Serivisi yo kubaka umurongo uhamye

Alpha Lifetech imaze imyaka myinshi ikora cyane mubikorwa byiterambere ryimikorere ya selile. Yubatsemo uburyo bwiza bwo kubaka umurongo utunganijwe neza, hamwe nubwoko burenga 300 bwingirangingo yibibyimba hamwe na selile yibanze, bishobora gutanga imirongo ihamye mugihe gikoresha igihe cyubushakashatsi bwa siyansi cyangwa ubushakashatsi bwumushinga, no koroshya umusaruro. Alpha Lifetech irashobora kandi guha abakiriya serivisi nko guteza imbere inzira zitoroshye, kugenzura ibibazo, kubaka imiterere, proteine recombinant, no gukora antibody, bitanga ishingiro ryubushakashatsi kumiti mishya.

Alpha Lifetech ifite urubuga rwanduye rwa lentivirus, rushobora guhitamo uburyo bwubushakashatsi bwubwoko butandukanye bwimikorere ihindagurika. Twifashishije tekinoroji ya gene knockout kugirango twubake imirongo ya selile CRISPR / Cas9. Gukabya gukabije kwa gene hamwe nubuhanga bwo gucecekesha gene byakoreshejwe muguhindura imiyoboro yimikorere. Urubuga rwihariye rwuzuzanya rwuzuza vertex guhuza ibikorwa byumurongo wubwubatsi.

Intangiriro yo kubaka umurongo uhamye

Umurongo uhamye wa selile werekana umurongo wa selile uhuza genes zamahanga muma genoside yakira kugirango genes zamahanga zigaragare neza muri selile yakiriye igihe kirekire. Ihame ni ukugabanya gene yamahanga kuri vector hamwe nuburwanya runaka, guhinduranya selile zakira, kwinjiza gene yamahanga muri chromosome yakira, no kwerekana genes zo kurwanya zikubiye muri vector kugirango zibone imirongo ihamye hamwe na proteine igamije cyangwa gucecekesha byimazeyo gene yagenewe yujuje ibisabwa nubushakashatsi.

Alpha Lifetech ifite amahugurwa yigenga yo kubika, sisitemu nziza yo gukonjesha no kubika ububiko, hamwe nubwoko burenga 300 bwingirabuzimafatizo hamwe na selile yibanze kugirango uhitemo, byeguriwe ubushakashatsi bwawe bwa siyansi.

Inzira yo kubaka umurongo uhamye

Uburyo bwo kubaka umurongo uhamye wubaka

Uburyo buhamye bwo kubaka umurongo wa selile burimo lentivirus-yunganirwa, transposon-yunganirwa, CRISPR / Cas9-yunganirwa, hamwe no kwerekana plasmid.

lentivirus-yunganirwa nuburyo bumwe bukoreshwa muburyo bwo kubaka imirongo ihamye. Ubu buryo bufite inyungu zo kwanduza ubwoko bwose bwutugingo ngengabuzima no kwinjiza ibikoresho bya genoside muri selile yakira kugirango bigaragare neza mugihe kirekire. Nyamara, iyi sisitemu ifite aho igarukira, kandi lentivirus ifite ubushobozi buke bwo gupakira, ntabwo rero ikwiriye kwandukura genes zifite uturere twinshi two kwandukura.

Ugereranije na lentivirus-yunganirwa, transposon-yunganirwa nta mbogamizi ku bunini bwa gene, kandi hamwe nogukomeza gutezimbere ikoranabuhanga, imikorere yo kwinjiza transposon yatejwe imbere inshuro nyinshi. Ikibi ni uko ibibujijwe bimwe na bimwe byingirabuzimafatizo na selile bigoye kwandura.

CRISPR / Cas9-yunganirwa irimo crispr gene knockout na crispr gene knockin. Crispr gene knockout bivuga kwinjiza ADN ibice bibiri, bikaviramo gutakaza imikorere ya gen. Crispr gene knockin bivuga ibice bya ADN byo mu mahanga bigizwe na genes zihariye, zishobora gukoreshwa mu gusana ingirabuzima fatizo cyangwa kumenyekanisha ingirabuzimafatizo nshya.

