Sisitemu yo Kugaragaza Phage

Alpha Lifetech imaze imyaka myinshi ikora cyane mu ikoranabuhanga ryo kwerekana Phage. Alpha Lifetech yubatse urubuga rwiza rw’ikoranabuhanga ryo kwerekana Phage rudahindagurika rutuma abakiriya batakaza umwanya wo gukora ubushakashatsi bwa siyansi cyangwa ubushakashatsi ku mishinga, kandi rukorohereza umusaruro ukurikiraho. Dufite sisitemu nyinshi zo kwerekana phage ziboneka ku bakiriya, harimo M13 phage, T7 phage, T4 phage, na λ phage display. Bitewe n’ibyo ukeneye mu igerageza, ushobora guhitamo sisitemu ikwiye yo kwerekana phage. Alpha Lifetech ishobora kandi gutanga serivisi ku bakiriya nko gukora antibody ya vhh, gukora antibody ya scfv, no gukora antibody ya Fab.

Intangiriro ku imurikagurisha rya Phage

Ikoranabuhanga ryo kwerekana udukoko duto ni uburyo bukoresha udukoko duto (virusi itera bagiteri) kugira ngo bashakishe molecules zihuza poroteyine cyangwa peptides runaka. Uburyo bwo kubaka isomero rya antibody ya Phage burimo kubaka isomero rya antibody ya Fab antibody, kubaka isomero rya antibody ya scFv, kubaka isomero rya antibody ya vhh, nibindi. Dukurikije ubwoko bwa bacteriophages, zishobora kugabanywamo bacteriophage m13, bacteriophage ya T7, bacteriophage ya T4, bacteriophage ya λ, n'izindi bacteriophage. Dukurikije ubwoko bw'isomero, ishobora kugabanywamo isomero rya peptide ridasanzwe, isomero rya cDNA, isomero rya antibody, n'isomero rya poroteyine. Ikoranabuhanga ryo kwerekana udukoko duto ni ryoroshye gukoresha kandi rihendutse kurikoresha ririmo isomero rya Fab phage, isomero rya scfv phage, isomero rya vhh phage. Ariko, ubu buryo bugabanya ubudasa bw'uturemangingo tw'uturemangingo mu isomero tudashobora kugaragaza urukurikirane rurerure cyane.

Ikoranabuhanga ryo kwerekana phage rishobora gukora isano ifatika hagati ya molekile igaragara n'uruhererekane rwa ADN rugaragara, kandi uruhererekane rwa aside amine y'igice runaka gishobora kumenyekana ako kanya hakoreshejwe uburyo bwa ADN bw'igice cyifuzwa muri genome ya phage.

Muri iki gihe, ikoranabuhanga ryo kwerekana phage rikoreshwa cyane. Muri byo, hari byinshi byagezweho mu bushakashatsi no guteza imbere inkingo nshya (inkingo zihendutse kandi zikora neza), iterambere ry'imiti irwanya indwara (ikingira enzymes zo gusuzuma), ikwirakwizwa ry'ibimenyetso by'uturemangingo (gusuzuma epitopes zakozwe), n'ubushakashatsi ku epitopes za antigen (gutegura antibody za monoclonal).

Ihame ryo kwerekana phage

Ishusho ya phage ni ikoranabuhanga rya molekile rishingiye ku guhindura ADN ya phage, rihuza igice cy’ingenzi na poroteyine ya phage coat bityo riyigaragaza ku buso bwa phage. Ishusho ya ADN y’amahanga yinjizwa ahantu runaka mu gice cya nucleotide cya genome ya phage ishyiraho poroteyine ya phage coat (Ishusho ya 1).

Iyo habayeho ubwandu bwa phage, phage gene itangira kugaragara mu mubiri wa bagiteri, kandi igice cy’ingenzi cy’iyi gene kigaragara ku buso bwa phage nk’uruvange rwa gene ifitanye isano ikubiyemo poroteyine y’agahu n’urukurikirane rwa cloned.

Igishushanyo cya 1 Imiterere ya Bacteriophage M13 virion n'amahame y'ikoranabuhanga ryo kwerekana phage.(Inkomoko y'aho uherereye: Icyerekanwa cya Phage n'ubundi buryo bwo kwerekana peptide | Isuzuma rya FEMS Microbiology | Oxford Academic (oup.com))

Sisitemu zo kwerekana phage

Filamentous phages niyo sisitemu yo kwerekana phage ikoreshwa cyane, kandi sisitemu yo kwerekana phage ishobora gukorwa hakoreshejwe izindi phage. Izi sisitemu zishobora kwerekana peptide nini cyangwa isomero rya cDNA rya screen neza kurusha sisitemu zishingiye kuri filamentous phage.

