Kuki twahitamo Poroteyine yacu ya Recombinant?

Ubwiza Buhanitse:Poroteyine zacu zivanze zikorwa hakoreshejwe ikoranabuhanga rigezweho kugira ngo zigire isuku ihebuje no gukuraho imyanda ishobora kwangiza imikorere yazo.

Umusaruro mwinshi:Binyuze mu buryo bunoze bwo gukora, ibicuruzwa byacu bya poroteyine bivanze bifite akamaro kanini kandi bishobora kugira akamaro kanini mu buryo butandukanye.

Ubwiza buhamye:Buri gice cya poroteyine zacu zivanze gikurikiza ingamba zikomeye zo kugenzura ubuziranenge kugira ngo gikomeze kugira ubuziranenge n'imikorere ihamye, bigaha abashakashatsi n'abahanga mu bya siyansi ibisubizo byizewe buri gihe.

Ihendutse ku giciro:Dusobanukiwe akamaro ko gutanga agaciro tudatesheje agaciro ubuziranenge. Mu guhitamo Alpha Lifetech, ubona poroteyine nziza zo mu bwoko bwa recombinant ku giciro cyiza, bikongera ingengo y'imari yawe y'ubushakashatsi utitaye ku buziranenge bw'igerageza.

Urutonde rwa Poroteyine Zivanze

Ibikoresho bya poroteyine bivanze

Alpha Lifetech ifite ibicuruzwa birenga 2000 bya poroteyine zivanze kandi ikorana neza n'abakiriya ibihumbi b'ibigo mpuzamahanga buri mwaka kugira ngo ibahe ibisubizo. Fungura ubushobozi bw'ikoranabuhanga ryo gutanga poroteyine zivanze ukoresheje ibicuruzwa na serivisi zacu byiza bya poroteyine zivanze. Twandikire uyu munsi kugira ngo wihutishe imishinga yawe ukoresheje serivisi zo gutanga poroteyine zivanze n'ibicuruzwa bya poroteyine zivanze.

Poroteyine Ihuza Ibikoresho Bitandukanye-Alpha Lifetechnqf

Poroteyine ivanze ivura kanseri

Poroteyine ya Recombinant2-Alpha Lifetech4o5

Poroteyine ya Recombinant2-Alpha Lifetechk4z

Poroteyine ya Recombinant3-Alpha Lifetechcfl

Poroteyine yo mu bwoko bwa Recombinant3-Alpha Lifetechbyj

Poroteyine ya Recombinant1-Alpha Lifetechhrb

Poroteyine yo mu bwoko bwa Recombinant1-Alpha Lifetech649

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Ibindi bicuruzwa

Serivisi yo gukora poroteyine zivanze ku buryo bwihariye

Mu myaka 30 ishize, habayeho iterambere rikomeye mu ikoranabuhanga ryo kugaragaza poroteyine zihuza.Alpha Lifetech Inc.itanga uburyo bune bw'ingenzi bwo kugaragaza poroteyine kugira ngo ikore poroteyine zihuza: Bagiteri, umusemburo, uturemangingo tw'udukoko, n'uturemangingo tw'inyamabere.Alpha Lifetech Inc.Itanga urutonde rw'poroteyine zivanze ziturutse ku bintu bitandukanye, n'ubwoko butandukanye, ibyo bicuruzwa bishobora gukoreshwa mu bushakashatsi no mu gusuzuma indwara. Poroteyine zivanze zirimo enzymes, ibintu bikura, cytokines, poroteyine za Fc receptor, poroteyine zo kugenzura ubudahangarwa bw'umubiri, na poroteyine zo mu ruhu. Izi poroteyine zivanze zishobora gukoreshwa mu bikorwa bitandukanye, harimo culture y'uturemangingo, bioassays, no gusuzuma indwara muri vitro.

