Serivisi yo kubaka isomero ry'ibishushanyo mbonera by'umusemburo

Alpha Lifetech inzobere mu kubaka isomero ryerekana imisemburo no gusuzuma amasomero y’abarwanya indwara, itanga ubushobozi bwo gukora ubwoko butandukanye bw’amasomero y’umusemburo no kuyasuzuma kugira ngo hamenyekane niba afite imbaraga nyinshi kandi afite ubudahangarwa bwihariye ku bakiriya ku isi yose. Dufatanyije n’itsinda ry’abashakashatsi b’inzobere, ikoranabuhanga rigezweho n’ibikoresho, dushobora kubaka amasomero y’umusemburo twibanda ku bwoko butandukanye bwa poroteyine ziterwa n’indwara.

Inyuma ya sisitemu yo kwerekana umusemburo

Igaragaza ry'umusemburo ni ikoranabuhanga ryo kugaragaza poroteyine ya eukaryotike rikoresha uburyo bwo guhindura poroteyine mu ngirabuzima fatizo (GPI anchor) bw'uburyo bwo kwerekana ubuso bw'umusemburo kugira ngo buhagarike kandi bugaragaze antibodies zo hanze cyangwa genes za poroteyine ku buso bw'umusemburo. Uburyo bwo kwerekana ubuso bw'umusemburo bufite uburyo bwuzuye bwo guhindura no gusohora poroteyine nyuma yo guhindura no gusohora, bukwiriye cyane mu gupfunyika, guhindura no gutwara poroteyine zahinduwe abantu. Kubwibyo, antibodies zikorwa n'uburyo bwo kwerekana umusemburo zegereye antibodies gakondo z'abantu haba mu miterere no mu mikorere, kandi zitanga amahirwe menshi yo kuvura indwara.

Sisitemu yo kwerekana umusemburo ituma habaho uturemangingo twihariye twa poroteyine, peptides, molekile nto, na za receptor za T-cell. Izi poroteyine zifite ibyiza byo gukora ibintu bitandukanye, gupima no kuvura neza. Ikoreshwa ry'ikoranabuhanga ryo kwerekana umusemburo ni rinini cyane, rishobora gukoreshwa atari mu kuvumbura uturemangingo tw’uturemangingo tw’umusemburo gusa, ahubwo no mu gusuzuma isomero rya poroteyine, iterambere rya poroteyine, kubaka isomero rya antigen, gutondeka uturemangingo tw’uturemangingo twinshi, gukura kwa antibody, gukora urukingo, immuno-biocatalysis, na biosensors, nibindi, bifite amahirwe menshi mu bijyanye no gusuzuma indwara, kuvura indwara no gukora ubushakashatsi ku buvuzi. Byongeye kandi, poroteyine zakozwe binyuze mu kwerekana umusemburo wa antigen ziri hafi y’imiterere yazo karemano, bigatuma ziba nziza mu gusuzuma serological nka ELISA. kwerekana umusemburo-Alpha Lifetech

Igishushanyo cya 1: Ihame ryo kwerekana umusemburo

Uburyo bwo kubaka isomero ry'ibyerekanwa ry'umusemburo

Amasomero yacu y’umusemburo afite ubushobozi bwinshi kandi atandukanye cyane, ibyo bikaba bitanga ishingiro rikomeye ryo gusuzuma neza uruhererekane rw’uturemangingo twihariye tw’umubiri. Dushobora kubaka ubwoko butandukanye bw’amasomero y’uturemangingo tw’umubiri (IgG, scFv, VHH, Fab antibody libraries), amasomero ya poroteyine, amasomero ya peptide, amasomero ya cDNA, nibindi bitewe n’ibyo ukeneye. Byongeye kandi, dushobora no guteza imbere amasomero y’uturemangingo dufite imiterere itandukanye, harimo amasomero y’ubudahangarwa bw’umubiri, amasomero karemano, amasomero y’ubukorano, amasomero y’uturemangingo duto, n’amasomero y’uturemangingo tw’indwara. pYD ni vector yacu ikunze gukoreshwa cyane yo kwerekana ubuso bw’umusemburo.

Dushoboye kubaka amasomero y’umusemburo afite ubushobozi bwo kubika amakuru kugeza kuri 10^9, hamwe n’igipimo cyo gushyiramo amakuru 100% n’ubusumbane. Mbere yo gutanga isomero ry’umusemburo, twemeza neza ko imiterere y’uturemangingo tw’umubiri ishingiye ku budasa, ubusugire n’imikorere y’uturemangingo tw’umubiri. Kugira ngo twizere ko uturemangingo twasuzumwe dufite ubusumbane n’imiterere yihariye, dukoresha isuzuma ry’uburyo bwo gusuzuma amakuru y’uturemangingo mu buryo bwihuse. Gutondekanya uturemangingo tw’umubiri hakoreshejwe fluorescence (FACS) bipima impinduka mu mikorere y’uturemangingo n’imikorere yatwo, bikoresha ibimenyetso bitandukanye kuri antibody na antigen kugira ngo bimenye imbaraga zo gufatanya n’imikorere y’uturemangingo tw’umubiri ku buso bw’uturemangingo tw’umusemburo. Dukoresha ibizamini byo gufatanya na FACS kugira ngo dusuzume neza uturemangingo dufite ubusumbane n’uburyo bwo guhitamo. Ubuhanga bwo kwerekana amakuru y’isomero ryacu ry’umusemburo w’umubiri w’umubiri w’umusemburo na FACS burenze 95%. Ubusanzwe, gusuzuma umusemburo bikubiyemo isuzuma n’ubuhanga mu byiciro 3-5, bigatuma tubona uturemangingo tw’umubiri dufite ubusumbane mu gihe gito.

Sisitemu yo kwerekana umusemburo yabaye igikoresho gikomeye mu iterambere rya poroteyine riyobowe. Alpha Lifetech itanga serivisi zo gusesengura poroteyine, gutunganya no kugaragaza imiterere yayo mu gutegura antibody yihariye muri vitro. Dufite ubushobozi bwo gutegura poroteyine nini no kunoza neza ubuziranenge bwa poroteyine zisubiramo. Dutanga serivisi imwe kuva ku guhuza gene kugeza ku gukora poroteyine zisubiramo no gutunganya. Dukoresheje sisitemu yacu igezweho yo kwerekana umusemburo, twateguye neza antibody zifite imiterere ya picomolar (pM) ihanitse cyane, ikaba ari intambwe ikomeye mu ikoranabuhanga ryo gutegura antibody zisubiramo muri vitro.

Igishushanyo cya 2 Uburyo bwo kubaka isomero ry'ibishushanyo mbonera by'umusemburo

Ibyiza byo kwerekana umusemburo

Dufite ubushobozi bwo kubaka amasomero y’ibice by’ibice bifite ubushobozi bwo kubika amakuru kugeza kuri 10^9, hamwe n’uburyo bwo kuyashyiramo 100% n’uburyo butandukanye bwo kuyakoresha.