Basa rekuwana maAntibody reFab
Chikwata cheAlpha Lifetech chekutsvaga ma antibodies chinodada kuzivisa kuti tinogona kugadzira raibhurari yeFab yakagadzirwa ine ma clones akazvimiririra anopfuura 10^11.
TAURA NESUBasa rekuwana maAntibody reFab
Alpha Lifetech Inc.inokwanisa kupa mhando dzakasiyana kana ma isotype akasiyana emaraibhurari eFab antibody emuno kana akagadzirwa zvichibva papuratifomu yedu yehunyanzvi hwekuratidza ma phage. Kuvaka raibhurari yemhando yepamusoro ye antibody ine raibhurari yakakura uye yakasiyana-siyana kwave kwakakosha kuti ma antibodies abudirire, zvichakonzera kukurumidza kuwanikwa kwemishonga ye antibody. Iye zvino.Alpha Lifetech'sChikwata cheR&D chekutsvaga maantibody chinodada kuzivisa kuti tinogona kugadzira raibhurari yeFab yakagadzirwa ine ma clones akazvimiririra anopfuura 10^11.
Chii chinonzi Fab Antibody?
Molekyuru yeIgG inogona kukamurwa kuita ma subunits maviri anoshanda: (1) chidimbu chinobatika (Fc), chinoumba muswe weantibody uye chinoshanda nema cell surface receptors kuti chiite kuti immune response ishande, uye (2) chidimbu-antigen binding (Fab), chinobata kuziva maantigen. Dunhu reFc rine ma peya maviri ema constant domains (CH2 neCH3) kubva kumacheni maviri anorema akabatana, nepo Fab region ye antibody ine variable domain inoteverwa ne constant domain kubva kumacheni anorema (VH neCH, zvichiteerana), ayo akabatana ne variable uye constant domain kubva kumacheni anorema (VL neCL, zvichiteerana).
Tekinoroji yeFab Antibody Development-Phage Display
Maraibhurari eFab, umo majini enzvimbo inoshanduka-shanduka (LC) uye majini enzvimbo inoshanduka-shanduka (HC) anounganidzwa kuita phagemid vector uye ozoratidzwa pamusoro pe filamentous phage particle, akashandiswa zvakanyanya pakupatsanura ma antibodies (Abs) ane hunhu hwe haptens, ma antigen ekune dzimwe nyika (Ags), uye ma self Ags.
Alpha Lifetech inogona kupa basa rekuvaka nekuongororwa kweraibhurari yeFab uye basa rekuvaka nekuongororwa kweraibhurari yeFab yakagadzirwa.
Sei Uchifanira Kusarudza Isu?
● Kugadziriswa kweVector
Masayendisiti edu akagadzirisawo sisitimu yevector ine zvinhu zvakasiyana-siyana, zvakaita sekutungamira kwakasiyana kwecheni dzakareruka nedzinorema, codon yekumisa inobvumira kufamba nyore nyore pakati pemhando dzakakodzera dzemugadziri we phage kana kutaura kwe soluble Fab, Myc tag yekuongorora nekucheneswa kweprotein, uye subtilisin cleavage site inobatsira kudzoreredza bound phage panguva yekuongorora raibhurari.
● Nzira yeKugadzira Makopi
Kuti vagadzire raibhurari yakakura yemasoja ekudzivirira chirwere, Alpha Lifetech Inc. inoshandisa nzira inoshanda yekuunganidza ma "cloning" pakuvaka maraibhurari emasoja ekudzivirira chirwere zvichibva pakupatsanurwa kwezvidimbu zve "restriction fragments" kubva kuma "plasmid vectors" pachinzvimbo chezvigadzirwa zvePCR.
● Basa reKumira Rimwe Chete
Nekunzwisisa kwakadzama kwetekinoroji yeraibhurari yekuratidza maantibody phage, tinogona kugadzirisa chirongwa chenyu kuti chienderane nekuvakwa kamwe chete uye kuongororwa kwemaraibhurari eantibody, kubatsira vatengi nematambudziko ari kuuya mukuvakwa nekuongororwa kwemaraibhurari.
Sevhisi ine chekuita neFab AntibodyBASA RINE CHOKUITA
Basa rekuvaka raibhurari yeFab Antibody
Alpha Lifetech ine sisitimu yakakura yekuvaka raibhurari yeFab antibody, iyo inogona kuvaka maraibhurari eFab antibody pamhuka dzevanhu nemhuka dzakasiyana-siyana, kusanganisira maraibhurari ekudzivirira zvirwere, maraibhurari emuno, maraibhurari ekugadzira zvinhu zvishoma, maraibhurari ekugadzira nezvimwewo, izvo zvinogona kusangana nezvinodiwa nevatengi zvakasiyana.
