Icyiciro Cyerekana Antibody Iterambere

Alpha Lifetech, hamwe nuburambe bunini bwumushinga muguvumbura antibody, irashobora gutanga serivisi yihariye ya antibody ya monoclonal na polyclone kubinyabuzima byinshi, ndetse no kubaka isomero rya antibody na serivisi zo gusuzuma. Alpha Lifetech irashobora guha abakiriya antibody nziza kandi yizewe ya antibody ya recombinant, hamwe na serivisi zijyanye, kugirango bategure antibodi nziza, zihariye, kandi zihamye. Mugukoresha uburyo bwuzuye bwa antibody hamwe na antibody yubuhanga, dutanga serivise zikubiyemo epfo na ruguru yumusaruro wa antibody, harimo serivisi tekinike nko gutegura antibody, kweza, gukurikirana antibody, no kwemeza.

Alpha Lifetech ifite urubuga rwo kuvumbura antibody rukuze ruha abakiriya serivisi zitandukanye zirimo ubudahangarwa bw’inyamaswa, icyiciro cya fagitire yerekana isomero ry’isomero rya antibody no gusuzuma, ikurikiranwa rya antibody, imvugo ya antibody, kweza antibody, kwemeza antibody, hamwe na label yerekana antibody ishingiye ku ikorana buhanga rya Hybridoma, ikoranabuhanga rya selile B, ikoranabuhanga rya fage, n'ibindi. Alpha Lifetech ifite umurongo ngenderwaho usanzwe, kandi usibye serivisi zitegura antibody, serivisi zifasha nka antibody muntu, gukura kwa antibody, gukura kwa ADC no guteza imbere, hamwe na CAR-T ikurikiranye ikurikiranye irashobora gutangwa. Muri icyo gihe, Alpha Lifetech yubatse isomero rya antibody rishingiye ku mbuga za tekinoroji ya M13, T4, na T7, ifite ububiko bugera kuri 10 ^ 8-10 ^ 9. Igipimo cyiza, igipimo cyo kwinjiza, nuburyo butandukanye bwibitabo bushobora kugera kuri 90%.

Icyiciro cyerekana Ikoranabuhanga

Umusemburo wa antibodiyumu wa Monoclonal wateguwe bwa mbere ukoresheje tekinoroji ya antibody ya hybridoma monoclonal kugirango ubyare imbeba ya hybridoma monoclonal antibody. By'umwihariko, muguhuza ingirabuzimafatizo ziva mu mbeba zakingiwe hamwe na selile ya myeloma ziva mu bantu cyangwa ku mbeba, ingirabuzimafatizo za Hybridoma ziba zikora antibodiyite zidasanzwe za hybridoma monoclonal antibody. Muguhindura abantu muburyo bwa antibodiyite yimbeba no guhindura ingengabihe ya antibodiyite kugirango ibahe akarere ka immunoglobuline gahoraho, ubudahangarwa bwabo burashobora kugabanuka. Ubwubatsi bwa antibody ya monoclonal bukoreshwa cyane mubikorwa bya biomedical na clinique.

Alpha Lifetech irashobora gutanga serivisi imwe yubuhanga bwa antibody kuva mubushakashatsi bwibanze bwa protocole kugeza kubiyobyabwenge bya antibody (ADCs) kuvumbura no kwemeza inyamaswa. Imiti igabanya ubukana (ADCs) irashobora gutanga imiti ya chimiotherapie kanseri ya kanseri. Nyuma yo guhuza intego zihariye zigaragara ku ngirabuzimafatizo za kanseri, ADC irekura imiti ya cytotoxique mu ngirabuzimafatizo za kanseri. Alpha Lifetech irashobora kandi guha abakiriya serivisi zuzuye zo gukura kwa antibody. Hamwe nogutezimbere kwiterambere rya mutation hamwe no kwinjiza byinshi murwego rwo kwerekana tekinoroji, antibodies zifite isano runaka zabanje gusuzumwa, hanyuma ihinduka rya aside amine ryinjizwa kugirango ritange ibintu bitandukanye. Icyakurikiyeho, antibodies nyinshi zifatika zarasuzumwe kandi zirasuzumwa hakoreshejwe tekinoroji yo gusuzuma. Nyuma yuburyo bwinshi bwo gukora neza no gusesengura imiterere, antibodies zifite isano iri hejuru kandi zihariye zabonetse.

