Chikuva Chekugadzira Antibody Display yePhage Display

Alpha Lifetech, ine ruzivo rwakakura mukutsvaga maantibody, inogona kupa masevhisi ekudzivirira maantibody emhando dzakasiyana-siyana, pamwe nekuvaka uye kuongorora maraibhurari ekudzivirira maantibody. Alpha Lifetech inogona kupa vatengi zvigadzirwa zve antibody zvine hungwaru uye recombinant antibody, pamwe nemabasa anoenderana, kugadzirira maantibody anoshanda zvakanaka, akanyatsotsanangurwa, uye akasimba. Nekushandisa mapuratifomu ekudzivirira maantibody akakwana uye mainjiniya ekudzivirira maantibody, tinopa masevhisi anofukidza kugadzirwa kweantibody kumusoro nekudzika, kusanganisira masevhisi ehunyanzvi akadai sekugadzirira maantibody, kuchenesa, kutevedzana kweantibody, uye kusimbiswa.

Alpha Lifetech ine puratifomu yakakura yekuwana maantibody inopa vatengi mabasa akasiyana-siyana anosanganisira kudzivirira mhuka, kuvaka uye kuongorora raibhurari ye phage display antibody, kutevedzana kweantibody, kuratidza maantibody, kucheneswa kweantibody, kusimbiswa kweantibody, uye kunyora maantibody zvichibva pa hybridoma technology, single B cell technology, phage display technology, nezvimwewo. Alpha Lifetech ine standard production line, uye pamusoro pema antibody preparation services, anotsigira masevhisi akadai se antibody humanization, antibody affinity maturation, ADC drug design and development, uye CAR-T postward sequence design inogonawo kupihwa. Panguva imwe chete, Alpha Lifetech yakagadzira antibody library yakavakirwa pa M13, T4, uye T7 phage display technology platforms, ine storage capacity inosvika 10^8-10^9. positive rate, insertion rate, uye diversity ye library zvese zvinogona kusvika pamusoro pe90%.

Tekinoroji yekuratidza maphage

Kugadzirwa kwema antibodies emonoclonal kwakagadzirwa pakutanga uchishandisa tekinoroji ye hybridoma monoclonal antibody kugadzira ma antibodies emonoclonal emouse hybridoma. Kunyanya, nekubatanidza masero espleen kubva kumakonzo akapihwa majekiseni nemasero e myeloma kubva kuvanhu kana mbeva, masero e hybridoma anoumbwa, ayo anoburitsa ma antibodies chaiwo e hybridoma monoclonal antibody. Nekushandurwa kwema antibodies emonoclonal emouse akagadzirwa nemunhu uye kugadziriswa kwema genetic engineering ema antibodies kuti ape nzvimbo inogara iripo ye immunoglobulin yemunhu, immune genicity yavo inogona kuderedzwa. Kugadzirwa kwema antibodies emonoclonal kunoshandiswa zvakanyanya mukushandiswa kwe biomedical uye kliniki.

Alpha Lifetech inogona kupa masevhisi ekugadzira maantibody kubva pakugadzira protocol yekutsvagisa kwepamberi pekiriniki kusvika pakuwana maantibody drug conjugates (ADCs) uye kusimbiswa kwemhuka. Maantibody drug conjugates (ADCs) anogona kuendesa mishonga yechemotherapy kumasero ekenza. Mushure mekusunga kune zvinangwa zvakananga zvinoratidzwa pamasero ekenza, ADC inoburitsa mishonga ine chepfu mumasero ekenza. Alpha Lifetech inogonawo kupa vatengi masevhisi ekukura kweantibody affinity. Nekuvandudza mutation optimization uye high-throughput phage display screening technology, maantibody ane some affinity anotanga aongororwa, uye ipapo amino acid mutations inounzwa kuti igadzire mhando dzakasiyana. Mushure meizvozvo, maantibody ane affinity akakwira akaongororwa uye akaongororwa uchishandisa nzira dzekuongorora. Mushure mematanho akawanda ekugadzirisa uye kuongorora chimiro, maantibody ane affinity yakakwira uye specificity yakasimba pakupedzisira akawanikwa.

Alpha Lifetech inogona kuvaka mhando dzakasiyana dzemaraibhurari ekuratidza maantibody phage, kusanganisira maraibhurari ekudzivirira muviri, maraibhurari emuno, maraibhurari e semi synthetic, uye maraibhurari ekugadzira. Zvichibva pakukwanisa kukuru kwemaaraibhurari ekudzivirira muviri, maantibody chaiwo anogona kuwanikwa. Ma phagemid vectors akawanda akadai se pMECS, pComb3X, uye pCANTAB 5E anogona kupihwa, pamwe chete nemarudzi akadai se TG1 E. coli, XL1 Blue, uye ER2738. Kuwedzera pakukwanisa kwemaraibhurari kusvika ku10^9, mwero wekupinza zvidimbu zveraibhurari wakakwirawo, zvichigadzira mamiriro akakwana ekuongorora ma antibodies anogutsa vatengi. Chati yemafambiro ebasa rekugadzirira maantibody eAlpha Lifetech yakavakirwa patekinoroji yekuratidza ma phage inoratidzwa muMufananidzo 1.

Mufananidzo 1 Dhayagiramu yemaitiro ekugadzira ma antibodies e monoclonal zvichibva pahunyanzvi hwekuratidza phage.

