Kuki Hitamo Poroteyine Yacu?

Isuku ryinshi:Poroteyine zacu za recombinant zikorwa hifashishijwe ikoranabuhanga rigezweho kugirango harebwe isuku irenze kandi ikureho umwanda ushobora guhungabanya imikorere yabyo.

Gukora neza:Binyuze muburyo bunoze bwo gukora, ibicuruzwa bya poroteyine byitwa recombinant bifite umusaruro mwiza kandi birashobora kugera ku ntera nini mu bikorwa byinshi.

Ubwiza buhamye:Buri cyiciro cya poroteyine zacu za recombinant zifata ingamba zikomeye zo kugenzura ubuziranenge kugirango harebwe ireme n’imikorere ihamye, biha abashakashatsi n’abahanga ibisubizo byizewe buri gihe.

Ikiguzi-Cyiza:Twumva akamaro ko gutanga agaciro tutabangamiye ubuziranenge. Muguhitamo Alpha Lifetech, urabona uburyo bwiza bwa recombinant proteine nziza kubiciro byapiganwa, ukongera ingengo yimishinga yawe yubushakashatsi utitaye kubunyangamugayo bwubushakashatsi.

Urutonde rwa poroteyine

Ibicuruzwa bya poroteyine

Alpha Lifetech ifite proteine zirenga 2000+ za recombinant kandi ikorana neza nabakiriya ibihumbi nibihumbi byabakiriya ba entreprise kwisi yose kugirango ibahe ibisubizo. Fungura ubushobozi bwa tekinoroji ya poroteyine ya recombinant hamwe na progaramu ya proteine nziza yo mu rwego rwo hejuru. Twandikire uyu munsi kugirango wihutishe imishinga yawe hamwe na resombinant protein imvugo ya serivise n'ibicuruzwa bya poroteyine.

Poroteyine ya Recombinant Kuri Kanseri

Poroteyine ya Recombinant2-Alpha Lifetech4o5

Poroteyine ya Recombinant2-Alpha Lifetechk4z

Poroteyine ya Recombinant3-Alpha Lifetechcfl

Poroteyine ya Recombinant3-Alpha Lifetechbyj

Poroteyine ya Recombinant1-Alpha Lifetechhrb

Poroteyine ya Recombinant1-Alpha Lifetech649

01020304

Ibicuruzwa byinshi

Serivisi ishinzwe kubyara poroteyine

Mu myaka 30 ishize, habaye iterambere ryinshi mu ikoranabuhanga ryo kwerekana poroteyine za recombinant.Yamazaki Inc.itanga poroteyine enye zerekana uburyo bwo gukora poroteyine za recombinant: Bagiteri, umusemburo, ingirabuzimafatizo, na sisitemu y’inyamabere.Yamazaki Inc.itanga portfolio ya proteine za recombinant ziva mububiko butandukanye, nubwoko butandukanye, ibicuruzwa birashobora gukoreshwa mubushakashatsi no gusuzuma indwara. Poroteyine za Recombinant zirimo enzymes, ibintu bikura, cytokine, proteine za Fc reseptor, proteyine zo kugenzura indwara, hamwe na poroteyine za membrane. Izi poroteyine za recombinant zirashobora gukoreshwa mubikorwa bitandukanye, birimo umuco w'akagari, bioassay, ndetse no kwisuzumisha vitro.

Recombinant Protein Yerekana Sisitemu Kugereranya

Sisitemu yo Kugaragaza

Indwara ya bagiteri

Sisitemu

Umusemburo

Sisitemu

Baculovirus

-Gukingira

Sisitemu

Inyamaswa z’inyamabere

Sisitemu

Ibyiza

Igiciro gito

Gutwara igihe

Ikiguzi

inganda

Gukura vuba

ibyahinduwe nyuma yubusobanuro

Sobanura amateka yimiterere

Imiterere yoroshye yo guhinga

Umutekano muke

Imvugo ikora neza

guhindura nyuma yubusobanuro

Uburozi buke

Umutekano ukomeye

Ibiranga iterambere rihamye

Biroroshye guhinga no guhinduranya

Imvugo ikora neza

Ingaruka

Kwangirika kwa poroteyine

Ibibazo byuburozi

Indwara ya Endotoxine

Intungamubiri za poroteyine

Umusaruro muke

Poroteyine yo hasi itanga umusaruro muke kugirango ugaragaze poroteyine nini kandi nini cyane.

Ubushobozi buke bwo gukora poroteyine zifite uburemere buke bwa molekile; Ntabwo ikora neza mugukora proteine za membrane

Igiciro kinini

Igihe kirekire cyo gukora

Ibisabwa byumuco bigoye

Gusaba

Inyigisho zubaka

Umusaruro wa proteine zishonga, proteine nto

Umusemburo wa enzyme mu nganda

Inyigisho zikorwa

poroteyine zasohotse;

Iterambere ry'inkingo

Kuvumbura ibiyobyabwenge

Inyigisho za poroteyine-protein

Ibibazo bya poroteyine za Recombinant

Imishinga yacuTuragukorera byose!