Kugereranya Sisitemu yo Gusobanura Poroteyine Ivanze

Sisitemu zo Gusobanura Ibyumviro

Bagiteri

Sisitemu

Umusemburo

Sisitemu

Virusi ya Baculovirus

-udukoko

Sisitemu

Inyamabere

Sisitemu

Akamaro

Igiciro gito

Kuzigama igihe

Ihendutse ku giciro

kwagura inganda

Iterambere ryihuse

impinduka zakozwe nyuma yo guhindura

Hagaragara neza imiterere y'uturemangingo

Imiterere yoroshye yo guhinga

Umutekano mwinshi

Igaragaza imikorere myiza cyane

guhindura nyuma y'ubuhinduzi

Uburozi buke

Umutekano ukomeye

Ibiranga gukura guhamye

Byoroshye guhingwa no guhindurwa

Igaragaza imikorere myiza cyane

Imbogamizi

Kwangirika kwa poroteyine

Ibibazo by'uburozi

Ubuhumane bw'uburozi bw'imbere mu mubiri

Kudashonga kwa poroteyine

Umusaruro muke

Poroteyine nkeya Ikwiriye gato mu kugaragaza poroteyine nini kandi zidafite ubwiyongere bwinshi bw'amazi.

Ubushobozi buke bwo gukora poroteyine zifite uburemere bwinshi bwa molekile; Nta bushobozi buke mu gukora poroteyine zo mu ruhererekane

Ikiguzi kiri hejuru

Igihe kirekire cyo gukora

Ibisabwa mu muco ugoye

Porogaramu

Inyigo z'imiterere

Gukora poroteyine nto kandi zishobora gushonga

Gukora enzymes mu nganda

Inyigo z'imikorere

poroteyine zikozwe mu buryo bugoye;

Guteza imbere inkingo

Ivumburwa ry'ibiyobyabwenge

Inyigo ku mikoranire ya poroteyine na poroteyine

Ibibazo Bikunze Kubazwa Ku Bicuruzwa Bivanze bya Poroteyine

Imishinga yacuTuragukorera byose!

Ishusho ya 1: Ibisubizo bya SDS-PAGE byo gutunganya poroteyine

M: Ikimenyetso; Umurongo wa 1: Rukawa; Umurongo wa 2-8: Icyitegererezo cya poroteyine isukuye

Umusaruro wa Poroteyine Ishonga mu Mutwe wa E. coli

Intego Kwiga imiterere ya poroteyine zivanze cyane zishobora gushonga.
Ikibazo Mbere na mbere byagaragaye muri E. coli, hakozwe ibinyabutabire bidashonga kandi bisaba gupfunyika.
Ibisubizo Gahunda nziza yongereye umusaruro wa poroteyine ishongeshwa kuva kuri

Ishusho ya 2: Ibisubizo bya SDS-PAGE by’igaragara n’isukurwa ry’uturemangingo tw’inyamabere (ubuziranenge >90%)

Impinduka mu miterere y'uturemangingo tw'inyamabere mu guhindura nyuma yo guhindura

Intego Gukora isuzuma ry'imikorere ya poroteyine ya glycosylated.
Ikibazo Sisitemu ya mbere ya E. coli na sisitemu yo kugaragaza umusemburo yananiwe gutanga impinduka nziza nyuma yo guhindura (PTM).
Ibisubizo Gutunganya neza byatumye habaho uburinganire bw'isukari ikenewe >90%.

Ishusho ya 3: Ibisubizo bya WB byo gutunganya poroteyine

Igaragaza rya poroteyine idafite utunyangingo ku poroteyine z'uburozi

Intego Kugira ngo habeho poroteyine ifitanye isano n'ururabo ihumanya uturemangingo tuzima.
Ikibazo Uburyo gakondo bwo kuvuga ibintu ntabwo bukwiye.
Ibisubizo Uburyo bwo kutagira uturemangingo bwatanze poroteyine zikora neza ku gipimo cya 2-3mg/mL, birenga uburozi bw’uturemangingo.

1. Ni gute nasaba ibiciro ku bicuruzwa byinshi?

Hitamo ibicuruzwa cyangwa ibicuruzwa wifuza kugura ku bwinshi. Ibi bishobora kuba birimo poroteyine zivanze, antibodies, cyangwa ibindi bintu birwanya indwara. Twandikire kuri info@alpha-lifetech.com
2. Nabona nte COA ya poroteyine ya recombinant nahawe?

Twandikire ukoresheje imeri info@alpha-lifetech.com kugira ngo ubone amakuru arambuye kuri iki gicuruzwa.
3. Poroteyine ya recombinant ni iki?

