Serivise ya VHH Antibody

Yamazaki Inc.irashobora gutanga umusaruro wihuse wa antibody imwe (antibody VHH). Ibyiciro byerekana tekinoroji byemerera abahanga kwerekana ibyo basabwa kugirango bagaragaze kandi babiranga.

Alpha Lifetech Irashobora gutanga

VHH Immune yububiko bwububiko no kwerekana

Abashakashatsi bo muri Alpha Lifetech Inc. bafite uburambe bunini mu kubaka no kwerekana antibodiyite za recombinant zishingiye ku rubuga rwacu rwerekana ibyiciro. Isomero ryakingiwe isomero rimwe rya antibody rikwiranye no kubyara cyane hamwe na antigen yihariye ya antigen ya VHH cyangwa NAR V, bityo ukirinda gufata igihe muri vitro antibody yo gukomera. Hano, dukora antigen yihariye idasanzwe ya antibody isomero kubakiriya bacu kuva alpacas ikingiwe, ingamiya, llamasi cyangwa sharke, ndetse nabantu (selile PBL ikorwa na antigene muri vitro). Mugihe cyo guhindura transcript hamwe na polymerase urunani, isomero rya antibodi imwe imwe irimo cloni miliyoni 10-100.

VHH syntheic yububiko bwububiko no kwerekana

Isomero rya sintetike imwe-isomero ya antibody isomero akenshi ryatejwe imbere na CDR1 na CDR3 ya naïve VHH cyangwa VNAR. Isomero rya antibody ya sintetike ni mubisobanuro isomero rya naïve kandi rifite ubunini buke bwa 3x10 ^ 10 VHHs zitanga ubudasa bushimishije. Ntabwo ari naïve rero ntibisaba gukingirwa llama cyangwa shark. Ibi bidufasha kubika umwanya munini kugirango tubone antibody yihariye kandi itume bishoboka guhitamo antibody kuri proteine zidafite ubudahangarwa cyangwa zabitswe. Amasomero nkaya yubukorikori yerekana isoko nziza ya antibodiyite imwe irwanya antigene, idafite immunogenic, na toxic antigens kuva amasomero ubusanzwe ari manini kandi atandukanye. Mugihe guhitamo antibodiyite nyinshi zishonga cyane-biteye impungenge, twashizeho sisitemu yo mu bwoko bwa vector phagemid vector, harimo na fagemide nyinshi, ishobora kwerekana antibodi imwe imwe muri E. coli (umusemburo) cyangwa ikayerekana hejuru yibice bya fage.

Kuki Duhitamo

Turashobora gutanga serivisi zuzuye zijyanye na nanobody, harimo gukingira inyamaswa (harimo alpacas, ingamiya, nibindi), kubaka isomero rya VHH no kwerekana, kwerekana antibody ya VHH no kwemeza, VHH antibody ya muntu, nibindi.

Gukingira inyamaswa

Dufite ibirindiro byo gukingira inyamaswa hamwe n’inyamaswa zitandukanye tugomba guhitamo, harimo ingamiya, alpaka, n'ingamiya, bifite amateka asobanutse y’inkomoko.

Intego nyinshi

Dutanga serivisi nyinshi zo kuvumbura antibody, harimo proteyine, peptide, molekile nto, virusi, proteine membrane, mRNA, nibindi byinshi.

Ubushobozi bw'isomero rikuru

Ubushobozi bw'isomero rya VHH bushobora kugera kuri 10 ^ 8-10 ^ 9, kandi igipimo cyo kwinjiza kiri hejuru ya 90%. Ubusabane bwa antibodies zerekanwe mubisanzwe muri nM kurwego rwa pM

Ikoranabuhanga ridahitamo

Tekinoroji ebyiri zingenzi ziraboneka muguhitamo: tekinoroji yo kwerekana fage hamwe na tekinoroji yerekana

Niki VHH Antibody imwe?

