Urubuga rwo guteza imbere ubudahangarwa bw'umubiri mu kwerekana ibimenyetso bya phage
Hashingiwe ku buryo bwuzuye bwo kubaka urubuga rw’abarwanyi, Alpha Lifetech ishobora gutanga serivisi za tekiniki kuva ku gutegura abarwanyi, gutunganya abarwanyi, gukurikirana abarwanyi, nibindi.
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Urubuga rwo guteza imbere ubudahangarwa bw'umubiri mu kwerekana ibimenyetso bya phage
Alpha Lifetech, ifite uburambe bwinshi mu mishinga yo kuvumbura antibody, ishobora gutanga serivisi zihariye zo kurwanya antibody za monoclonal na polyclonal ku moko menshi, ndetse no kubaka isomero ry’abarwayi no gusuzuma antibody. Alpha Lifetech ishobora guha abakiriya ibikoresho by’abarwayi birinda indwara n’ibikomoka ku barwayi bivanze neza, ndetse na serivisi zijyanye nabyo, kugira ngo bategure antibody nziza, zihariye kandi zihamye. Dukoresheje urubuga rwagutse rw’abarwayi n’ubuhanga bwo kurwanya antibody, dutanga serivisi zijyanye no gukora antibody mu buryo bw’ibanze no mu buryo bw’ibanze, harimo serivisi za tekiniki nko gutegura antibody, gutunganya, gukurikirana antibody, no kwemeza.
Alpha Lifetech ifite urubuga rwo kuvumbura uturemangingo tw’umubiri rukuze rutanga serivisi zitandukanye zirimo ubudahangarwa bw’inyamaswa, kubaka no gusuzuma isomero ry’uturemangingo tw’uturemangingo tw’udukoko twa phage, gukurikirana uturemangingo tw’uturemangingo, kugaragaza uturemangingo tw’umubiri, gutunganya uturemangingo tw’umubiri, kwemeza uturemangingo tw’umubiri, no gushyiraho ibirango by’uturemangingo tw’umubiri bishingiye ku ikoranabuhanga rya hybridoma, ikoranabuhanga rya selile imwe B, ikoranabuhanga ryo kwerekana uturemangingo tw’umubiri twa phage, n’ibindi. Alpha Lifetech ifite umurongo ngenderwaho w’imikorere, kandi uretse serivisi zo gutegura uturemangingo tw’umubiri, serivisi zishyigikira nko gukwirakwiza uturemangingo tw’umubiri, gukura kwa uturemangingo tw’umubiri, gushushanya no guteza imbere imiti ya ADC, ndetse no gushushanya imiterere y’uruhererekane rwa CAR-T nabyo birashobora gutangwa. Muri icyo gihe, Alpha Lifetech yubatse isomero ry’uturemangingo tw’uturemangingo tw’uturemangingo tw’uturemangingo tw’umubiri dushingiye kuri platforms za M13, T4, na T7 phage, rifite ubushobozi bwo kubika kugeza kuri 10^8-10^9. Igipimo cyiza, igipimo cyo gushyiramo, n’ubusumbane bw’isomero byose bishobora kugera kuri 90%.
Ikoranabuhanga ryo kwerekana phage
Ikorwa ry’uturemangingo tw’umubiri twitwa monoclonal ryabanje gutegurwa hakoreshejwe ikoranabuhanga rya hybridoma monoclonal antibody kugira ngo hakorwe antibody y’umubiri witwa hybridoma monoclonal mu mbeba. By’umwihariko, binyuze mu guhuza uturemangingo tw’umugongo tuva mu mbeba zakingiwe hamwe n’uturemangingo twa myeloma tuva ku bantu cyangwa ku mbeba, hakorwa uturemangingo twa hybridoma, dusohora uturemangingo tw’umubiri twitwa hybridoma monoclonal antibody. Mu guhindura uturemangingo tw’umubiri twitwa monoclonal mu mbeba hakoreshejwe imiterere y’umubiri no guhindura uturemangingo tw’umubiri kugira ngo duhabwe agace gahoraho k’ubudahangarwa bw’umubiri bw’umuntu, ubushobozi bwatwo bwo kwirinda indwara bushobora kugabanuka. Ubuhanga bwo kurwanya indwara zitwa monoclonal bukoreshwa cyane mu buvuzi no mu buvuzi.
