Serivisi yo kuvumbura abarwayi ba scFv

Alpha Lifetech Inc.Ifite ishema ryo gutanga serivisi zuzuye zo kubaka isomero rya scFv kugira ngo ihuze n'ibyo abahanga mu bya siyansi ku isi bakeneye. Intego yacu ni ukumva no guhaza ibyifuzo by'abakiriya batandukanye no gufasha mu bibazo byose bizaza n'ibivuka mu bushakashatsi.

Antibody ya scFv-Umugozi umwe uhinduka igice kimwe ni iki?

Antibodies y’uruhererekane rumwe igizwe n’urumuri rw’urumuri ruhinduka (VL) n’uruhererekane ruremereye (VH) ruhujwe n’agace k’uruhererekane rw’ingirabuzimafatizo gahuza peptide, gashobora kugaragara byoroshye mu buryo bw’imikorere ku buso bwa phage bityo kazwi nka single-chain variable fragment (scFv). Agace ka scFv ni igice gito cyane cya immunoglobulin molekile (usibye nanobodies) gifite imikorere mu bikorwa byo gufata antigen kandi cyemerera poroteyine gukora kunoza imiterere ya scFv (single chain fragment variable) nko kongera ubwifatanye no guhindura umwihariko. Muri Alpha Lifetech Inc., antibodies za scFv zubatswe ahanini zivuye muri hybridoma, uturemangingo tw’uruhererekane duturutse ku mbeba zakingiwe, na B lymphocytes ziturutse ku bantu. RNA yose cyangwa mRNA ibanza gukurwa muri hybridoma, uturemangingo tw’uruhererekane, uturemangingo tw’uruhererekane, n’uturemangingo tw’umusemburo w’amagufwa hanyuma bigashyirwa muri cDNA kugira ngo bibe icyitegererezo cyo kongera gene y’abarwayi (PCR).

Ikoranabuhanga ryo kwerekana ubudahangarwa bw'umubiri bwa scFv

Ubudasa bw'isomero buterwa n'umubare w'uturemangingo twigenga. Kugira ngo hakorwe isomero rinini rya antibody ya scFv, abahanga bacu bazakoresha ingamba zacu bwite z'intambwe ebyiri zo gukora isomero. Mu ntambwe ya mbere y'uburyo bwavuzwe, repertoire z'ibanze zitegurwa zivuye ku bicuruzwa bya PCR bikubiyemo domaine za VH, Vκ, na Vλ, bigatanga isomero risanzwe riciriritse (clones miliyoni 1 - 100). Mu ntambwe ya kabiri, ibice bya VH (cyangwa VL) bikurwamo binyuze mu gusya ADN ya plasmid isukuye mu nyuguti z'ibanze hanyuma bigashyirwa muri vector ya acceptor phagemid irimo repertoire za VL (cyangwa VH). Ubu buryo bushya bwongera ingano y'amasomero cyane (10)¹⁰-10¹²clones).

Gusuzuma Antibody ya scFv mu buryo bunini kandi buhanitse

Ku bijyanye no gutegura scFv ku rwego runini, biragaragara ko guhitamo uburyo bwo gutanga ibitekerezo bizagira ingaruka zikomeye ku miterere y'imikorere, ingamba zo gutunganya ibintu, n'ikiguzi cy'imikorere. Guhitamo inzira yo gukora bizaterwa n'akamaro ko guhindura nyuma yo guhindura ibintu n'igihe cyo guteza imbere ubuvuzi n'ubukungu, ibyo nabyo ahanini bikaba bishingiye ku 'buryo bwo gutanga ibitekerezo'. Nta buryo bwo gutanga ibitekerezo rusange bwemeza urwego rwo hejuru rw'imikorere y'ibicuruzwa ku bwoko bwinshi bw'abasirikari b'umubiri cyangwa poroteyine zitandukanye.