Igishushanyo 1 ： SDS-PAGE ibisubizo byo kweza poroteyine

UMWIGISHA: Marker; Umuhanda wa 1: Rukawa; Umuhanda 2-8: Poroteyine yatunganijwe neza

Umusaruro mwinshi wa poroteyine mwinshi muri E. coli

Intego Kwiga imiterere ya proteine zishonga cyane.
Ikibazo Mu ikubitiro byagaragaye muri E. coli, hashyizweho imibiri yo gushiramo idasaba gukenera.
Igisubizo Gahunda yatunganijwe yongereye umusaruro wa poroteyine zishonga kuva kuri

Igishushanyo 2 ： SDS-PAGE ibisubizo byerekana inyamaswa z’inyamabere zerekana no kwezwa (> 90% byera)

Amagambo y’inyamabere yerekanwe nyuma yubusobanuro bwahinduwe

Intego Gukora ibizamini bikora kuri poroteyine ya glycosilated.
Ikibazo Sisitemu yambere ya E. coli hamwe numusemburo wa sisitemu yananiwe gutanga ibyukuri byahinduwe nyuma yubusobanuro (PTMs).
Igisubizo Optimisation yavuyemo ubutinganyi bwa profili isukari isabwa> 90%.

Igishushanyo 3 ： WB ibisubizo byo kweza poroteyine

Kugaragaza poroteyine zitagira selile kuri poroteyine zifite ubumara

Intego Kugaragaza poroteyine ifitanye isano na poroteyine ifite ubumara kuri selile nzima.
Ikibazo Sisitemu yo kwerekana imvugo gakondo ntabwo ikwiye.
Igisubizo Uburyo butagira selile bwatanze poroteyine zikora ku kigero cya 2-3mg / mL, zitsinda uburozi bwa sisitemu ya selile.

1.Nsaba nte gusaba amagambo yatanzwe kubicuruzwa byinshi?

Menya ibicuruzwa cyangwa ibicuruzwa wifuza kugura kubwinshi. Ibi birashobora kuba birimo poroteyine za recombinant, antibodies, cyangwa izindi reagent. Nyamuneka Twandikire kuri info@alpha-lifetech.com
2. Nabona nte COA ya proteine ya recombinant nakiriye?

Nyamuneka twandikire ukoresheje imeri info@alpha-lifetech.com kugirango umenye amakuru arambuye kubicuruzwa.
3. Poroteyine yitwa recombinant ni iki?

Poroteyine ya recombinant ni poroteyine ikorwa hifashishijwe ikoranabuhanga rya ADN ya recombinant, ubusanzwe mu kwerekana gene ishishikajwe n’ibinyabuzima byakira ibinyabuzima, nka bagiteri, umusemburo, cyangwa inyamaswa z’inyamabere. Iri koranabuhanga ryemerera gukora poroteyine zitari nyinshi cyangwa zigoye kubona aho zikomoka. Alpha Lifetech irashobora gutanga proteine za recombinant.
4. Ni ubuhe buryo bwo kohereza poroteyine za recombinant?

Poroteyine za recombinant akenshi zoherezwa ku rubura rwumye (−78.5 ℃) cyangwa hamwe nudupapuro twa gel kugirango tugumane ubushyuhe bwakonje cyangwa ubukonje mugihe cyo gutambuka.Niba poroteyine ihagaze neza mubushyuhe bwa firigo (2-8 ℃) mugihe gito, irashobora koherezwa kuri 2-8 ℃ hamwe nudupapuro twa gel kugirango tugumane ubushyuhe bukonje mugihe cyo gutambuka. birashoboka. Alpha Lifetech ikohereza ibicuruzwa bya poroteyine ukurikije ibisabwa bikomeye.
5. Ni gute ubwiza bwa poroteyine za recombinant bwubahirizwa?

Ubwiza bwa poroteyine za recombinant butangwa binyuze mu ngamba zikomeye zo kugenzura ubuziranenge, harimo kuranga poroteyine, gusuzuma isuku, no gupima imikorere. Ubuhanga bwo gusesengura nka SDS-PAGE, HPLC, sprometrike rusange, na bioassay bikoreshwa mugusuzuma ubwiza nubusugire bwa poroteyine za recombinant.

Ibibazo bya Recombinant Protein serivisi

1.Ni ubuhe bwoko busanzwe bukoreshwa mu mvugo ya poroteyine?

