Sisitemu yo kwerekana icyiciro
Alpha Lifetech yubatse sisitemu ihamye ya Phage yerekana uburyo butwara igihe cyubushakashatsi bwa siyanse cyangwa ubushakashatsi bwumushinga kandi byoroshya umusaruro ukurikira
TWANDIKIRE01
Sisitemu yo kwerekana icyiciro
Alpha Lifetech amaze imyaka myinshi akora cyane mubuhanga bwo kwerekana Phage. Alpha Lifetech yubatse neza uburyo bwa tekinoroji ya Phage yerekana tekinoroji itwara umwanya kubushakashatsi bwa siyanse cyangwa ubushakashatsi bwumushinga kandi byoroshya umusaruro. Dufite sisitemu nyinshi zo kwerekana ibyiciro kubakiriya bahitamo, harimo icyiciro cya M13, T7 fage, T4 fage, na λ fage yerekana sisitemu. Ukurikije ibyo ukeneye mubigeragezo, urashobora guhitamo sisitemu yo kwerekana ibyiciro. Alpha Lifetech irashobora kandi gutanga serivisi kubakiriya nka vhh antibody, umusaruro wa scfv antibody, hamwe na Fab antibody.
Intangiriro kuri Phage yerekana
Ikoreshwa rya tekinoroji yerekana ni tekinike ikoresha ibice (virusi yanduza bagiteri) gushakisha molekile ikora ya poroteyine cyangwa peptide yihariye. Ubuhanga bwububiko bwibitabo bwa Phage antibody burimo kubaka ububiko bwibitabo bwa Fab antibody, kubaka isomero rya antibody ya scFv, kubaka isomero rya vhh antibody, nibindi. Ukurikije ubwoko bwa bacteriofage, birashobora kugabanywa muri bacteriophage m13, bacteriophage T4, bacteriophage, λ bacteriophage, na bacteriophage. Ukurikije ubwoko bwibitabo, burashobora kugabanywa mububiko bwibitabo bwa peptide, isomero rya cDNA, isomero rya antibody, nububiko bwa protein. Icyiciro cyo kwerekana tekinoroji iroroshye gukora kandi ihendutse gukoresha irimo Fab yerekana fage, kwerekana fage yerekana, vhh yerekana. Nyamara, ubu buhanga bugabanya itandukaniro ryimiterere ya molekuline mu isomero ridashobora kwerekana igihe kirekire.
Ikoreshwa rya tekinoroji yerekana ubushobozi bushobora gukora isano iri hagati ya molekile yerekanwe hamwe na ADN ikurikirana ikubiyemo molekile yerekanwe, kandi aside amine ikurikirana ya conjugate yihariye irashobora kugenwa ako kanya na ADN ikurikirana igice cyifuzwa muri genome ya fage.
Kugeza ubu, tekinoroji yo kwerekana ikoreshwa cyane. Muri byo, hari byinshi byagezweho mu bushakashatsi no guteza imbere inkingo nshya (inkingo zidahenze kandi zikora neza), guteza imbere imiti ya antibody (kwerekana enzyme inhibitori), kwanduza ibimenyetso bya selile (kwerekana epitopi yigana), no gukora ubushakashatsi kuri epitopi ya antigen (gutegura antibodiyite za monoclonal).
Ihame ryo kwerekana fage
Icyiciro cyerekana ni tekinoroji ya molekuline ishingiye ku guhindura imiterere ya ADN ya fage, ihuza igice cyerekanwe na proteine ya fage ikote bityo ikagaragaza hejuru ya fage. Urutonde rwa ADN yo mu mahanga rwinjijwe ahantu runaka mu buryo bwa nucleotide ya genome ya fage igizwe na poroteyine ya fage (Ishusho.1).
Iyo indwara ya fage ibaye, gene ya fage itangira kwigaragaza muri bagiteri yakiriye, kandi igice cyinjijwemo cyerekanwe hejuru yicyiciro nkikomatanya rya gene ifitanye isano na kote ya proteine hamwe nurutonde rwa clon.