Alpha Lifetech ishobora gutanga serivisi zo gukora ubwubatsi bw’uturemangingo tw’umubiri kuva ku gushushanya protocole y’ubushakashatsi mbere y’uko abaganga batangira kuvumbura imiti ihuza uturemangingo (ADCs) no kwemeza inyamaswa. Uturemangingo tw’uturemangingo tw’umubiri (ADCs) dushobora gutanga imiti ya chimiotherapie ku turemangingo twa kanseri. Nyuma yo kwihuza n’ibintu byihariye bigaragara ku turemangingo twa kanseri, ADC isohora imiti ihumanya ikirere mu turemangingo twa kanseri. Alpha Lifetech ishobora kandi guha abakiriya serivisi zuzuye zo gukura kw’uturemangingo tw’umubiri. Hamwe n’ikoranabuhanga rigezweho ryo gukora neza mu mihindukire y’umubiri n’ikoranabuhanga ryo gusuzuma ibimenyetso bya phage, uturemangingo tw’umubiri dufite aside runaka turabanza gusuzumwa, hanyuma hagashyirwaho aside amine kugira ngo haboneke ubwoko butandukanye. Nyuma yaho, uturemangingo tw’umubiri dufite aside nyinshi twarasuzumwe kandi tugasuzumwa hakoreshejwe uburyo bwo gusuzuma. Nyuma y’ibyiciro byinshi byo gukora neza no gusesengura imiterere y’umubiri, uturemangingo tw’umubiri dufite aside nyinshi kandi dufite umwihariko ukomeye amaherezo twabonetse.
Alpha Lifetech ishobora kubaka ubwoko butandukanye bw'amasomero yerekana antibody phage, harimo amasomero y'ubudahangarwa bw'umubiri, amasomero gakondo, amasomero y'ubudahangarwa bw'igice, n'amasomero y'ubudahangarwa bw'umubiri. Hashingiwe ku bushobozi bwinshi bw'amasomero ya antibody, antibody yihariye cyane irashobora kuboneka. Vectors nyinshi za phagemid nka pMECS, pComb3X, na pCANTAB 5E zishobora gutangwa, ndetse n'ubwoko nka TG1 E. coli, XL1 Blue, na ER2738. Uretse ubushobozi bw'isomero bugera kuri 10^9, igipimo cyo gushyiramo ibice by'isomero nacyo kiri hejuru, bigatuma habaho uburyo buhagije bwo gusuzuma antibody zishimisha abakiriya. Imbonerahamwe y'ibikorwa bya serivisi yo gutegura antibody ya Alpha Lifetech ishingiye ku ikoranabuhanga ryo kwerekana phage igaragara ku Ishusho ya 1.
Igishushanyo cya 1 Imbonerahamwe y'ibikorwa byo gutegura uturemangingo tw'umubiri dushingiye ku ikoranabuhanga ryo kwerekana phage.