Poroteyine ya recombinant ni poroteyine ikorwa hakoreshejwe ikoranabuhanga rya ADN ya recombinant, akenshi binyuze mu kugaragaza gene ishishikajwe n'ibinyabuzima bitandukanye, nka bagiteri, umusemburo, cyangwa uturemangingo tw'inyamabere. Iri koranabuhanga ryemerera gukora poroteyine zidahagije cyangwa zigoye kubona mu masoko yazo. Alpha Lifetech ishobora gutanga poroteyine ya recombinant.
4. Ni ibihe bintu byo kohereza poroteyine zivanze?

Poroteyine zivanze akenshi zoherezwa ku rubura rwumye (−78.5℃) cyangwa hamwe n'udupaki twa jeli kugira ngo zigumane ubushyuhe bukonje cyangwa bukonje mu gihe cyo kugenda. Niba poroteyine ihamye ku bushyuhe bukonje (2-8℃) mu gihe gito, ishobora koherezwa kuri dogere 2-8℃ hamwe n'udupaki twa jeli kugira ngo zigumane ubushyuhe bukonje mu gihe cyo kugenda. Ku maporoteyine ahamye ku bushyuhe bw'icyumba cyangwa ubushyuhe bukonje mu gihe kirekire, koherezwa ku bushyuhe busanzwe (15-25℃) bishobora kuba bishoboka. Alpha Lifetech iguha ibikoresho bya poroteyine hakurikijwe ibisabwa bikomeye.
5. Ni gute ubwiza bwa poroteyine zivanze bugenzurwa?

Ubwiza bwa poroteyine zivanze bugenzurwa binyuze mu ngamba zikomeye zo kugenzura ubuziranenge, harimo kumenya imiterere ya poroteyine, gusuzuma ubuziranenge, no gupima imikorere. Uburyo bwo gusesengura nka SDS-PAGE, HPLC, mass spectrometry, na bioassays bukoreshwa mu gusuzuma ubuziranenge n'ubuziranenge bwa poroteyine zivanze.

Ibibazo Bikunze Kubazwa Ku Ikoreshwa rya Serivisi yo Gukwirakwiza Poroteyine Zivanze

1. Ni izihe ngirabuzimafatizo zisanzwe zikoreshwa mu gutanga poroteyine zivanze?

Udukoko dusanzwe two mu bwoko bwa poroteyine dushobora kwihuza harimo bagiteri (urugero: Escherichia coli), umusemburo (urugero: Saccharomyces cerevisiae), uturemangingo tw’udukoko (urugero: Spodoptera frugiperda), n’uturemangingo tw’inyamabere (urugero: uturemangingo tw’intanga ngabo tw’Abashinwa). Buri turemangingo dufite ibyiza n’ibibi byatwo mu bijyanye n’urwego rw’imikorere ya poroteyine, impinduka nyuma yo guhinduka, no kwaguka. Alpha Lifetech ifite sisitemu enye z’ingenzi zo kwihuza kwa poroteyine ushobora guhitamo zikwiriye umushinga wawe, zikagufasha kurangiza neza umushinga.
2. Ni ibihe bintu bigomba kwitabwaho mu guhitamo uburyo bwo kugaragaza poroteyine mu buryo bwa recombinant?

Ibintu byo gusuzuma birimo ingano ya poroteyine n'ubugoranye, impinduka zifuzwa nyuma yo guhindura, urwego rw'imikorere, uburyo poroteyine ishonga, n'ibisabwa mu gutunganya. Byongeye kandi, ikiguzi, uburyo bworoshye bwo kuyikoresha, n'ibipimo ngenderwaho bishobora kugira ingaruka ku guhitamo uburyo bwo kuyikoresha.
3. Ni izihe nyungu zo gukoresha bagiteri mu gutanga poroteyine zivanze?

Bagiteri, nka Escherichia coli, zikunze gukoreshwa mu gukora poroteyine zisubiramo bitewe n'uko zikura vuba, koroshya kuzihindura, ndetse no gukora poroteyine nyinshi. Zikwiriye cyane cyane gukora poroteyine ntoya cyangwa ziciriritse zidasaba impinduka nyuma yo kuzihindura.
4. Ni izihe nyungu zo gukoresha uturemangingo tw’inyamabere mu gutanga poroteyine zihuza ibinyabuzima?