Igishushanyo cya 2 Ubwoko bwa bagiteriofagi zikoreshwa mu kwerekana phage.(Inkomoko y'aho uherereye: Icyerekanwa cya Phage n'ubundi buryo bwo kwerekana peptide | Isuzuma rya FEMS Microbiology | Oxford Academic (oup.com))

Akamaro ka sisitemu yo kwerekana Phage

Ubusanzwe sisitemu zo kwerekana uduce duto (Phage display systems) zikoresha uduce duto (filamentous phages), harimo M13, fd, na f1. Ugereranyije na phage za M13, uduce duto twa bacteriophage twa T7 tugizwe na ADN ifite imigozi ibiri, igaragaza ubusugire bukomeye kandi ntishobora guhinduka cyane mu gihe cyo kwibasira. T7 phage, nk'umutwaro, ikura vuba kandi ishobora gukora plaque mu masaha 3, ibi bikaba bizigama igihe kinini cyo gushushanya no gusuzuma.

Imiterere y'isomero rya T7 phage yakoreshejwe mu kugaragaza imiterere y'uturemangingo tw'umubiri ku buso no ku buso, ndetse no mu kubaka amasomero yerekana peptides, cDNA, ADN ya genome, na antibody. Ibi bituma T7 phage idakoreshwa gusa nk'umuti urwanya udukoko, ahubwo inakoreshwa mu gusuzuma imiti mu gusuzuma ingingo z'umubiri (nk'ubwonko, umwijima, n'ibihaha), gusuzuma ingingo z'umubiri, ubushakashatsi ku mikorere y'umubiri, n'ibindi. Ishobora kandi gukoreshwa mu gusuzuma no kuvura indwara zitandukanye.

Ubundi bwoko bwa bagiteriofagi

Fage ifata ubwandu mu buryo bwihariye

Bitandukanye n’uburyo gakondo bwo kugaragaza phage, phage itoranya indwara (SIP) ihuza imikorere ya poroteyine na ligand mu buryo butaziguye n’ubwandu bwa phage no kwaguka kwayo hatabayeho ingamba zo kubikemura.

Igishushanyo cya 3. Indwara yandurira mu buryo bwihariye (SIP).(Inkomoko y'aho uherereye: Icyerekanwa cya Phage n'ubundi buryo bwo kwerekana peptide | Isuzuma rya FEMS Microbiology | Oxford Academic (oup.com))

Imiterere y'ubutaka

Ingirabuzimafatizo zo mu butaka ziterwa n'ibihumbi by'amakopi ya peptides zo mu mahanga zikikije capsids zo mu tubulari mu buryo buhamye cyane bwo gusubiramo, kandi igaragara rya polyvalent kuri landscape phages rituma habaho guhitamo ubwiza buhanitse, bityo ntibishoboka gutandukanya ubwiza buhanitse n'ubuke bwa peptide ligands.

Igishushanyo cya 4 Igitekerezo cy'imiterere y'ubutaka. (Inkomoko y'aho uherereye: Icyerekanwa cya Phage n'ubundi buryo bwo kwerekana peptide | Isuzuma rya FEMS Microbiology | Oxford Academic (oup.com))

Ibyiza bya sisitemu zo kwerekana phage

Sisitemu nyinshi zo kwerekana phage

Sisitemu yo kwerekana M13 phage, T7 phage, T4 phage, λ phage ku bakiriya bashobora guhitamo.

Ubushobozi bwo gusuzuma umusaruro mwinshi

Dushobora gukoresha sisitemu zo kwerekana phage kugira ngo twubake vuba amasomero manini ya phage, kandi tugere ku igerageza ryo gusuzuma rigezweho.

Serivisi yo kwerekana phage yihariye

Abakiriya bashobora guhindura serivisi zuzuye hakurikijwe ibisabwa n'umushinga, nko gusuzuma antibodies zirwanya indwara nyinshi, n'ibindi.

Urutonde rw'ibikoresho bikoreshwa mu buryo bwagutse

Ikoreshwa mu nzego nko mu bijyanye no gupima abarwayi banduye, peptide na poroteyine, kuvumbura no guteza imbere imiti, ubushakashatsi ku mikoranire ya poroteyine, n'ibindi.

Sisitemu zo Kugaragaza Phage

Dufite sisitemu nyinshi zo kwerekana phage zihari ku bakiriya, harimo M13 phage, T7 phage, T4 phage, na λ phage display system.

Ikimenyetso cya M13 phage - Alpha Lifetech

Urubuga rwa T7 Phage Library

Alpha Lifetech ifite urubuga rwagutse rwo kwerekana T7 phage kugira ngo ikoreshwe mu bubiko bw'amasomero y'abashinzwe kurwanya indwara.

Urubuga rwa M13 Phage Library

Alpha Lifetech afite uburambe bw'imyaka icumi mu kubaka isomero rya M13 phage no gusuzuma imbonerahamwe ya M13 phage.

Urubuga rwo kwerekana Lambda Phage

Alpha Lifetech ifite urubuga rwo kwerekana Lambda phage kugira ngo ikomeze kubaka no gukora amasomero y’abashinzwe kurwanya indwara.

Urubuga rwo kwerekana T4 Phage

Twubaka kandi urubuga rwo kwerekana T4 phage kugira ngo abakiriya bahitemo.

Niba ufite ikibazo icyo ari cyo cyose, twandikire igihe icyo ari cyo cyose.

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