Urubuga rwa Peptide rwa Phage Display

Hashingiwe ku ikoranabuhanga ryo kwerekana phage, Alpha Lifetech ishobora guha abakiriya serivisi nziza zo kubaka no gusuzuma isomero rya phage display peptide harimo amasomero ya linear 7 peptide, amasomero ya linear 9 peptide, amasomero ya linear 10 peptide, amasomero ya linear 15 peptide, amasomero ya linear 12 peptide, amasomero ya cyclic 7 peptide, n'andi masomero ya peptide, ubudasa bw'isomero, igipimo cyo gushyiramo, igipimo cyiza gishobora kugera ku kigero kirenga 90%, kugira ngo gihuze n'ibyo abakiriya batandukanye bakeneye kuri somero za phage display peptide.

Amasomero yo kwerekana ibimenyetso bya phage akoreshwa cyane mu bushakashatsi no mu mikoreshereze y’ubuvuzi. Urugero, mu gukora inyigo y’uturemangingo no guteza imbere uturemangingo, amasomero yo kwerekana ibimenyetso bya phage arashobora gukoreshwa mu gusuzuma no kunoza ubwoko bw’uturemangingo; Mu gusuzuma imiti no kuvumbura intego z’imiti, amasomero yo kwerekana ibimenyetso bya phage arashobora gukoreshwa mu gushakisha poroteyine cyangwa peptides zifite ubushobozi bwo gufata imiti. Mu bushakashatsi ku rukingo, isomero ryo kwerekana ibimenyetso bya phage rishobora gukoreshwa mu guhitamo antigen y’urukingo no guteza imbere urukingo. Byongeye kandi, amasomero yo kwerekana ibimenyetso bya phage arashobora kandi gukoreshwa mu nzego nyinshi nko mu bushakashatsi ku mikoranire ya poroteyine, gusuzuma kanseri no kuvura.

Ikoranabuhanga ryo kwerekana phage

Ikoranabuhanga ryo kwerekana phage ni ikoranabuhanga rihuza gene ya poroteyine y’ingenzi na gene ya poroteyine y’ubuso bwa phage, kandi rigatuma poroteyine y’ingenzi iboneka ku buso bwa phage mu buryo bwa poroteyine ivanze binyuze mu mikorere ya gene. Izi poroteyine zigaragara ku buso bwa phage zishobora kugumana imiterere y’ahantu n’imikorere ya biyoloji, kandi zishobora gupimwa, kumenyekana no kumenyekana hakoreshejwe uburyo bwo gukorana n’uturemangingo twihariye tw’umubiri cyangwa izindi molecule. Ibintu by’ikoranabuhanga ryo kwerekana phage birimo antibody, antibody fragment, peptide segment, cDNA, nibindi.

Sisitemu y'ikoranabuhanga ryo kwerekana phage igizwe ahanini na sisitemu yo kwerekana phage ya M13 (nk'isitemu yo kwerekana PⅢ, sisitemu yo kwerekana PⅧ, nibindi), sisitemu yo kwerekana phage ya λ, sisitemu yo kwerekana phage ya T4, sisitemu yo kwerekana phage ya T7, nibindi. Buri sisitemu ifite imiterere yayo kandi ikwiriye ubushakashatsi butandukanye. Phage ya M13, bitewe n'imiterere yayo ya lysogenic, phage ya M13 ntabwo ikora ingirabuzimafatizo nyuma yo kuyitera, ibi bigatuma ikomeza kwisubiramo no kugaragaza ibice by'amahanga mu ngirabuzimafatizo, bikwiye gupimwa igihe kirekire no kugaragaza neza. Kubera ko imara igihe gito kandi ikora neza cyane, phage ya T7 ishobora gukora vuba umubare munini w'ingirabuzimafatizo, zikwiriye gupimwa neza no kugaragaza vuba.

	M13 Phage	T7 Phage
1. Imiterere y'imiterere	Fage ifite ubudodo	Ifuru ya polyhedral
2. Ibikoresho by'ingirabuzimafatizo	Molekyuli ya ADN ifite umugozi umwe izengurutse	ADN ifite umurongo, ifite imigozi ibiri, idahagije ku buryo burambye
3. Kwiyongera no kwandura indwara	Uduce twa Lysogenic phages, dukusanyirijwe muri periplasm, dushobora gukurwa mu ruhu rw'uturemangingo twa bagiteri tudakoresheje lysing y'uturemangingo twakira.	Udukoko twa virusi, urubyaro rwa utwo dukoko twahuriye muri cytoplasm ya bagiteri, durekurwa na lysis ya selile.
4. Sisitemu yo kwerekana	Sisitemu yo kwerekana yakozwe hakoreshejwe poroteyine za capsid za pIII na PVIII, zikwiriye kwerekana peptides/poroteyine z'amahanga z'ingano zitandukanye.	Sisitemu yo kwerekana ikozwe muri poroteyine ya capsid ya 10B, idasaba uburyo bwo gusohora kandi ikwiriye kwerekana peptides/poroteyine zigira ingaruka ku buryo bwo gusohora.
5. Akamaro k'ikoreshwa	Ikwiriye kwerekana no gusuzuma peptides nto za molekile mu gihe kirekire.	Ifite akamaro ko gukora vuba no gusuzuma neza poroteyine za macromolecular.

