Puratifomu yeTekinoroji yeHybridoma
Alpha Lifetech inopa vatengi zvigadzirwa zvemhando yepamusoro zveantibody uye recombinant antibody.
Kumbira Mutengo01
Puratifomu yeTekinoroji yeHybridoma
Alpha Lifetech ine ruzivo rwakakura mukugadzira ma antibodies. Mumakore apfuura, Alpha Lifetech yakagadzira puratifomu yakazara yekugadzira ma antibodies (kusanganisira kugadzira ma antibodies e monoclonal, kugadzira ma antibodies e polyclonal, kugadzira ma antibodies e monoclonal recombinant, uye mamwe mabasa ekugadzirira nekugadzira ma antibodies).
Alpha Lifetech inopa vatengi zvigadzirwa zve antibody uye recombinant antibody zvemhando yepamusoro, uye ma antibodies akagadzirwa ane hunhu hwakasimba uye kugadzikana kwakanaka. Midziyo yakawanda yekuchenesa ma antibodies inogona kushandiswa kupa masevhisi ekuchenesa ma antibodies kubva kunzvimbo dzakasiyana siyana dzakadai setsuro, gwai, huku, mbeva monoclonal antibodies, pamwe ne protein A/G affinity cleansing uye masevhisi ekuparadzanisa ma antibodies.
Zvichibva panzira dzakasiyana-siyana dzekugadzirira ma antibodies dzakadai se hybridoma cell technology, Alpha Lifetech inogona kupa masevhisi akagadzirirwa ma antibodies e monoclonal ne polyclonal emarudzi akasiyana. Pamusoro pezvo, mabasa akasiyana-siyana kubva pakutevedzana kwe high throughput, kugadzirira ma antibodies, kuchenesa ma antibodies kusvika pakusimbiswa kwe antibody anogonawo kupihwa. Tichasarudza nzira yekuchenesa zvichienderana nechinangwa chaicho chemutengi. Zvichibva pahurongwa hwakakwana hwema platform akadai se platform yekugadzira ma antibodies ne protein platform, Alpha Lifetech inovhara masevhisi ekumusoro nekudzika ekugadzira ma antibodies, ichipa kuchenesa ma antibodies, kutevedzana kwe high throughput antibody, kusimbiswa kwe antibody nemamwe masevhisi ehunyanzvi.
Tekinoroji yeHybridoma
Tekinoroji yeHybridoma inogona kushandiswa kugadzira ma antibodies emonoclonal. Nekubatanidza masero espleen kubva kumakonzo akadzivirirwa nemasero emyeloma kubva kuvanhu kana mbeva, masero ehybridoma anoumbwa uye anoburitsa ma antibodies chaiwo. Ma antibodies emonoclonal anogadzirwa anobva kumbeva anogona kushandiswa mukutsvagisa kwesainzi senge enzyme-linked immunosorbent assay, radioimmunoassay, immunohistochemistry, flow cytometry, protein purification, nezvimwewo. Samples dzeMonoclonal antibody dzinosanganisira ascites, hybridoma supernatant, nezvimwewo. Sampuro inowanikwa nekupinza masero ehybridoma mudumbu remhuka uye kushandisa physiological environment yedumbu remhuka kugadzira ma antibodies inonzi ascites sample. Masero eHybridoma anopinzwa mudumbu remhuka anogona kuwanda nekuburitsa ma antibodies emonoclonal, ayo anogona kuungana mu ascites. Nekuda kwekugona kwetsvina (mapuroteni emhuka, mahormone, nezvimwewo) muma antibodies aya, kucheneswa kunodiwa. Zvichibva pabasa rekuburitsa maB lymphocytes, ma antibodies emonoclonal anowanzo buditswa munzvimbo yekurera pakurima masero ehybridoma. Mvura ine monoclonal antibodies iri mu culture medium inonzi hybridoma supernatant. Kuchena kwema monoclonal antibodies ari mu supernatant kwakanyanya, asi kucheneswa kwakawanda kunodiwa kuti pave nekugadzirira kukuru sezvo anowanikwa kuburikidza ne in vitro culture.
