Serivisi y'ubushakashatsi ya Aptamer
Dukurikije ibyo abakiriya bakeneye mu isesengura, Alpha Lifetech ifite ingamba zitandukanye zo gusesengura aptamer abakiriya bashobora guhitamo.
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Serivisi y'ubushakashatsi ya Aptamer
Intangiriro kuri Aptamer
Aptamers ni aside nucleic imwe (nk'iya ADN cyangwa RNA) ipimwa muri vitro iva mu bubiko bwa oligonucleotides zikozwe mu buryo butunguranye hamwe n'uburyo bwitwa SELEX screening, bukubiyemo guhitamo inshuro nyinshi. Ibice by'ingenzi bya serivisi zo guteza imbere aptamer zifitwe na Alpha Lifetech birimo kubaka isomero rya aptamer, gusuzuma aptamer SELEX, gukurikirana aptamer SELEX, gusesengura aptamer sequence ya aptamer n'ubundi buryo bwo gusesengura aptamer.
Uburyo bwo gukurikirana bwa SELEX buhuza uburyo bwo gusuzuma aptamer SELEX n'uburyo bwo gukurikirana ibintu mu buryo bwo hejuru. Binyuze muri aptamer SELEX, uburyo bwihariye bwa aptamer bujyanye n'intego bushobora kumenyekana neza, bigatanga amakuru y'ibanze yo gusesengura no gukoresha uburyo bwa aptamer.
Aptamers zakoreshejwe cyane mu bushakashatsi bwa siyansi, kuvura indwara, kubaka biosensors, kuvumbura imiti no gukurikirana ibidukikije. Ariko, aptamers za oligonucleotide zasuzumwe muri vitro zoroshye kwangirika muri vivo ndetse zikanagaragaza uburozi. Kubwibyo, nyuma yo kunoza aptamers zasuzumwe, isesengura rya vitro rirakenewe kugira ngo harebwe intsinzi y’iterambere rya aptamer. Dukurikije ibyo abakiriya bakeneye mu isesengura, Alpha Lifetech ifite ingamba zitandukanye zo gusesengura aptamers abakiriya bashobora guhitamo.
Igishushanyo cya 1 Ishusho y'isesengura rya aptamer. Inkomoko y'inyandiko:Thevendran R, Citartan M. 2022. Isuzuma ryo gupima isano iri hagati ya Aptamers.
Intangiriro kuri Serivisi y'Ubushakashatsi ya Aptamer
Isesengura ry'Uburimbane bwa Aptamer
Igerageza ryo kwangiza imiterere y'ingufu za kirimbuzi
Mu gushyira aptamer mu buryo butandukanye bwa nucleases (nka DNase, RNase, nibindi) mu bihe runaka, igabanuka rya aptamer rigaragara, kugira ngo harebwe ubushobozi bwayo bwo kurwanya kwangirika. Ibi ni ingirakamaro mu kumenya uburyo aptamer ihagaze neza kandi iramba mu bikorwa byayo.
Uburyo bwo gushyira ibimenyetso ku gikoresho gitanga urumuri
Ubushobozi bwa aptamer bupimwa mu buryo butaziguye hakoreshejwe uburyo bwa fluorophores kuri aptamer no kureba impinduka z'ikimenyetso cy'urumuri mbere na nyuma yo gufatana n'ikintu. Urugero, iyo aptamer ifatanye n'ikintu, imiterere yacyo ishobora guhinduka, bigatuma ikimenyetso cy'urumuri cyiyongera cyangwa kikagabanuka.
Isesengura ry'ubushyuhe buhamye
Ubushyuhe buhamye bwa aptamer bupimwa hapimwa impinduka z'ubushyuhe bwayo bushonga (Tm value) ku bushyuhe butandukanye. Uko ubushyuhe bushonga bwa aptamer burushaho kuba bwinshi, niko ubushyuhe bwayo burushaho gukomera.
Isesengura ry'ubudahangarwa bw'ikirere
Ikoranabuhanga rya plasmon yo hejuru (SPR) n'ubundi buryo bwakoreshejwe mu gukurikirana inzira yo gufatanya no gukuramo hagati ya aptamer n'icyerekezo mu gihe nyacyo, kubona ibipimo bihinduka (nk'igipimo gihoraho cyo gufatanya, igipimo gihoraho cyo gutanya, nibindi), gusobanukirwa neza uburyo bwo gukorana hagati ya aptamer n'icyerekezo, no gusuzuma uburyo ihamye bwayo.