Ubwoko bw'Isomero rya Peptide rya Phage Display

Alpha Lifetech ifite uburambe mu kubaka no gusuzuma isomero rya phage display peptide, kandi ifite ubwoko butandukanye bw'isomero rya peptide. Dukurikije uburebure bwa peptide, ishobora kugabanywamo amasomero 7 ya peptide, amasomero 9 ya peptide, amasomero 10 ya peptide, amasomero 12 ya peptide, n'amasomero 15 ya peptide. Dukurikije imiterere y'ahantu ya polypeptide, ishobora kugabanywamo isomero rya phage peptide rigororotse n'isomero rya phage peptide rizenguruka.

Isomero rya peptide ya phage y'umurongo

Ingirabuzimafatizo za peptide zishyirwa mu gace gafite poroteyine ya phage genome, akenshi pIII cyangwa PVIII, kandi zigaragazwa nk'urunigi rugororotse. Ingirabuzimafatizo za linear linear chain zishobora kwangirika byoroshye mu mubiri, kandi ubushobozi bwazo bwo gufatana no kugumana kwazo bishobora kugira ingaruka.

isomero rya peptide ya cyclic phage

Peptide y'impeta yahinduwe izarema imiterere idahinduka bitewe n'ibikorwa bya disulfide bonds cyangwa izindi mpande zombi, ibyo ntibizanoza cyane gusa imikorere ya metabolisme no kuba bioavailability, ahubwo bizanahuza neza n'ibisabwa mu mibanire ya receptor-ligand (antibody-antigen).

Inzira y'Isomero rya Peptide yo Kugaragaza Phage

Guhitamo phage

Ubusanzwe, uduce twa M13 cyangwa uduce twa T7 dufatwa nk'abatwara.

Kubaka Isomero rya Peptide Display ya phage

Kora vektori ya phage ishobora kuba irimo urukurikirane rw’inyandiko za poroteyine cyangwa polypeptide zo mu mahanga, ubusanzwe zirimo promoteri, ibimenyetso byo guhitamo, n’ibintu bikwiye byo kugenzura. Gushyira urukurikirane rw’inyandiko za poroteyine cyangwa peptide zo mu mahanga mu gikoresho cya phage, ubusanzwe hakoreshejwe uburyo bwo gukata no gushyiramo enzyme. Vektori ya phage yubatswe ihinduka ingirabuzimafatizo, bigatuma phage yongera kandi ikagaragaza poroteyine cyangwa peptide zo mu mahanga mu gikoresho. Amasomero ya phage arimo poroteyine cyangwa peptide zo mu mahanga zitandukanye ategurwa binyuze mu ihinduka rikomeye no kongera imbaraga.

Ishusho ya phage Isuzuma rya Peptide Library

Shyira molekile zigamije (nk'amaporoteyine, receptor, cyangwa antibodies) ku gikoresho gifata ibintu bikomeye. Shyira isomero rya peptide yubatswe na phage display hamwe n'intego ihamye kugira ngo peptide ziri kuri phage zifatanye na molekile. Muri iki gikorwa, bacteriophages zidafite aho zibohewe zikurwaho no gukaraba mu gihe bacteriophages zifatanye n'intego, kandi bacteriophages zifatanye cyane zirakurwaho. Bacteriophages zabonetse zongerwamo imbaraga mu kwanduza Escherichia coli hanyuma zigakorerwa isuzuma rikurikira. Akenshi bifata isuzuma ry'amasaha 3-5 kugira ngo haboneke peptide zifite ubushobozi bwo gufatana neza.

Igishushanyo n'ikorwa rya peptide

Gukora peptides hashingiwe ku mbuga zo gupima.

Igishushanyo cya 1. Uburyo bwo kubaka no gusuzuma isomero rya peptide yerekana imiterere ya Phage

Urubuga rujyanye

Alpha Lifetech ishobora guha abakiriya serivisi nziza zo kubaka no gusuzuma isomero rya phage display peptide.

isomero ry'umubiri urwanya indwara ya phage display - Alpha Lifetech

Urubuga rwo kubaka isomero rya Phage Display Peptide

Alpha Lifetech ifite ikoranabuhanga ry’inyubako za peptide. Dutanga cyane cyane serivisi yo kubaka isomero rya T7 peptide na serivisi yo kubaka isomero rya M13 peptide hakurikijwe ubwoko bwa phage.

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gusuzuma ikimenyetso cya phage-Gusuzuma ikimenyetso cya Alpha Lifetechphage-Alpha Lifetech

Urubuga rwo gusuzuma isomero rya Phage Display Peptide

Alpha Lifetech ifite ubunararibonye bwinshi mu gusuzuma isomero rya peptide phage, itanga inkunga ikomeye mu buhanga mu kuvumbura imiti mito, gusuzuma indwara, kuvura indwara no gukora ubushakashatsi ku binyabuzima.

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