Kucheneswa kweanodzivirira muviri kunogona kuparadzanisa ma antibodies emhando yepamusoro kubva kuma complexes. Parizvino, nzira dzekuchenesa affinity uye kupatsanura ma antibodies ndidzo dzinowanzo shandiswa, kunyanya dzinosanganisira protein A/G purification, ion exchange chromatography, gel filtration, precipitation uye hydrophobic interaction chromatography. Pakati padzo, protein A/G affinity purification service inozivikanwa zvikuru mukushandiswa kwakasiyana-siyana kwe antibodies. Maitiro eAlpha Lifetech ekugadzira ma antibodies e monoclonal akavakirwa pa hybridoma technology anoratidzwa muMufananidzo 1.
Mufananidzo 1 Dhayagiramu yemaitiro ekugadzira ma antibodies e monoclonal zvichibva pa hybridoma technology.
Tekinoroji yeAntibody Drug Conjugate uye Hybridoma
Antibody drug conjugate (ADC) chinhu chitsva chebiological chinobatanidza ma antibodies nemishonga inouraya ma cytotoxic. Antibody drug conjugate inosanganisa kugona kwekuziva ma antibodies nemhedzisiro ine simba yekuuraya mishonga inouraya ma cytotoxic, uye inoendesa mishonga inouraya ma cytotoxic mumasero egomarara kuburikidza nema antibodies, nokudaro ichiwana kurapwa kwakarurama. Chikamu cheantibody mumishonga yeADC chinowanzo gadzirwa kuburikidza ne hybridoma technology kana dzimwe nzira dzekugadzira ma antibody, dzakadai se genetic engineering antibody technology. Hybridoma technology inopa zvinhu zvepamusoro zve antibody zvemishonga yeADC. Mishonga yeADC nema monoclonal antibodies akagadzirwa kuburikidza ne hybridoma technology anoita basa rakakosha muminda yakaita sekurapa kenza. Anogona kushandiswa ari ega kana pamwe chete kuti awedzere kushanda zvakanaka kwekurapa.
Ma antibodies eMonoclonal akagadzirwa ne hybridoma tekinoroji ane hunyanzvi hwakanyanya, anogona kusunga nekuziva maantigen chaiwo nemazvo, uye anogona kugadzirwa pamwero mukuru. Kuburikidza ne hybridoma sequencing technology, ma antibodies majini mu hybridoma cells anogona kutorwa nekurongwa kuti awane sequences dzinoenderana, izvo zvinobatsira pakuratidzwa kwe antibody kunotevera. Ma antibodies eMonoclonal akagadzirwa kuburikidza ne hybridoma technology anogona kushandiswa kuona zviratidzo zvechirwere, zvakaita se bundu markers, viral antigens, nezvimwewo, zvichipa rutsigiro rwakasimba rwekuongororwa kwezvirwere nekukurumidza. Pamusoro pezvo, inogona zvakare kushandiswa mukuyedza kwakadai se immunoblotting uye immunohistochemistry kudzidza mapuroteni expression, localization, structure, uye functionality.