Isesengura ry'imiterere y'imiterere
Gusesengura imiterere y’icyuma gipima imiterere y’izuba (X-ray crystallography), NMR (nuclear magnetic resonance) n’ubundi buryo bwa biology mu miterere byakoreshejwe mu gusesengura imiterere y’icyuma gipima imiterere itatu n’uburyo gihagaze.
Isesengura ryihariye rya Aptamer
Isuzuma ryo gufatanya na Aptamer
Isuzuma ry’ubumwe rikorwa hakoreshejwe isuzuma rya aptamer ripima ubusumbane hagati ya aptamer n’umubumbe w’ingenzi, nka Kd. Agaciro ka Kd gake kerekana ubusumbane buri hejuru. Isuzuma rya Aptamer rishobora gusuzuma imiti ifite ubushobozi bwo gufatanya cyane, hanyuma rigakora inyigo za pharmacodynamics na pharmacokinetics.
Igerageza ryo gusuzuma inyuma
Isuzuma ryo gusuzuma inyuma ni uburyo bwo gusuzuma kugira ngo haveho vuba molekile zitari mu ntego mu mubare munini w’uturemangingo. Isuzuma ryo gusuzuma inyuma rigamije gukuraho molekile zidafite iyi miterere cyangwa imikorere. Icy'ingenzi cyo gusuzuma inyuma ni uguhitamo ibimenyetso cyangwa imiterere ikwiye yo gusuzuma inyuma ituma molekile zitari mu ntego zimenyekana kandi zikavanwaho mu gihe cyo gusuzuma. Muri ubu bushakashatsi, guhitamo molekile zitari mu ntego ni ingenzi cyane. Hagomba kugenzurwa ko molekile zitari mu ntego zatoranijwe zigaragaza ibintu bishobora kubangamira isuzuma rya aptamer kandi ko ibintu bishobora kubangamira bikubiye mu bushakashatsi uko bishoboka kose.
Igerageza ryo gukumira amarushanwa
Mu kongeramo abahanganye cyane (nk'utunyangingo dufite imiterere isa n'iya molekile y'icyerekezo) kuri sisitemu yo gufatanya ya aptamer na molekile y'icyerekezo, hagaragara impinduka mu bushobozi bwo gufatanya bwa aptamer na molekile y'icyerekezo. Iyo ubushobozi bwa aptamer bwo gufatanya na molekile y'icyerekezo bwagabanutse cyane, bigaragaza ko aptamer ifite umwihariko wo hejuru.
Mu bihe bimwe na bimwe, igerageza ryo gukumira indwara rishobora gukoreshwa nk'igice cyangwa nk'inyongera mu igerageza ryo gusuzuma indwara. Urugero, mu isuzuma ry'imiti, ubushobozi bw'umuti bwo gufatana na poroteyine bushobora gusuzumwa hakoreshejwe igerageza ryo gukumira indwara rishobora gusuzumwa, hanyuma igerageza ryo gusuzuma indwara rigakoreshwa mu gukuraho ibyo bintu bifatanye cyane n'uturemangingo cyangwa ingirabuzima fatizo zisanzwe.
Isesengura ry'uburozi bwa Aptamer Cytotoxicity
Hari uburyo butandukanye bwo kugerageza bwo gusesengura uburozi bwa aptamer, bugamije gusuzuma ingaruka za aptamer ku kubaho kw'uturemangingo, kwiyongera kwatwo, cyangwa imikorere yatwo.
