Icyiciro cyo kwerekana Isomero rya Serivisi

Alpha Lifetech amaze imyaka myinshi akora cyane mubuhanga bwo kwerekana Phage. Yubatse urubuga rwiza rwa tekinoroji ya Phage yerekana ikora neza kubushakashatsi bwubumenyi cyangwa ubushakashatsi bwumushinga kandi byoroshya umusaruro. Alpha Lifetech irashobora kandi guha abakiriya serivisi nka vhh antibody, umusaruro wa scFv antibody, umusaruro wa Fab Antibody.

Alpha Lifetech ifite urubuga rwuzuye rwa M13 / T7 rwo kwerekana ibyubaka no gutanga amasomero ya antibody. Turashobora kandi gutanga serivise yihariye, nkumusemburo wa antibody scFv hamwe no kugenzura antibody nyinshi, kugirango uhuze ibyo ukeneye.

Icyiciro cyerekana Isomero ryuburyo bwo kubaka

Igikorwa cyo kubaka isomero rya fage nuburyo bukurikira: primers yihariye yagenewe amplification ya PCR ibona ibicuruzwa, bikaba enzyme ihujwe na vector ya T7 / M13, kandi recombinant phage plasmid yubatswe neza. Fomb plasmid ya recombinant yahinduwe ingirabuzimafatizo za TG1, hanyuma igashyirwa ku gikoresho kirimo antibiyotike ikwiye, kandi umuco wagutse wakozwe nyuma yo gusuzuma transformateur. Nyuma yimico myinshi yumuco, fage yigana ibihe byo kwigana muri bagiteri kandi ikagaragaza neza proteine cyangwa polypeptide. Noneho isomero rya fage risukurwa kugirango rikureho umwanda hamwe nicyiciro kidafunze.

Icyiciro Cyerekana Isomero Uburyo bwo Kugenzura

Intsinzi ya fage yerekana antibody itanga ahanini biterwa na antibody fage yerekana amasomero, kandi uburyo busanzwe bwo gutondekanya fage ni fonctionnement na fonctionnement ya feri. Isuzuma rikomeye rya antigen ikungahaye kuri antibodies zihariye binyuze mu bice 3-4 byo gukuraho kugirango ubone amasomero yerekana antibody; Isuzuma rya Liquid-fase ya antigen ikorwa mugushyiramo intego ya biotin yanditseho intego hamwe na antibody fage yerekana amasomero no gufata ibyiciro biboshye ukoresheje amasaro ya magneti. Isuzuma rikomeye ritwara antigen nyinshi kandi epitopes zimwe na zimwe zirarimbuka. Kugenzura ibice byamazi birangwa no gukora neza no gukungahaza, ariko antibodies zabonetse ntizisobanutse neza. Uburyo bwo gutondekanya fage biterwa nibikenewe mubigeragezo, byose bizagira ingaruka runaka kubitera ingaruka za fage yerekana antibody.

Kwinjiza Antibody Yinshi

Iterambere rya Antibody ryihuse mubuvuzi bwa kijyambere. Uburyo butandukanye bwiterambere rya antibody burahari, ariko kwipimisha hejuru ya antibody ikoreshwa cyane mubumenyi bwubuvuzi. Inzira irashobora kubona ubwinshi bwa antibody repertoire itandukanye. Guteranya hamwe nuburyo gakondo bwo gusuzuma isomero rya antibody birashobora kunoza ireme ryerekanwa ryibitabo byerekana fage. Nyuma yo gusuzuma ibinyabuzima, antibodies zirashobora kurangwa no kwinjiza ELISA nkeya cyangwa kwinjiza antibody nyinshi.