Ibinyabuzima bisanzwe byakira proteine ya recombinant harimo bagiteri (urugero, Escherichia coli), umusemburo (urugero, Saccharomyces cerevisiae), ingirabuzimafatizo (urugero, Spodoptera frugiperda), hamwe n’inyamabere z’inyamabere (urugero, ingirabuzimafatizo zo mu Bushinwa). Buri kinyabuzima cyakira gifite ibyiza n'ibibi mubijyanye nurwego rwa poroteyine, ibyahinduwe nyuma yubusobanuro, hamwe nubunini. Alpha Lifetech ifite sisitemu enye zingenzi zerekana imvugo kugirango uhitemo ibereye umushinga wawe, igufasha kurangiza neza umushinga.
2. Ni ibihe bintu bigomba kwitabwaho muguhitamo sisitemu yo kwerekana poroteyine?

Ibintu ugomba gusuzuma harimo ubunini bwa poroteyine nuburemere, ibyifuzo byahinduwe nyuma yubusobanuro, urwego rwimvugo, ibisubizo bya poroteyine, hamwe nibisabwa gutunganywa. Byongeye kandi, ikiguzi, koroshya imikoreshereze, hamwe nibitekerezo byigenga bishobora guhindura amahitamo ya sisitemu.
3. Ni izihe nyungu zo gukoresha bagiteri mu kwerekana poroteyine ya recombinant?

Indwara ya bagiteri, nka Escherichia coli, ikoreshwa cyane mu mvugo ya poroteyine ya recombinant bitewe no gukura kwayo vuba, koroshya imikoreshereze, hamwe na poroteyine nyinshi. Birakenewe cyane cyane kubyara poroteyine ntoya nini nini zidasaba guhindura nyuma yubusobanuro.
4. Ni izihe nyungu zo gukoresha ingirangingo z’inyamabere mu kwerekana poroteyine ya recombinant?

Ingirangingo z’inyamabere, nka selile yo mu Bushinwa hamster ovary (CHO), zitanga inyungu zo gukora poroteyine zigoye zisaba kuzunguruka neza, guhindura nyuma yubusobanuro, no gusohora. Sisitemu yo kwerekana inyamaswa z’inyamabere zirashobora gukora poroteyine zifite ishusho ya glycosylation yumuntu kandi akenshi zikoreshwa mugukora proteine zo kuvura.
5. Ni izihe mbogamizi zimwe zijyanye na proteine ya recombinant?

Inzitizi zirimo poroteyine zidahinduka, guteranya, urwego rwo hasi rwo kwerekana, uburozi ku binyabuzima byakiriye, n'ingorane zo kugera ku mpinduka zikwiye nyuma yo guhindurwa. Kunoza imiterere yimiterere no guhitamo ibinyabuzima byakiriye birashobora gufasha gukemura ibyo bibazo.
6. Nigute nshobora kweza proteine recombinant?

Uburyo bwo kweza poroteyine buratandukanye bitewe na poroteyine hamwe na sisitemu yo gukoresha. Ubuhanga busanzwe burimo chromatografi ya affinity, ion guhana chromatografiya, ingano yo gukuramo chromatografiya, hamwe nuburyo butandukanye bwuburyo bwihariye bwo kweza poroteyine (urugero, kweza tagi ye, kweza GST-tag). Intambwe nyinshi zo kweza zirashobora gukenerwa kugirango ubone proteine nziza cyane. Alpha Lifetech ifite sisitemu nyinshi zo kweza poroteyine kugirango igufashe kubona poroteyine nyinshi.
7. Ni ubuhe buryo busanzwe bukoreshwa mu kweza poroteyine ya recombinant?

Uburyo busanzwe bwo kweza poroteyine harimo chromatografiya (urugero, chromatografi ya affinity, chromatografi ya ion, ingano yo gukuramo chromatografiya), imvura, ultrafiltration, na dialyse. Buri buryo bukoresha ibintu bitandukanye byumubiri cyangwa imiti ya poroteyine kugirango bigere ku kwezwa.
8. Nigute nahitamo uburyo bukwiye bwo kweza proteine yanjye?

Guhitamo uburyo bwo kweza biterwa nibintu bitandukanye, harimo ingano, kwishyuza, gukomera, hamwe nibintu byihariye bya poroteyine. Hagomba kandi gutekereza ku gipimo cyo kwezwa, urwego rwifuzwa rwo kwezwa, hamwe nuburyo bukoreshwa bwa poroteyine yatunganijwe.
9. Ni gute isuku n'umusaruro bisuzumwa mugihe cyo kweza poroteyine?

Ubuziranenge n'umusaruro bisuzumwa hifashishijwe tekinoroji yo gusesengura nka SDS-PAGE, blotting yo mu burengerazuba, UV yinjira muri spekitroscopi, na Bradford cyangwa BCA proteine. Ubu buhanga butanga amakuru yuzuye kandi yujuje ubuziranenge bwa protein yatunganijwe.
10. Wakora iki niba poroteyine yagenewe idashobora gukemuka?

