Urubuga rw'ikoranabuhanga rwa Hybridoma
Alpha Lifetech itanga serivisi nziza ku bakiriya ku bijyanye n’ingufu zirinda indwara ndetse n’ibicuruzwa na serivisi by’ingufu zirinda indwara ziterwa n’imiti.
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Urubuga rw'ikoranabuhanga rwa Hybridoma
Alpha Lifetech ifite ubunararibonye bwinshi mu bijyanye no guteza imbere uturemangingo tw’umubiri. Mu myaka yashize, Alpha Lifetech yashyizeho urubuga rusesuye rwo guteza imbere uturemangingo tw’umubiri (harimo guteza imbere uturemangingo tw’umubiri tw’ubwoko bwa monoclonal, guteza imbere uturemangingo tw’umubiri tw’ubwoko bwa polyclonal, guteza imbere uturemangingo tw’umubiri tw’ubwoko bwa monoclonal, hamwe n’izindi serivisi zo gutegura no guteza imbere uturemangingo tw’umubiri).
Alpha Lifetech itanga serivisi nziza ku bakiriya ku bijyanye n’ingufu zirwanya indwara ndetse n’izirwanya indwara zisubiramo, kandi izi ngufu zateguwe zifite ubushobozi bwo kwihutisha no kudahindagurika. Ibikoresho byinshi byo gusukura izi ngufu bishobora gukoreshwa mu gutanga serivisi zo gusukura izi ngufu ziturutse ahantu hatandukanye nko mu nkwavu, intama, inkoko, imbeba, ndetse na serivisi zo gusukura poroteyine A/G hamwe na serivisi zo gutandukanya izi ngufu.
Hashingiwe ku buryo butandukanye bwo gutegura antibody nka ikoranabuhanga rya hybridoma cell, Alpha Lifetech ishobora gutanga serivisi zihariye ku antibody za monoclonal na polyclonal zo mu bwoko butandukanye. Byongeye kandi, hari serivisi zitandukanye kuva ku gutondeka neza, gutegura antibody, gutunganya antibody kugeza ku kwemeza antibody. Tuzahitamo uburyo bwo gutunganya hashingiwe ku ntego yihariye y'umukiriya. Hashingiwe kuri sisitemu yuzuye nka platform development antibody na platform ya protein, Alpha Lifetech ikora serivisi zo hejuru n'eza nyuma zo gukora antibody, itanga serivisi zo gutunganya antibody, gutondeka neza antibody, kwemeza antibody n'izindi serivisi za tekiniki.
Ikoranabuhanga rya Hybridoma
Ikoranabuhanga rya Hybridoma rishobora gukoreshwa mu gutegura antibodies za monoclonal. Mu guhuza uturemangingo tw’umusemburo tuva ku mbeba zakingiwe hamwe n’uturemangingo twa myeloma tuva ku bantu cyangwa ku mbeba, uturemangingo twa hybridoma turakorwa kandi tugatanga antibodies zihariye. Antibodies za monoclonal zikomoka ku mbeba zishobora gukoreshwa mu bushakashatsi bwa siyansi nko gusuzuma immunosorbent assay, radioimmunoassay, immunohistochemistry, flow cytometry, protein purification, nibindi. Ingero za antibodies za monoclonal zirimo ascites, hybridoma supernatant, nibindi. Ingero zabonetse binyuze mu gutera uturemangingo twa hybridoma mu nda y’inyamaswa no gukoresha imiterere y’umubiri w’inyamaswa mu nda kugira ngo hakorwe antibodies zitwa ascites sample. Uturemangingo twa Hybridoma twinjijwe mu nda y’inyamaswa dushobora kwiyongera no gusohora antibodies za monoclonal, zishobora kwirunda muri ascites. Bitewe n’uko imyanda (poroteyine z’inyamaswa, imisemburo, nibindi) muri izi antibodies, ni ngombwa ko tuyungurura. Hashingiwe ku mikorere y’isohoka rya lymphocytes B, antibodies za monoclonal zikunze gusohoka mu buryo bwo gukurura iyo habayeho guhinga uturemangingo twa hybridoma. Amazi arimo antibodies monoclonal mu buryo bw’imiti yitwa hybridoma supernatant. Ubuziranenge bwa antibodies monoclonal muri supernatant buri hejuru, ariko hakenewe isuku yiyongereye kugira ngo hategurwe neza kuko biboneka binyuze mu miti ikorerwa muri vitro.