Kufamba Kwebasa Rekugadzira MaAntibody ePhage Display

Matanho	Zviri Mukati meSevhisi	Nguva yekutevera nguva
Danho 1: Kudzivirira mhuka	(1) Kudzivirira mhuka ka4, kuwedzera njodzi yekukuvadza mhuka ka1, huwandu hwese hwemadosi mashanu akapihwa. (2) Seramu isina kunaka yakatorwa vasati vabaiwa majekiseni, uye ELISA yakaitwa padosi yechina kuti ionekwe seramu titer. (3) Kana serum antibody titer yemuyero wechina ikasangana nezvinodiwa, imwe dose yekudzivirira ichapihwa mazuva manomwe ropa risati ratorwa. Kana ikasasangana nezvinodiwa, jekiseni rekudzivirira richaramba richienderera mberi. (4) Simba rakakodzera, kuunganidzwa kweropa uye kupatsanurwa kwemamonocytes	Mavhiki gumi
Danho rechipiri: Kugadzirira cDNA	(1) PBMC Total RNA Extraction (RNA Extraction Kit) (2) Kugadzirira kwe cDNA ne RT-PCR kwakavimbika (reverse transcription kit)	Zuva rimwe chete
Danho rechitatu: Kuvaka Raibhurari yeAntibody	(1) Uchishandisa cDNA se template, majini akawedzerwa nePCR kaviri. (2) Kugadzirwa kwePhage uye kushandurwa: gene splicing phagemid vector, electroporation shanduko yeTG1 host bacteria, kuvakwa kwe antibody library. (3) Kuzivikanwa: Sarudza ma clones makumi maviri nemana zvisina tsarukano, PCR identification positive rate + insertion rate. (4) Kugadzirira kwe phage: M13 phage amplification+cleansing. (5) Kununura raibhurari yekuratidza maPhage	Mavhiki matatu kusvika mana
Danho rechina: Kuongororwa kweRaibhurari yeAntibody (zvikamu zvitatu)	(1) Kuongororwa kwemuviri kwezvikamu zvitatu (solid-phase screening): kuongorora kumanikidzwa kubvisa ma antibodies asiri chaiwo kusvika padanho rakakwana. (2) Bacteriophage yakasarudzwa seimwe chete yekuwedzera huwandu hwemasero eropa+IPTG induced expression+ELISA yekuona ma clones akanaka. (3) Ma clones ese akanaka akasarudzwa kuti aongorore majini.	Mavhiki mana kusvika mashanu

Kubatsira kweTekinoroji yePhage Display

Alpha Lifetech ine ruzivo rwakakura mukugadzira maantibody. Mumakore apfuura, Alpha Lifetech yakagadzira puratifomu yakazara yekugadzira maantibody.

Mabasa Ekutsigira

Tinogona kupa mabasa akasiyana-siyana ekuvaka maraibhurari ekudzivirira muviri emhuka uye masevhisi ekuongorora maraibhurari ekudzivirira muviri zvichienderana nezvinodiwa nevatengi.

Chinangwa Chakawanda

Masevhisi akawanda ekuwana maantibody anowanikwa: mapuroteni, mapeptide, mamorekuru madiki, mavhairasi, mapuroteni emembrane, mRNA, nezvimwewo.

Maveji Akawanda

Basa rekuvaka raibhurari rakasiyana-siyana, tinogona kupa mavectors akasiyana-siyana ebacteriophage anosanganisira PMECS, pComb3X, uye pCANTAB 5E, uye toagadzirisa zvichienderana nezvinodiwa nevatengi.

Puratifomu Yevanhu Vakuru

Kugona kuchengetwa kunogona kusvika 10 ^ 8-10 ^ 9, mwero wekuisa uri pamusoro pe90%, uye hukama hwema antibodies anowanikwa kuburikidza nekuongorora hunowanzo kuve padanho re nM pM.

BASA RINE HUMWE

Maitiro Akawanda Ekugadzira MaAntibody

Basa rekugadzira maAntibody eMonoclonal

Puratifomu yeTekinoroji yeHybridoma

Puratifomu Yekuronga Masero Ega Ega

Chikuva Chekugadzira Antibody Display yePhage Display

Kugadzirwa kwemasoja ekudzivirira chirwere emonoclonal - Alpha Lifetech

Basa rekugadzira maAntibody eMonoclonal

Tinogona kupa masevhisi ekugadzira maantibody emhando yepamusoro, akachena uye akanyatsotsanangurwa, kusanganisira kugadzirwa kwemaantibody emonoclonal embeva uye maantibody emonoclonal etsuro.

Puratifomu yeTekinoroji yeHybridoma

Kusanganisira chirongwa chekudzivirira, masevhisi ekugadzirira maantibody, kucheneswa kwemaantibody, kutevedzana kwe antibody high throughput, kusimbiswa kwemaantibody, nezvimwewo.

Puratifomu Yekuronga Masero Ega Ega

Alpha Lifetech ine zvakanakira munguva yekuongorora uye kuwana ma antibodies emhando yepamusoro. Inogona kupa dhizaini yeantigen, synthesis, uye modification, dziviriro yemhuka, kuongorora kuwedzera kwemasero eB rimwe chete, uye kutevedzana kwemasero rimwe chete.