Intangiriro ku isomero ry'amatsinda y'abarwanyi bakoreweho ubushakashatsi

Isomero ry’abarwanya indwara za synthesized, rizwi kandi nka De novo Library, ni uburyo bwo gukora ibintu bukoresha ubuhanga nka synthesis ya ADN cyangwa phage display kugira ngo hakorwe kandi hakorwe uturere tw’abarwanya indwara twagutse, harimo uturere twa framework na CDR, hatishingiwe ku masuzumiro y’abarwanya indwara za naïve.

Isomero ry’uturemangingo tw’umubiri dukozwe hakoreshejwe guhuza amasomero y’uturemangingo tw’umubiri dusanzwe hamwe n’ubwoko butandukanye bw’uturemangingo. Akenshi bikubiyemo gukora oligonucleotides za ADN kugira ngo habeho ubwoko butandukanye bw’uturere tw’uturemangingo twuzuzanya (CDR), hanyuma bigahuzwa n’imiterere y’uturemangingo tw’umubiri duhoraho dukomoka ku bintu karemano nk’uturemangingo twa B tw’abantu cyangwa inyamaswa. CDR y’uturemangingo ishyira ubwoko butandukanye mu isomero, bigatuma uturemangingo twibasira ubwoko bwinshi bw’uturemangingo tw’umubiri. Isomero ry’uturemangingo tw’umubiri dusanzwe ritanga uburyo bwo guhuza ubwoko karemano bw’uturemangingo tw’umubiri n’ubwoko butandukanye bugenzurwa bugerwaho binyuze mu buryo bw’uturemangingo.

Itandukaniro riri hagati y’amasomero y’uturemangingo tw’umubiri tudahuje n’uturemangingo tw’umubiri dushingiye ku isoko y’uturemangingo tw’umubiri dushingiye ku ngirabuzimafatizo za immunoglobulin. Hagati aho, itandukaniro riri hagati y’isomero ry’uturemangingo tw’umubiri dushingiye ku buryo busesuye n’utw’umubiri dushingiye ku buryo busesuye riboneka mu gice cy’uturemangingo tw’umubiri tudahuje n’uturemangingo, nk’uruhererekane rworoheje cyangwa uruhererekane ruremereye, kandi kimwe muri byo ni cyo cyakorewe muri vitro; mu gihe icyakozwe gikomoka ku buryo bw’ubukorano bwa PCR muri vitro.

Alpha Lifetech ishobora gutanga

Alpha Lifetech Inc.ishobora gutanga isomero ry’uturemangingo tw’umubiri dukozwe mu buryo buciriritse/bukozwe mu buryo bwa “semi-synthesized” duturutse ku nyamaswa n’abantu hashingiwe ku rubuga rwacu rw’umwuga rwo kuvumbura uturemangingo. Hamwe n’uburambe bw’imyaka myinshi mu guhindura imiterere y’uturemangingo no kubaka isomero, amasomero y’uturemangingo ashobora kubakwa ashobora gukora uturemangingo tw’umubiri dufite ubushobozi n’umwihariko birenze ubushobozi bw’uturemangingo karemano.Alpha Lifetech'sImpuguke zishimiye gutangaza ko dushobora kwemeza ko tuzagira umusaruro mwinshi mu kubaka isomero ry’abashinzwe kurwanya indwara ririmo udukoko twigenga 10^8 - 10^10.

Alpha Lifetech Inc.Ifite ishema ryo gutanga serivisi zuzuye zo kubaka isomero ry’abarwayi barinda indwara kugira ngo ihuze n’ibyo abashakashatsi ku isi bakeneye, harimo scFv, Fab, VHH antibody, n’amasomero yihariye. Intego yacu ni ukumva no guhaza ibyifuzo by’abakiriya batandukanye no gufasha mu bibazo byose bizaza n’ibivuka mu bushakashatsi.

Uburyo bwo kubaka amasomero y’abarwanyi bakoreweho ubushakashatsi

Igishushanyo
Icyiciro cyo gushushanya kirimo guhitamo imiterere y’uturemangingo tw’umubiri n’uturere tugena ubwuzuzanye (CDRs) hashingiwe ku miterere y’uturemangingo tw’umubiri cyangwa amakuru y’imiterere y’uturemangingo. Uturemangingo tw’umubiri twa CDR dushobora kandi gutegurwa kugira ngo dushyireho imikorere yihariye cyangwa twongere imiterere yo gufatana.

Gukora sintesi
ADN y’ubukorano ikora urutonde rw’uturemangingo tw’umubiri, harimo uturere twombi tw’imiterere y’umubiri na CDR, ikorwa hakoreshejwe uburyo bwa chimique cyangwa enzyme.

Inteko
Uduce twa ADN twakozwe mu buryo bwa "synthetic ADN" dushyirwa hamwe mu turemangingo tw’uturemangingo tw’umubiri hakoreshejwe uburyo nka PCR, "ligation", cyangwa "Gibson assembling". Utwo turemangingo dushobora gushyirwa mu turemangingo tw’uturemangingo nk’uturemangingo twa bagiteri, "yeast", cyangwa "mammaries" kugira ngo hakorwe "antibody".

Gusuzuma no Guhitamo
Amasomero y’abasilikare bubatswe arasuzumwa kandi agatoranywamo abasilikare bafite imiterere ikenewe hakoreshejwe uburyo bwo gusuzuma bukoresha uburyo bwo hejuru. Ibi bishobora kuba birimo ubuhanga nko kwerekana phage, kwerekana yeast, cyangwa kwerekana ribosome, bitewe n’imiterere y’isomero n’ikoreshwa ryaryo.

Serivisi yo kubaka amasomero arwanya indwara zishingiye ku binyabuzima

Alpha Lifetech Inc.Iha abakiriya igishushanyo mbonera kimwe na serivisi yo guhuza amasomero y’abasilikare b’umubiri binyuze mu gushyira repertoires z’uruhererekane ruremereye kandi rworoheje rwa V-gene muri vector ya phage ikoresheje sisitemu ya Cre-lox yo gusubiramo kugira ngo habeho repertoire ya Fabs igaragara kuri phage igizwe na clones 6.5 × 10^10. Isomero ryatanze Abs irwanya antigen nyinshi, zimwe zifite nanomolar affinities. Ikorwa ry’isomero ry’abasilikare b’umubiri rishingiye ku ikoranabuhanga ryo kwerekana M13 phage (rigaragara ku ishusho iri hepfo).