Puratifomu yeAptamer Synthesis
Zvichienderana nechinangwa, ma aptamers e nucleic acid anosanganisira ma aptamers eDNA, ma aptamers eRNA, ma aptamers eprotein, uye ma aptamers e membrane ye cell.
TAURA NESU01
Puratifomu yeAptamer Synthesis
Nhanganyaya yeNucleic acid aptamers
Nucleic acid aptamers, inozivikanwawo se aptamers, complexes, uye chemical antibodies, inowanikwa ne in vitro selex screening ne ligand exponential enrichment system evolution (SELEX). I single-stranded deoxyribonucleic acid (ssDNA) kana ribonucleic acid (RNA) inobatanidza zvakananga uye zvinobudirira kune molecule yakanangwa, uye inowanzova nemabhesi makumi maviri kusvika makumi masere.
Ma aptamer eNucleic acid anowanikwa kuburikidza nekuongorora in vitro akasimba pachimiro, ari nyore kugadzira uye ari nyore kugadzirisa, uye akanakisa kupfuura ma antibodies anoshandiswa zvakanyanya mukugadzirwa uye kushandiswa; pamusoro pezvo, ma aptamer enucleic acid anogona kubatana zvakananga kune zvinangwa zvawo kuburikidza nemasimba akasiyana-siyana, kazhinji, anosangana nemamorekuru madiki zvichibva pane zvakasiyana-siyana (semuenzaniso, hairpins, concave-convex, pseudo-twisted, nezvimwewo) kuburikidza nehydrogen bonding, electrostatic forces, hydrophobic interactions, nezvimwewo mamorekuru. Zvichienderana nechinangwa, ma aptamer enucleic acid anosanganisira ma aptamer eDNA, ma aptamer eRNA, ma protein aptamers, uye ma aptamer e membrane cell.
Chikuva cheAlpha Lifetech chekugadzira aptamer, icho chinonyanya kusanganisira sevhisi yeSELEX aptamer library synthesis service uye aptamer (DNA, RNA kana XNA) yekugadzira, pamwe nekuongorora aptamer, kugadzirisa aptamer, uye masevhisi ekuongorora kuzivikanwa kweaptamer.
Maitiro ehunyanzvi ekugadzira Aptamer
Nheyo Yekutanga uye Maitiro Etekinoroji
Maitiro ekugadzira nucleic acid aptamer synthesis tekinoroji ndeyekuti raibhurari yemaoligonucleotides ane tambo imwe chete inogadzirwa nemakemikari mu vitro, yakasanganiswa nechinhu chinotarirwa, uye complex yechinhu chinotarirwa ne nucleic acid iripo mumusanganiswa, nucleic acid isingabatanidzi kune chinhu chinotarirwa inosukwa, uye mamolekyu e nucleic acid anosungana kune chinhu chinotarirwa anoparadzaniswa, uye mamolekyu e nucleic acid anoshandiswa sematemplate ekuita PCR amplification yechikamu chinotevera chekuongorora. Kuburikidza nekudzokorora selex screening uye amplification, mamwe mamolekyu eDNA kana RNA asingabatanidzi kune chinhu chinotarirwa kana ane low kana mwero affinity kune chinhu chinotarirwa achasukwa, uye maaptamers, kureva, DNA kana RNA ine high affinity kune chinhu chinotarirwa, achaparadzaniswa neraibhurari yakakura kwazvo, uye kuchena kwawo kuchawedzera nemaitiro eSELEX, uye pakupedzisira achatora chikamu chikuru cheraibhurari.
Pfungwa Dzinokosha dzeMaitiro eUnyanzvi
Maitiro ekuongorora maaptamer enucleic acid e selex anotanga nekuunganidzwa kwemaraibhurari e random oligonucleotide akagadzirwa nemakemikari. Maraibhurari e random oligonucleotide ane simba guru reraibhurari. Kusiyana-siyana kwakanyanya ndiyo mukana wayo mukuru. Panyaya yedzidziso, kana kutevedzana kwakasarudzika mu oligonucleotide kuine mabhesi e n, simba reraibhurari i4n. Kana tikafunga nezveraibhurari yekuchinja kwe artificial, zvichawedzera kusiyana kwezvikamu zvisina kurongwa. Parizvino, kureba kwezvikamu zvisina kurongwa zve oligonucleotides zvinowanzo shandiswa mukuyedza kweSELEX kunowanzova mabhesi makumi matatu, uye simba reraibhurari rinogona kusvika pa10^18. Kugona kweraibhurari kwakakosha pakuongorora ma ligands e high-affinity selex. Zvisinei, sezvo huwandu hwemaraibhurari e synthetic hwakajeka, kutevedzana kwakareba zvakanyanya kunoderedza kuwanda kwe oligonucleotide yega yega mumaraibhurari, izvo zvichaderedza mukana wekuongorora selex kune aptamer chaiyo. Kazhinji, kugona kweraibhurari kwe10^13~10^15 muchikamu chekutanga chekuongorora selex kunogona kusangana nezvinodiwa zvinoshanda.
