Bispecific Antibody Iterambere

Alpha Lifetech irashobora guha abakiriya uburyo bwiza bwo kuvumbura antibody hamwe nibicuruzwa bya poroteyine bya recombinant. Turashobora gutegura antibodies zifite imbaraga nyinshi, umwihariko, hamwe no guhagarara neza. Alpha Lifetech ifite ibikoresho bitandukanye byo kweza antibody n'ibikoresho, bishobora gutanga serivisi zo kweza antibody biva ahantu hatandukanye nk'urukwavu, intama, inkoko, n'imbeba za monoclonal antibodies, hamwe na serivisi zo kweza poroteyine A / G hamwe na serivisi zo gutandukanya antibody no kweza. Dushingiye kuri sisitemu yuzuye yububiko bwa antibody yo kuvumbura antibody, urubuga rwa poroteyine, nibindi, turareba serivisi zo hejuru no hepfo yumusaruro wa antibody, kandi dushobora gutanga serivise tekinike kuva gutegura antibody, kweza bispecific antibody no gutandukanya antibody no kweza, gukurikiranwa kwa antibody, kwemeza antibody, nibindi, bishobora gukoreshwa mubuvuzi bwa antibody.

Antibodiyite za Monoclonal zivuga immunoglobuline ikorwa na selile imwe B ifite umwihariko wa antigene cyangwa epitopi. Umusemburo wa antibodiyumu ya Monoclonal wateguwe bwa mbere ukoresheje tekinoroji ya hybridoma kugirango ubyare antibodies. By'umwihariko, muguhuza ingirabuzimafatizo ziva mu mbeba zakingiwe hamwe na selile myeloma ziva mu bantu cyangwa imbeba, ingirabuzimafatizo za Hybridoma ziba zikora antibodi zihariye. Imbeba zakozwe na antibodiyite zikoreshwa cyane mubushakashatsi bwinyamaswa no gusuzuma indwara nyuma ya serivisi yo kweza. Nyamara, umubiri wumuntu uzatanga ubudahangarwa bw'umubiri wa poroteyine zo mu mahanga, bityo rero hari imbogamizi zikomeye mugukoresha kwa muganga imikoreshereze ya antibodiyite yimbeba. Nyuma, abantu bakoresheje "ubumuntu" kugirango bategure antibodiyite za monoclonal ziva ku mbeba, kandi bakoresha tekinoroji yubuhanga mu bya genetike kugirango bahindure antibodi yimbeba kugira akarere gahoraho ka immunoglobuline kugirango bagabanye ubudahangarwa bwabo. Ubu bwoko bwa antibody buzwi kandi nka antibody ya monoclonal muntu. Byongeye kandi, gukoresha selile zabantu kugirango ubyare antibodiyite bita monoclonal antibody yumuntu. Antibodiyite za Monoclonal zikoreshwa cyane mubikorwa bya biomedical na clinique. Mu bushakashatsi bw’ubuvuzi, antibodiyite za monoclonal zirashobora gukoreshwa nka immunomodulator, antibodiyite zo kuvura monoclonal antibodies zirashobora gukoreshwa mugukoresha kanseri ya chimiotherapie no kuvura virusi, kandi iterambere rya antibody ya monoclonal ikoreshwa cyane mukuvura kanseri.

Intangiriro kuri Antibody Bispecific

Mu 1960, hashyizweho igitekerezo cya antibodiyite za bispecific. Antibodiyisi ya Bispecific, izwi kandi nka antibodiyite ya bispecific monoclonal antibodies, ni synthèse artificiels antibodies ikoresheje tekinoroji yubuhanga. Nka antibody yakozwe na injeniyeri, antibodiyite ya bispecificateur isanzwe iba iy'igice cya IgG kandi ikubiyemo antigen ihuza igice cyibasira CD3 subunit. Antibodiyisi zifite ibice bibiri byihariye bihuza antigen zishobora icyarimwe guhuza no kumenya antigene ebyiri zitandukanye, cyangwa epitopi ebyiri zitandukanye za antigen. Ugereranije na antibodiyite za monoclonal, antibodies zifite bispecificique zifite urubuga rwihariye rwo guhuza antigen, bityo rukaba rufite umwihariko ukomeye hamwe nubushobozi bwo kwibasira, rushobora kwibasira neza selile yibibyimba no kugabanya uburozi bwintego. Antibodies zidasanzwe zirashobora icyarimwe gukora imirimo myinshi yibinyabuzima, nko kwinjiza ingirabuzimafatizo, guhagarika inzira zerekana ibimenyetso, no kwica selile yibibyimba. Antibodiyite zambere za bispecificite zateguwe cyane cyane binyuze mumiti ya conjugation cyangwa fonction selile, ariko ubu buryo bushobora kuba bwarateye imbere buhoro buhoro bitewe no guhuza bidasanzwe hamwe nikibazo cyo gutandukanya intego. Hamwe niterambere ryiterambere rya tekinoroji yubuhanga, hashyizweho uburyo bushya bwikoranabuhanga bushya, nk'amapfundo mu mwobo (KIH), CrossMab, DVD Ig, n'ibindi.

