Ihuriro rya Aptamer

Intangiriro kuri acide Nucleic aptamers

Nucleic acide aptamers, izwi kandi nka aptamers, complexes, na antibodiyite za chimique, ziboneka mugupima vitro selex kwerekanwa na tekinike ya ligand exponential enrichment system (SELEX). Ni acide deoxyribonucleic acide imwe (ssDNA) cyangwa aside ribonucleic (RNA) ihuza byumwihariko kandi neza na molekile igenewe, kandi mubisanzwe igizwe na base 20-80.

Nucleic acide aptamers yabonetse mugusuzuma vitro irahagaze neza, yoroshye guhuza kandi byoroshye kuyihindura, kandi iruta antibodiyide zikoreshwa cyane mubinyabuzima mubijyanye na synthesis hamwe nogukoresha; hiyongereyeho, acide nucleic aptamers irashobora guhuza cyane cyane nintego zabo binyuze mumbaraga zinyuranye zitandukanye, muri rusange, zikorana na molekile ntoya ishingiye kumurongo mugari (urugero, imisatsi, conve-convex, pseudo-twisted, nibindi) binyuze mumazi ya hydrogène, imbaraga za electrostatike, imikoranire ya hydrophobique, nibindi bya molekile. Ukurikije intego, aptamers acide nucleic irimo ADN aptamers, RNA aptamers, protein aptamers, na aptamers selile.

Alpha Lifetech ya synthesis ya aptamer, ikubiyemo cyane cyane serivisi ya synthesis ya SELEX aptamer hamwe na serivise yiterambere rya aptamer (ADN, RNA cyangwa XNA), hamwe no kwerekana aptamer yo hepfo, kugenzura aptamer, hamwe na serivisi zisesengura za aptamer.

Inzira ya tekiniki ya synthesis ya Aptamer

Ihame shingiro hamwe nuburyo bwikoranabuhanga

Inzira ya nucleic acide aptamer synthesis ni uko isomero rya oligonucleotide imwe ihuriweho na chimique ikomatanya muri vitro, ikavangwa nibintu bigenewe, hamwe na complexe yibintu bigenewe hamwe na acide nucleique biboneka muruvange, acide nucleic idahuza nibintu byateganijwe irakaraba, kandi molekile ya acide nucleic ihuza na molekile ya acide ya nucleique ya selile. Binyuze mu isuzuma rya selex inshuro nyinshi no kongera imbaraga, molekile zimwe na zimwe za ADN cyangwa RNA zidahuza nibintu byateganijwe cyangwa zifitanye isano rito cyangwa ziciriritse kubintu byagenewe zizahanagurwa, kandi aptamers, ni ukuvuga ADN cyangwa RNA ifitanye isano n’ibintu bigenewe cyane, kandi ubuziranenge bwabo buziyongera hamwe n’ibikorwa bya SELEX, kandi amaherezo bizatwara igice kinini cy’isomero.

Uburyo bwa Tekinike Ingingo z'ingenzi

Igikorwa cyo gusuzuma selex ya nucleic acide aptamers itangirana no guteranya imiti yububiko bwa chimique ya oligonucleotide. Amasomero asanzwe ya oligonucleotide afite ubushobozi bunini bwibitabo. Ubwinshi butandukanye ninyungu zingenzi. Mubyukuri, niba urukurikirane rudasanzwe muri oligonucleotide rugizwe n shingiro, ubushobozi bwibitabo ni 4n. Turamutse dusuzumye isomero ryahinduwe ryibitabo, bizongera ubudasa bwikurikiranya. Kugeza ubu, uburebure bwikurikiranya bwa oligonucleotide bukoreshwa mubushakashatsi bwa SELEX muri rusange ni 30 shingiro, kandi ubushobozi bwibitabo bushobora kuba hejuru ya 10 ^ 18. Ubushobozi bwibitabo nibyingenzi mugusuzuma selex yo hejuru-ligande. Nyamara, kubera ko kwibumbira mu masomero yubukorikori ari bimwe, birebire cyane bikurikiranye bizagabanya ubwinshi bwa buri oligonucleotide mu masomero, bizagabanya amahirwe yo gusuzuma selex kuri aptamer runaka. Mubisanzwe, isomero ryubushobozi bwa 10 ^ 13 ~ 10 ^ 15 mugice cya mbere cyo gusuzuma selex irashobora guhaza ibikenewe bifatika.

Igishushanyo.1 SELEX Inzira ya tekiniki

ibyiza bya acide Nucleic aptamer

Ibyiza	Ibisobanuro
Bitaziguye	Kwirinda inzira gakondo yubushakashatsi bwinyamaswa no guhitamo biturutse mumasomero ya vitro; isuzuma rya selex kuri aptamers ya molekile yintego itari immunogene, immunogenic nke cyangwa nuburozi.
Urwego runini rwa porogaramu	Intego ya molekile irashobora kuba amarangi kama, aside amine, proteyine, antibiotike, peptide, vitamine, ibiyobyabwenge, ndetse selile, virusi, virusi, tissue, nibindi.
Gukomera cyane	Aptamers yerekana umwihariko hamwe nubusabane muri vitro yerekana vitro ya molekile.
Igihagararo	Aptamers ni ntoya mubunini, yoroshye kuyibona, yororoka muri synthesis, kandi ituze ryayo irashobora kuzamurwa na synthesis ya chimique no guhindura. Aptamers ihagaze neza mumiti, ifite ubuzima burebure kandi irashobora gutwarwa mubushyuhe bwicyumba.

// Nucleic aside aptamer ikoreshwa //

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Ubuvuzi bwa Clinical: intego yo kuvura ibibyimba, tekinike yo gusuzuma kanseri

Raporo yinguzanyo ya Gladtrust yubatswe neza hashingiwe kububiko bwamakuru yinguzanyo ya businessonline - CreditVision.
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Isesengura ryisesengura: gutahura ibyuka bihumanya, gutahura ibiyobyabwenge bito bya molekile, kumenya indwara ziterwa na virusi

Muri uru rwego, tekinoroji ya nucleic aside aptamer synthesis izakoreshwa ifatanije na immunochromatografi ya zahabu ya colloidal, aho izahabu ya colloidal izajya itegurwa kandi ikamenyekana neza, hanyuma igahuzwa na acide nucleic aptamer, ikarangwa kandi igaterwa kuri padi ihuza, hanyuma ibice byipimisha bikazategurwa hifashishijwe umurongo wa antibody kugirango ugenzure umurongo wa nucleique kugirango ugenzure umurongo wa nucleque. intego yibintu hashingiwe kubisubizo byubushakashatsi bubi kandi bwiza.

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Umwanya wibinyabuzima: iterambere rya biosensor

Nucleic acide aptamer sensor ikoresha aptamer nkikintu cyo kumenyekanisha ibintu runaka no guhindura ibisubizo byamenyekanye mubimenyetso bishobora gupimwa. Guhindura ibimenyetso birashobora kuba amashanyarazi, optique, ubushyuhe, piezoelectric, magnetic na micromechanical cyangwa guhuza ubwo buhanga. Ugereranije nuburyo buriho bwo gutahura, ibyuma bya nucleic acide aptamer bishingiye kuri sensororo muri rusange biroroshye gukora, byihariye, byitabirwa kandi byoroshye, kandi izi nyungu zorohereza gukoreshwa mubitegererezo nyabyo kandi bifite ibyiringiro byiza byiterambere.