Serivisi yo kuvumbura Antibody

Yamazaki Inc.yishimiye gutanga serivisi zuzuye zububiko bwibitabo bwa scFv kugirango zihuze ibyifuzo bitandukanye byabahanga ku isi. Intego yacu nukwumva no guhuza ibyifuzo byabakiriya batandukanye no gufasha mubibazo byose biri imbere kandi bivuka mubikorwa byubushakashatsi.

Niki scFv- Urunigi rumwe Rurwanya Ibice Antibody?

Antibody yumunyururu umwe igizwe na antibody ihindagurika yumucyo (VL) nigice kiremereye (VH) ihujwe na peptide ihuza byoroshye, ishobora kugaragazwa byoroshye muburyo bukora hejuru ya fage bityo ikaba isanzwe izwi nkigice kimwe cyumunyururu (scFv). Igice cya scFv nigice gito cya molekile ya immunoglobuline (usibye nanobodies) ifite imikorere mubikorwa bya antigen-ihuza kandi ituma ubwubatsi bwa poroteyine butezimbere imitungo ya scFv (impinduka imwe yumunyururu uhindagurika) nko kongera ubumwe no guhindura umwihariko. Muri Alpha Lifetech Inc., antibodiyite za scFv zubatswe cyane cyane zivuye muri hybridoma, ingirabuzimafatizo ziva mu mbeba zakingiwe, na lymphocytes B ziva mu bantu. RNA cyangwa mRNA yose yabanje gutandukanywa na hybridoma, spleen, lymph selile, na selile marrow selile ikurikirwa no guhindurwa muri cDNA kugirango bibe icyitegererezo cyo kongera antibody ya gene (PCR).

scFv Antibody Iterambere-Icyiciro Cyerekana Ikoranabuhanga

Ubwinshi bwibitabo bugenwa numubare wa clone yigenga. Kugirango tubyare isomero rinini rya scFv antibody, abahanga bacu bazakoresha ingamba zacu bwite zintambwe ebyiri zo kubyara amasomero. Mu ntambwe yambere yuburyo bwasobanuwe, repertoire yibanze itegurwa mubicuruzwa bya PCR bikubiyemo domeni ya VH, Vκ, na Vλ, bitanga amasomero asanzwe aciriritse (1 - 100 million clone). Mu ntambwe ya kabiri, ibice bya VH (cyangwa VL) bitandukanijwe no gusya ADN ya plazimasi yasukuwe mu ndirimbo zibanza hanyuma igashyirwa mu cyerekezo cyakira fagemide ikubiyemo repertoire za VL (cyangwa VH). Ubu bushya bwongera ubunini bwibitabo (clon 1010-1012).

Kinini-nini kandi yinjiza cyane Kugaragaza scFv Antibody

Kuburyo bunini bwo gutegura scFv, biragaragara ko guhitamo imvugo yakira bizagira ingaruka zikomeye kubishushanyo mbonera, ingamba zo gutunganya ibintu, hamwe nigiciro cyibikorwa. Guhitamo inzira yo gukora bizaterwa ningaruka zo guhindura nyuma yubusobanuro nigihe ntarengwa cyiterambere ryamavuriro nubukungu, nabyo bikaba ari 'sisitemu yo kwakira' -bishingiye. Nta sisitemu yo kugaragariza isi yose yerekana ibicuruzwa byerekana urwego rwo hejuru rwa antibodi zitandukanye cyangwa na poroteyine.