tekinoroji yehybridoma Kugadzira Antibody Service Workflow
| Matanho | Zviri Mukati meSevhisi | Nguva yekutevera nguva |
|---|---|---|
| Danho 1: Kudzivirira mhuka | (1) Kudzivirira mhuka ka4, kuwedzera njodzi yekukuvadza mhuka ka1, huwandu hwese hwemadosi mashanu akapihwa. (2) Seramu isina kunaka yakatorwa vasati vabaiwa majekiseni, uye ELISA yakaitwa padosi yechina kuti ionekwe seramu titer. (3) Kana serum antibody titer yemuyero wechina ikasangana nezvinodiwa, imwe dose yekudzivirira ichapihwa mazuva manomwe ropa risati ratorwa. Kana ikasasangana nezvinodiwa, jekiseni rekudzivirira richaramba richienderera mberi. (4) Simba rakakodzera, masero espleen anotsaurwa kuti masero asanganiswe. | Mavhiki gumi kusvika gumi nemaviri |
| Danho rechipiri: Kusanganiswa uye Kuongororwa | (1) Kusanganiswa: Mushure mekubaiwa nhomba, masero etsvina yembeva akaunganidzwa ndokubatanidzwa nemasero emyeloma. (2) Kuwanda uye kuongororwa: Masero ehybridoma akawanikwa akaongororwa kuti awedzere kukura uye ELISA yakaongororwa kuti pave nema clones anoshanda zvakanaka. | Mavhiki matanhatu |
| Danho rechitatu: Subclone | Kubvisa masero emuviri ane 15-25 akanaka uchishandisa nzira yakaganhurirwa yekudiridza, uye pakupedzisira kuona 10-15 akanaka. | Mavhiki matatu kusvika mana |
| Danho rechina: Kugadzirwa kweAscites uye Kucheneswa kweAntibody | (1) Kugadzirwa kweAscites: Mhando gumi kusvika gumi neshanu dzehybridoma dzinoshandiswa pakugadzirwa kweascites mumakonzo (kana huwandu hwakawanda hwemhando dzehybridoma hukawanikwa, huchatumirwa muchimiro chemasero) (2) Kuzivikanwa kweAscites: Kuonekwa kweAscites ELISA, simba (protein/virusi>10 ^ 5, molecule diki/peptide>10 ^ 4) yekuchenesa pasi (3) Kucheneswa kweProtein A/G: Kucheneswa kweAscites | Mavhiki matatu kusvika mana |
| Danho rechishanu: Kutumira | (1) Ipa marudzi matatu kusvika mashanu e hybridoma antibody monoclonal. (2) Sero rega rega rehybridoma rinopa 1mg yeantibody yakacheneswa (kucheneswa kweascites). (3) Mushumo weMaitiro Ekuyedza | Vhiki imwe chete |
Mhando dzakasiyana-siyana
Dziviriro yezvirwere inogona kupihwa kumhuka dzakasiyana-siyana, kusanganisira mbeva, tsuro, makwai, nezvimwewo asi kwete chete.
Chinangwa Chakawanda
Zvichibva pahunyanzvi hwehybridoma, mabasa akawanda ekuwana maantibody anowanikwa anowanikwa: mapuroteni, mapeptide, mamorekuru madiki, mavhairasi, mapuroteni emembrane, mRNA, nezvimwewo.
Zvinyorwa zveKutevedza
Maitiro ekudzora mhando yeQC (simba rekudzivirira muviri, kudzora mhando yePBMC, kudzora mhando yeraibhurari, uye kuongorora hunhu hwekuongorora), mishumo yekuyedza yeChirungu, zvinyorwa zvekutanga zvekuyedza
Purogiramu Yakagadzirirwa Munhu Wako
Kugadzirisa purogiramu yemunhu mumwe nemumwe kuti ienderane nezvinodiwa nevatengi vakasiyana-siyana pakutsvaga ruzivo
Basa rekugadzira maAntibody eMonoclonal
Tinogona kupa masevhisi ekugadzira maantibody emhando yepamusoro, akachena uye akanyatsotsanangurwa, kusanganisira kugadzirwa kwemaantibody emonoclonal embeva uye maantibody emonoclonal etsuro.
Puratifomu yeTekinoroji yeHybridoma
Kusanganisira chirongwa chekudzivirira, masevhisi ekugadzirira maantibody, kucheneswa kwemaantibody, kutevedzana kwe antibody high throughput, kusimbiswa kwemaantibody, nezvimwewo.
Puratifomu Yekuronga Masero Ega Ega
Alpha Lifetech ine zvakanakira munguva yekuongorora uye kuwana ma antibodies emhando yepamusoro. Inogona kupa dhizaini yeantigen, synthesis, uye modification, dziviriro yemhuka, kuongorora kuwedzera kwemasero eB rimwe chete, uye kutevedzana kwemasero rimwe chete.
Chikuva Chekugadzira Antibody Display
Alpha Lifetech inogona kupa masevhisi ehunyanzvi ekugadzira maantibody ekuratidza phage kubva pakugadzira maantibody, kuchenesa maantibody, kutevedzana kwemaantibody, nezvimwewo.
Zvishandiso zveTekinoroji Zvakafanana
Nhanganyaya yeHybridoma TechnologyVHIDHIYO
Kana muine mibvunzo, ndapota inzwa wakasununguka kutibata chero nguva.
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2018-07-16 
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