Igishushanyo cya 1 Igishushanyo mbonera cyerekana uburyo bwateganijwe bwo gukora antibodiyite (bsAbs) mu bigeragezo bivura indwara ya onkologiya.(Inkomoko y'ishusho: Isubiramo rya antibodiyite ya bispecificique na antibody yubaka muri oncology nibibazo byubuvuzi - ScienceDirect)

Ikoreshwa rya Antibodies Bispecific

Uburyo nyamukuru bwo gukora antibody ya bispecificique harimo guhuza imiti, imvange enye ya Hybridoma, hamwe no gutegura antibody ya genetique. Muri byo, uburyo bwo guhuza imiti ihuza ibice bibiri bya IgG cyangwa bibiri bya F (ab ') ibice 2 bya antibody muri antibodiyite ya bispecificique ikoresheje imiti ihuza imiti nka phthalimide na aside dithioacylbenzoic. Ubu buryo buroroshye kandi bworoshye gukora, ariko burashobora kwangiza urubuga rwa antigen, kugabanya ibikorwa bya antibody, kandi agent ihuza ubwayo nayo ifite urwego runaka rwa kanseri. Uburyo bune buturuka kuri hybridoma bushingiye ku guhuza ingirabuzimafatizo za somatike kuva kumirongo ibiri itandukanye ya hybridoma kugirango igaragaze imbeba ihuye IgG. Binyuze mu buhanga bwa tekinoroji, antibodies zirashobora guhindurwa genetike kugirango zibe antibodies zidasanzwe. Hubatswe antibodi ebyiri zitandukanye za monoclonal, hanyuma ibice bya Fab cyangwa urunigi ruremereye hamwe nuduce twinshi duhinduranya uturere twa antibodi zombi zashizwe hamwe. Binyuze mu guhuza ibikorwa cyangwa tekinoroji yo guhuza urunigi, ibice byombi byahujwe no gukora antibody idasanzwe. Nubwo ubuhanga bwa tekinoroji yubuhanga bugereranije ni hejuru, kuri ubu ni bwo buryo bukoreshwa cyane mu gukora antibody ya bispecific kugirango ihindure imiterere n'imikorere ya antibodi. Mugihe ukora antibody ya bispecificite, hashobora gukoreshwa ihame rya antibody cross reaction, ariko antibody cross reaction irashobora gutera reaction zidasanzwe, bityo rero izasuzumwa neza mugukoresha muburyo bwa antibody ya bispecificique, nka antibody ya bispecificique.

Bispecific Antibodies Isukura

Bispecific antibody yoza ni inzira yo gutandukanya no kweza antibodiyite zifite isuku nyinshi. Uburyo bubiri, centrifugation hamwe no kuyungurura byimbitse, bikoreshwa mugukuraho umwanda udashonga. Intego ya bispecific antibody yafashwe bwa mbere na affinity chromatography. Kuri IgG nka antibodiyite ya bispecificique, proteine Ikoreshwa rya chromatografi ya affinity ikoreshwa, mugihe kubitari IgG nka antibodiyite ya bispecific, urumuri rwumucyo rushingiye kuri chromatografiya. Nyuma yaho, antibody yatewe mugihe gito cya pH mugihe runaka, bigatuma imiterere ya proteyine hejuru y ibahasha ya virusi ihungabana, bityo igatakaza ubushobozi bwo kwanduza selile. Hagati yo gushungura byimbitse birakorwa kugirango bikureho umwanda nka poroteyine zakira (HCP). Isuku ya antibodies itezimbere binyuze muburyo nka ion yo guhana chromatografiya, kandi virusi zose zisigaye zikurwaho hakoreshejwe nanofiltration cyangwa tekinoroji ya ultrafiltration. Hanyuma, icyitegererezo cyibanze kandi gisimbuzwa na buffer ikwiye.

Bispecific Antibodies Yumusaruro Serivisi Yakazi

Intambwe	Ibirimo	Igihe ntarengwa
Gene Synthesis	Igisekuru cyibishushanyo bigera kuri 3 bitandukanye byuburyo bukurikirana, de novo ibisekuruza bya recombinant antibody ADN plasmide	Ibyumweru 2-3
Ikizamini gito	Umusaruro wa poroteyine ya bispecificique → Imvugo ntoya mumirongo y’inyamabere → Kwemeza na SDS-URUPAPURO → Guhuza isesengura hamwe na poroteyine za antombine za recombinant na ELISA → Kugereranya clone na antibodi z'ababyeyi.	Ibyumweru 5-6
Menya	Kugaragaza antibodiyite ebyiri zambere zuzuye zuzuye → Isesengura rihuza na ELISA → ELISA isuzuma rya antibodi zemewe na antibodies	Icyumweru 1
Antibody Umusaruro	Umusemburo munini wa antibody	Ibyumweru 3-4

Ibyiza byacu

Dutwikiriye serivise zo hejuru no kumanuka zo gukora antibody, kandi turashobora gutanga serivise tekinike kuva gutegura antibody, bispecific antibody yoza no gutandukanya antibody no kweza, gukurikiranwa kwa antibody, kwemeza antibody, nibindi, bishobora gukoreshwa mubuvuzi bwa antibody.

Kugaragaza cyane

Imvugo ya poroteyine nyinshi muri selile CHO.
Ububiko buhamye

> Ibyumweru 2 muri serumu kuri 37 ° C.
Nta kibazo cyo gukemura

> 30 mg / ml
Akagari gahamye

Imirongo myinshi ihamye ya selile hamwe na antigene yibibyimba byakozwe kugirango yemeze antibodies nyinshi