Isuzuma rya plasmid nigikorwa cyambere cyimyubakire yumurongo wubaka, gikenera gusa guhinduranya neza intego ya plasmid muri selire no gukoresha imyigaragambyo ijyanye no kwerekana igihe kirekire. Noneho amahirwe runaka yo kubona imirongo ihamye.

Uburyo bwo kubaka imirongo itandukanye ihamye

Uburyo bwo Guhuza	Sisitemu ya Lentivirus	Sisitemu ya Transposon	CRISP / Cas9-yunganirwa	Kugaragaza Plasmid
Ubushobozi bw'abatwara	5 kb	10 kb	5 kb	10 kb
Gukora neza	Hejuru	Bisanzwe	Bisanzwe	Hasi
Igihe ntarengwa	Ibyumweru 1-2	Ibyumweru 3-4	Ibyumweru 3-4	Ibyumweru 4-6
Ibyiza	Byihuse kandi neza	Umutekano n'umutekano	Guhuza ingingo zihamye	Igiciro gito
Ikibazo	Ibyago byo kubungabunga umutekano	Kugaragaza ubwinshi bisanzwe	CAS9 transduction iragoye kandi ihenze	Gukora neza no guhungabana

Ingorane za tekinike mukubaka imirongo ihamye

Mbere yo kwagura ibikorwa, hakoreshwa ibinyabuzima bito kugira ngo hamenyekane ibipimo nka gaze, sisitemu y’amazi y’umuco, imbaraga za hydrodynamic ntoya ikoreshwa mu tugari, nibindi, kugirango umuco wambukiranya imipaka. Nubwo, nubwo ibipimo bihuye nibikorwa byagenwe, ibidukikije bya bioreactors nini muri producer bizaba bitaringaniye, kandi biroroshye kunanirwa. Alpha Lifetech ifite uburambe bwimyaka myinshi mubikorwa byo kwagura umuco, bishobora kwirinda amakosa kurwego runaka, kugabanya ingaruka, no kuzamura cyane intsinzi. Muri icyo gihe, Alpha Lifetech yemeza ko ibikoresho fatizo hamwe n’ibitangazamakuru byakoreshejwe, bikagabanya umuvuduko wo kunanirwa kwimikorere yimikorere yimikorere.

Alpha Lifetech ifite urubuga rwimikorere rwimikorere itunganijwe kugirango iteze imbere umurongo wimikorere mugihe cyo gukora. Turashobora kandi kuguha serivisi yihariye kugirango uhuze ibyo ukeneye.

Imirongo ihamye ya selire yububiko Serivisi yo kubaka

Ibirimo	Igihe ntarengwa
Umuco HEK293 umurongo utugari utangwa nabakiriya.	3-5 amezi
Kubaka plasmid ya sgRNA-Cas9 (hamwe na gene G418).
SgRNA-Cas9 no gusana inyandikorugero ya ADN yafatanyirijwe hamwe kumurongo wa selire HEK293.
Kugenzura umurongo wa selile ya Monoclonal (GFP gene knockin nziza clone).
Gutanga: Vector, GFP gene gukomanga, ibisubizo bikurikirana, amakuru yose yibanze, raporo ya laboratoire

Ingirabuzimafatizo zirashobora gukurikiranwa

Ingirabuzimafatizo zaguzwe mumashyirahamwe yabigize umwuga nka ATCC kandi arashobora gutanga gihamya yujuje ibyifuzo byawe byo kwiyandikisha.

Inyungu y'umusaruro

Alpha Lifetech ifite uburambe bwimyaka myinshi kugirango ihuze ibyifuzo bitandukanye byiterambere; Dufite selile zitandukanye, amahugurwa yigenga yo kubika, gukonjesha selile neza, hamwe na sisitemu yo kubika.

umushinga Kugenzura ubuziranenge

Buri mushinga uhura nuburyo bunoze bwo kugenzura ubuziranenge kugirango harebwe itangwa ryimirongo ngari yujuje ubuziranenge kubakiriya.

Inyungu ya tekiniki

Usibye abajenjeri bafite uburambe buke bwa selile, dufite tekinoroji ya tekinoroji ya algorithms yo kunoza imiterere ya reaction no guhindura ibipimo byimikorere.

Niba ufite ikibazo, nyamuneka twandikire igihe icyo aricyo cyose.

Leave Your Message

Serivisi yihariye

0102