashatsi bwo gusesengura aptamer cytotoxicity, bugenewe gusuzuma ingaruka za aptamers kumibereho ya selile, ikwirakwizwa, cyangwa imikorere.

Uburyo	Intangiriro irambuye	Ibyiza	Ingaruka
Uburyo bwo Kumenya MTT	MTT isuzuma nuburyo bushingiye kubikorwa bya enzyme muri mitochondria ya selile nzima. Dehydrogenase ya succinc muri mitochondria ya selile nzima irashobora kugabanya MTT idasanzwe kugeza kumazi adashobora gushonga yubururu-ibara ry'umuyugubwe wa kirisiti ya Formazan ikayishyira muri selire, mugihe selile zapfuye zidafite imikorere nkiyi. Kurandura kristu ukoresheje dimethyl sulfoxide (DMSO) no kumenya iyinjizwa ryumurambararo wihariye (nka 490nm cyangwa 570nm) kuri enzymoleter irashobora kwerekana mu buryo butaziguye umubare w ingirabuzimafatizo nzima bityo ugasuzuma cytotoxicity ya aptamer.	Kwiyunvikana cyane, ubukungu no korohereza	Imirimo iremereye, ibishishwa kama bishobora kwangiza selile; Gusa ibisubizo byanyuma byubushakashatsi birashobora kuboneka, kandi inzira yuzuye ya cytotoxicity ntishobora kuboneka
Uburyo bwo kumenya CCK-8	Kubara Utugingo ngengabuzima-8 (CCK-8) nuburyo bukomeye cyane, butari radiyo ikora ibara ryerekana amabara. CCK-8 irimo WST-8, igabanywa na dehydrogenase muri mitochondriya ya selile nzima kugirango itange amazi menshi ya elegitoronike ya methyl zan. Ingano y'irangi rya mezan yakozwe ifitanye isano cyane n'umubare w'ingirabuzimafatizo, kandi umubare w'utugingo ngengabuzima dushobora kugaragazwa mu buryo butaziguye no gupima agaciro ko kwinjiza urumuri rwa Mezan ku muhengeri wa 450nm, kugira ngo dusuzume cytotoxicity ya aptamer.	Igikorwa cyoroshye, nta mpamvu yo koza selile, gutahura byihuse, umurongo mugari wo gutahura, ibyiyumvo byinshi, gusubiramo neza, cytotoxicity	Igiciro kinini; Rimwe na rimwe biragoye kumenya niba kugabanuka kwinjirira biterwa no kugabanuka kwingirabuzimafatizo cyangwa igabanuka ryibikorwa bya selile dehydrogenase ubwayo
Uburyo bwo Kumenya LDH	LDH (lactate dehydrogenase) ni enzyme ihamye muri cytoplazme ya selile, kandi iyo membrane yangiritse, LDH irekurwa hanze ya selile. LDH irashobora guhagarika aside ya lactique kugirango ikore pyruvate, hanyuma ikore hamwe na INT (umunyu wa tetrazolium) kugirango ikore ibintu byijimye byijimye. Mugupima iyinjizwa ryayo muburebure bwihariye (nka 490nm), urugero rwangirika kwingirabuzimafatizo rushobora kugaragara, hanyuma cytotoxicity ya aptamer irashobora gusuzumwa.	Irerekana mu buryo butaziguye igipimo cy'urupfu rw'utugingo ngengabuzima, kandi ntigira ibyangiritse ku ngirabuzimafatizo, kandi nta radiyo ikora isotope yanduye	Gukenera gukuramo kenshi selile muri incubator, imikorere iragoye; Gusa ibisubizo byanyuma byubushakashatsi birashobora kuboneka, kandi inzira yuzuye ya cytotoxicity ntishobora kuboneka
Isesengura-Igihe Cyuzuye Cyerekana Ishusho Yisesengura	Ukoresheje igihe-nyacyo kizima cyerekana amashusho, igikoresho cyashyizwe muri incubator kugirango turebe kandi twandike inzira yose yo gukura kwakagari mugihe nyacyo. Cytotoxicity ya aptamers irashobora gusuzumwa hifashishijwe isesengura ryimiterere ya morphologie hamwe nimikurire yimikorere ya selile. Ubu buryo burashobora gutanga videwo nigisubizo cyibikorwa bya cytotoxic mugihe ibidukikije bikura neza.	Irashobora gukurikirana inzira ya cytotoxic mugihe nyacyo, amashusho adasenya, kugabanya kwangirika no kwangiza selile; Byombi videwo nibisubizo byuzuye birahari kubisesengura byimbitse.	Ibiciro byibikoresho ni byinshi kandi bisaba ubuhanga bwumwuga nubushobozi bwo gusesengura amakuru

KACHI

Ibyiza bya serivisi yubushakashatsi bwa Aptamer

Igenzura ryuzuye

Optimized Aptamers hamwe no guhagarara neza

Ubukungu kandi neza. Ibiciro byinshi birushanwe, serivisi nziza

Imbuga zubushakashatsi bwuzuye nuburyo bwinshi bwubushakashatsi

Impuguke zabanjirije kugurisha hamwe na serivisi zunganira nyuma yo kugurisha

Uburambe bukomeye mubisesengura rya aptamer

Itsinda ryo mu rwego rwo hejuru R&D

Serivisi yihariye ya aptamer

Garuka kurupapuro rwiterambere rwa Aptamer

Niba ufite ikibazo, nyamuneka twandikire igihe icyo aricyo cyose.

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