Aptamer Yerekana
Alpha Lifetech yashyizeho uburyo bunoze bwo gusuzuma aside nucleic aside aptamer, ishobora guha abakiriya serivisi nziza yo gupima aside nucleic aside (harimo na RNA aptamers na ADN aptamers).
TWANDIKIRE01
Aptamer Yerekana
Ikoranabuhanga rya Aptamer
Aptamers ni ADN imwe ikurikiranye cyangwa RNA ikurikirana, ishobora kugabanwa mubice bitatu. Buri ruhererekane rufite akarere kateganijwe kuri 20-60 nucleotide yegeranye na 5 'na 3' primer zateganijwe, naho blokisiyo ikurikirana yarimo 1015 idasanzwe. Bashoboye guhuza hamwe cyane kandi byihariye kuri molekile zabo, bisa na antibodies. Kugeza ubu, yerekanye ubushobozi bwagutse bwo gukoreshwa mubice byinshi nko kuvura, gusuzuma indwara na biosensor, nibindi.
Alpha Lifetech ifite uburambe bunini mu buhanga bwo gusuzuma ligand, bushingiye ku buhanga bwa Exponential Enrichment Ligand System Evolution (SELEX) bwo guhitamo vitro ligand. Nyuma yimyaka yiterambere, Alpha Lifetech yashyizeho uburyo bwuzuye bwo gusuzuma aside nucleic acide aptamer, ishobora guha abakiriya serivisi nziza yo gusuzuma aside nucleic aside (harimo na RNA aptamers na ADN aptamers). Intego nyinshi zo gusuzuma nka proteyine, peptide, aside amine, ibice bito bya molekile, hamwe nuburyo butandukanye bwo gusuzuma aptamer (nk'isaro rya magneti SELEX, selile SELEX, gufata SELEX, nibindi) birahari. Uburyo bukoreshwa mugusuzuma ni magnetiki yerekana amasaro.
Uburyo bwa tekinike ya Aptamer Selex Yerekana
Uburyo bwa tekinike ya SELEX yo gusuzuma aptamer itangirana no kubaka amasomero ya oligonucleotide agizwe na ADN cyangwa RNA. Iterative cycle yo gutoranya no kongera imbaraga byagezweho mugushyiramo isomero rya oligonucleotide hamwe na molekile yagenewe, gukaraba oligonucleotide idahumanye, kuvanaho aptamers ihambiriye, kongera aptamers ihambiriwe na PCR kugirango bibyare isomero rya kabiri mugice gikurikira cyo gusuzuma. Subiramo intambwe zo guhambira, gutandukana, gukira, no kongera kwongera. Urutonde rwihariye rukungahaye muriyi nzira rugaragara nka aptamers yiganje mu isomero.
Igishushanyo.1 SELEX Yerekana Uburyo bwa Tekinike
ibyiza bya acide Nucleic aptamer
| Ibyiza | Ibisobanuro |
|---|---|
| Bitaziguye | Kwirinda inzira gakondo yubushakashatsi bwinyamaswa no guhitamo biturutse mumasomero ya vitro; isuzuma rya selex kuri aptamers ya molekile yintego itari immunogene, immunogenic nke cyangwa nuburozi. |
| Urwego runini rwa porogaramu | Intego ya molekile irashobora kuba amarangi kama, aside amine, proteyine, antibiotike, peptide, vitamine, ibiyobyabwenge, ndetse selile, virusi, virusi, tissue, nibindi. |
| Gukomera cyane | Aptamers yerekana umwihariko hamwe nubusabane muri vitro yerekana vitro ya molekile. |
| Igihagararo | Aptamers ni ntoya mubunini, yoroshye kuyibona, yororoka muri synthesis, kandi ituze ryayo irashobora kuzamurwa na synthesis ya chimique no guhindura. Aptamers ihagaze neza mumiti, ifite ubuzima burebure kandi irashobora gutwarwa mubushyuhe bwicyumba. |
Isesengura ry'ubuzima
Koresha aptamers nka probe cyangwa biosensor kugirango ukore molekile ya biologiya, amashusho ya selile nubundi bushakashatsi bwisesengura ryubuzima.
Gusuzuma Indwara
Gutezimbere uburyo bworoshye bwo gusuzuma indwara bushingiye ku guhuza kwihariye kwa aptamers na molekile zigamije.
Ubushakashatsi mu buvuzi
n'iterambere
Koresha aptamer yatoranijwe nka molekile yimiti cyangwa abatwara imiti kugirango utezimbere imiti mishya.
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2018-07-16 
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