Urubuga rwa Aptamer Synthesis
Bitewe n'icyo umuntu ashaka, aptameri za aside nucleic zirimo aptameri za ADN, aptameri za RNA, aptameri za poroteyine, na aptameri za membrane y'uturemangingo.
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Urubuga rwa Aptamer Synthesis
Intangiriro ku bijyanye na aptameri za aside nucleic
Aptameri za aside nucleic, zizwi kandi nka aptameri, complexes, na antibodies za chimique, ziboneka hakoreshejwe uburyo bwa "in vitro selex screening" hakoreshejwe tekiniki ya "ligand exponential enrichment system evolution" (SELEX). Ni aside deoxyribonucleic acid" ifite umugozi umwe (ssDNA) cyangwa aside ribonucleic acid (RNA) ifatana by'umwihariko kandi neza na molekile, kandi ubusanzwe igizwe n'ibice 20-80.
Aptameri za aside nucleic ziboneka hakoreshejwe uburyo bwa "in vitro screening" zirahamye mu miterere yazo, zoroshye kuzivanga kandi zoroshye kuzihindura, kandi ziruta abasirikari basanzwe bakomoka kuri biyolojiya mu bijyanye no kuzivanga no kuzikoresha; byongeye kandi, aptameri za aside nucleic zishobora kwihuza neza n'intego zazo binyuze mu mbaraga zitandukanye, muri rusange, zikorana na molekile nto hashingiwe ku miterere itandukanye (urugero: udupira tw'imisatsi, concave-convex, pseudo-twisted, nibindi) binyuze mu guhuza hydrogen, imbaraga za electrostatic, hydrophobic interactions, nibindi. Bitewe n'icyo zigamije, aptameri za aside nucleic zirimo aptameri za ADN, aptameri za RNA, aptameri za poroteyine, na aptameri za membrane y'uturemangingo.
Urubuga rwa Alpha Lifetech rwo guhuza aptamer, rugizwe ahanini na serivisi ya SELEX aptamer isomero na serivisi yo guteza imbere aptamer (DNA, RNA cyangwa XNA), ndetse no gusuzuma aptamer, gukoresha aptamer neza, na serivisi zo gusesengura inyito ya aptamer.
Uburyo bwa tekiniki bwo guhuza Aptamer
Ihame ry'ibanze n'inzira y'ikoranabuhanga
Uburyo bwo gukora ikoranabuhanga ryo guhuza aside nucleic aptamer ni uko isomero rya oligonucleotides imwe ikorwa mu buryo bwa shimi, ikavangwa n’ikintu kigamije, kandi urusobe rw’ikintu kigamije na aside nucleic ruri mu ruvange, aside nucleic idafatana n’ikintu kigamije irakurwaho, kandi molekile za aside nucleic zifatana n’ikintu kigamije irakurwaho, kandi molekile za aside nucleic zikoreshwa nk’ibishushanyo mbonera byo gukora PCR amplification mu cyiciro gikurikira cyo gusuzuma. Binyuze mu gusuzuma no kongera ingufu za selex kenshi, molekile zimwe na zimwe za ADN cyangwa RNA zidafatana n’ikintu kigamije cyangwa zifite affinity nto cyangwa iringaniye ku kintu kigamije irakurwaho, kandi molekile, ni ukuvuga ADN cyangwa RNA ifite affinity nyinshi ku kintu kigamije irakurwaho, zizatandukanywa n’isomero rinini cyane, kandi ubuziranenge bwazo buziyongera uko SELEX igenda, kandi amaherezo zizafata igice kinini cy’isomero.
Ingingo z'ingenzi z'Inzira ya Tekiniki
Uburyo bwo gusuzuma selex aptamers za aside nucleic butangirana no guteranya amasomero ya oligonucleotide yakozwe mu buryo bwa shimi. Amasomero ya oligonucleotide ya random afite ubushobozi bwinshi bwo kubika amakuru. Ubudasa bwinshi ni inyungu yabwo ikomeye. Mu buryo bw'imitekerereze, niba urukurikirane rw'ibintu muri oligonucleotide rugizwe n'ibice bya n, ubushobozi bw'isomero ni 4n. Iyo turebye isomero rya artificial modification, byongera ubudasa bw'ibintu bya random sequences. Muri iki gihe, uburebure bw'urukurikirane rw'ibintu bya oligonucleotide bikunze gukoreshwa mu bushakashatsi bwa SELEX muri rusange ni ibice 30, kandi ubushobozi bw'isomero bushobora kugera kuri 10^18. Ubushobozi bw'isomero ni ingenzi mu gusuzuma selex ligands za high-affinity. Ariko, kubera ko ubwinshi bw'amasomero ya sintetike ari ingenzi, urukurikirane rurerure cyane ruzagabanya ubwinshi bwa buri oligonucleotide mu masomero, bizagabanya amahirwe yo gusuzuma selex kuri aptamer runaka. Muri rusange, ubushobozi bw'isomero bwa 10^13~10^15 mu cyiciro cya mbere cyo gusuzuma selex bushobora guhaza ibikenewe mu buryo bufatika.