| Uburyo | Intangiriro irambuye | Akamaro | Imbogamizi |
|---|---|---|---|
| Uburyo bwo Gutahura MTT | Isuzuma rya MTT ni uburyo bushingiye ku mikorere ya enzyme muri mitochondria y’uturemangingo tuzima. Succinate dehydrogenase muri mitochondria y’uturemangingo tuzima ishobora kugabanya MTT yo hanze ikaba Formazan idafite amazi, ikayishyira mu turemangingo, mu gihe uturemangingo twapfuye nta kazi nk’ako dufite. Gushonga izi kristu hakoreshejwe dimethyl sulfoxide (DMSO) no kumenya uburyo zinjira mu kirere ku bunini bw’urumuri runaka (nka 490nm cyangwa 570nm) kuri enzymoleter bishobora kugaragaza mu buryo butaziguye umubare w’uturemangingo tuzima bityo bigasuzuma uburozi bwa aptamer. | Ubuhanga buhanitse, buhendutse kandi bworoheye abantu | Imirimo myinshi, imiti ihumanya ikirere ishobora kwangiza uturemangingo; ibisubizo bya nyuma by'igerageza ni byo byonyine bishobora kuboneka, kandi inzira yose y'uburozi bw'umubiri ntishobora kugaragara. |
| Uburyo bwo Gutahura CCK-8 | Uburyo bwo kubara uturemangingo-8 (CCK-8) ni uburyo bwo gupima imiterere y’uturemangingo bukoresha ubushobozi bwo kumenya imiterere y’uturemangingo, kandi budafite imirasire. CCK-8 irimo WST-8, igabanuka bitewe na dehydrogenase muri mitochondria y’uturemangingo tuzima kugira ngo ikore lisansi ya methyl zan y’icunga ishongera cyane mu mazi. Ingano y’irangi rya mezan ikorwa ifitanye isano n’umubare w’uturemangingo tuzima, kandi umubare w’uturemangingo tuzima ushobora kugaragara mu buryo butaziguye upimye agaciro k’urumuri rw’irangi rya Mezan ku bunini bwa 450nm, kugira ngo harebwe uburozi bwa aptamer. | Gukora byoroshye, nta mpamvu yo koza uturemangingo, kumenya vuba, kumenya umurongo mugari, kumenya neza, gusubiramo neza, uburozi buke bwa cytotoxicity | Igiciro cyo hejuru cya reagent; Hari igihe bigorana kumenya niba kugabanuka k'umuvuduko w'amaraso biterwa no kugabanuka k'umubare w'uturemangingo tuzima cyangwa kugabanuka k'imikorere ya selile dehydrogenase ubwayo. |
| Uburyo bwo Gutahura LDH | LDH (lactate dehydrogenase) ni enzyme ihoraho muri cytoplasm y'uturemangingo, kandi iyo membrane y'uturemangingo yangiritse, LDH irekurwa hanze y'uturemangingo. LDH ishobora gukurura aside lactic kugira ngo ikore pyruvate, kandi igakorana na INT (tetrazolium salts) kugira ngo ikore ikintu cya crystalline cy'umuhengeri. Mu gupima ubushobozi bwayo bwo kuyifata ku rugero runaka (nk'urugero rwa 490nm), urugero rw'uburyo akaremangingo kangiritse rushobora kugaragara, hanyuma hagasuzumwa uburozi bwa aptamer. | Igaragaza neza igipimo cy'urupfu rw'uturemangingo, kandi nta kwangirika guke ku turemangingo, kandi nta ngaruka zanduza isotope ziterwa n'imirasire | Kuba ukeneye gukura uturemangingo kenshi muri icyo cyuma gishyushya, igikorwa kiragoye cyane; ibisubizo bya nyuma by'igerageza ni byo byonyine bishobora kuboneka, kandi inzira yuzuye y'uburozi bw'uturemangingo ntishobora kugaragara. |
| Isesengura ry'ishusho y'uturemangingo tuzima mu gihe nyacyo | Hakoreshejwe uburyo bwo gusesengura amashusho y’uturemangingo mu buryo bw’igihe nyacyo, igikoresho cyashyizwe muri icyo cyuma kugira ngo kirebe kandi cyandike inzira yose y’imikurire y’uturemangingo mu gihe nyacyo. Uburozi bwa aptamers bushobora gusuzumwa hakoreshejwe gusesengura impinduka mu miterere y’uturemangingo n’imikurire y’uturemangingo. Ubu buryo bushobora gutanga videwo n’ibipimo by’imikurire ya cytotoxic mu gihe ibidukikije by’imikurire y’uturemangingo bigumana umutekano. | Ishobora gukurikirana inzira y’uburozi bwa cytotoxic mu gihe nyacyo, ifoto idasenya, ikagabanya ukwivanga no kwangirika kw’uturemangingo; amashusho n’ibisubizo byapimwe birahari kugira ngo bisesengurwe byimbitse. | Ibiciro by'ibikoresho biri hejuru kandi bisaba ubuhanga mu mikorere n'ubushobozi bwo gusesengura amakuru |
KACHI
Akamaro ka Serivisi y'Ubushakashatsi ya Aptamer
Igenzura ry'Ubuziranenge ryose
Aptamers nziza kandi zifite umutekano mwinshi
Ihendutse kandi ikora neza. Igiciro cyiza kandi serivisi nziza
Ahantu hakorerwa ubushakashatsi bwuzuye n'uburyo bwinshi bwo gukora ubushakashatsi
Serivisi z'ubujyanama bw'inzobere mbere yo kugurisha no gutanga ubufasha nyuma yo kugurisha
Ubunararibonye bwinshi mu isesengura rya aptamer
Itsinda ryo ku rwego rwo hejuru ry’ubushakashatsi n’iterambere
Serivisi zijyanye na aptamer zikozwe ku giti cyazo
Niba ufite ikibazo icyo ari cyo cyose, twandikire igihe icyo ari cyo cyose.
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