Alpha Lifetech irashobora kubaka ubwoko butandukanye bwa antibody fage yerekana amasomero, harimo amasomero yumubiri, amasomero kavukire, amasomero yubukorikori, hamwe nububiko bwibitabo. Ukurikije ubushobozi bwinshi bwamasomero ya antibody, antibodies zihariye zirashobora kuboneka. Ibice byinshi bya fagemide nka pMECS, pComb3X, na pCANTAB 5E birashobora gutangwa, hamwe nubwoko nka TG1 E. coli, XL1 Ubururu, na ER2738. Usibye ubushobozi bw'isomero bugera kuri 10 ^ 9, igipimo cyo kwinjiza ibice by'isomero nacyo kiri hejuru, bituma habaho uburyo buhagije bwo gusuzuma antibodi zishimisha abakiriya. Igishushanyo mbonera cya serivisi yo gutegura antibody ya Alpha Lifetech ishingiye ku buhanga bwo kwerekana fage irerekanwa mu gishushanyo 1.

Igishushanyo.1 Igishushanyo mbonera cyo gutegura antibodiyite za monoclonal zishingiye ku buhanga bwo kwerekana fage.

Icyiciro Cyerekana Antibody Umusaruro Wakazi

Intambwe	Ibirimo	Igihe ntarengwa
Intambwe ya 1: Gukingira inyamaswa	(1) Gukingira inyamaswa inshuro 4, gukingira kwa booster inshuro 1, zose hamwe 5 . . Niba bidahuye n'ibisabwa, gukingira bisanzwe bizakomeza. (4) Imbaraga zujuje ibisabwa, gukusanya amaraso no gutandukanya monocytes	Ibyumweru 10
Intambwe ya 2: Gutegura cDNA	(1) PBMC Gukuramo RNA yose (Igikoresho cyo gukuramo RNA) .	Umunsi 1
Intambwe ya 3: Kubaka Isomero rya Antibody	(1) Ukoresheje cDNA nkicyitegererezo, genes zongerewe nibice bibiri bya PCR. . (3) Kumenyekanisha: Hitamo neza clone 24, PCR iranga igipimo cyiza + igipimo cyo kwinjiza. (4) Gufasha gutegura fage: M13 icyiciro cya amplification + kweza. (5) Icyiciro cyo kwerekana isomero gutabara	Ibyumweru 3-4
Intambwe ya 4: Kwerekana Isomero rya Antibody (3 round)	. . (3) Clone zose nziza zatoranijwe kugirango zikurikirane gene.	Ibyumweru 4-5

Ibyiza bya fage Yerekana Ikoranabuhanga

Alpha Lifetech ifite uburambe bukomeye mubijyanye no guteza imbere antibody. Mu myaka yashize, Alpha Lifetech yashyizeho urubuga rwuzuye rwo guteza imbere antibody.

Serivisi zunganira

Turashobora gutanga serivisi zinyuranye zububiko bwibitabo byubudahangarwa hamwe na serivise zisanzwe zo gusuzuma isomero rya antibody dukurikije ibyo abakiriya bakeneye

Intego nyinshi

Serivisi nyinshi zo kuvumbura antibody zirahari: proteyine, peptide, molekile nto, virusi, proteine membrane, mRNA, nibindi.

Inzego nyinshi

Serivisi yo kubaka isomero yihariye, turashobora gutanga za bacteriofage zitandukanye zirimo PMECS, pComb3X, na pCANTAB 5E, hanyuma tukayihindura dukurikije ibyo abakiriya bakeneye.

Ihuriro rikuze

Ubushobozi bwo kubika bushobora kugera kuri 10 ^ 8-10 ^ 9, igipimo cyo kwinjiza kiri hejuru ya 90%, kandi isano ya antibodies zabonetse binyuze mugupima muri rusange kurwego rwa nM pM

UMURIMO Bifitanye isano

Ingamba nyinshi zo Gutezimbere Antibody

Serivisi ishinzwe iterambere rya Monoclonal

Ihuriro rya tekinoroji ya Hybridoma

Ihuriro rimwe rya selile

Icyiciro Cyerekana Antibody Iterambere

antibody ya monoclonal iterambere-Alpha Lifetech

Serivisi ishinzwe iterambere rya Monoclonal

Turashobora gutanga ubuziranenge bwo hejuru, bwera cyane, hamwe na serivise yihariye yo guteza imbere antibody ya monoclonal, harimo no gukora antibodiyite yimbeba na antibodiyite zinkwavu.

Ihuriro rya tekinoroji ya Hybridoma

Harimo gahunda yo gukingira, serivisi zo gutegura antibody, kweza antibody, antibody nyinshi zikurikirana, kwemeza antibody, nibindi

Ihuriro rimwe rya selile

Alpha Lifetech ifite ibyiza mugihe cyo gusuzuma no kubona antibodies nziza. Irashobora gutanga igishushanyo cya antigen, synthesis, no guhindura, ubudahangarwa bwinyamaswa, gusuzuma B selile imwe ikungahaye, ikurikiranye selile imwe.