Antibody imwe-imwe (sdAb), cyangwa nanobody, VHH, ni igice cyurwego rwibice bya antibody ya recombinant, byerekana antibody ntoya byagaragaye ko ifite akamaro kanini. Hamwe n'uburemere bwa molekuline ya 12-15 kDa, antibodies imwe-imwe, igizwe gusa numuyoboro umwe uremereye uhinduranya urwego hamwe na injeniyeri kuva muri monomeric variable domaine yaba ingamiya ikomeye ya antibody (VHH) cyangwa amafi ya karitsiye 'IgNAR (VNAR) nta gutakaza ubushobozi bwa antigen.

nanobody-Alpha Lifetech

Antibodiyumu imwe-imwe ifite ibyiza byinshi: Guhuza cyane na antibodi ntoya, ibintu byiza bya fiziki-chimique, hamwe nubuhanga bworoshye bwo hasi. Ibiranga domaine imwe hamwe no koroshya gene manipulation ya antibody imwe ya domaine ituma bikwiranye no gukura kwinshi muri vitro.

Kugereranya hagati ya VHH ya antibody imwe ya antibody na antibody isanzwe

	Antibody imwe	Antibody isanzwe
Ingano	Ntoya, gusa urwego rumwe ruremereye (V._H.H) ~ 13 kDa	Kinini, byombi biremereye kandi byoroshye ~ 120-150 kDa
Urubuga rwa Antigen	Monomeric V._H.H subunit isabwa guhuza antigen	Iminyururu yombi isabwa kugirango antigen ihuze kandi itajegajega
Hasi	Nibyiza cyane kumurongo wo hasi	Ugereranije ni tekinoroji yoroheje bitewe nuburyo bugoye
Igihagararo	Igumana ituze n'imbaraga kuri pH ikabije n'ubushyuhe	Ntushobora kwihanganira pH ikabije cyangwa ubushyuhe
Inzira y'ubuyobozi	Inzira nyinshi z'ubuyobozi	Gucungwa hakoreshejwe inshinge, ntibishobora gutangwa kumanwa
Umusaruro	Biroroshye gukora mumasemburo cyangwa mikorobe	Ingorabahizi kandi ihenze gukora

VHH kuvumbura antibody videwo

Alpha Lifetech Irashobora Gutanga VHH Antibody Yavumbuye

VHH Antibody Yavumbuwe

Saba UMURIMO

Hano hari serivisi zijyanye na VHH Antibody Discovery. Hitamo ibyo ukeneye: serivisi ibereye kuri wewe

Ingamiya VHH Antibody
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Alpaca VHH Antibody
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LIama VHH Antibody
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Nanobody affinity gukura-Alpha Lifetech4s4

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Serivisi yo gukura

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nanobody ubushakashatsi bukora-Alpha Lifetechyfv

VHH Antibody Imikorere
Serivisi y'Ubushakashatsi

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Serivisi ishinzwe ubumuntu

VHH Isomero rya Antibody
Serivisi yo kubaka

VHH Kweza Antibody
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VHH Isomero rya Antibody
Serivisi yo Kugenzura

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SERIVISI ZINDI

Ibibazo ◢

Ikibazo.
Antibody ya VHH ni iki?

A.
Antibody ya VHH ni antibody isanzwe ibura urumuri (VL) iboneka muri serumu yinyamaswa zingamiya. Imiterere ya antibody ya VHH iroroshye kandi igizwe n'iminyururu ibiri iremereye (VH). Nanobodies igizwe numurongo uremereye uhinduranya akarere (VHH) hamwe nuburemere bwa molekuline bungana na 15 kDa. Antibody ya VHH ifite ubushobozi busanzwe bwo kumenya antigene kandi ikerekana isano ihebuje, umwihariko mwiza, ituze ryiza, hamwe no kwinjira cyane. Antibodies kumiterere yabonetse kuva mubindi bikoko, nka alpacas na sharke. Nanobodies, ikubiyemo akarere kamwe gusa gahindura urunigi ruhindagurika hamwe nuturere tubiri dusanzwe twa CH2 na CH3, nubwo tudafite domaine ya VL, ni antibody ihamye cyane hamwe na antibody ntoya izwi cyane ihuza ibikorwa bya antibody. Alpha Lifetech irashobora guha abakiriya harimo gutegura nanobody, kubaka isomero rya nanobody no kwerekana, imvugo ya VHH nanobody, hamwe nuruhererekane rwa serivisi, binyuze muri nanobody synthesis hamwe na paje yerekana iterambere ryiterambere, irashobora kubaka neza no kwerekana harimo ubwoko butandukanye bwububiko bwibitabo bwa antibody, butanga abakiriya bacu muburyo bwihariye, bwihariye bwibisubizo bya antibody. Turashobora kandi gushyiraho ingamba zokoresha antibody zumwuga kubakiriya bacu, zishobora kuba umuntu kuri nanobodies, kandi isosiyete yacu ihwanye na antibodi zabantu.
Ikibazo.
Nibihe bintu nyamukuru bya serivisi yo kuvumbura antibody ya VHH?

