Basa rekuchenesa mapuroteni emudumbu

Alpha Lifetech yakabudirira kugadzirira zviuru zvemapuroteni akabatana (recombinant proteins) evatengi vedu, anosanganisira ma antibodies akabatana (recombinant antibodies), mapuroteni akabudiswa (secreted proteins), mapuroteni e transmembrane (transmembrane proteins), mapuroteni (proteases), nezvimwewo, zvine mwero wekubudirira kukuru. Vatengi vanongoda kupa mapuroteni, CDS kana zita remapuroteni, uye Alpha Lifetech inogona kugadzira hurongwa hwakakwana hwekubatanidza mapuroteni uye kuchenesa munguva pfupi. Tinogona kupa masevhisi ekugadzira mapuroteni akagadzirwa zvichienderana nehurongwa hukuru hukuru hwekugadzira mapuroteni, uye kusarudza nzira dzakasiyana dzekugadzira mapuroteni akabatana uye kuchenesa zvinoenderana nezvinodiwa nevatengi.

Alpha Lifetech ine michina yakasiyana-siyana yekuchenesa mapuroteni, iyo inogona kupa masevhisi ekuchenesa mapuroteni echisikigo uye ekuchenesa mapuroteni akasanganiswa, akadai sekuchenesa affinity, molecular sieve chromatography, ion exchange chromatography, uye hydrophobic chromatography. Tichasarudza nzira dzekuchenesa zvinoenderana nezvinodiwa nevatengi vedu, uye todzisanganisa nepuratifomu yedu yekuchenesa mapuroteni kuti tiwane zvigadzirwa zvemapuroteni emhando yepamusoro munguva pfupi.

Kurongwa kweMapuroteni eMembrane

Zvichienderana nekupararira kwemapuroteni mumembrane uye hukama huripo pakati pemapuroteni emembrane ne membrane lipids, mapuroteni e membrane akakamurwa kuita zvikamu zvitatu, zvinosanganisira mapuroteni e peripheral membrane, mapuroteni e membrane akabatanidzwa, uye mapuroteni anonamira lipid.

Mapuroteni eIonic channel

Ionic channel protein iprotein ye membrane yakabatanidzwa inopinda mu membranes dzemuviri kuti igadzire maumbirwo chaiwo e channel, zvichibvumira ma ions mamwe kutakurwa kuburikidza ne membranes mukati nekunze kwesero, ukuwo ichidzivirira mamwe ma ions kuti asapfuure nepakati. Kusarudzwa kwema ion channel proteins kwakakosha pakuchengetedza mabasa ehupenyu hwemasero akadai sekusiyana kwehuwandu hwe ion pakati pemukati nekunze kwesero, kutakurwa kwe neurotransmitter, nezvimwewo.

G protein receptors dzakabatana

Mapuroteni akabatana eG protein izwi rinowanzo shandiswa kureva boka guru remapuroteni emembrane, ayo ari mareceptor akawanda epamusoro pemasero mu eukaryotes. Basa guru remapuroteni akabatana eG protein nderekutumira ruzivo rwekunze kwemasero mukati memasero kuburikidza nekubatana neproteni yeG, zvichiita basa rakakosha mukutumira masaini. Mapuroteni akabatana eG protein anoona uye anosunga kuma ligands ekunze kwemasero kuti atange nzira dzekutumira masaini mukati memasero, nokudaro achidzora mabasa akasiyana-siyana emuviri emasero.

Zvidimbu Zvakaita Semavhairasi

Chidimbu chakaita sevhairasi ipuroteni inoumbwa nekuzviunganidza kwepuroteni imwe kana akawanda evhairasi. Zvidimbu izvi zvakafanana muchimiro uye maumbirwo ezvidimbu zvevhairasi, asi hazvina majini evhairasi, saka hazvitapukiri.

Zvikamu Zvakaita Semavhairasi zvinogona kushanda senzvimbo yekuratidza mapuroteni emukati memuviri, zvichitevedzera kuumbwa uye kushanda kwemapuroteni emukati memuviri munzvimbo dzakasikwa nekuratidza mapuroteni chaiwo emukati memuviri paZvikamu Zvakaita Semavhairasi. Mukugadzirwa kwemishonga yekudzivirira, maantigen anodzivirira ehutachiona, kazhinji mapuroteni emukati memuviri, anogona kuratidzwa paZvikamu Zvakaita Semavhairasi kuti akurudzire muviri kuti ugadzire mhinduro dzekudzivirira muviri kurwisa maantigen aya. Mishonga yekudzivirira inogadzirwa nenzira iyi yakachengeteka zvikuru uye inodzivirira muviri kubva kuzvirwere.

Kucheneswa kweMapuroteni eMembrane

Kune nzira dzakasiyana-siyana dzekuchenesa mapuroteni emembrane, pakati padzo affinity chromatography inoshandisa affinity yemapuroteni emembrane pachawo kana fusion tags, dzakadai seHis tags, nema ligands avo chaiwo kuti vawane membrane protein cleansing and separation. Ion exchange chromatography inoshandisa charge properties yemapuroteni emembrane kuti iaparadzanise pama ion exchange resins. Zvisinei, membrane protein solutions dzinowanzova ne detergents, uye relaxing inogona kukanganisa kushanda kwe ion exchange chromatography. Gel filtration chromatography ndeyekushandisa mutsauko we membrane protein molecular weight kuti iparadzanise pa gel column, kuburikidza nenzira iyi, tinogona kuwana high purity membrane protein. Nekuda kwe affinity uye hydrophobicity yemapuroteni e membrane, anogona kupatsanurwa pa chromatographic columns. Iyi nzira inoshanda, inokurumidza, uye inonyanya kunzwa, asi mutengo wacho wakakwirawo.

kucheneswa kweprotein yemembrane - Alpha Lifetech

Mufananidzo 1. Dhayagiramu yepfungwa yekucheneswa kweprotein affinity

Mabhenefiti ekuchenesa mapuroteni emukatiNEI kusarudza

Kugadziriswa kweCodon

Kubuda kwemapuroteni akawanda anonyungudika uye kushanda kwemapuroteni akawanda kunogona kuvimbiswa

Nzira dzekuvirisa mapuroteni akawanda: diki-diki (1L, 10L, uye 30L), hombe-diki (80L, 130L, 250L, uye 500L matangi ekuvirisa)

Kugadzirwa kwemapuroteni akagadzirwazve muchimiro che "high-purity recombinant protein" mumamirigiramu nemagiramu munguva pfupi

Endotoxin yakaderera: Nzira yekuona LAL

Sisitimu yakakwana yekutsigira magwaro eGMP inoita kuti zvinhu zvese, ma reagents, uye ruzivo rwekugadzirira chigadzirwa zvinyatsoteverwa.

Dzokera kuPeji rePuratifomu reMapuroteni eMembrane

Kana muine mibvunzo, ndapota inzwa wakasununguka kutibata chero nguva.

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