Urubuga rwo kubaka isomero rya Phage Display Peptide

Alpha Lifetech ifite ikoranabuhanga ry’inyubako za peptide. Dutanga cyane cyane serivisi yo kubaka isomero rya T7 peptide na serivisi yo kubaka isomero rya M13 peptide hakurikijwe ubwoko bwa phage; dutanga serivisi yo kubaka isomero rya peptide 7 na serivisi yo kubaka isomero rya peptide 12 hakurikijwe ingano y’utunyangingo twa peptide, nibindi. Dutanga kandi serivisi yo kubaka isomero rya peptide. Dutanga kandi serivisi zo guhuza no kubaka peptide.

Ikoranabuhanga ryo kwerekana imiterere ya phage nk'uburyo bwo gushyira peptides zikora hanze muri phage ku buso bwa capsid, kubungabunga imiterere n'imikorere ya poroteyine cyangwa peptide yo hanze ntibigira ingaruka ku bushobozi bwa phage recombinant bwo kwanduza ankeli. Buri gace ka virusi ya phage gashobora kugaragaza peptide imwe gusa idasanzwe ku buso bwayo, kandi icyegeranyo cya phage zose zigaragaza peptides zitandukanye kigize isomero rya phage display peptide. Amasomero ya phage display peptides ni ibikoresho bikomeye byo kugaragaza peptides nyinshi zakozwe ku giti cyazo no gusuzuma peptides runaka, kandi byakoreshejwe cyane mu bijyanye no gusesengura epitope ya antigenic, gusuzuma imiti, no guteza imbere urukingo.

Intangiriro ku bwubatsi bw'isomero rya Peptide

Kubaka isomero rya Peptide ni ukugira ngo hongere amasomero ya peptide binyuze mu gushyira urukurikirane rwa peptide ADN mu buryo butandukanye ku rukurikirane rwa poroteyine ya phage shell, no gukoresha phage reassembly kugira ngo peptide target igere ku buso bwa phage. Kubaka isomero rya Peptide hashingiwe ku ikoranabuhanga ryo kwerekana phage ni mu cyiciro cya biosynthesis cya peptide synthesis, kandi hari uburyo bwo gukora peptide y’amazi n’uburyo bwo gukora peptide y’igice gikomeye ku isoko. Kuri ubu, Alpha Lifetech ifite ikoranabuhanga ritandukanye ryo kubaka isomero rya peptide, rishobora gutanga amasomero ya peptide yakozwe mbere n’amasomero ya peptide yatoranijwe hakurikijwe ingano y’urukurikirane rwa peptide, harimo kubaka isomero rya 7-peptide, kubaka isomero rya 12-peptide, kubaka isomero rya 15-peptide, kubaka isomero rya 7-peptide, no kubaka isomero rya 12-peptide, kandi rishobora no guhindurwa kugira ngo rikore isomero rya peptide ku bwoko butandukanye n’uburyo bwo gukoreshwa.

Ikoreshwa ry'inyubako n'isuzuma rya Peptide Library

Amasomero ya peptide yakozwe mu buryo butaziguye hakoreshejwe ikoranabuhanga rya phage display rituma habaho imikorere ya peptide kandi rigatanga isano ifatika hagati ya phenotype (peptide yerekanye) na genotype (DNA yanditswe); aya masomero akwiriye uburyo bwo gusuzuma butandukanya clones ziboshye n'izidaboshye binyuze mu gutunganya affinity. Amasomero ya peptide yerekanye Phage ashobora gukoreshwa mu (i) kugaragaza epitopes z'uturemangingo tw'ubudahangarwa (ii) gusuzuma peptides ziboshye receptor cyangwa protein, peptides ziboshye ku ntego zitari poroteyine, peptides ziboshye ku turemangingo cyangwa ingingo, nibindi na (iii) guteza imbere sisitemu yo gutanga imiti ikoreshwa na peptide.

Inzira y'Isomero rya Peptide yo Kugaragaza Phage

Igishushanyo cya 1 Urubuga rw'Ibyubakwa rya Peptide Library Display rwa Phage

Imikorere y'Isomero rya Peptide rya Phage Display

Inzira	Ibikubiye muri serivisi	Ingengabihe
1. Guhuza urukurikirane rwa nucleotide	Guhuza urukurikirane rw'uturemangingo twa nucleotide mu burebure bwagenwe n'umukiriya	Ibyumweru 2-3
2. Guhindura imiterere y'ibinyabutabire (polymerization) no gusya ibiryo	Guhindura no gusya urukurikirane rwa ADN rwakozwe	Umunsi umwe
3. Kubaka Amasomero yo guhindura amashanyarazi	Bagiteri za ER2738 zarakozwe, hanyuma E. coli ihinduka hakoreshejwe plasmide yubatswe neza	Icyumweru kimwe
4. Ingano y'Isomero rya Peptide n'Uburyo bwo Kumenya Ubudasa	Gupima ingano y'isomero rya peptide hakoreshejwe uburyo bwa koloni n'uburyo butandukanye bwa peptide hakoreshejwe uburyo bwo gukurikirana	Iminsi 2-3
5. Kubaka Isomero rya Peptide rya Phage Display	Mu kwanduza isomero rya peptide yo mu bwoko bwa phage packaging	Icyumweru kimwe