A.
Ubwa mbere, alpaca nta ndwara iyo ari yo yose, ituma immunogène itera imbaraga z'umubiri kugira ngo itange ubudahangarwa bw'umubiri, kandi izo nyamaswa zirashobora gukora antibodiyumu imwe. Hanyuma, twatandukanije selile imwe ya B muri alpaca PBMC, hanyuma RNA ivuye muri selile B ikuramo hanyuma yandikwa muri cDNA. Amaherezo yarangije gukoreshwa nkicyitegererezo hanyuma yerekana intego yacu VHH ikurikiranye na electrophoreis. Ibikurikira, twakoze isesengura ryikurikiranya ryerekanwe rya VHH antibody ikurikiranye, ishobora kunoza ituze hamwe nubusabane bwa antibody ya VHH yateguwe. Mugihe kimwe, turashobora kandi kuyobora site-iyobora mutagenezesi no kugenzura ituze rya antibodies za VHH. Turashobora kubona ituze ryinshi rya VHH antibody ihinduka mugutangiza uburyo bwihariye nka mutation yihariye yo gusuzuma. Injeniyeri yahise yinjiza VHH ikurikiranye muburyo bukwiye bwo kwerekana imvugo (nka plasima, virusi, nibindi) kugirango imvugo ya antibody ya VHH. Hanyuma, hifashishijwe tekinoroji yo kwerekana ibyiciro cyangwa ikoranabuhanga ryerekana imisemburo, antibodiyite za VHH zigaragarira hejuru yabakiriye, hanyuma nanoantibodies zishobora guhuza antigen igenewe guhitamo hakoreshejwe antigene zifunze na ELISA. Alpha Lifetech nayo yakurikiranye kandi igenzura nanobodies zatoranijwe kugirango hamenyekane neza nibikorwa byimikorere ya nanobody ikurikiranwa kubakiriya.
Ikibazo.
Inyungu za serivisi zo kuvumbura antibody ya VHH?

A.
Ikoranabuhanga rya VHH ryifashisha iterambere ritandukanye mu buhanga bw’ubuvanganzo, ku buryo gene igenewe ihindurwamo kandi igahindurwa hamwe mu bihe bisanzwe, ishobora gutanga igikoresho gihuza cyane n’ikoranabuhanga rindi, rishobora kuba ririmo tagi zitandukanye, kandi antibodies zakozwe n’ikoranabuhanga rya VHH zorohereza kwezwa. Ukoresheje tekinoroji yo gusuzuma cyane, Alpha Lifetech irashobora kwerekana antibodies za VHH hamwe nibikorwa byihariye bisabwa nabakiriya kuva umubare munini wa antibodi mugihe gito. Hagati aho, antibodiyite za VHH zirashobora gukorwa igihe kitazwi nubukungu, mugihe antibodiyite za VHH zihuye nubushyuhe bwinshi nizindi mashanyarazi. Antibodiyite za VHH zirashobora gukoreshwa muburyo bwa genetike kugirango habeho ubundi buryo bukoreshwa, nko guhinduka scafolds, kuranga, no guhindura aside amine yihariye. Antibodiyite za VHH zirakwiriye kurubuga urwo arirwo rwose rusanzwe ukoresheje antibodi zisanzwe, nka plaque ya microtiter, biosensor ya electrochemic, hamwe nibikoresho byinjira. Bitewe nubunini buke bwa antibodiyite za VHH zirashobora kugira ubucucike buri hejuru murwego rwo guhuza, nka antibodiyite za VHH zifite inyungu zidasanzwe mukwongera ibimenyetso bityo rero bikabije. Muri icyo gihe, antibody ya VHH ifite uburyo bwagutse cyane mugupima no kuvura ibibyimba, gusuzuma indwara, kuvura indwara zifata imyanya ndangagitsina, hamwe nizindi nzego. Antibodiyite za VHH zifite akamaro kanini mugukurikirana mycotoxine mubiribwa no kugaburira kuko byakozwe mubwoko bwa genetike kandi bifite ituze ryiza.
Ikibazo.
Nigute dushobora guhitamo immunogène?