Igishushanyo.1 Bacteriophage M13 ubwubatsi bwa virusi hamwe nihame rya tekinoroji yo kwerekana.(Inkomoko yerekana: Icyiciro cyo kwerekana hamwe nubundi buryo bwa peptide yerekana tekinoroji | Microbiology FEMS Isubiramo | Oxford Ushinzwe amasomo (oup.com))
Icyiciro cyerekana Sisitemu
Ibyiciro bya filime nibisanzwe bikoreshwa muri sisitemu yo kwerekana ibyiciro, kandi sisitemu yo kwerekana ibyiciro irashobora kubakwa hakoreshejwe ibindi byiciro. Sisitemu nkiyi irashobora kwerekana peptide nini cyangwa ecran ya cDNA isomero neza kuruta sisitemu ishingiye kuri fage.
Igishushanyo.2 Ubwoko bwa bacteriofage ikoreshwa mugice cyo kwerekana.(Inkomoko yerekana: Icyiciro cyo kwerekana hamwe nubundi buryo bwa peptide yerekana tekinoroji | Microbiology FEMS Isubiramo | Oxford Ushinzwe amasomo (oup.com))
Ibyiza bya sisitemu yo kwerekana
Sisitemu yo kwerekana ibyiciro mubisanzwe ikoresha ibyiciro, harimo M13, fd, na f1. Ugereranije nicyiciro cya M13, Teri ya bacteriophage T7 igizwe na ADN igizwe n'imirongo ibiri, igaragaza ituze ryinshi kandi ikaba idakunze guhinduka mugihe cyo kwigana. T7 fage, nkuwitwaye, ikura vuba kandi irashobora gukora plaque muri 3h, ikabika umwanya munini wo gukoroniza no kwerekana.
Kubaka isomero rya T7 ryakoreshejwe muburyo bwo kwerekana imiterere ya gene no hejuru yubuso no kubaka amasomero yerekana peptide, cDNA, ADN genomic, na antibodies. Ibi bituma icyiciro cya T7 kidakoreshwa gusa nka mikorobe yica mikorobe gusa, ahubwo no mugupima ibiyobyabwenge mugupima ingingo (nkubwonko, umwijima, nibihaha), gusuzuma intego yo kumenya antibody, ubushakashatsi bwibikorwa bya physiologique, nibindi birashobora no gukoreshwa mugupima no kuvura indwara zitandukanye.
Ubundi buryo bwa bagiteri
Icyiciro cyanduye
Bitandukanye na fage gakondo yerekana, icyiciro cyanduye cyanduye (SIP) gihuza umusaruro wa protein-ligand umusaruro wanduye no kwandura fage no kwaguka bitabaye ngombwa ko habaho intambwe zo gukuraho.
Igishushanyo.3 Icyiciro cyanduye (SIP).(Inkomoko yerekana: Icyiciro cyo kwerekana hamwe nubundi buryo bwa peptide yerekana tekinoroji | Microbiology FEMS Isubiramo | Oxford Ushinzwe amasomo (oup.com))
Ibice nyaburanga
Ibice nyaburanga biterwa na kopi ibihumbi n’ibihumbi bya peptide y’amahanga itondekanye hafi ya tubular capside muburyo bwo gusubiramo cyane, kandi kwerekana polyvalent kumiterere yimiterere itera guhitamo abantu benshi, kubwibyo ntibishoboka gutandukanya isano iri hejuru na nto ya peptide ligand.
Igishushanyo.4 Igitekerezo cyibice byimiterere. (Inkomoko yerekana: Icyiciro cyo kwerekana hamwe nubundi buryo bwa peptide yerekana tekinoroji | Microbiology FEMS Isubiramo | Oxford Ushinzwe amasomo (oup.com))
Sisitemu nyinshi zerekana sisitemu
Icyiciro cya M13, T7 icyiciro, T4 icyiciro, λ icyiciro cyo kwerekana sisitemu kubakiriya bahitamo.
Ubushobozi bwo kwinjiza ibintu byinshi
Turashobora gukoresha fage yerekana sisitemu kugirango twubake byihuse amasomero manini y'ibitabo, kandi tugere kubushakashatsi bwimbitse.
Serivisi yihariye yerekana serivisi
Abakiriya barashobora guhitamo serivisi zuzuye bakurikije ibyifuzo byumushinga, nko kwerekana antibodiyite nyinshi, nibindi.
Urutonde rwagutse
Bikoreshwa mubice nka antibody yubuhanga, peptide na protein yubuhanga, kuvumbura ibiyobyabwenge niterambere, ubushakashatsi bwimikoranire ya poroteyine, nibindi.
Niba ufite ikibazo, nyamuneka twandikire igihe icyo aricyo cyose.
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2018-07-16 
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