Icyiciro Cyerekana Peptide Isomero

Hashingiwe ku buhanga bwo kwerekana ibyiciro, Alpha Lifetech irashobora guha abakiriya serivisi nziza yo mu cyiciro cya peptide yerekana isomero rya peptide yubaka no kugenzura harimo isomero ry’umurongo wa peptide 7, amasomero 9 ya peptide, amasomero 10 ya peptide, amasomero 15 ya peptide, amasomero 12 ya peptide, amasomero 7 ya peptide, hamwe nandi masomero ya peptide, amasomero atandukanye yerekana ibipimo bitandukanye bya peptide kugira ngo byuzuze ibyiciro bitandukanye bya peptide.

Ibyiciro byerekana amasomero bikoreshwa cyane mubushakashatsi bwibinyabuzima no kubishyira mu bikorwa. Kurugero, mubikorwa bya antibody no guteza imbere antibody, fage yerekana amasomero arashobora gukoreshwa mugusuzuma no kunoza antibody; Mugupima ibiyobyabwenge no kuvumbura intego yibiyobyabwenge, amasomero yerekana fage arashobora gukoreshwa mugushakisha poroteyine cyangwa peptide zifite ibiyobyabwenge. Mu bushakashatsi bw’inkingo, isomero ryerekana ibyiciro rishobora gukoreshwa muguhitamo urukingo rwa antigen no guteza imbere urukingo. Mubyongeyeho, amasomero yerekana fage arashobora kandi gukoreshwa mubice byinshi nkubushakashatsi bwimikoranire ya poroteyine, gusuzuma kanseri no kuvura.

Icyiciro cyo kwerekana ikoranabuhanga

Ikoreshwa rya tekinoroji ya tekinoroji ni tekinoroji ihuza poroteyine igenewe hamwe na poroteyine yo mu bwoko bwa poroteyine, kandi igakora poroteyine igaragara ku gice cya fage mu buryo bwa poroteyine yo guhuza binyuze mu mvugo ya gene. Izi poroteyine zigaragara hejuru yicyiciro zirashobora kugumana imiterere yimiterere nigikorwa cyibinyabuzima, kandi birashobora kugenzurwa, kumenyekana no kumenyekana mugukorana na antibodi zihariye cyangwa izindi molekile. Ibikoresho byo kwerekana fage yerekana tekinoroji harimo antibody, agace ka antibody, igice cya peptide, cDNA, nibindi.

Sisitemu yo kwerekana fage yerekana cyane cyane sisitemu yo kwerekana ibyiciro bya M13 (nka sisitemu ya PⅢ yerekana, PⅧ yerekana sisitemu, nibindi), λ sisitemu yo kwerekana ibyiciro, sisitemu yo kwerekana ibyiciro bya T4, sisitemu yo kwerekana ibyiciro, n'ibindi. Icyiciro cya M13, kubera imiterere ya lysogenic, icyiciro cya M13 ntigishobora gutera selile yakiriye nyuma yo kuyanduza, kikaba cyemerera guhora kwigana no kwerekana ibice byamahanga mumazu yakiriye, bikwiranye no gusuzuma igihe kirekire no kwerekana imvugo ihamye. Kuberako ubuzima bwigihe gito hamwe nubushobozi bwo kwigana cyane, icyiciro cya T7 kirashobora kubyara byihuse umubare munini wibyiciro, bikwiranye no kwinjizamo ibintu byinshi no kwerekana byihuse.

	M13 Icyiciro	T7 Icyiciro
1. Imiterere ya Morphologiya	Icyiciro	Icyiciro cya polyhedrale
2. Ibikoresho bya genetike	Kuzenguruka molekile ya ADN imwe	Umurongo, umurongo-ibiri, ADN irenze urugero
3. Kwigana no kwandura	Ibyiciro bya Lysogenic, byegeranijwe muri periplasme, birashobora gusohoka mu mitsi ya bagiteri nta gutera ingirabuzimafatizo.	Ibyiciro bya virusi, urubyaro rwibyiciro byakusanyirijwe muri cytoplazme ya bagiteri, birekurwa na selile membrane lysis.
4. Erekana Sisitemu	Sisitemu yo kwerekana yubatswe ikoresheje poroteyine ya pIII na PVIII, ikwiranye no kwerekana peptide / proteine zo mu mahanga zingana.	Sisitemu yo kwerekana yubatswe na poroteyine 10B ya capsid, idasaba inzira yo gusohora kandi ikwiriye kwerekana peptide / proteyine zigira ingaruka mbi kubikorwa byo gusohora.
5. Ibyiza byo gusaba	Irakwiriye kwerekanwa no kwerekana igihe kirekire cya peptide ntoya.	Ifite ibyiza mumagambo yihuse hamwe no kwinjiza byinshi bya poroteyine za macromolecular.

