Phage Ratidza Antibody Development Platform

Alpha Lifetech, ine ruzivo rwayo rwakakura rweprojekiti mukuwanikwa kweantibody, inogona kupa yakagadziridzwa monoclonal uye polyclonal antibody masevhisi emarudzi akawanda, pamwe neanodzivirira raibhurari yekuvaka uye yekuongorora masevhisi. Alpha Lifetech inogona kupa vatengi mhando yakavimbiswa antibody uye recombinant antibody zvigadzirwa, pamwe neanoenderana masevhisi, kugadzirira anoshanda, akanyatso tarisa, uye akagadzikana masoja ekudzivirira chirwere. Nekushandisa yakazara antibody mapuratifomu uye antibody engineering, isu tinopa masevhisi anofukidza kumusoro nekudzika kwekugadzirwa kweanorwisa utachiona, anosanganisira masevhisi ehunyanzvi akadai sekugadzirira antibody, kucheneswa, kutevedzana kwevanhu, uye kusimbiswa.

Alpha Lifetech ine chikuva chekutsvagisa chikuva chinopa vatengi masevhisi akasiyana siyana anosanganisira kusadzivirirwa kwemhuka, phage inoratidzira antibody raibhurari kuvaka uye kuongorora, kutevedzana kweanodzivirira, antibody kutaura, kucheneswa kweantibody, kusimbiswa kweantibody, uye antibody labeling zvichienderana neiyo hybridoma tekinoroji, imwechete B cell tekinoroji, phage kuratidza tekinoroji, nezvimwe. Alpha Lifetech ine mutsara wekugadzira wakajairika, uye mukuwedzera kune antibody kugadzirira masevhisi, anotsigira masevhisi akadai seanorwisa munhu, antibody affinity maturation, ADC dhizaini dhizaini uye kusimudzira, uye CAR-T inotevera kutevedzana dhizaini inogona kupihwa zvakare. Panguva imwecheteyo, Alpha Lifetech yakavaka raibhurari inodzivirira vanhu kubva paM13, T4, uye T7 phage kuratidza tekinoroji mapuratifomu, ine dura rekuchengetedza rinosvika 10 ^ 8-10 ^ 9. Mwero wakanaka, chiyero chekuisa, uye kusiyana kweraibhurari zvese zvinogona kusvika pamusoro pe90%.

Phage kuratidza Technology

Kugadzirwa kwemonoclonal antibodies kwakatanga kugadzirirwa uchishandisa tekinoroji yehybridoma monoclonal antibody kugadzira mbeva hybridoma monoclonal antibody. Kunyanya, nekubatanidza spleen masero kubva kumajekiseni ane myeloma masero kubva kuvanhu kana mbeva, masero ehybridoma anoumbwa, anoburitsa chaiwo hybridoma monoclonal antibody antibodies. Nekugadziridzwa kwemunhu kwembeva monoclonal antibodies uye genetic engineering kugadziridzwa kwemasoja ekudzivirira chirwere kuti avape inogara yemunhu immunoglobulin dunhu, immunogenicity yavo inogona kuderedzwa. Monoclonal antibody engineering inoshandiswa zvakanyanya mu biomedical uye kiriniki application.

Alpha Lifetech inogona kupa imwechete-stop antibody engineering masevhisi kubva preclinical research protocol dhizaini kune antibody drug conjugates (ADCs) kuwanikwa uye kusimbiswa kwemhuka. Antibody drug conjugates (ADCs) inogona kuendesa chemotherapy mishonga kumasero egomarara. Mushure mekusunga kune zvakananga zvinangwa zvinoratidzwa pamasero egomarara, ADC inoburitsa cytotoxic mishonga mumasero egomarara. Alpha Lifetech inogonawo kupa vatengi vane yakazara antibody affinity maturation masevhisi. Nekukwidziridzwa kwepamusoro-soro uye tekinoroji yekutarisa-yepamusoro-soro phage, masoja ekudzivirira chirwere ane humwe hukama anotanga kuongororwa, uyezve maamino acid ekuchinja anounzwa kuti abudise akasiyana akasiyana. Mushure mezvo, masoja ekudzivirira epamusoro akaongororwa uye akaongororwa achishandisa nzira dzekuongorora. Mushure mekutenderera kwakawanda kwekugadzirisa uye kuongororwa kwemaitiro, masoja ekudzivirira chirwere ane hukama hwepamusoro uye akasimba chaiwo akazowanikwa.

Alpha Lifetech inogona kuvaka mhando dzakasiyana dzeantibody phage kuratidza maraibhurari, kusanganisira maraibhurari edziviriro, maraibhurari ekuzvarwa, semi synthetic raibhurari, uye maraibhurari ekugadzira. Zvichienderana nehupamhi hwemaraibhurari ekurwisa masoja ekudzivirira chirwere, masoja ekudzivirira chirwere anogona kuwanikwa. Multiple phagemid vectors akadai pMECS, pComb3X, uye pCANTAB 5E inogona kupihwa, pamwe nemhando dzakadai TG1 E. coli, XL1 Blue, uye ER2738. Pamusoro pehuwandu hweraibhurari hunosvika gumi ^9, mwero wekuiswa kwechidimbu cheraibhurari wakakwira zvakare, zvichigadzira mamiriro akakwana ekuongorora masoja ekudzivirira chirwere anogutsa vatengi. Iyo inoyerera yeAlpha Lifetech's antibody yekugadzirira sevhisi yakavakirwa pageji yekuratidzira tekinoroji inoratidzwa muMufananidzo 1.

