Intangiriro Kumasomero ya Antibody

Isomero rya antibody isomero, izwi kandi nk'isomero rya De novo, ni uburyo bwogukoresha bukoresha tekinike nka synthesis ya ADN cyangwa fage yerekanwe mugushushanya no guhuza uturere twuzuye twa antibody, harimo uturere twa CDR, udashingiye kumasomero ya antibody ya naïve.

Isomero rya antibody ya Semi-synthique yakozwe muguhuza amasomero asanzwe ya antibody hamwe nibitandukanye. Mubisanzwe bikubiyemo guhuza ADN oligonucleotide kugirango ikore ibice bitandukanye byuzuzanya bigena uturere (CDR), hanyuma bigahuzwa hamwe na antibody ihoraho ikomoka kumasoko karemano nka selile yabantu cyangwa inyamaswa B. CDR ikomatanya itangiza ubudasa mubitabo, bikavamo antibodies yibasira epitopes zitandukanye. Isomero rya antibody ya kimwe cya kabiri itanga ubwumvikane hagati yubwoko butandukanye bwimikorere yumubiri hamwe nubudasa bugenzurwa bwagezweho hakoreshejwe uburyo bwa sintetike.

Itandukaniro riri hagati yisomero rya antibody na sintetike ya antibody biterwa ninkomoko ya genes immunoglobuline. Hagati aho, itandukaniro riri hagati yisomero rya antibody ya synthèse na synthèse antibody iri muburyo iyambere igizwe nigice cya antibody ya naïve antibody, nkurunigi rwumucyo cyangwa urunigi ruremereye, kandi imwe murimwe gusa ikomatanyirizwa muri vitro; mugihe synthèse ikomoka rwose muburyo bwa artificiel na PCR muri vitro.

Alpha Lifetech irashobora gutanga

Yamazaki Inc.Irashobora gutanga igice cya kabiri cyogusohora / guhuza isomero rya antibody ivuye mubikoko n'abantu ukurikije urubuga rwacu rwo kuvumbura antibody. Hamwe nuburambe bwimyaka muguhindura genetike no kubaka isomero, amasomero ya synthesize arashobora kubakwa ashoboye gukora antibodiyite ya synthique ifitanye isano kandi yihariye irenze ubushobozi bwa antibodi karemano.Alpha Lifetech'sabahanga bishimiye gutangaza ko dushobora kwemeza ko tuzatsinda cyane mukubaka isomero rya antibody ririmo 10 ^ 8 - 10 ^ 10 clone yigenga.

Yamazaki Inc.yishimiye gutanga serivisi zuzuye zububiko bwibitabo bwa antibody kugirango zihuze ibyifuzo bitandukanye byabashakashatsi ku isi, harimo scFv, Fab, antibody ya VHH, hamwe nibitabo byabigenewe. Intego yacu nukwumva no guhuza ibyifuzo byabakiriya batandukanye no gufasha mubibazo byose biri imbere kandi bivuka mubikorwa byubushakashatsi.

Synthesized Antibody Amasomero yububiko bwa serivisi yo kubaka

Igishushanyo
Icyiciro cyo gushushanya gikubiyemo guhitamo antibody hamwe no kugena uturere (CDRs) dushingiye kuri antibody ihari cyangwa amakuru yimiterere. Antibodiyite ya CDR yubukorikori irashobora kandi gushirwaho kugirango itangire imikorere yihariye cyangwa izamura imiterere ihuza.

Synthesis
ADN ya sintetike ikubiyemo urutonde rwa antibody rwateguwe, harimo uturere twombi hamwe na CDR, ikomatanyirizwa hamwe hakoreshejwe uburyo bwa chimique cyangwa enzymatique.

Inteko
Ibice bya ADN byakomatanyirijwe hamwe bikusanyirizwa muri antibody imvugo ikoresheje tekinoroji nka PCR, ligation, cyangwa inteko ya Gibson. Izi vectors zirashobora kwinjizwa muri sisitemu yo kwerekana nka bagiteri, umusemburo, cyangwa inyamaswa z’inyamabere kugirango zivemo antibody.

Kugaragaza no Guhitamo
Isomero ryubatswe rya antibody ryerekanwe kandi ryatoranijwe kuri antibodies zifite imitungo wifuza ukoresheje uburyo-bwo kwinjiza ibintu byinshi. Ibi birashobora kuba bikubiyemo tekinike nka fage yerekana, umusemburo werekana, cyangwa kwerekana ribosome, ukurikije imiterere yububiko nibisabwa.

Serivisi ishinzwe kubaka amasomero ya Antibody

Yamazaki Inc.iha abakiriya igishushanyo mbonera kimwe hamwe na serivise yububiko bwibitabo bya antibody muguhuza urunigi ruremereye kandi rworoheje V-gene repertoire mugice cya fage ukoresheje sisitemu ya Cre-lox yihariye yo kwiyubaka kugirango ikore repertoire ya Fabs yerekanwe kuri fage igizwe na clon 6.5 × 10 ^ 10. Isomero ryatanze Abs kurwanya antigene nyinshi, zimwe zifite nanomolar. Igisekuru cyibitabo bya antibody byabantu bishingiye kuri tekinoroji ya M13 yerekana (yerekanwe ku gishushanyo gikurikira).