Gutunganya uturemangingo bishobora gutandukanya uturemangingo tw’umubiri dufite isuku nyinshi mu buryo bworoshye. Kuri ubu, uburyo bwo gutunganya uturemangingo tw’umubiri n’uburyo bwo gutandukanya uturemangingo tw’umubiri nibyo bikunze gukoreshwa mu gutunganya uturemangingo tw’umubiri, harimo cyane cyane protein A/G cleansing, ion exchange chromatography, gel filtration, precipitation na hydrophobic interaction chromatography. Muri byo, serivisi yo gutunganya uturemangingo tw’umubiri tw’umubiri tw’umubiri w’umubiri w’umubiri w’umubiri w’umuntu ikunzwe cyane mu buryo butandukanye bwo gutunganya uturemangingo tw’umubiri w’umuntu. Uburyo Alpha Lifetech itegura uturemangingo tw’umubiri tw’umuntu ...
Ishusho ya 1 Imbonerahamwe y'ibikorwa byo gutegura uturemangingo tw'umubiri dushingiye ku ikoranabuhanga rya hybridoma.
Ikoranabuhanga rya Antibody Drug Conjugate na Hybridoma
Antibody drug conjugate (ADC) ni ikintu gishya cya biological gihuza antibody na cytotoxic medications. Antibody drug conjugate ihuza ubushobozi bwihariye bwo kumenya antibody hamwe n'ingaruka zikomeye zo kwica imiti ya cytotoxic, kandi igatanga neza imiti ya cytotoxic mu turemangingo twa kanseri binyuze muri antibody, bityo igatanga ubuvuzi bunoze. Igice cy'antibody mu miti ya ADC gikunze gutegurwa binyuze mu ikoranabuhanga rya hybridoma cyangwa ubundi buryo bwo gukora antibody, nka ikoranabuhanga rya genetiki ya antibody. Ikoranabuhanga rya Hybridoma ritanga ibikoresho fatizo bya antibody byiza ku miti ya ADC. Imiti ya ADC na antibody za monoclonal zateguwe binyuze mu ikoranabuhanga rya hybridoma zigira uruhare runini mu nzego nko kuvura kanseri. Zishobora gukoreshwa zonyine cyangwa hamwe kugira ngo zongere imikorere myiza mu kuvura kanseri.
Antibodies za monoclonal zateguwe n'ikoranabuhanga rya hybridoma zifite umwihariko wo hejuru, zishobora gufata neza no kumenya antigens runaka, kandi zishobora gukorwa ku rugero runini. Binyuze mu ikoranabuhanga ryo gukurikirana hybridoma, genes za antibodies mu turemangingo twa hybridoma zishobora gukurwamo no gutondekanwa kugira ngo haboneke urukurikirane rujyanye na zo, ibyo bikaba ingirakamaro mu kugaragaza antibodies nyuma yaho. Antibodies za monoclonal zateguwe binyuze mu ikoranabuhanga rya hybridoma zishobora gukoreshwa mu kumenya ibimenyetso by'indwara runaka, nk'ibimenyetso bya kanseri, antigens za virusi, nibindi, bitanga ubufasha bukomeye mu gusuzuma indwara hakiri kare. Byongeye kandi, ishobora no gukoreshwa mu bushakashatsi nko gufunga immunoblotting na immunohistochemistry kugira ngo yige poroteyine igaragara, aho iherereye, imiterere, n'imikorere.