Imikorere y'imikorere y'imashini zirwanya indwara zo mu bwoko bwa Phage Display
| Intambwe | Ibikubiye muri serivisi | Ingengabihe |
|---|---|---|
| Intambwe ya 1: Gukingira amatungo | (1) Gukingira amatungo inshuro 4, gukingira amatungo inshuro 1, gukingira amatungo inshuro 5 zose. (2) Seramu itari nziza mbere yo gukingirwa yakusanyijwe, maze ELISA ikorwa ku gipimo cya kane kugira ngo hamenyekane titer y'amaraso mu maraso. (3) Niba titeri y'uturemangingo tw'amaraso y'umuti wa kane wujuje ibisabwa, indi dose imwe y'inkingo izatangwa iminsi 7 mbere yo gufata amaraso. Iyo itujuje ibisabwa, inkingo zisanzwe zizakomeza. (4) Ingufu zikwiye, gukusanya amaraso no kuyatandukanya n'amasasu ya monocytes | Ibyumweru 10 |
| Intambwe ya 2: Gutegura cDNA | (1) PBMC Total RNA Extraction (Kit yo Gukuramo RNA) (2) Gutegura cDNA (reverse transcription kit) hifashishijwe RT-PCR (uburyo bworoshye bwo kuyitegura) | Umunsi umwe |
| Intambwe ya 3: Kubaka Isomero rya Antibody | (1) Hakoreshejwe cDNA nk'icyitegererezo, genes zongerewe imbaraga hakoreshejwe PCR ebyiri. (2) Imiterere n'impinduka za phage: vektori ya phagemid ihuza gene, impinduka za bagiteri za TG1 zikoresha electroporation, iyubakwa ry'isomero ry'abarwanya indwara. (3) Kumenya: Hitamo ubwoko 24 bwa clones mu buryo butaziguye, PCR igaragaza igipimo cya positive + igipimo cyo gushyiramo. (4) Gutegura fagi hifashishijwe uburyo bwo kuyitunganya: kongera ingufu za fagi za M13+gutunganya. (5) Ubutabazi bw'isomero ryerekana imiterere ya Phage | Ibyumweru 3-4 |
| Intambwe ya 4: Gusuzuma isomero ry’abarwanyi (inshuro 3) | (1) Gusuzuma inshuro eshatu (gupima igice gikomeye): gusuzuma igitutu kugira ngo ukureho ubudahangarwa bw’umubiri butari bwihariye ku rugero rushoboka rwose. (2) Bacteriophage imwe yatoranijwe mu kongera ubushobozi bwa kloni imwe+IPTG iterwa no kugaragaza neza kloni imwe+ELISA. (3) Imiterere yose myiza yatoranijwe kugira ngo hakorwe urutonde rw'uturemangingo. | Ibyumweru 4-5 |
Serivisi z'ubufasha
Dushobora gutanga serivisi zitandukanye zo kubaka isomero ry’umubiri rishingiye ku matungo ndetse na serivisi zo gusuzuma ubudahangarwa bw’umubiri bushingiye ku matungo hakurikijwe ibyo abakiriya bakeneye.
Intego nyinshi
Serivisi nyinshi zo kuvumbura antibody zihari: poroteyine, peptides, molekile nto, virusi, poroteyine zo mu ruhu, mRNA, nibindi.
Vekteri nyinshi
Serivisi y’ubwubatsi bw’isomero ryihariye, dushobora gutanga vekteriyofagi zitandukanye zirimo PMECS, pComb3X, na pCANTAB 5E, no kuzihindura hakurikijwe ibyo abakiriya bakeneye.
Urubuga rw'abantu bakuru
Ubushobozi bwo kubika bushobora kugera kuri 10 ^ 8-10 ^ 9, igipimo cyo gushyiramo kiri hejuru ya 90%, kandi ubwinshi bw'uturemangingo tw'umubiri twabonetse binyuze mu gusuzuma muri rusange buri ku rwego rwa nM pM.
Serivisi yo guteza imbere ubudahangarwa bw'umubiri bwa monoclonal
Dushobora gutanga serivisi zo guteza imbere antibody za monoclonal nziza, zisukuye cyane, kandi zihariye, harimo gukora antibody za monoclonal z'imbeba n'abasirikari b'inkwavu ba monoclonal.
Urubuga rw'ikoranabuhanga rwa Hybridoma
Harimo gahunda yo gukingira, serivisi zo gutegura antibody, gutunganya antibody, gukurikirana antibody nyinshi, kwemeza antibody, nibindi
Urubuga rwo Gutondekanya Seli Imwe ya B
Alpha Lifetech ifite ibyiza mu gihe cyo gusuzuma no kubona antibodies nziza. Ishobora gutanga imiterere ya antigen, imikorere, no guhindura, ubudahangarwa bw'inyamaswa, gusuzuma ubwiyongere bw'uturemangingo twa B imwe, no gukurikirana uturemangingo tubiri.
Urubuga rwo guteza imbere ubudahangarwa bw'umubiri mu kwerekana ibimenyetso bya phage
Alpha Lifetech ishobora gutanga serivisi za tekiniki zo guteza imbere antibody zo kwerekana phage, kuva mu gutegura antibody, gutunganya antibody, gukurikirana antibody, nibindi.
Niba ufite ikibazo icyo ari cyo cyose, twandikire igihe icyo ari cyo cyose.
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2018-07-16 
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