Uturemangingo tw’inyamabere, nk’uturemangingo tw’intanga ngabo tw’Abashinwa (CHO), dutanga inyungu zo gukora poroteyine zikomeye zisaba kuzingwa neza, guhindurwa nyuma yo guhindurwa, no gusohora. Sisitemu yo kugaragaza imiterere y’inyamabere ishobora gukora poroteyine zifite imiterere nk’iy’abantu kandi akenshi zikoreshwa mu gukora poroteyine zivura.
5. Ni izihe mbogamizi zimwe na zimwe zijyanye no gukora poroteyine zivanze?

Imbogamizi zirimo kugorana kwa poroteyine, kwishyira hamwe, urwego ruto rw'imikorere, uburozi ku kinyabuzima cyakiriye, n'ingorane zo kugera ku mpinduka zikwiye nyuma yo guhindura. Kunoza imiterere y'imikorere no guhitamo ibinyabuzima byakiriye bishobora gufasha gukemura izi mbogamizi.
6. Ni gute nakora isuku ya poroteyine ya recombinant?

Uburyo bwo gutunganya poroteyine buratandukanye bitewe n'imiterere ya poroteyine n'uburyo bwo kugaragaza imiterere ikoreshwa. Ubuhanga busanzwe burimo chromatography ya affinity, chromatography yo guhinduza ion, chromatography yo gukuramo ingano, n'uburyo butandukanye bwo gutunganya poroteyine (urugero, His-tag cleansing, GST-tag cleansing). Intambwe nyinshi zo gutunganya zishobora kuba ngombwa kugira ngo ubone poroteyine nziza cyane. Alpha Lifetech ifite uburyo bwinshi bwo gutunganya poroteyine kugira ngo igufashe kubona poroteyine nziza cyane.
7. Ni ubuhe buryo busanzwe bukoreshwa mu gusukura poroteyine zivanze?

Uburyo busanzwe bwo gutunganya poroteyine burimo chromatography (urugero: chromatography y’ubumwe, chromatography yo gupima ion exchange, chromatography yo gukuramo ingano), imvura, ultrafiltration, na dialysis. Buri buryo bukoresha imiterere itandukanye ya poroteyine kugira ngo bugere ku gusukurwa.
8. Nigute nahitamo uburyo bukwiye bwo gutunganya poroteyine yanjye?

Guhitamo uburyo bwo gutunganya ibintu biterwa n'ibintu bitandukanye, harimo ingano, imbaraga, uburyo bwo gushonga, n'imiterere yihariye ya poroteyine yifuzwa. Hakwiye kandi kwitabwaho urwego rwo gutunganya ibintu, urwego rw'ubuziranenge bwifuzwa, n'ikoreshwa rya poroteyine isukuye.
9. Ni gute ubuziranenge n'umusaruro bipimwa mu gihe cyo gusukura poroteyine?

Ubuziranenge n'umusaruro bikunze gupimwa hakoreshejwe uburyo bwo gusesengura nka SDS-PAGE, blotting y'iburengerazuba, spectroscopy y'ingufu za UV, na Bradford cyangwa BCA protein assays. Ubu buryo butanga amakuru y'imibare n'ubwiza ku miterere ya poroteyine isukuye.
10. Wakora iki niba poroteyine idafite ubushobozi bwo gushonga?

Guhindura imiterere y'imikurire y'ibinyabuzima byakiriye, nk'ubushyuhe, igihe cyo kwinjizwa mu mubiri, ubwinshi bw'ibitera imbaraga, n'imiterere y'ibinyabuzima, rimwe na rimwe bishobora gutuma poroteyine zipfunyika neza kandi bikongera ubushobozi bwo gushonga. Kongeramo ibirango by'imvange cyangwa ibirango by'imvange kuri poroteyine wifuza bishobora kongera ubushobozi bwo gushonga. Ibirango bikunze gukoreshwa mu guhuza poroteyine birimo glutathione S-transferase (GST), poroteyine iboha maltose (MBP), na small ubiquitin-related modifier (SUMO).

