Ihuriro rya tekinoroji ya Hybridoma

Alpha Lifetech ifite uburambe bukomeye mubijyanye no guteza imbere antibody. Mu myaka yashize, Alpha Lifetech yashyizeho uburyo bunoze bwo guteza imbere antibody (harimo iterambere rya antibody ya monoclonal, iterambere rya antibody ya polyclone, iterambere rya antibody ya recombinant monoclonal, hamwe nizindi serivisi zitegura antibody na serivisi ziterambere).

Alpha Lifetech iha abakiriya antibodi nziza na recombinant antibody na serivisi, kandi antibodies zateguwe zifite umwihariko kandi zihamye. Ibikoresho byinshi byogusukura antibody nibikoresho birashobora gukoreshwa mugutanga serivise zo kweza antibody ziva ahantu hatandukanye nkurukwavu, intama, inkoko, antibodiyite yimbeba, hamwe na serivise zo kweza poroteyine A / G hamwe na serivisi yo gutandukanya antibody.

Bishingiye ku buhanga butandukanye bwo gutegura antibody nka tekinoroji ya Hybridoma, Alpha Lifetech irashobora gutanga serivisi yihariye kuri antibodiyite za monoclonal na polyclone zo mu bwoko butandukanye. Byongeye kandi, serivisi zitandukanye kuva murwego rwo hejuru rwinjiza, gutegura antibody, kweza antibody kugeza antibody yemewe nabyo birashobora gutangwa. Tuzahitamo uburyo bwo kweza dushingiye kumigambi yihariye yabakiriya. Hashingiwe kuri sisitemu yuzuye nka porogaramu yo guteza imbere antibody hamwe na poroteyine ya poroteyine, Alpha Lifetech ikubiyemo serivisi zo hejuru no munsi y’ibikorwa byo gukora antibody, itanga isuku ya antibody, antibody ikwirakwizwa ryinshi, kwemeza antibody nizindi serivisi za tekiniki.

Ikoranabuhanga rya Hybridoma

Ikoranabuhanga rya Hybridoma rirashobora gukoreshwa mugutegura antibodiyite za monoclonal. Muguhuza ingirabuzimafatizo ziva mu mbeba zakingiwe hamwe na selile ya myeloma kuva ku bantu cyangwa ku mbeba, ingirabuzimafatizo za Hybridoma zirakorwa kandi zigasohora antibodi zihariye. Imbeba yakozwe ikomoka kuri antibodiyite ya monoclonal irashobora gukoreshwa mubushakashatsi bwa siyanse nka enzyme ihujwe na immunosorbent assay, radioimmunoassay, immunohistochemie, flux cytometrie, kweza poroteyine, nibindi. Ingirabuzimafatizo ya Hybridoma yatewe mu nda y’inyamaswa zirashobora kwiyongera no gusohora antibodiyite za monoclonal, zishobora kwirundanyiriza muri asite. Bitewe nibishoboka byanduye (proteine zinyamanswa, imisemburo, nibindi) muri izo antibodies, birasabwa kwezwa. Ukurikije imikorere yo gusohora ya lymphocytes B, antibodiyite za monoclonal akenshi zisohoka mumuco mugihe zihinga ingirabuzimafatizo. Amazi arimo antibodiyite za monoclonal murwego rwumuco yitwa hybridoma supernatant. Ubuziranenge bwa antibodiyite za monoclonal muri supernatant ni ndende cyane, ariko birasabwa kurushaho kwezwa kugirango hategurwe byinshi kuko biboneka binyuze mumico ya vitro.

Isuku ya Antibody irashobora gutandukanya antibodiyite zifite isuku nyinshi. Kugeza ubu, antibody affinity yoza no gutandukanya antibody no kweza bikoreshwa muburyo bwo kweza antibody, cyane cyane harimo kweza poroteyine A / G, gutunganya chromatografi ya ion, kuyungurura gel, kugwa hamwe na hydrophobique imikoranire ya chromatografiya. Muri byo, poroteyine A / G yo kweza serivisi irazwi cyane mubikorwa bitandukanye byo kweza antibody. Inzira ya Alpha Lifetech itegura antibodiyite za monoclonal zishingiye ku ikoranabuhanga rya hybridoma ryerekanwe ku gishushanyo 1.

Igishushanyo.1 Igishushanyo mbonera cyo gutegura antibodiyite zishingiye kuri tekinoroji ya hybridoma.

