Serivisi yo gusuzuma isomero rya Phage Display

Alpha Lifetech imaze imyaka myinshi ikora cyane mu ikoranabuhanga ryo kwerekana Phage. Yubatse urubuga rwiza rw'ikoranabuhanga ryo kwerekana Phage rutuma umuntu atakaza umwanya wo gukora ubushakashatsi bwa siyansi cyangwa ubushakashatsi ku mishinga, kandi rukorohereza umusaruro ukurikiraho. Alpha Lifetech ishobora kandi guha abakiriya serivisi nko gukora antibody ya vhh, gukora antibody ya scFv, gukora antibody ya Fab.

Alpha Lifetech ifite urubuga rwa M13/T7 phage rwuzuye rwo kwerekana no gukora amasomero y’abarwanya indwara. Dushobora kandi gutanga serivisi zihariye, nko gukora antibody scFv no gusuzuma antibody nyinshi, kugira ngo duhuze n’ibyo ukeneye.

Uburyo bwo kubaka isomero rya Phage Display

Uburyo bwo kubaka isomero rya phage bukurikira: primers zihariye zigenewe kongera imbaraga za PCR zigakuramo ibyo bicuruzwa, bihuzwa na enzyme-ligated na T7/M13 phage vector, maze plasmid ya phage recombinant ikorwa neza. Plasmid ya phage recombinant yahinduwemo uturemangingo twa TG1 receptor, hanyuma ishyirwa ku kintu kirimo antibiyotike zikwiye, maze culture yagutse ikorwa nyuma yo gusuzuma transformers. Nyuma yo gusesengura inshuro nyinshi, phage yiyongera inshuro zo gusesengura muri bagiteri kandi ikagaragaza neza poroteyine cyangwa polypeptide. Hanyuma isomero rya phage rirasukurwa kugira ngo rikureho imyanda imwe n'imwe na phage zifunguye.

Uburyo bwo gusuzuma isomero rya Phage Display

Intsinzi y'ikorwa ry'uturemangingo tw'umubiri dushingiye ahanini ku bubiko bw'uturemangingo tw'umubiri dushingiye ku bubiko bw'umubiri, kandi uburyo busanzwe bwo gupima uduce duto ni ugupima uduce duto n'uduce duto. Gupima uduce duto dukoresha imiti yunguka uduce twihariye binyuze mu gupima uduce duto duto 3-4 kugira ngo haboneke uturemangingo tw'umubiri dushingiye ku mibiri; Gupima uduce duto dukoresha imiti yunguka hakoreshejwe uburyo bwa biotin bugaragaza ko ari ngombwa kandi bugafata uduce duto dukoresheje uduce duto tw'ingufu. Gupima uduce duto dukoresha imiti yunguka cyane kandi uduce duto tw'umubiri dukoreshwa mu miti duhagarikwa. Gupima uduce duto dukoreshwa mu miti bigira ingaruka nziza kandi bikungahaye ku miterere y'uturemangingo, ariko uturemangingo twabonetse ntabwo twihariye. Uburyo bwo gupima uduce duto dushingiye ku bikenewe mu igerageza, byose bizagira ingaruka ku ngaruka zo gukora uduce duto tw'umubiri dushingiye ku mibiri.

Gupima Antibody Ifite Umuvuduko Ukabije

Iterambere ry’uturemangingo tw’umubiri ryarushijeho kwiyongera mu buvuzi bwa none. Hari uburyo butandukanye bwo guteza imbere uturemangingo tw’umubiri, ariko gusuzuma uturemangingo tw’umubiri twinshi bikoreshwa cyane mu buvuzi. Ubu buryo bushobora gutuma habaho ubwoko bwinshi bw’uturemangingo tw’umubiri. Guhuza n’uburyo gakondo bwo gusuzuma uturemangingo tw’umubiri bishobora kunoza ireme ry’amasomero yo kwerekana udukoko tw’umubiri. Nyuma yo gusuzuma ibinyabuzima, uturemangingo tw’umubiri dushobora kurangwa na ELISA yoroheje cyangwa gusuzuma uturemangingo tw’umubiri twinshi.

Ishusho ya 1 Imbonerahamwe igaragaza urukurikirane rw'ibyabaye kuva mu kubaka amasomero ya antibody ya phage kugeza ku ikorwa rya antibody za monoclonal zifite affinity nyinshi kuri antigens hamwe n'ubushobozi buke bwo kwirinda indwara.(Inkomoko y'aho uherereye: Ikoranabuhanga ryo kwerekana phage nk'urubuga rukomeye rwo kuvumbura imiti irwanya indwara - PMC (nih.gov))

Amabwiriza yo Kwitondera Kugaragaza Ubudahangarwa bw'Indwara z'UmubiriAmabwiriza yo Kwirinda

Ibitekerezo ku birebana n'ibice bito

Guhitamo ibyuma bifasha mu gupima imiterere y’ikintu kigamije gupima imiterere y’ikintu, imiterere y’ibidukikije bihuza, ndetse no gufata ibintu mu buryo butari bwihariye mu gihe gikoreshwa. Poroteyine zidafitanye isano, isabune zitari ionic, n’umunyu mwinshi mu mubiri ni byiza mu kugabanya inyuma. Hariho n’ibindi bintu, nko ko hari ibikenewe kongerwamo ibyuma bihuza imiterere y’ikintu cyangwa ibindi bifasha mu gupima imiterere y’ikintu, ibyuma bifasha mu gupima imiterere y’ikintu bishobora guhuzwa n’ikintu kigamije gupima imiterere y’ikintu, bishobora kongerwa ku gice gifasha mu gupima imiterere y’ikintu, ndetse bigakoreshwa no mu gufata ikintu kigamije gupima imiterere y’ikintu.

