Serivisi yo kubaka isomero rya antibody rya scFv

Intangiriro ya serivisi yo kubaka isomero rya scFv Antibody

scFv ni agace gato k’uturemangingo tw’umubiri kagizwe n’urunigi ruremereye (VH) n’urunigi rworoheje (VL) ruhujwe na peptide ngufi, ifite ingano ya kDa 25, kandi ni bwo bwoko bwa antibody ikoreshwa cyane. scFv ni ntoya, yihariye, ishobora gukorwa ku rugero runini muri sisitemu yo kugaragaza prokaryotic, kandi ifite aho iboha antigen, ikoreshwa cyane mu gusuzuma no kuvura indwara. Kubera ko ari ntoya, biroroshye kwinjira mu turemangingo vuba mu kuvura indwara; byongeye kandi, scFv ifite ibyiza byo kumara igihe gito mu turemangingo tutari twibasiwe no gusukurwa vuba mu maraso, bituma igira ubushobozi bwinshi bwo gukoreshwa mu gupima kanseri. Amasomero asanzwe ya antibody ya scFv ni amasomero ya phage, byongeye kandi, ribosome, yeast cell, mammalian cells na bagiteri nabyo bishobora gukoreshwa mu kubaka amasomero ya scFv.

Alpha Lifetech Ishobora Gutanga

Alpha Lifetech ifite sisitemu yo kubaka isomero ry’abasilikari ba scFv ihamye, kandi ishobora gutegura amasomero y’abasilikari ba scFv ku turemangingo tw’udusimba tw’inyamaswa zakingiwe na lymphocyte B zikomoka ku bantu. Byongeye kandi, dusesengura amakuru yose yerekeye urukurikirane rw’abasilikari ba scFv kandi tukabyemeza dukoresheje uburyo butandukanye bwo gusuzuma, nko gusuzuma EC50, gusesengura affinity, kwemeza flow-through blocking, nibindi. Dufite urubuga rutandukanye rwa phage rurimo M13, T4, T7 na λ phage, rushobora guhaza ibyifuzo bitandukanye by’abakiriya no gutanga serivisi zihariye zo kubaka isomero rya scFv. Abashakashatsi bacu bafite uburambe bw’imyaka myinshi mu kubaka isomero kandi ni abahanga mu kubaka ubwoko butandukanye bwa somero zerekana scFv phage, nka somero z’ubudahangarwa bw’umubiri, isomero karemano, isomero ry’ubudahangarwa bw’umubiri, isomero ry’ubudahangarwa bw’umubiri nibindi. Isomero ry’abasilikari ba scFv twubatse rifite ubushobozi bunini bw’isomero kandi rifite ubwoko bwinshi bw’ubudahangarwa bw’abasilikari ba scFv bafite isano ikomeye. Twatanze serivisi zo kubaka isomero rya antibody ya scFv mu kurwanya bagiteri, virusi, uburozi, receptor na antigens za tumor, peptides, nibindi ku bigo byinshi byo mu gihugu no mu mahanga. Ikoranabuhanga ryo kwerekana antibody ya phage ryoroshye gukora, rifite igihe gito cyo kugerageza, kandi rishobora kugaragaza scFv yifuzwa ku bwinshi binyuze mu buryo bw'igaragaza ry'inyamabere. Ikoranabuhanga rya antibody ya scFv ihuza recombinant ryakozwe hakoreshejwe uburyo bwo kwerekana phage ntabwo risaba itegurwa ry'uturemangingo twa hybridoma, kandi rishobora gukorana neza na gene ya scFv muri vector ya phage wifuza.

Uburyo bwo kubaka isomero rya scFv Antibody bukoreshwa mu kubaka isomero

Dufite ubuhanga mu guhuza VH na VL hamwe na peptide yoroshye kugira ngo twubake scFv isabwa n'abakiriya bacu. Dushobora guha abakiriya bacu ubwoko bwinshi bwa vectors za phage zirimo pMECS, pComb3X na pCANTAB 5E. Dufite ubwoko nka TG1 E. coli, XL1-Blue na ER2738, bushobora gukoreshwa mu gutera phage nyuma yo kwaguka. Twubatse amasomero ya antibody afite ubushobozi bunini bwo kugera kuri 10^9, afite ubwoko bwinshi kandi afite igipimo cyo gushyiramo ibice byinshi, bituma hapimwa antibody za scFv zifite ubushobozi bwinshi kandi zihariye kugira ngo abakiriya banyurwe. Dushobora kandi kugaragaza no gusukura scFv yasuzumwe hakurikijwe ibyo abakiriya bakeneye. Uretse sisitemu yo kugaragaza prokaryotic, dufite kandi sisitemu zitandukanye zo kugaragaza eukaryotic kuri poroteyine za antibody, nka selile y'inyamabere, selile y'umusemburo, sisitemu yo kugaragaza uturemangingo tw'ibimera n'udukoko, nibindi, bidufasha gukora antibody nziza za scFv ku bakiriya bacu. Alpha Lifetech izatanga serivisi zuzuye zo kubaka isomero rya scFv kugira ngo ihuze n'ibyo abahanga mu bya siyansi bakeneye hirya no hino ku isi bakeneye.

Imikorere ya serivisi yo kubaka isomero rya antibody rya scFv