Antibody Ibiyobyabwenge hamwe na tekinoroji ya Hybridoma

Antibody ibiyobyabwenge conjugate (ADC) nigikoresho gishya cyibinyabuzima gihuza imiti ihuza antibodi nibiyobyabwenge bya cytotoxic. Antibody ibiyobyabwenge conjugate ikomatanya ubushobozi bwihariye bwo kumenya antibodi ningaruka zikomeye zo kwica imiti ya cytotoxic, kandi ikanatanga rwose imiti ya cytotoxique mumyanya mitsi ikoresheje antibodi, bityo ikavurwa neza. Igice cya antibody mumiti ya ADC gikunze gutegurwa hifashishijwe ikoranabuhanga rya hybridoma cyangwa ubundi buryo bwo gukora antibody, nka tekinoroji ya antibody yubuhanga. Ikoranabuhanga rya Hybridoma ritanga antibody nziza yo mu rwego rwo hejuru imiti ya ADC. Imiti ya ADC na antibodiyite za monoclonal zateguwe hakoreshejwe ikoranabuhanga rya hybridoma zigira uruhare runini mubice nko kuvura kanseri. Barashobora gukoreshwa bonyine cyangwa muguhuza kugirango bongere imbaraga zo kuvura.

Antibodiyite za Monoclonal zateguwe na tekinoroji ya hybridoma zifite umwihariko wo hejuru, zirashobora guhuza neza no kumenya antigene zihariye, kandi zishobora gukorwa ku rugero runini. Binyuze mu buhanga bwa tekinoroji ya Hybridoma, genes ya antibody mu ngirabuzimafatizo ya Hybridoma irashobora gukururwa no gukurikiranwa kugira ngo ibone uko bikurikirana, bigira akamaro mu mvugo ya antibody ikurikira. Antibodiyite za Monoclonal zateguwe hakoreshejwe ikoranabuhanga rya hybridoma zirashobora gukoreshwa mugutahura ibimenyetso byihariye byindwara, nkibimenyetso byibibyimba, antigene za virusi, nibindi, bitanga ubufasha bukomeye bwo gusuzuma indwara hakiri kare. Mubyongeyeho, irashobora kandi gukoreshwa mubigeragezo nka immunoblotting na immunohistochemie kugirango yige imvugo ya poroteyine, aho iherereye, imiterere, n'imikorere.

tekinoroji ya Hybridoma Umusaruro Antibody Service Workflow

Intambwe	Ibirimo	Igihe ntarengwa
Intambwe ya 1: Gukingira inyamaswa	(1) Gukingira inyamaswa inshuro 4, gukingira kwa booster inshuro 1, zose hamwe 5 . . Niba bidahuye n'ibisabwa, gukingira bisanzwe bizakomeza. (4) Imbaraga zujuje ibyangombwa, Utugingo ngengabuzima twitaruye kugirango duhuze selile.	Ibyumweru 10-12
Intambwe ya 2: Guhuza no Kugaragaza	. .	Ibyumweru 6
Intambwe ya 3: Subclone	Kwiyandikisha 15-25 ingirabuzimafatizo nziza ukoresheje uburyo buke bwo guhindagurika, hanyuma ukamenya clone 10-15 nziza	Ibyumweru 3-4
Intambwe ya 4: Asitike Umusaruro no Kweza Antibody	. . (3) Kweza poroteyine A / G: Kweza Asite	Ibyumweru 3-4
Intambwe ya 5: Gutanga	(1) Tanga 3-5 monoclonal antibody hybridoma. (2) Buri selile ya hybridoma itanga 1mg ya antibody isukuye (ascites kweza). (3) Raporo yubushakashatsi	Icyumweru 1

Ibyiza bya tekinoroji ya Hybridoma

Alpha Lifetech ifite uburambe bukomeye mubijyanye no guteza imbere antibody. Mu myaka yashize, Alpha Lifetech yashyizeho urubuga rwuzuye rwo guteza imbere antibody.

Ubwoko bwinshi

Ubudahangarwa bushobora gutangwa ku nyamaswa zitandukanye, harimo ariko ntizigarukira ku mbeba, inkwavu, intama, n'ibindi

Intego nyinshi

Ukurikije tekinoroji ya hybridoma, serivisi nyinshi zo kuvumbura antibody zirahari: proteyine, peptide, molekile nto, virusi, proteine membrane, mRNA, nibindi.

Inyandiko zikurikirana

QC ibipimo ngenderwaho byo kugenzura ubuziranenge (imbaraga z'umubiri, kugenzura ubuziranenge bwa PBMC, kugenzura ubuziranenge bw'isomero, no kugenzura ireme ry'ubuziranenge), raporo y'ubushakashatsi bw'icyongereza, inyandiko z'ubushakashatsi bw'umwimerere

Gahunda yihariye

Imwe kuri gahunda yihariye yihariye kugirango ihuze umushinga wubushakashatsi ukeneye kubakiriya batandukanye

UMURIMO Bifitanye isano

Ingamba nyinshi zo Gutezimbere Antibody

Serivisi ishinzwe iterambere rya Monoclonal

Ihuriro rya tekinoroji ya Hybridoma

Ihuriro rimwe rya selile

Icyiciro Cyerekana Antibody Iterambere

antibody ya monoclonal iterambere-Alpha Lifetech

Serivisi ishinzwe iterambere rya Monoclonal

Turashobora gutanga ubuziranenge bwo hejuru, bwera cyane, hamwe na serivise yihariye yo guteza imbere antibody ya monoclonal, harimo no gukora antibodiyite yimbeba na antibodiyite zinkwavu.