Puratifomu Yekuvandudza MaAntibody Ane Bispecific

Alpha Lifetech inogona kupa vatengi zvigadzirwa zvemaantibody zvine hungwaru uye masevhisi ekugadzira mapuroteni akasanganiswa. Tinogona kugadzira maantibody ane simba rakawanda, akajeka, uye akasimba. Alpha Lifetech ine michina yakasiyana-siyana yekuchenesa maantibody, iyo inogona kupa masevhisi ekuchenesa maantibody kubva kunzvimbo dzakasiyana-siyana dzakadai setsuro, gwai, huku, uye mbeva monoclonal antibodies, pamwe neProtein A/G affinity cleansing services uye antibody separation and cleansing services. Zvichibva pakuvaka system yakazara ye platform ye antibody discovery platform, protein platform, nezvimwewo, tinofukidza masevhisi ekumusoro nekudzika ekugadzira maantibody, uye tinogona kupa masevhisi ehunyanzvi kubva pakugadzirira maantibody, bispecific antibody cleansing and antibody separation and cleansing, antibody sequencing, antibody validation, nezvimwewo, zvinogona kushandiswa pakurapa maantibody maviri.

Ma antibodies eMonoclonal anoreva ma immunoglobulins anogadzirwa nesero rimwe chete reB rine hunhu hwakanyanya hwema antigen kana epitopes. Kugadzirwa kwema antibodies eMonoclonal kwakagadzirwa pakutanga uchishandisa tekinoroji ye hybridoma kugadzira ma antibodies embeva. Kunyanya, nekubatanidza masero espleen kubva kumakonzo akapihwa majekiseni nemasero e myeloma kubva kuvanhu kana mbeva, masero e hybridoma anoumbwa, ayo anoburitsa ma antibodies chaiwo. Ma antibodies emonoclonal anogadzirwa embeva anonyanya kushandiswa mukutsvaga mhuka uye kuongororwa kwezvirwere mushure mekucheneswa. Zvisinei, muviri wemunhu unozogadzira mhinduro yekudzivirira muviri kumapuroteni embeva ekunze, saka pane zvipingamupinyi zvakakura mukushandiswa kwekiriniki kwekugadzirwa kwema antibodies emonoclonal embeva. Gare gare, vanhu vakashandisa "humanization" kugadzirira ma antibodies emonoclonal kubva kumakonzo, uye vakashandisa tekinoroji yekugadzira majini kugadzirisa ma antibodies embeva kuti ave nenzvimbo inogara iripo ye immunoglobulin yemunhu kuderedza immunogenicity yavo. Rudzi urwu rwe antibody runozivikanwawo se humanized monoclonal antibody. Pamusoro pezvo, kushandisa masero evanhu kugadzira ma antibodies emonoclonal kunonzi kugadzirira kwe whole human monoclonal antibody. Ma antibodies emonoclonal anoshandiswa zvakanyanya mukushandiswa kwe biomedical uye kliniki. Mukutsvaga kwezvekurapa, ma antibodies e monoclonal anogona kushandiswa sema immunomodulators, ma antibodies e monoclonal anorapa anogona kushandiswa pakurapa cancer chemotherapy nehutachiona hwehutachiona, uye kugadzirwa kwe monoclonal antibody conjugates kunonyanya kushandiswa pakurapa cancer.

Nhanganyaya yeBispecific Antibody

Muna 1960, pfungwa yema antibodies e-bispecific yakataurwa. Ma antibodies e-bispecific, anozivikanwawo se bispecific monoclonal antibodies, ma antibodies akagadzirwa nemaoko achishandisa tekinoroji yekugadzira majini. Sema antibody ekugadzira, ma antibodies e-bispecific anowanzo kuve echikamu cheIgG uye ane chidimbu chinosunga antigen chinotarisa CD3 subunit. Ma antibodies e-bispecific ane nzvimbo mbiri dzakatarwa dzekubatanidza ma antigen dzinogona kusunga nekuziva ma antigen maviri akasiyana panguva imwe chete, kana kuti ma epitopes maviri akasiyana eantigen. Zvichienzaniswa nema monoclonal antibodies, ma antibodies e-bispecific ane imwe nzvimbo yekusunga ma antigen chaiyo, nokudaro aine hunyanzvi hwakasimba uye kugona kutarisa, izvo zvinogona kunyatsotarisa masero egomarara uye kuderedza chepfu kubva kune chinangwa. Ma antibodies e-bispecific anogona kuita mabasa akawanda ebhayoloji panguva imwe chete, akadai sekuunganidza masero ekudzivirira muviri, kuvhara nzira dzekutumira zviratidzo, uye kuuraya zvakananga masero egomarara. Ma antibodies ekutanga e-bispecific ainyanya kugadzirwa kuburikidza nekubatana kwemakemikari kana kubatanidza masero, asi nzira iyi ingangodaro yakafambira mberi zvishoma nezvishoma nekuda kwekubatana kwakasarudzika uye kuoma kwekuparadzanisa musanganiswa wechinangwa. Nekuvandudzwa kuri kuramba kuchiita tekinoroji yekugadzira majini, mapuratifomu mazhinji matsva etekinoroji akagadzirwa, akadai semaknots in holes (KIH), CrossMab, DVD Ig, nezvimwewo. Mapuratifomu aya anogadzirisa matambudziko akadai sekusiyana kweheavy chain nelight chain, uye anovandudza kufanana uye goho rema antibodies maviri chaiwo.