Guhindura imiterere yikura ryibinyabuzima byakiriye, nkubushyuhe, igihe cyo kwinjiza, kwibanda kwa inducer, hamwe n’umuco ugereranije, birashobora rimwe na rimwe guteza imbere poroteyine ikwiye no kongera imbaraga. Ongeraho ibirango bya fusion cyangwa solubility tags kuri proteine yawe irashobora kunoza ibisubizo. Ibiranga fusion bisanzwe birimo glutathione S-kwimura (GST), poroteyine ya maltose-ihuza (MBP), hamwe na modifier ntoya ya ubiquitin (SUMO).

gusuzuma

Umukiriya
gusuzuma

Iyi poroteyine ya CD28 yari ifite isuku nyinshi kandi ikomeza ibikorwa byibinyabuzima mubisubizo byacu bya T-selile. Imiterere ya lyofilize yari yoroshye kuyubaka, kandi ibisubizo byari bihuye mubushakashatsi. Byaba byiza rwose dusabye ubushakashatsi - Poroteyine yumuntu CD28 (ALP64560)

Dr. Michael Lee

Kaminuza ya Johns Hopkins

Twifashishije proteine ya spike kubushakashatsi bwa ELISA bushingiye ku kutabogama hamwe nubushakashatsi bwa protein-protein. Poroteyine yari ifunze neza kandi yerekana guhuza ACE2. Guteranya kworoheje byagaragaye cyane, ariko muri rusange, ibicuruzwa byiza - SARS-CoV-2 Spike RBD Protein.

Dr. Olivia Martinez

Kaminuza ya Cambridge

Poroteyine ya recombinant GFP yari ifite fluorescence ikomeye kandi ikora neza mubushakashatsi bwa vitro. Isuku yari hejuru, nubwo ububiko bwo kubika bwasabye guhindura bike kubyo dukeneye. Muri rusange, igicuruzwa cya porogaramu ya fluorescence - Poroteyine ya Recombinant.

Dr. William Kim

Kaminuza ya Kyoto

010203

Ibikoresho bya poroteyine Ibyerekeye

Reba Amakuru yose

Kuki Hitamo Poroteyine Yacu?

Ibicuruzwa bya poroteyine

Serivisi ishinzwe kubyara poroteyine

Recombinant Protein Yerekana Sisitemu Kugereranya

Ibibazo bya poroteyine za Recombinant

Imishinga yacuTuragukorera byose!

Umusaruro mwinshi wa poroteyine mwinshi muri E. coli

Amagambo y’inyamabere yerekanwe nyuma yubusobanuro bwahinduwe

Kugaragaza poroteyine zitagira selile kuri poroteyine zifite ubumara

1.Nsaba nte gusaba amagambo yatanzwe kubicuruzwa byinshi?

2. Nabona nte COA ya proteine ​​ya recombinant nakiriye?

3. Poroteyine yitwa recombinant ni iki?

4. Ni ubuhe buryo bwo kohereza poroteyine za recombinant?

5. Ni gute ubwiza bwa poroteyine za recombinant bwubahirizwa?

Ibibazo bya Recombinant Protein serivisi

1.Ni ubuhe bwoko busanzwe bukoreshwa mu mvugo ya poroteyine?

2. Ni ibihe bintu bigomba kwitabwaho muguhitamo sisitemu yo kwerekana poroteyine?

3. Ni izihe nyungu zo gukoresha bagiteri mu kwerekana poroteyine ya recombinant?

4. Ni izihe nyungu zo gukoresha ingirangingo z’inyamabere mu kwerekana poroteyine ya recombinant?

5. Ni izihe mbogamizi zimwe zijyanye na proteine ​​ya recombinant?

6. Nigute nshobora kweza proteine ​​recombinant?

7. Ni ubuhe buryo busanzwe bukoreshwa mu kweza poroteyine ya recombinant?

8. Nigute nahitamo uburyo bukwiye bwo kweza proteine ​​yanjye?

9. Ni gute isuku n'umusaruro bisuzumwa mugihe cyo kweza poroteyine?

10. Wakora iki niba poroteyine yagenewe idashobora gukemuka?

Umukiriya gusuzuma

Ibikoresho bya poroteyine Ibyerekeye

Amabwiriza yuzuye yo kweza poroteyine ...

Urutonde rwa Sanger na Urukurikirane-Ibisekuruza bizakurikiraho (NGS)

Gufungura Amayobera yuburyo bwibinyabuzima ...

Leave Your Message

2. Nabona nte COA ya proteine ya recombinant nakiriye?

5. Ni izihe mbogamizi zimwe zijyanye na proteine ya recombinant?

6. Nigute nshobora kweza proteine recombinant?

8. Nigute nahitamo uburyo bukwiye bwo kweza proteine yanjye?

Umukiriya
gusuzuma