Mufananidzo 1 Maitiro ehunyanzvi eSELEX
zvakanakira zveNucleic acid aptamer
| Zvakanakira | Zvidimbu |
|---|---|
| Zvakananga | Kudzivisa maitiro echinyakare ekuyedza mhuka uye kusarudza zvakananga kubva mumaraibhurari e in vitro; kuongorora selex aptamers emamorekuru anotarisirwa asina immune system, asina immune system yakawanda kana kuti ane chepfu. |
| Mhando dzakasiyana dzemashandisirwo | Mamorekuru anotarisirwa anogona kuva madhayi eorganic, maamino acid, mapuroteni, mishonga inorwisa mabhakitiriya, mapeptide, mavhitamini, mishonga, kunyange masero, utachiona, mavhairasi, matishu, nezvimwewo. |
| Kubatana Kwakasimba | MaAptamers anoratidza kunyatsojeka uye hukama hwakanaka pakuongorora mamorekuru ari mu vitro. |
| Kugadzikana | MaAptamer madiki, ari nyore kuwana, anogona kudzokororwa mukugadzirwa, uye kugadzikana kwawo kunogona kuwedzerwa nekugadzirwa kwemakemikari uye kugadziriswa. MaAptamer aya anogara kwenguva refu mukugadzirwa kwemakemikari, anogara kwenguva refu uye anogona kutakurwa patembiricha yemumba. |
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Mishonga yekiriniki: kurapwa kwakanangana negomarara, nzira dzekuongorora kenza
Mishumo yechikwereti yeGladtrust yakagadzirwa zvinobudirira zvichibva padhatabhesi reruzivo rwechikwereti chebhizinesi repamhepo - CreditVision.
Dhatabhesi iyi inounganidza uye inovandudza ruzivo rwemashoko anopfuura 200,000 panguva yakakodzera. Maitiro ekuvandudza nekutarisira ruzivo anogona kuvimbisa kururama kweruzivo.
Dhatabhesi iyi inounganidza uye inovandudza ruzivo rwemashoko anopfuura 200,000 panguva yakakodzera. Maitiro ekuvandudza nekutarisira ruzivo anogona kuvimbisa kururama kweruzivo.
02/
Nzvimbo yekuongorora: kuona zvinhu zvinosvibisa zvisikwa, kuona mishonga midiki yemamorekuru, kuona hutachiona hunobva muchikafu
Mundima iyi, tekinoroji yekugadzira nucleic acid aptamer ichashandiswa pamwe chete necolloidal gold immunochromatography, umo colloidal gold ichatanga yagadzirwa uye mhando yayo ichaonekwa, uye yozobatanidzwa nenucleic acid aptamer, iyo inoonekwa uye inopfapfaidzwa padanda rekusunga, uye test strips ichagadzirwa uchishandisa polyclonal antibody ichaonekwa semutsetse wekuona, uye kutevedzana kwenucleic acid aptamer semutsetse wekutonga mhando, kuitira kuti pave nekuongorora kuvapo kwechinhu chakanangana nacho zvichibva pamhedzisiro yekuedza kwakaipa uye kwakanaka.
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Munda wezvinhu zvipenyu: kukura kwema biosensors
Nucleic acid aptamer sensors dzinoshandisa aptamer sechinhu chekuziva chinhu chaunoda uye kushandura mhedzisiro yekuziva kuita chiratidzo chinogona kuyerwa. Kushandurwa kwechiratidzo kunogona kuva electrochemical, optical, temperature, piezoelectric, magnetic uye micromechanical kana musanganiswa wematekiniki aya. Zvichienzaniswa nenzira dziripo dzekuona, ma sensors akavakirwa panucleic acid aptamer anowanzo kuve nyore kushandisa, chaiwo, anopindura uye anonzwa, uye mabhenefiti aya anoita kuti zvive nyore kushandisa mumasampuli chaiwo uye ane mikana yakanaka yekuvandudzwa.
Sevhisi yeSELEX Aptamer Synthesis
Basa reAlpha Lifetech rekugadzira aptamer reSELEX rinosanganisira kuvaka raibhurari yeSELEX aptamer, kugadzirisa SELEX, nezvimwewo. Alpha Lifetech ine chinangwa chekuwana attamers dzeSELEX dzakabatana zvakanyanya uye dzakanyatsojeka kune vatengi kuburikidza nemaitiro ekuongorora in vitro.
VERENGA ZVIMWE
Basa reKugadzira Aptamer yeNucleic Acid
Basa rekugadzira nucleic acid aptamer reAlpha Lifetech rinonyanya kushandiswa netekinoroji yeSELEX yekuongorora SELEX aptamer, kusanganisira kugadzira DNA aptamers, RNA aptamers uye XNA aptamers, zvinosanganisira dhizaini yeraibhurari, kugadzirwa kweoligonucleotide, kuvakwa kweraibhurari uye kusimbiswa kwayo.
VERENGA ZVIMWEKana muine mibvunzo, ndapota inzwa wakasununguka kutibata chero nguva.
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2018-07-16 
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