A.
Muburyo bwo gukingira, dukoresha immunogène zitandukanye. Dukurikije imiterere ya immunogène, turashobora kuba muri antigene karemano, antombens recombinant, antigène sintetike, na antigene nto ya molekile. Antigene karemano zirimo immunogène virusi. Igikorwa cyo kweza immunogène karemano kiragoye, nacyo kiragoye kuruta izindi immunogène, kandi ikiguzi cyo kweza nacyo kiri hejuru. Immunogen ya virusi igabanyijemo urukingo rwose rukingiwe urukingo, urukingo rwa subunit, urukingo rwa virusi, na rukingo rwa mRNA. Urukingo rwose rudakorewe virusi rushobora gukangurira umubiri gutanga ubudahangarwa bw'umubiri, ariko virusi igomba kuba idakozwe rwose, kandi inzira yo kuyitegura iragoye. Ku rukingo rwa subunit, twakoresheje gusa poroteyine yo hejuru ya virusi nka antigen, bityo umutekano w’iyi immunogen ukaba mwinshi, ariko ibikenewe byongerwaho kugira ngo byongere imbaraga z'umubiri. Nyuma yo guhindura genetike, adenovirus na lentivirus bikoreshwa nka vectors kugirango bitume habaho ubudahangarwa bw'umubiri binyuze mu kwigana virusi no kwerekana. Ingaruka yubudahangarwa irakora neza kandi iramba, ariko inzira yo kwitegura iragoye, dukeneye rero kugenzura byimazeyo ibinyabuzima. Mugihe mRNA ishobora gutumizwa mu ngirabuzimafatizo mu buryo butaziguye, kugira ngo selile zishobore kwerekana antigen kandi zishishikarize kubaho kw’ubudahangarwa bw'umubiri, iyi nzira iragoye, ibyo bikaba bidusaba kumenya niba mRNA ihagaze neza. Nubwo antijene ya recombinant itandukanye muguhinduka na antigene karemano, zirashobora kubyara umusaruro mwinshi mwinganda. Poroteyine ntoya cyangwa antigene ya polypeptide, irashobora gutegurwa muburyo bwa vitro synthesis. Imiterere yabyo irashobora kugenzurwa, ariko irashobora kugorana mugushushanya. Antigene ntoya ya molekile ahanini ni ibice bito bya molekile nka peptide na nucleotide, kubera ko bidakingiwe ubwabyo, bityo birashobora gukoreshwa gusa nka immunogène nyuma yo guhuza nabatwara macromolecular.
Ikibazo.
Ibyiza byimiterere ya VHH?

A.
Uburemere bwa molekuline ya nanoantibodies ni nto cyane, mubisanzwe hafi 12-15 kDa, kimwe cya cumi gusa cya antibody gakondo ya IgG. Imiterere ya kristu ni umupira wa rugby hafi 2,5nm ya diametre na 4.2nm z'uburebure. Imiterere yihariye ya molekuline ituma ingirabuzimafatizo zinjira neza, igice kigufi cyubuzima, hamwe n’impyiko nyinshi, binyuze mu nzitizi y’amaraso n'ubwonko. Nanoantibodies igizwe no kuzuzanya kugena uturere twumugongo. Uturere twuzuzanya twuzuye harimo CDR 1, CDR 2, na CDR 3. Uburebure bwa aside amine 3 kugeza 28 mukarere ka CDR 3 butuma ubushobozi bwo kubika isomero rya nanoantibody. Ugereranije na antibody gakondo ya CDR 3 ya acide 8 kugeza kuri 15 gusa, zimwe muntara za CDR 3 zifite CDR 3 ndende zadufashije kumenya epitopi yihishe hejuru ya antigen. Agace k'umugongo ka nanobodies karimo FR 1, FR 2, FR 3, na FR 4, hamwe na hydrophilique amino acide enye kuri FR 2, kandi iyi mutation yongerera amazi ya antibody. Umubano udasanzwe wa disulfide uri hagati ya CDR 1 na CDR 3 wongera imbaraga za antibodies munsi yumuvuduko mwinshi, ubushyuhe bwinshi, denaturant, nibindi bihe, bifasha kubyara no kubungabunga nanobodies, kandi binatanga uburyo bwuburyo bushya bwo kuyobora.

Niba ufite ikibazo, nyamuneka wumve nezatwandikireigihe icyo ari cyo cyose.

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Serivisi yihariye

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VHH Serivisi imwe ya Antibody Serivisi