Ongorora
Basa rekuongorora raibhurari yeFab Antibody
Alpha Lifetech inogona kuongorora maraibhurari akasiyana-siyana e phage zvichibva paM13, T4, T7 ne λ kubva kumarudzi evanhu, mbeva, tsuro, huku, makwai nengamera zvichienderana nezvinodiwa nevatengi vedu.
Ongorora
Basa reFab Antibody Expression
Alpha Lifetech inogona kugadzira ma antibodies eFab akawanda nekushandisa nzira yekukura, ma vector ekutaura, mamiriro e transfection, nezvimwewo kuti zvishandiswe mukutsvaga mishonga, biologics nedzimwe nzvimbo.
Ongorora
Basa rekuchenesa maAntibody reFab
Alpha Lifetech inochenesa ma antibodies eFab kubva kumasero ekuchengetedza masero uye serum ne affinity chromatography, uye kuchena kwema antibodies akacheneswa kunogona kusvika kupfuura 95%.
Ongorora
Basa reFab Antibody Biotinylation
Basa reFab antibody biotinylation rinopihwa neAlpha Lifetech inyanzvi yekunyora maantibody inoshanda zvikuru iyo inowedzera kunzwisisika kwekuona uye kugadzikana kwema antibodies nekubatanidza mamorekuru ebiotin kune ma antibodies eFab.
Ongorora
Basa rekutsvaga mashandiro eFab Antibody
Ongorora zvakakwana mashandiro uye kugadzikana kwema antibodies eFab, uchipa data rakadzama rehunhu. WB, IHC, ICC, IF, ELISA, IP, ChIP, peptide array, flow cytometric analysis (FC)
Ongorora
Basa reFab reKuita Munhu Anodzivirira Kurwara
Zvichibva pane zvakatevedzana zvemaantibody ekutanga zvakapihwa nemutengi, kuenzanisa kutevedzana uye kutevedzera kwemakombiyuta zvinoitwa kugadzira hurongwa hwekuita kuti munhu ave munhu.
Ongorora
Basa reFab Antibody Labeling
Alpha Lifetech inowana kubatana kwakasimba kwema antibodies ku biotin, HRP, uye isotopes kuburikidza nemaitiro ekunyora mavara, izvo zvinovandudza zvakanyanya kunzwisisika uye kujeka kwekuona.
Ongorora
Basa rekuvaka raibhurari reNaïve Fab
Mhando dzakasiyana-siyana dzemaraibhurari eFab antibody: maraibhurari ekudzivirira muviri, maraibhurari emuno, maraibhurari ekugadzira, nezvimwewo. Tinotaura nezvemabasa ekugadzira maantibody eFab kumusoro nekuzasi.
Ongorora
Basa rekuongorora raibhurari yeNaïve Fab
Kuburikidza nematanho maviri kusvika matatu ekudzivirira chirwere, ma antibodies eFab anobatana nema antigen akasiyana-siyana ane hukama hwakakura anogona kuparadzaniswa mukati memazuva gumi nemaviri pasina chikonzero chekubaya mhuka, izvo zvinopfupisa nguva yekugadzira ma antibodies eFab. Maraibhurari asina ruzivo ane huwandu hwakawanda hwema antibodies.
Ongorora
Basa rekuvaka raibhurari yeFab yakagadzirwa
Mhando dzakasiyana-siyana dzemaraibhurari eFab antibody: maraibhurari ekudzivirira muviri, maraibhurari emuno, maraibhurari ekugadzira, nezvimwewo.Alpha Lifetech ine chikuva chakazara chekuratidza M13/T7 phage kuti ive nechokwadi chekuvakwa nekugadzwa kwemaraibhurari emasoja ekudzivirira chirwere.
Ongorora
Basa rekuongorora raibhurari yeFab yakagadzirwa
Kuburikidza nematanho maviri kusvika matatu ekudzivirira, ma antibodies eFab anosunga kune akasiyana maantigen ane hukama hwakakura. Alpha Lifetech inogona zvakare kupa vatengi sarudzo yePhage display: nanobody phage display, scFv phage display, uye Fab phage display.
Ongorora
Sevhisi yeFab PEGylation
Alpha Lifetech inogonawo kupa mabasa akagadzirirwa iwe seFab antibody production, antibody labeling, antibody crosslinking, uye high throughput antibody screening kuti ienderane nezvaunoda.
OngororaKana muine mibvunzo, ndapota inzwa wakasununguka kutibata chero nguva.
2018-07-16 