Icyiciro Cyerekana Peptide Ubwoko bwibitabo

Alpha Lifetech inararibonye mubyiciro byerekana peptide yubaka isomero no kwerekana kandi ifite ubwoko bwibitabo bwa peptide butandukanye bwo guhitamo. Ukurikije uburebure bwa peptide, irashobora kugabanywamo amasomero 7 ya peptide, amasomero 9 ya peptide, amasomero 10 ya peptide, amasomero 12 ya peptide, n’amasomero 15 ya peptide. Ukurikije ihinduka ryimiterere ya polypeptide, irashobora kugabanywamo isomero rya fage peptide isomero nububiko bwibitabo bwa cyclic phage peptide.

Isomero rya fage peptide isomero

Gene peptide yinjizwa mukarere ka kote proteine yerekana agace ka fage genome, ubusanzwe pIII cyangwa PVIII, kandi ikerekanwa nkumunyururu ugororotse. Umurongo wumurongo uringaniza peptide urashobora kwangirika byoroshye muri vivo, kandi ubushobozi bwabo bwo guhuza no gutuza birashobora kugira ingaruka.

cyclic phage peptide isomero

Impeta ya peptide yahinduwe izakora ihinduka rihamye hifashishijwe ibikorwa bya disulfide cyangwa izindi nkunga zihuza, ibyo ntibizatezimbere cyane ihindagurika ryimiterere no guhindagurika kwa bioavailability, ariko kandi bizuzuza neza ibisabwa guhuza reseptor-ligand (antibody-antigen).

Icyiciro Kugaragaza Isomero rya Peptide

Guhitamo ibice

Ubwoko bwa M13 cyangwa ibyiciro bya T7 mubisanzwe byatoranijwe nkabatwara.

icyiciro Erekana Peptide Kubaka Isomero

Kubaka icyiciro cya fage gishobora kubamo poroteyine yo mumahanga cyangwa polypeptide ya code ikurikirana, mubisanzwe ikubiyemo porotokoro, ibimenyetso byatoranijwe, hamwe nibintu bikwiye. Kwinjiza urutonde rwa code ya poroteyine yo mu mahanga cyangwa peptide mu gutwara fage, mubisanzwe hakoreshejwe uburyo bwo kugabanya imisemburo ya enzyme no guhuza. Inzira ya fage yubatswe ihindurwamo selile yakira, ituma fage yigana kandi ikagaragaza poroteyine zamahanga cyangwa peptide muri selile. Amasomero yicyiciro arimo poroteyine nyinshi zamahanga cyangwa peptide zitegurwa binyuze muguhinduka kwinshi no kongera imbaraga.

icyiciro cyerekana Isomero rya Peptide

Shyiramo molekile zigamije (nka poroteyine, reseptor, cyangwa antibodies) ku cyerekezo gikomeye. Shyiramo fage yubatswe isomero rya peptide isomero hamwe nintego ihamye yo kwemerera peptide kuri fage guhuza na molekile yintego. Muri iki gikorwa, bacteriofage idahambuwe ikurwaho no gukaraba mugihe hagumanye bagiteri zifitanye isano nintego, kandi bagiteri nyinshi zifitanye isano na bagiteri. Indwara ya bacteriofage yagaruwe yongerewe kwanduza Escherichia coli hanyuma ikorerwa icyiciro gikurikira cyo kwisuzumisha. Mubisanzwe bifata ibice 3-5 byo gusuzuma kugirango ubone peptide ikora neza.

Igishushanyo cya peptide hamwe na synthesis

Synthesize peptide ishingiye kurubuga rwo kwerekana.

Igishushanyo.1 Icyiciro cyerekana peptide yubaka isomero nuburyo bwo gusuzuma

Ihuriro

Alpha Lifetech irashobora guha abakiriya serivisi nziza yo mu cyiciro cya peptide yerekana ububiko bwububiko bwibitabo no kwerekana

icyiciro cyerekana antibody isomero-Alpha Lifetech

Icyiciro cyerekana Peptide yububiko bwububiko

Alpha Lifetech ifite tekinoroji yububiko bwububiko bwa peptide. Dutanga cyane cyane serivisi yo kubaka isomero rya T7 peptide na serivisi yo kubaka isomero rya M13 peptide dukurikije ubwoko bwa fage.

SOMA BYINSHI

icyiciro cyerekana kwerekana-Alpha Lifetechphage yerekana kwerekana-Alpha Lifetech

Icyiciro cyerekana Peptide Isomero ryerekana Isomero

Alpha Lifetech ifite uburambe bukomeye mugupima isomero rya peptide, itanga ubufasha bukomeye bwa tekinike yo kuvumbura imiti mito mito, gusuzuma ivuriro treatment kuvura indwara nubushakashatsi bwibinyabuzima.

SOMA BYINSHI