Fig.1 dhiyabhorosi dhizaini yekugadzirira monoclonal antibodies zvichibva pane phage kuratidza tekinoroji.

Phage Display Antibody Production Workflow

Matanho	Zvemukati zveSevhisi	Nguva yakatarwa
Danho 1: kubayisa mhuka	(1) Mhuka yekudzivirira 4 nguva, booster yekudzivirira 1 dose, inokwana 5 doses yakabaiwa. (2) Negative serum isati yabatwa, uye ELISA yakaitwa pachiyero chechina kuti ione serum titer. (3) Kana iyo serum antibody titer yedosi yechina ikasangana nezvinodiwa, imwe yekuwedzera dhizi yekudzivirira ichapihwa mazuva manomwe ropa risati raunganidzwa. Kana ikasaita zvinodiwa, kubaya kwemajekiseni nguva dzose kunoenderera mberi. (4) Yakakodzera potency, kuunganidza ropa uye kupatsanurwa kwemamonocytes	mavhiki gumi
Danho 2: cDNA Kugadzirira	(1) PBMC Total RNA Extraction (RNA Extraction Kit) (2) Kutendeseka kwepamusoro RT-PCR kugadzirira kwecDNA (reverse transcription kit)	1 zuva
Nhanho 3: Kuvakwa kweAntibody Library	(1) Kushandisa cDNA setemplate, majini akakwidziridzwa nemaround maviri ePCR. (2) Phage kuvaka uye shanduko: gene splicing phagemid vector, electroporation shanduko yeTG1 host mabhakitiriya, kuvaka antibody raibhurari. (3) Chiziviso: Nenguva isipi sarudza makumi maviri nemana clones, PCR chiziviso chakanaka chiyero + chekuisa mwero. (4) Kubatsirwa kwegadziriro yephage: M13 phage amplification + kuchenesa. (5) Phage kuratidza raibhurari kununura	3-4 mavhiki
Nhanho 4: Antibody Raibhurari Yekuongorora (3 kutenderera)	(1) Default 3-round screening (solid-phase screening): kudzvinyirirwa kuongorora kubvisa ma antibodies asiri chaiwo kusvika pamwero mukuru unobvira. (2) Single clone amplification bacteriophage yakasarudzwa+IPTG induced expression+ELISA kuonekwa kweakanaka clones. (3) Makoni ose akanaka akasarudzwa kuti aite gene sequencing.	4-5 mavhiki

Advantage Of phage Ratidza Technology

Alpha Lifetech ine ruzivo rwakapfuma mumunda wekuvandudza antibody. Kwemakore, Alpha Lifetech yakagadzira yakazara antibody kusimudzira chikuva.

Support Services

Isu tinogona kupa akasiyana emhuka akavakirwa immune library raibhurari mabasa ekuvaka uye echisikigo antibody raibhurari yekuongorora masevhisi zvinoenderana nezvinodiwa nevatengi

Multiple Target

Multiple target antibody kuwanikwa masevhisi aripo: mapuroteni, peptides, mamorekuru madiki, mavhairasi, membrane mapuroteni, mRNA, nezvimwe.

Multiple Vectors

Yakasarudzika sevhisi yekuvaka raibhurari, tinogona kupa akasiyana bacteriophage mavheji anosanganisira PMECS, pComb3X, uye pCANTAB 5E, uye chinja iwo zvinoenderana nezvinodiwa nevatengi.

Mature Platform

Kuchengetedza kunogona kusvika 10 ^ 8-10 ^ 9, mazinga ekuisa ese ari pamusoro pe90%, uye hukama hwemasoja ekudzivirira chirwere anowanikwa kuburikidza nekuongorora hunowanzo panM pM level.

Inoenderana SERVICE

Multiple Antibody Development Strategies

Monoclonal Antibody Development Service

Hybridoma Technology Platform

Imwe B Cell Sorting Platform

Phage Ratidza Antibody Development Platform

monoclonal antibody kuvandudza-Alpha Lifetech

Monoclonal Antibody Development Service

Isu tinogona kupa emhando yepamusoro, yakakwirira-kuchena, uye yakanyanya kujeka monoclonal antibody kusimudzira masevhisi, kusanganisira kugadzirwa kwembeva monoclonal antibodies uye tsuro monoclonal antibodies.

Hybridoma Technology Platform

Kusanganisira chirongwa chekudzivirira, antibody kugadzirira masevhisi, antibody kucheneswa, antibody yakakwira throughput sequencing, antibody kusimbiswa, nezvimwe.

Imwe B Cell Sorting Platform

Alpha Lifetech ine zvakanakira mukutarisa nguva uye kuwana emhando yepamusoro masoja ekudzivirira chirwere. Inogona kupa antigen dhizaini, synthesis, uye gadziriso, kusadzivirirwa kwemhuka, imwechete B cell enrichment yekuongorora, imwe sero sequencing.