Ikoranabuhanga rya hybridoma mu gukora Antibody Service Inzira y'akazi
| Intambwe | Ibikubiye muri serivisi | Ingengabihe |
|---|---|---|
| Intambwe ya 1: Gukingira amatungo | (1) Gukingira amatungo inshuro 4, gukingira amatungo inshuro 1, gukingira amatungo inshuro 5 zose. (2) Amaraso y’amaraso ataranduye yakusanyijwe mbere yo gukingirwa, maze ELISA ikorwa ku gipimo cya kane kugira ngo hamenyekane titer y’amaraso mu maraso. (3) Niba titeri y'uturemangingo tw'amaraso y'umuti wa kane wujuje ibisabwa, indi dose imwe y'inkingo izatangwa iminsi 7 mbere yo gufata amaraso. Iyo itujuje ibisabwa, inkingo zisanzwe zizakomeza. (4) Ingufu zihagije, uturemangingo tw’imyakura turakurwaho kugira ngo uturemangingo twinjire. | Ibyumweru 10-12 |
| Intambwe ya 2: Guhuza no Gusuzuma | (1) Guhuza: Nyuma yo gukingirwa, uturemangingo tw’uduheri tw’imbeba twakusanyijwe tugahuzwa n’uturemangingo twa myeloma. (2) Kwiyongera no gupimwa: Uturemangingo twa hybridoma twabonetse twakorewe ikwirakwizwa ry’uturemangingo no gupimwa ELISA kugira ngo haboneke uturemangingo twiza cyane. | Ibyumweru 6 |
| Intambwe ya 3: Subclone | Gushyira mu kabumbe k'uturemangingo 15-25 twanduye hakoreshejwe uburyo bwo gutandukanya imiterere y'uturemangingo, hanyuma hagafatwa imiterere 10-15 yanduye | Ibyumweru 3-4 |
| Intambwe ya 4: Gukora Ascites no Gutunganya Antibody | (1) Gukora Ascites: Ubwoko 10-15 bwa hybridoma bukoreshwa mu gukora ascites mu mbeba (niba habonetse ubwoko bwinshi bwa hybridoma, buzoherezwa mu buryo bw'uturemangingo) (2) Kumenya Ascites: Gupima Ascites ELISA, imbaraga (poroteyine/virusi>10 ^ 5, molekile nto/peptide>10 ^ 4) zo gusukura igice cyo hasi (3) Gusukura Poroteyine A/G: Gusukura Ascites | Ibyumweru 3-4 |
| Intambwe ya 5: Gutanga | (1) Tanga ubwoko 3-5 bw'abasirikari b'umubiri ba monoclonal hybridoma. (2) Buri selile ya hybridoma itanga 1mg ya antibody isukuye (ascites cleansing). (3) Raporo y'Inzira y'Igerageza | Icyumweru kimwe |
Ubwoko bwinshi
Ubudahangarwa bw'umubiri bushobora gutangwa ku matungo atandukanye, harimo imbeba, inkwavu, intama, n'ibindi ariko ntibigarukira aho.
Intego nyinshi
Hashingiwe ku ikoranabuhanga rya hybridoma, serivisi nyinshi zo kuvumbura antibody zihari: poroteyine, peptides, molekile nto, virusi, poroteyine zo mu ruhu, mRNA, nibindi.
Inyandiko zo gukurikirana
Ibipimo ngenderwaho byo kugenzura ubuziranenge bwa QC (imbaraga z'ubudahangarwa bw'umubiri, kugenzura ubuziranenge bwa PBMC, kugenzura ubuziranenge bw'isomero, no kugenzura ubuziranenge bw'isuzuma), raporo z'igerageza ry'Icyongereza, inyandiko z'igerageza z'umwimerere
Porogaramu yihariye
Guhindura gahunda y'umuntu ku giti cye kugira ngo ihuze n'ibyo abakiriya batandukanye bakeneye mu bushakashatsi
Serivisi yo guteza imbere ubudahangarwa bw'umubiri bwa monoclonal
Dushobora gutanga serivisi zo guteza imbere antibody za monoclonal nziza, zisukuye cyane, kandi zihariye, harimo gukora antibody za monoclonal z'imbeba n'abasirikari b'inkwavu ba monoclonal.
Urubuga rw'ikoranabuhanga rwa Hybridoma
Harimo gahunda yo gukingira, serivisi zo gutegura antibody, gutunganya antibody, gukurikirana antibody nyinshi, kwemeza antibody, nibindi
Urubuga rwo Gutondekanya Seli Imwe ya B
Alpha Lifetech ifite ibyiza mu gihe cyo gusuzuma no kubona antibodies nziza. Ishobora gutanga imiterere ya antigen, imikorere, no guhindura, ubudahangarwa bw'inyamaswa, gusuzuma ubwiyongere bw'uturemangingo twa B imwe, no gukurikirana uturemangingo tubiri.
Urubuga rwo guteza imbere ubudahangarwa bw'umubiri mu kwerekana ibimenyetso bya phage
Alpha Lifetech ishobora gutanga serivisi za tekiniki zo guteza imbere antibody zo kwerekana phage, kuva mu gutegura antibody, gutunganya antibody, gukurikirana antibody, nibindi.
Ikoranabuhanga rijyanye naryo
Intangiriro ku ikoranabuhanga rya HybridomaVIDEWO
Niba ufite ikibazo icyo ari cyo cyose, twandikire igihe icyo ari cyo cyose.
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2018-07-16 
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