Igishushanyo.1 Imbonerahamwe yerekana uko ibintu byakurikiranye kuva kubaka amasomero ya antibody ya fage kugeza kubyara antibodiyite za monoclonal zifitanye isano cyane na antigene hamwe nubudahangarwa buke.(Inkomoko yerekana: Icyiciro Cyerekana Ikoranabuhanga nkurubuga rukomeye rwo kuvumbura imiti ya Antibody - PMC (nih.gov))

Kwirinda Antibody Phage YerekanaKwirinda

Ibitekerezo bya Buffer

Ihitamo rya buffers rigomba kuzirikana ituze ryintego, guhuza ibidukikije bidukikije, hamwe na bike bidasanzwe bihuza mubikorwa nyirizina. Poroteyine zidafitanye isano, ibikoresho byo mu bwoko bwa ionic, hamwe n’umunyu wa physiologique bifasha kugabanya inyuma. Hariho nibindi bintu bisabwa, nkintego zimwe zishobora gusaba kongeramo ibice bibiri cyangwa izindi cofactors kuri buffer, cofactors za protein zirashobora guhuzwa hamwe nintego, zishobora kongerwaho kubitondekanya, ndetse birashobora no gukoreshwa muburyo bwo gufata intego.

Ibitekerezo

Ibitekerezo byihitirwa mubisanzwe harimo kwibanda kuri molekile yintego, guhuza igihe, gukaraba cyane, nibindi.
Muri byo, icyiciro cya mbere cyo gusuzuma ni ngombwa. Icyiciro cya mbere cyo gusuzuma ni ugufata clon nziza nyinshi zishoboka mubitabo byubatswe mugihe ukuraho clon idasanzwe. Intego zifunitse cyane cyangwa umubare munini wibisubizo bigomba gukoreshwa kugirango harebwe niba umubare munini wibice byafashwe bifatwa kugirango ububiko bwibitabo butandukanye. Mu byiciro byakurikiyeho byo gusuzuma, igitutu cyo guhitamo kigomba kongerwa hamwe no kwiyongera kwikungahaza, gukaraba inshuro nyinshi, no kongera igihe cyo gukaraba, clone zifite isano iri hejuru irashobora kugenzurwa.

Ingamba zo Kugenzura nabi

Ibiranga antigen nibikoresho byabatwara inzira yo gusuzuma ni ugusuzuma ibintu byose biri kuri antigen conjugate, nka tagi, proteyine za fusion, hamwe nubutaka bwunganira. Kugenzura nabi ibitari intego birashobora kugabanya ubukire bwa antibodi uretse antigen.
Utugingo ngengabuzima twose, liposomes, disiki ya nano-fosifolipide, na VLPs birashobora kugenzurwa nabi ukoresheje ingirabuzimafatizo zanduye cyangwa selile zidakingiwe mugihe zerekana imirongo ya selile yahinduwe.
Kurandura imiti ivanze ya antigen igoye irashobora gukorwa kuri molekile itazwi cyangwa igoye, nka selile zose cyangwa proteine zanduye.
Ingamba za antibodies zirwanya imbuga zihariye za antigene, imwe ni ugukuramo antibody ishobora guhangana na ligand wongeyeho ligande nyinshi, naho indi ni iyo guhagarika antibody.

Gushyira mu bikorwa Icyiciro

* Ubuvumbuzi bwa Antibody bwabaye ingirakamaro mubuvuzi bwa kijyambere. Uburyo butandukanye bwo kuvumbura antibody burahari, ariko tekinoroji yo kwerekana fage ikoreshwa cyane mubumenyi bwubuvuzi. Kuva mu 1990, uburyo butandukanye bwa antibody bwakoreshejwe mukubaka amasomero ya fage, harimo VHs, VHHs, scFvs, diabodies, na antibodies za Fab.
Isomero rya Phage peptide ryemerera kumenya byihuse urukurikirane rwa poroteyine kandi byabaye igikoresho gikomeye cyo gukora iperereza ku mikoranire hagati ya epitopi na reseptor antigen.
* Ibice bya Antibody byahujwe na G3P yo mu cyiciro cya M13, kandi mugukwirakwiza ingirabuzimafatizo nyinshi zigizwe na antibody, isomero rinini ryerekana ububiko bwibitabo bwa antibody bushobora kuvamo antibodi nyinshi zitandukanye.
* T7 yerekana ibyiciro bya T7 ifite ibyiza byinshi, birimo ubworoherane, umutekano muke, umutekano, kubika byoroshye, no gutwara, bityo sisitemu ikoreshwa mugukingira indwara no kuvura.
Sisitemu yo kwerekana fage ya T7 irashobora kumenya antigene zitandukanye, nka antigene yo hejuru ya mikorobe itera indwara na antigene za kanseri.

Icyiciro