Mufananidzo 1 Ongororo yakapfava yemaitiro anokurudzirwa ekuita kwemasoja emuviri (bsAbs) mumiedzo yekiriniki yekenza.(Mufananidzo wakabva: Ongororo yemasoja emuviri anokonzera chirwere chekenza uye maantibody akagadzirwa muzvirwere zvekenza - ScienceDirect)

Tekinoroji yekugadzira maAntibodies maviri

Nzira huru dzekugadzira maantibody maviri dzinosanganisira makemikari ekubatanidza, mana source hybridoma, uye gadziriro yemaantibody ekugadzira majini. Pakati padzo, nzira yekubatanidza makemikari inobatanidza zvidimbu zviviri zveIgG kana zviviri zveF (ab ') 2 antibody kuita maantibody maviri ega ega uchishandisa maantibody ekubatanidza makemikari akadai sephthalimide nedithioacylbenzoic acid. Nzira iyi iri nyore uye iri nyore kushandisa, asi inogona kukuvadza nzvimbo yekubatanidza maantigen, kuderedza mashandiro emaantibody, uye coupling agent pachayo inewo mwero wakati wecarcinogenicity. Nzira ina dzema source hybridoma dzakavakirwa pakubatanidzwa kwemasero emuviri kubva kumitsara miviri yakasiyana yema hybridoma cell kuratidza IgG yembeva inoenderana. Kuburikidza ne genetic engineering technology, ma antibodies anogona kugadziriswa majini kuti agadzire ma antibodies maviri ega ega. Ma antibodies maviri akasiyana emonoclonal akagadzirwa, uye zvidimbu zveFab kana heavy chain uye light chain variable regions ema antibodies maviri akapatsanurwa zvakasiyana. Kuburikidza ne cross-linking reaction kana chain recombination technology, zvidimbu zviviri izvi zvakabatanidzwa kuti zvigadzire bispecific antibody. Kunyangwe kuoma kwe tekinoroji yekugadzira majini kwakanyanya, parizvino ndiyo nzira inonyanya kushandiswa yekugadzira maantibody maviri kuti igadzirise chimiro uye mashandiro emaantibody. Pakugadzira maantibody maviri, musimboti we antibody cross reactivity unogona kushandiswa, asi antibody cross reactivity inogona kukonzera kusawirirana, saka ichanyatsofungwa nezvayo mukushandiswa kwemaitiro e antibody maviri, senge bispecific antibody therapy.

Kucheneswa kweMaAntibodies eBispecific

Kucheneswa kwemasoja emuviri eBispecific inzira yekuparadzanisa nekuchenesa masoja emuviri e-high-purity target antibodies. Nzira mbiri, centrifugation uye deep filtration, zvinoshandiswa kubvisa tsvina inonyungudika. Soja yemuviri yebispecific antibody inotanga yabatwa ne affinity chromatography. Kune masoja emuviri eIgG akafanana ne bispecific antibodies, protein A affinity chromatography inoshandiswa, nepo kune masoja emuviri eIgG akafanana ne bispecific antibodies, light chain based affinity chromatography inogona kushandiswa. Mushure mezvo, soja yemuviri inoiswa pasi pe pH yakaderera kwenguva yakati, zvichiita kuti chimiro cheprotein pamusoro pevhairasi chikanganiswe, nokudaro ichirasikirwa nekukwanisa kutapurira masero. Intermediate deep filtration inoitwa kuti ibvise tsvina senge mapuroteni emasero emuviri (HCP). Kuchena kwema antibodies kunovandudzwa kuburikidza nenzira dzakadai se ion exchange chromatography, uye chero mavhairasi akasara anobviswa kuburikidza ne nanofiltration kana ultrafiltration techniques. Pakupedzisira, sample inoiswa pamwe chete uye inotsiviwa ne formulation buffer yakakodzera.

Basa rekugadzira MaAntibodies eBispecific

Matanho	Zviri Mukati meSevhisi	Nguva yekutevera nguva
Kugadzirwa kweMajini	Kugadzirwa kwezvirongwa zvitatu zvakasiyana zvekugadzira, kugadzirwa kutsva kwe recombinant antibody DNA plasmids	Mavhiki maviri kusvika matatu
Bvunzo Diki	Kugadzirwa kwemapuroteni e-antibody maviri → Kuratidzwa kudiki mumasero emhuka dzinoyamwisa → Kusimbiswa neSDS-PAGE → Kuongororwa kwekubatanidza nemaantigen eprotein anowanikwazve neELISA → Kuenzaniswa kwema clones nema antibodies evabereki	Mavhiki mashanu kusvika matanhatu
Ziva	Kuratidzwa kwema antibodies maviri ekutanga akazara-refu bispecific → Ongororo yekubatanidza neELISA → Ongororo yeELISA yema antibodies anozivikanwa nema anti antibodies embeva	Vhiki imwe chete
Kugadzirwa kweAntibody	Kugadzirwa kwema antibodies akawanda	Mavhiki matatu kusvika mana

Zvakanakira Zvedu

Tinofukidza mabasa ekugadzira maantibody ekumusoro neapasi, uye tinogona kupa mabasa ehunyanzvi kubva pakugadzira maantibody, kucheneswa kwemaantibody maviri uye kupatsanurwa kwemaantibody maviri, kutevedzana kwemaantibody, kusimbiswa kwemaantibody, nezvimwewo, izvo zvinogona kushandiswa pakurapa maantibody maviri.

Kuratidza Kukuru

Kuwanda kwemapuroteni mumasero eCHO.
Kuchengetedza Kwakagadzikana

> mavhiki maviri mu serum pa37°C.
Hapana Dambudziko reKunyunguduka

>30 mg/ml
Masero Akagadzikana

Mitsetse yakawanda yemasero yakagadzikana ine maantigen egomarara yakagadzirwa kuti isimbise ma antibodies akawanda e-bispecific