Igishushanyo cya 1 Inzira ya tekiniki ya SELEX
ibyiza bya aside nucleic aptamer
| Ibyiza | Ibisobanuro birambuye |
|---|---|
| Inzira itaziguye | Kwirinda inzira gakondo yo kugerageza inyamaswa no guhitamo mu bubiko bwa "in vitro"; gusuzuma "selex" aptamers" z'uturemangingo tw’ingenzi tudatera ubudahangarwa bw’umubiri, tudatera ubudahangarwa bw’umubiri cyangwa dufite uburozi. |
| Urutonde rugari rw'ibikorwa | Molekile zigomba kuba ari irangi ry’umwimerere, aside amine, poroteyine, antibiyotike, peptides, vitamine, imiti, ndetse n’uturemangingo, indwara zitera indwara, virusi, ingirabuzima fatizo, nibindi. |
| Ubwumvikane Bukomeye | Aptamers zigaragaza ubuhanga n'ubuhanga bwinshi mu gusuzuma molekile zigamije mu buryo bwa vitro. |
| Ituze | Aptameri ni ntoya, zoroshye kuzibona, zishobora kongera gukoreshwa mu gukora, kandi ubushobozi bwazo bushobora kwiyongera binyuze mu gukora no guhindura imiti. Aptameri ntizihinduka mu buryo bwa shimi, zimara igihe kirekire kandi zishobora gutwarwa ku bushyuhe bw'icyumba. |
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Muri uru rwego, ikoranabuhanga rya aside nucleic aptamer rizakoreshwa hamwe na colloidal gold immunochromatography, aho zahabu ya colloidal izabanza gutegurwa no kumenyekana ubuziranenge, hanyuma igahuzwa na aside nucleic aptamer, ikagaragazwa kandi igaterwa ku gapapuro ko gufatanya, hanyuma uduce tw’ibizamini tuzategurwa hakoreshejwe antibody ya polyclonal izaboneka nk'umurongo wo gupima, n'uruhererekane rwuzuzanya rwa aside nucleic aptamer nk'umurongo wo kugenzura ubuziranenge, kugira ngo hasesengurwe niba hari ikintu kigomba kwitabwaho hashingiwe ku bisubizo by'igerageza ribi n'iry'ibyiza.
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Urubuga rw'ibinyabuzima: iterambere ry'ibipimo ngenderwaho by'ibinyabuzima
Utwuma dupima aside nucleic aptamer dukoresha aptamer nk'ikintu cyo kumenya ikintu runaka no guhindura umusaruro wo kumenya ikimenyetso gishobora gupimwa. Ihinduka ry'ibimenyetso rishobora kuba electrochemical, optique, ubushyuhe, piezoelectric, magnetic na micromechanical cyangwa uruvange rw'ubu buryo. Ugereranije n'uburyo busanzwe bwo kumenya, utwuma dupima aside nucleic aptamer muri rusange tworoshye gukoresha, twihariye, twitabira kandi twumvikana, kandi izi nyungu zituma byoroha kubikoresha mu ngero nyazo kandi bifite amahirwe meza yo gutera imbere.
Serivisi yo gukora SELEX Aptamer Synthesis
Serivisi ya Alpha Lifetech yo guhuza aptamer ya SELEX irimo kubaka isomero rya SELEX aptamer, gutunganya SELEX, nibindi. Alpha Lifetech igamije kubona aptamer za SELEX zijyanye n’uburyo bworoshye kandi zihariye ku bakiriya binyuze mu gikorwa cyo gusuzuma in vitro.
SOMA BYINSHI
Serivisi yo guteza imbere aderi ya nucleique
Serivisi yo guteza imbere aside nucleic aptamer ya Alpha Lifetech ishingiye ahanini ku ikoranabuhanga rya SELEX ryo gusuzuma aptamer ya SELEX, harimo guteza imbere aptamers za ADN, aptamers za RNA na aptamers za XNA, harimo gushushanya isomero, guhuza oligonucleotide, kubaka isomero no kugenzura.
SOMA BYINSHINiba ufite ikibazo icyo ari cyo cyose, twandikire igihe icyo ari cyo cyose.
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