Ibitekerezo ku gitutu

Ibipimo bisanzwe by’umuvuduko w’ibipimo birimo ubwinshi bw’ibipimo bya molekile, igihe cyo gufatanya, imbaraga zo gukaraba, nibindi. Izindi nzira zo kongera umuvuduko w’ibipimo birimo gukoresha ibintu bidafite akamaro (nk'aside, urea, na guanidine), ibintu bisukura ibinyabuzima, kongera ubushyuhe, cyangwa kongera ubwinshi bw’ibipimo cyangwa intego bihanganye mu gikoresho cyo gukaraba.
Muri byo, icyiciro cya mbere cyo gusuzuma ni ingenzi. Icyiciro cya mbere cyo gusuzuma ni ugufata clones nyinshi zishoboka zivuye mu isomero ryubatswe mu gihe hakurwamo clones zitari izihariye. Hagomba gukoreshwa target zifite imyenge cyangwa umubare munini w'intwaro zishobora gushonga kugira ngo hafatwe umubare munini w'udusimba dufatanye kugira ngo isomero rikomeze kuba ryiza. Mu icyiciro cya nyuma cyo gusuzuma, igitutu cyo guhitamo kigomba kwiyongera uko ubwiyongere bw'ibicuruzwa bwiyongera, gukaraba inshuro nyinshi, no kongera igihe cyo gukaraba, clones zifite ubwinshi bushoboka zishobora gupimwa.

Ingamba zo Gusuzuma Ibibi

Ibimenyetso bya antigen n'ibikoresho biyifasha gusuzuma ni ugusuzuma ibintu byose biri ku matsinda ya antigen, nka tags, proteine zo guhuza, n'ibindi bintu biyifasha. Gusuzuma nabi ibintu bitari ibyafashwe bishobora kugabanya ubwiyongere bw'uturemangingo tutari antigen.
Uturemangingo twuzuye, liposome, disiki za nano-phospholipid, na VLPs bishobora gupimwa nabi hakoreshejwe uturemangingo twahinduwe cyangwa uturemangingo tudafite ubwiyongere mu gihe cyo gusuzuma imirongo y’uturemangingo twahinduwe.
Gukuraho uruvange rw’ama-antigen akomeye Gusuzuma neza ibitera indwara bishobora gukorwa ku ma-molecule atazwi cyangwa agoye, nk'uturemangingo twuzuye cyangwa poroteyine zitanduye.
Ingamba zo kurwanya abasirikari b’umubiri ku hantu runaka hagaragara abasirikari b’umubiri, imwe ni ukugabanya abasirikari b’umubiri bashobora guhangana na ligand bongeramo ligand nyinshi, indi ni ukubuza abasirikari b’umubiri.

Imikoreshereze ya Phage Display

*Guvumbura antibody byarushijeho kuba ingenzi mu buvuzi bwa none. Hari uburyo butandukanye bwo kuvumbura antibody, ariko ikoranabuhanga ryo kwerekana phage rikoreshwa cyane mu buvuzi. Kuva mu 1990, imiterere itandukanye y’antibody yakoreshejwe mu kubaka amasomero ya phage, harimo VH, VHH, scFvs, diabodies, na Fab antibodies.
*Amasomero ya peptide ya Phage yemerera kumenya vuba urukurikirane rwa epitopes za poroteyine kandi yabaye igikoresho gikomeye cyo gushakisha imikoranire iri hagati ya epitopes na antigen receptors.
*Ibice by’uturemangingo tw’umubiri bihuzwa na G3P ya phage ya M13, kandi binyuze mu guhuza umubare munini w’uturemangingo tw’umubiri dufata igice cy’uturemangingo tw’umubiri, hashobora gukorwa ibitabo binini by’uturemangingo tw’umubiri dufatanyirizwa hamwe, aho uturemangingo twinshi tw’uturemangingo dushobora gutoranywamo.
*Sisitemu yo kwerekana T7 phage ifite ibyiza byinshi, birimo koroshya, umutekano mwinshi, ituze, kubika byoroshye, no gutwara, bityo iyi sisitemu ikoreshwa mu nkingo zo kwirinda no kuvura.
*Sisitemu yo kwerekana T7 phage ishobora kubona antigen zitandukanye, nka antigen zo hejuru z'udukoko dutera indwara na antigen za kanseri.

Phage