isuzuma

Umukiriya
isuzuma

Iyi poroteyine ya CD28 yari ifite ubuziranenge bwinshi kandi yagumanye imikorere ya biyoloji mu isuzuma ryacu ryo kwiyongera kwa selile T. Imiterere ya lyophilized yari yoroshye kongera kuyivugurura, kandi ibisubizo byari bihuye mu bushakashatsi bwose. Nakugira inama yo kuyisuzuma -- Poroteyine ya CD28 y'umuntu (ALP64560)

Dr. Michael Lee

Kaminuza ya Johns Hopkins

Twakoresheje iyi poroteyine yo mu bwoko bwa spike mu gusuzuma uburyo poroteyine n’ibinyabutabire bya ELISA byakoreshejwe ndetse no mu bushakashatsi ku mikoranire ya poroteyine. Poroteyine yari yapfunyitse neza kandi yagaragaje ko ifitanye isano rikomeye na ACE2. Hari ukwishyira hamwe guto kwagaragaye ku gipimo cyo hejuru, ariko muri rusange, ni umusaruro mwiza --Poroteyine ya SARS-CoV-2 Spike RBD.

Dr. Olivia Martinez

Kaminuza ya Cambridge

Poroteyine ya GFP yasubiwemo yari ifite fluorescence ikomeye kandi yakoreye neza igerageza ryo gukurikirana mu buryo bwa vitro. Ubuziranenge bwari bwinshi, nubwo uburyo bwo kubika bwasabye impinduka ntoya ukurikije ibyo twari dukeneye. Muri rusange, ni umusaruro ukoreshwa mu gukoresha fluorescence--Poroteyine ya GFP yasubiwemo.

Dr. William Kim

Kaminuza ya Kyoto

010203

Umutungo wa poroteyine Ibyerekeye

Reba Amakuru yose

Kuki twahitamo Poroteyine yacu ya Recombinant?

Ibikoresho bya poroteyine bivanze

Serivisi yo gukora poroteyine zivanze ku buryo bwihariye

Kugereranya Sisitemu yo Gusobanura Poroteyine Ivanze

Ibibazo Bikunze Kubazwa Ku Bicuruzwa Bivanze bya Poroteyine

Imishinga yacuTuragukorera byose!

Umusaruro wa Poroteyine Ishonga mu Mutwe wa E. coli

Impinduka mu miterere y'uturemangingo tw'inyamabere mu guhindura nyuma yo guhindura

Igaragaza rya poroteyine idafite utunyangingo ku poroteyine z'uburozi

1. Ni gute nasaba ibiciro ku bicuruzwa byinshi?

2. Nabona nte COA ya poroteyine ya recombinant nahawe?

3. Poroteyine ya recombinant ni iki?

4. Ni ibihe bintu byo kohereza poroteyine zivanze?

5. Ni gute ubwiza bwa poroteyine zivanze bugenzurwa?

Ibibazo Bikunze Kubazwa Ku Ikoreshwa rya Serivisi yo Gukwirakwiza Poroteyine Zivanze

1. Ni izihe ngirabuzimafatizo zisanzwe zikoreshwa mu gutanga poroteyine zivanze?

2. Ni ibihe bintu bigomba kwitabwaho mu guhitamo uburyo bwo kugaragaza poroteyine mu buryo bwa recombinant?

3. Ni izihe nyungu zo gukoresha bagiteri mu gutanga poroteyine zivanze?

4. Ni izihe nyungu zo gukoresha uturemangingo tw’inyamabere mu gutanga poroteyine zihuza ibinyabuzima?

5. Ni izihe mbogamizi zimwe na zimwe zijyanye no gukora poroteyine zivanze?

6. Ni gute nakora isuku ya poroteyine ya recombinant?

7. Ni ubuhe buryo busanzwe bukoreshwa mu gusukura poroteyine zivanze?

8. Nigute nahitamo uburyo bukwiye bwo gutunganya poroteyine yanjye?

9. Ni gute ubuziranenge n'umusaruro bipimwa mu gihe cyo gusukura poroteyine?

10. Wakora iki niba poroteyine idafite ubushobozi bwo gushonga?

Umukiriya isuzuma

Umutungo wa poroteyine Ibyerekeye

Amabwiriza Asesuye yo Gutunganya Poroteyine...

Gukurikirana Sanger vs. Gukurikirana Abakurikira mu Gihe Gikurikira (NGS)

Guhishura amayobera y'imikorere y'ibinyabuzima...

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Umukiriya
isuzuma