Serivise Yerekana Isomero Serivisi yo Kubaka

Alpha Lifetech kabuhariwe mu kwerekana umusemburo werekana isomero ryubaka no kwerekana amasomero ya antibody, itanga ubushobozi bwo kubyara ubwoko butandukanye bwamasomero yimisemburo no kubisuzuma kugirango bihuze cyane na antibodi zihariye kubakiriya kwisi yose. Hamwe nitsinda ryabashakashatsi b'inzobere, ikoranabuhanga n'ibikoresho bigezweho, turashobora kubaka amasomero yimisemburo yibasira poroteyine zitandukanye ziterwa n'indwara.

Sisitemu yerekana inyuma

Kwerekana umusemburo ni tekinoroji ya eukaryotic yerekana uburyo bukoresha uburyo bwo guhinduranya poroteyine yo mu nda (GPI anchor) ya sisitemu yo kwerekana imisemburo kugira ngo ihagarike kandi yerekane antibodiyite zidasanzwe cyangwa ingirabuzimafatizo za poroteyine ku musemburo. Sisitemu yo kwerekana imisemburo ifite poroteyine yuzuye nyuma yo guhindura no guhindura uburyo bwo gusohora, ikaba ikwiriye cyane guhunika, guhindura no gutwara poroteyine zabantu. Kubwibyo, antibodies zakozwe na sisitemu yo kwerekana umusemburo yegereye antibodi zabantu kavukire haba mubikorwa ndetse no mumikorere, kandi bitanga amahirwe menshi yo kuvura indwara.

Sisitemu yo kwerekana umusemburo ituma habaho antibodies zihariye za poroteyine, peptide, molekile nto, hamwe na T-selile. Izi antibodies zifite ibyiza byo guhinduranya, kugereranya no kuvura neza. Ikoreshwa rya tekinoroji yerekana imisemburo iranagutse cyane, ntishobora gukoreshwa gusa mugusanga kwerekana antibody gusa, ahubwo no mugusuzuma isomero rya protein, isuzuma rya poroteyine ryerekanwe ubwihindurize, kubaka isomero rya antigen, gutondekanya antibody, gukura kw'inkingo, immuno-biocatalyse, na biosensor, n'ibindi, bifite ibyiringiro byinshi mubijyanye no gusuzuma, kuvura indwara n'ubushakashatsi mu buvuzi. Byongeye kandi, poroteyine zateguwe binyuze mumisemburo ya antigenic yerekana hafi yimiterere yabyo, bigatuma iba nziza kubisubizo bya serologiya nka ELISA. kwerekana umusemburo-Alpha Lifetech

Igishushanyo 1: Ihame ryerekana umusemburo

umusemburo werekana kubaka isomero Inzira

Amasomero yacu yimisemburo afite ubushobozi bunini kandi butandukanye, butanga urufatiro runini rwo gusuzuma neza antibody yihariye. Turashobora kubaka uburyo butandukanye bwububiko bwibitabo bwa antibody (IgG, scFv, VHH, amasomero ya antibody ya Fab), amasomero ya protein, amasomero ya peptide, amasomero ya cDNA, nibindi dukurikije ibyo ukeneye. Byongeye kandi, turashobora kandi guteza imbere amasomero ya antibody yibintu bitandukanye, harimo amasomero yumubiri, amasomero karemano, amasomero yubukorikori, amasomero yubukorikori, hamwe nububiko bwibitabo bya antibody. pYD nubusanzwe dukoresha umusemburo hejuru yerekana vector.

Turashoboye kubaka amasomero yimisemburo ifite ububiko bwibitabo bugera kuri 10 ^ 9, hamwe 100% yo kwinjiza no gutandukana. Mbere yo gutanga isomero ryimisemburo, turemeza neza kugirango tumenye ubudasa, ubunyangamugayo n'imikorere ya gen. Kugirango tumenye neza ko antibodies zerekanwe zifite isano iri hejuru kandi yihariye, dukoresha isuzuma ryigihe-nyabyo cyo gusuzuma isomero rya antibody. Fluorescence-ikora selile itondekanya (FACS) igereranya muburyo bwo guhindura imvugo n'imikorere ya antibody, ikoresha ibimenyetso bitandukanye kuri antibody na antigen kugirango hamenyekane imbaraga zihuza hamwe na antibody imvugo hejuru ya selile yimisemburo. Dukoresha FACS ihuza ibizamini kugirango igereranye ingano ya antibodies zifitanye isano ryinshi kandi rihitamo. Kugaragaza imikorere yumusemburo werekana isomero rya nanobody na FACS rirenze 95%. Mubisanzwe, gusuzuma umusemburo birimo ibice 3-5 byo gusuzuma no gukungahaza, bikadufasha kubona antibodiyite nyinshi za antibodiyite mugihe gito.

Sisitemu yo kwerekana umusemburo yahindutse igikoresho gikomeye mu bwihindurize bwa poroteyine. Alpha Lifetech itanga poroteyine imvugo, kweza, hamwe na serivisi isesengura isesengura rya vitro yo gutegura antibody yihariye. Dufite ubushobozi bwo gutegura poroteyine nini kandi tunoza neza ubuziranenge bwa poroteyine za recombinant. Dutanga serivisi imwe iva kuri synthesis ya gene kugeza recombinant protein imvugo no kwezwa. Twifashishije sisitemu yo kwerekana imisemburo igezweho, twateguye neza antibodies zifite picomolar nyinshi (pM), iyi ikaba ari intambwe ikomeye muri tekinoroji ya vitro recombinant.