O le a le Antibody Engineering?

Faatasi ai ma le fesoasoani a le inisinia antibody, ua mafai ona suia le tele mole mole, pharmacokinetics, immunogenicity, sootaga vavalalata, faʻapitoa ma le faʻaogaina o gaioiga o antibodies. A maeʻa ona faʻapipiʻi faʻamaʻi, o le fusia faʻapitoa o faʻamaʻi e avea ma mea taua tele i suʻesuʻega ma togafitiga. E ala i le inisinia antibody, e mafai ona latou ausia manaʻoga o vailaʻau ma suʻesuʻega vave atinaʻe.

O le faʻamoemoega o le inisinia o le antibody o le faʻatulagaina ma le faʻatinoina o galuega faʻapitoa, mautu e le mafai ona ausia e antibodies masani, faʻavaeina le faavae mo le gaosiga o vailaʻau faʻamaʻi.

Alpha Lifetech, faʻatasi ai ma lona poto masani faʻatino i le inisinia antibody, e mafai ona tuʻuina atu auaunaga faʻapitoa mo monoclonal ma polyclonal antibody mo le tele o ituaiga, faʻapea foʻi ma le faʻaalia o le faletusi o le faletusi ma le suʻeina o auaunaga. E mafai e le Alpha Lifetech ona tuʻuina atu i tagata faʻatau ni faʻamaʻi faʻamaʻi lelei ma mea faʻapolotini recombinant, faʻapea foʻi ma auaunaga tutusa, e maua ai antibodies lelei, faʻapitoa, ma mautu. E ala i le faʻaaogaina o le antibody atoatoa, faʻapipiʻi porotini ma faiga faʻaaliga phage, matou te tuʻuina atu auaunaga e aofia ai le pito i luga ma lalo o le gaosiga o le antibody, e aofia ai auaunaga faʻapitoa e pei o le antibody humanization, antibody purification, antibody sequencing, ma antibody validation.

O le tulaga paionia o le inisinia antibody e fesoʻotaʻi ma tekinolosi e lua:

--Recombinant DNA tekinolosi

--Tekinolosi Hybridoma

O le televave o le atinaʻeina o le inisinia antibody e fesoʻotaʻi ma tekinolosi taua e tolu:

--Gene cloning technology and polymerase chain reaction

--Fa'amatalaga Porotini: O polotini toe tu'ufa'atasia e maua mai i faiga fa'aaliga e pei o le fefete, fa'ama'i fa'atusa o la'au, ma la'au.

--Komepiuta fesoasoani fausaga fausaga

O le ulua'i augatupulaga o fa'ama'i na fa'atagata soifua mo le gaosiga o chimeric antibodies, lea e feso'ota'i ai le itulagi fesuia'i o isumu monoclonal antibodies i le vaega faifai pea o mole mole IgG tagata. O le antigen binding region (CDR) o le fa'atupu lona lua o le kiore monoclonal antibody na fa'apipi'iina i le IgG tagata. Se'i vagana ai le itulagi CDR, o isi antibodies uma e toetoe lava o tagata antibodies, ma sa faia taumafaiga e aloese mai le fa'aosoosoina o tagata anti mouse antibody (HAMA) tali pe a fa'aogaina fa'ama'i clone mole mo togafitiga tagata.

 

Ina ia fausia se faletusi faʻaaliga faʻaalia, o le laasaga muamua o le mauaina lea o kenera e faʻapipiʻi ai faʻamaʻi, lea e mafai ona vavae ese mai sela B o manu faʻamaʻi (fausiaina o le faletusi), aveese saʻo mai manu e leʻi faia tui puipui (fausia faletusi natura), poʻo le faʻapipiʻiina i vitro ma vaega o kenera antibody (fausia faletusi gaosi). Ona, o kenera e faʻateleina e PCR, faʻaofi i totonu o plasmids, ma faʻaalia i faiga faʻafeiloaʻi talafeagai (faʻafefete faʻafefete (e masani lava Pichia pastoris), faʻaaliga prokaryotic (masani E. coli), faʻamatalaga sela mamame, faʻaaliga sela o laʻau, ma le faʻaaliga o iniseti ua aʻafia i siama faʻapipiʻi tootoo). O le mea e sili ona taatele o le E. coli expression system, lea e tuʻufaʻatasia ai se faʻasologa faʻasologa o le antibody faʻapitoa i luga o le phage ma faʻapipiʻi se tasi o palatini atigi phage (pIII poʻo le pVIII). Le fusuaga o le kenera o, Ma fa'aalia i luga o le mata o bacteriophages. O le 'autu o lenei tekinolosi o le fausia lea o se faletusi fa'aaliga phage, lea e sili atu nai lo faletusi fa'anatura ona e mafai ona i ai se fa'amaufa'ailoga fa'apitoa. Mulimuli ane, o antibodies ma antigen specificity e suʻesuʻeina e ala i se faiga filifiliga faʻale-aganuʻu, faʻamautu faʻamaʻi faʻamaʻi, phages e leʻi fusifusia e faʻasolosolo ona fufulu ese, ma phages fusifusia e fufulu ese mo le faʻatamaoaigaina atili. A maeʻa le tolu pe sili atu taʻamilosaga o le toe fai, e faʻamavaeina faʻamaʻi faʻapitoa ma maualuga faʻatasi.

Ata 3: Fausiaina ma le Su'esu'eina o le Faletusi o Antibody

Recombinant DNA technology e mafai ona fa'aogaina e fa'atupu ai vaega o le antibody. Fab antibodies e mafai muamua ona fa'asusuina e le gastric protease e maua ai (Fab ') 2 fasi vaega, ona fa'aliloina lea e papain e fa'atupuina ai vaega Fab ta'itasi. O le Fv fragment e aofia ai le VH ma le VL, lea e le lelei le mautu ona o le leai o ni faʻamaufaʻailoga disulfide. O le mea lea, VH ma VL e fesoʻotaʻi faʻatasi e ala i se peptide puʻupuʻu o 15-20 amino acids e faʻatupu ai se vaega faʻasolosolo e tasi (scFv) antibody ma se mamafa mole e tusa ma le 25KDa.

O le suʻesuʻega o le fausaga o le antibody i Camelidae (Camel, LIama, ma le Alpaca) ua faʻamaonia ai o antibodies e naʻo filifili mamafa ae leai ni filifili mama, o lea ua taʻua ai i latou o antibodies mamafa (hcAb). Ole vaega fesuia'i o fa'ama'i filifili mamafa e ta'ua o fa'ama'i fa'anofo tasi po'o nanobodies po'o VHH, fa'atasi ai ma le tele o le 12-15 kDa. I le avea ai ma monomers, latou te leai ni faʻamaufaʻailoga disulfide ma e matua mautu, ma se maualuga maualuga mo antigens.

Ata 5: Antibody filifili mamafa ma le VHH/ Nanobody

Ole fa'aaliga saoloto ole cell e fa'aogaina ai le fa'aaliga ole DNA fa'anatura po'o le gaosia ole DNA e ausia ai le fa'atupuina o le porotini in vitro, e masani ona fa'aogaina le E. coli expression system. E vave maua mai polotini ma aloese ai mai le metabolic ma le cytotoxic avega i luga o sela pe a gaosia le tele o polotini recombinant i vivo. E mafai fo'i ona maua mai polotini e faigata ona fa'apipi'iina, e pei o mea e faigata ona fa'aleleia pe a mae'a fa'aliliu pe fa'apipi'i polotini membrane.