Single Cell Sequencing (SCS) technology, focusing on the Single Cell's genome, or group of transcription depth so as to get more information about genome, transcriptome and other multi-level Cell details, to accurately reveal the difference of Cell groups and Cell evolutionary context. Through this technology, we can understand the intricate heterogeneous mechanism behind the occurrence and development of diseases, and provide strong support for accurate diagnosis of diseases, prognosis assessment and the formulation and optimization of personalized treatment programs. Compared with traditional sequencing methods, they can only capture the average value of a large number of cell behaviors, ignoring a few key cells and their unique heterogeneous characteristics, thus limiting the comprehensiveness of information. Single cell sequencing, on the other hand, can be directly targeted at a single cell, not only accurately identifying the subtle differences between cells, but also effectively distinguishing cell types in a small number of cell samples, and even building a fine cell map. Early single-celled sequencing technologies because of high cost and limits its use scope, the list as the research and development of technology, many innovative single-celled sequencing technology arises at the historic moment, greatly reduce the cost of the technology threshold. At present, as a revolutionary tool in the field of biological research, single cell sequencing technology not only shines brightly in the basic scientific research exploring the mystery of life, but also shows great potential for transformation and practical application value in several key areas of clinical practice.

Fig. 1: Single-cell isolation and library preparation. (Reference source: Hwang, B., Lee, J.H. & Bang, D. Single-cell RNA sequencing technologies and bioinformatics pipelines. )