Mu 1960, hatanzwe igitekerezo cy’uturemangingo tw’umubiri twihariye. Uturemangingo tw’umubiri twihariye, tuzwi kandi nka utwo turemangingo tw’umubiri twihariye, ni utwo turemangingo tw’umubiri twihariye hakoreshejwe ikoranabuhanga rya genetiki. Nk’uturemangingo tw’umubiri twihariye, utwo turemangingo tw’umubiri twihariye akenshi tuba mu itsinda rya IgG kandi tukaba dufite agace k’udusimba twinjira mu mubiri twinjira mu gice cya CD3. Uturemangingo tw’umubiri twihariye dufite ahantu habiri hihariye ho gufatanya utwo turemangingo dushobora gufatanya no kumenya utwo turemangingo tubiri dutandukanye icyarimwe, cyangwa epitopes ebyiri zitandukanye za antigen. Ugereranije n’uturemangingo tw’umubiri twihariye, utwo turemangingo tw’umubiri twihariye dufite ahantu hihariye ho gufatanya utwo turemangingo, bityo tukaba dufite ubushobozi bukomeye bwo kwibanda ku turemangingo tw’umubiri no kugabanya uburozi bw’uturemangingo. Uturemangingo tw’umubiri twihariye dushobora gukora imirimo myinshi ya biological icyarimwe, nko gushaka uturemangingo tw’ubudahangarwa bw’umubiri, kuziba inzira zo gutanga ibimenyetso, no kwica uturemangingo tw’umubiri mu buryo butaziguye. Uturemangingo tw’umubiri twihariye twa mbere twateguwe ahanini binyuze mu guhuza imiti cyangwa guhuza uturemangingo, ariko ubu buryo bushobora kuba bwarateye imbere buhoro buhoro bitewe n’uruvange rw’ibintu n’ingorane mu gutandukanya uruvange rw’ibintu. Bitewe n’iterambere rihoraho ry’ikoranabuhanga mu by’ubuhanga mu by’uturemangingo, hakozwe urubuga rwinshi rushya rw’ikoranabuhanga, nko gushyiramo uduce duto (KIH), CrossMab, DVD Ig, nibindi. Izi urubuga zikemura neza ibibazo nko kudahuza kw’imiyoboro minini n’imiyoboro yoroheje, kandi zikongera uburinganire n’umusaruro w’uturemangingo tw’umubiri twihariye.

Uburyo bw'ingenzi bwo gukora antibody ebyiri zihariye burimo guhuza imiti, hybridoma enye zikomoka ku nkomoko, no gutegura antibody y’ubuhanga mu by’imiterere y’uturemangingo. Muri bwo, uburyo bwo guhuza imiti buhuza ibice bibiri bya antibody bya IgG cyangwa bibiri bya F (ab ') 2 mu barwanya antibody ebyiri zikoresha imiti ihuza nka phthalimide na aside dithioacylbenzoic. Ubu buryo bworoshye kandi bworoshye gukoresha, ariko bushobora kwangiza aho antigen ihurira, bukagabanya imikorere y’antibody, kandi igikoresho cyo guhuza ubwacyo gifite urwego runaka rw’indwara ya kanseri. Uburyo bune bwa hybridoma bushingiye ku guhuza uturemangingo two mu mubiri duturutse ku mirongo ibiri itandukanye y’uturemangingo twa hybridoma kugira ngo tugaragaze IgG y’imbeba ijyanye nayo. Binyuze mu ikoranabuhanga rya genetiki, antibody zishobora guhindurwa mu buryo bw’uturemangingo kugira ngo zikore antibody ebyiri zihariye. Antibody ebyiri zitandukanye za monoclonal zakozwe, kandi ibice bya Fab cyangwa ibice biremereye by’uruhererekane rw’iminyururu n’uruhererekane rw’iminyururu ibiri byaciwemo ibice bitandukanye. Binyuze mu ikoranabuhanga ryo guhuza cyangwa guhuza uruhererekane, ibice bibiri byahujwe kugira ngo bikore antibody ebyiri zihariye. Nubwo ikoranabuhanga ry’ubuhanga mu by’imiterere y’uturemangingo (genetic engineering) riri hejuru cyane, ubu ni bwo buryo bukoreshwa cyane mu gukora antibody ebyiri zihariye kugira ngo hakosorwe imiterere n’imikorere y’antibody. Mu gihe cyo gukora igishushanyo mbonera cy’antibody ebyiri zihariye, ihame ryo gukora antibody imwe rishobora gukoreshwa, ariko gukora antibody imwe bishobora gutera ingaruka zitari zimwe, bityo bizasuzumwa neza mu ikoreshwa ry’igishushanyo mbonera cy’antibody ebyiri zihariye, nko kuvura antibody ebyiri zihariye.

Gutunganya antibody ya Bispecific ni inzira yo gutandukanya no gusukura antibody zigamije ubuziranenge buhanitse. Uburyo bubiri, gupima no kuyungurura mu buryo bwimbitse, bukoreshwa mu gukuraho imyanda imwe n'imwe ishongesha. Antibody ya bispecific ibanza gufatwa na affinity chromatography. Kuri antibody za IgG nka bispecific, protein A affinity chromatography irakoreshwa, mu gihe kuri antibody zitari IgG nka bispecific, light chain based affinity chromatography irashobora gukoreshwa. Nyuma, antibody ishyirwa mu buryo bwa pH nke mu gihe runaka, bigatuma imiterere ya poroteyine iri ku buso bw'agakoko gatera virusi ihungabana, bityo igatakaza ubushobozi bwo kwanduza uturemangingo. Gutunganya mu buryo bwimbitse hagati bikorwa kugira ngo hakorwe ibindi bihumanya nka poroteyine za host cell (HCP). Ubuziranenge bwa antibody burushaho kunozwa binyuze mu buryo nka ion exchange chromatography, kandi virusi zisigaye zikurwaho binyuze mu buryo bwa nanofiltration cyangwa ultrafiltration. Amaherezo, icyitegererezo gishyirwa hamwe kandi kigasimbuzwa formulation buffer ikwiye.