Chikuva chekuvaka raibhurari yePhage Display Peptide

Alpha Lifetech ine matekinoroji akawanda ekuvaka raibhurari yepeptide. Isu tinonyanya kupa sevhisi yekuvaka raibhurari yeT7 peptide uye sevhisi yekuvaka raibhurari yeM13 peptide zvichienderana nemhando dzephage; tinopa sevhisi yekuvaka raibhurari yepeptide yakatenderera 7 uye sevhisi yekuvaka raibhurari yepeptide 12 zvichienderana nehukuru hwemamorekuru epeptide, nezvimwewo. Tinopawo sevhisi yekuvaka raibhurari yepeptide yakagadziriswa. Tinopawo masevhisi ekugadzira nekugadzira peptide yakagadziriswa.

Tekinoroji yekuratidza phage senzira yekuisa mapeptide anoshanda ekunze muphage pamusoro pecapsid, kuchengetedza mashandiro eprotein kana peptide yekunze hakukanganisi kugona kwe recombinant phage kutapurira sero rinobata. Chikamu chega chega chephage chinogona kuratidza peptide imwe chete isina kurongeka pamusoro payo, uye kuunganidzwa kwephage dzese dzinoratidza mapeptide akasiyana kunoita raibhurari yekuratidza phage. Maraibhurari ekuratidza phage maturusi ane simba ekuratidza mapeptide akawanda akagadzirwa uye peptide chaiyo yekuongorora, uye ave achishandiswa zvakanyanya muminda yekuongorora antigenic epitope, kuongorora mishonga, uye kugadzira majekiseni.

Nhanganyaya yekuvaka raibhurari yePeptide

Kuvaka raibhurari yePeptide ndekwekuwedzera maraibhurari epeptide nekubatanidza ma peptide DNA sequences mumafomu akasiyana kune mapuroteni ephage shell anoenderana, uye kushandisa phage reassembly kuisa target peptide pamusoro pe phage. Kuvaka raibhurari yePeptide kwakavakirwa patekinoroji yekuratidza phage ndekwechikamu chebiosynthesis che peptide synthesis, uye kune nzira dzekugadzira peptide ye liquid-phase uye solid-phase peptide synthesis dziri pamusika. Parizvino, Alpha Lifetech ine matekinoroji akasiyana-siyana ekuvaka raibhurari yepeptide, ayo anogona kupa maraibhurari epeptide akagadziriswa kare uye maraibhurari epeptide akasarongerwa zvichienderana nehukuru hwema peptide molecules, kusanganisira kuvaka raibhurari ye7-peptide, kuvaka raibhurari ye12-peptide, kuvaka raibhurari ye15-peptide, kuvaka raibhurari yecyclic 7-peptide, uye kuvaka raibhurari yecyclic 12-peptide, uye inogonawo kugadziriswa kugadzira maraibhurari epeptide emhando dzakasiyana siyana uye mashandisirwo.

Kushandiswa kwePeptide Library Construction and Screening

Maraibhurari epeptide akarongwa nehunyanzvi hwekuratidza phage anogonesa kuwana mapeptide anoshanda uye anopa hukama huripo pakati pe phenotype (peptide yakaratidzwa) uye genotype (DNA yakanyoreswa); maraibhurari aya akakodzera maitiro ekuongorora anoparadzanisa maclone akabatana kubva kumaclone asina kusungwa nekuchenesa affinity. Maraibhurari epeptide anoratidzwa phage anogona kushandiswa pa (i) kutsanangura ma epitopes emasero emuviri (ii) kuongorora ma receptor- kana maprotein-bound bioactive peptides, ma peptides akabatana nezvinangwa zvisiri zveprotein, ma peptides chaiwo emasero kana nhengo, nezvimwewo uye (iii) kugadzira masisitimu ekupa mishonga anoshandiswa nepeptide.

Maitiro eRaibhurari yePeptide yeKuratidza Phage

Mufananidzo 1: Puratifomu yekuvaka yePeptide Library yePhage Display

Kushanda Kwekuvaka Peptide Library yePhage Display

Maitiro	Zviri Mukati meSevhisi	Nguva yekutevera nguva
1. Kugadzirwa kweNucleotide Sequence	Kugadzirwa kwezvikamu zve nucleotide zvisina kurongeka zvehurefu hwakatarwa nevatengi	Mavhiki maviri kusvika matatu
2. Kugadzira uye Kugaya	Polymerization uye kugaya kweDNA yakagadzirwa	Zuva rimwe chete
3. Kuvaka Maraibhurari eElectroconversion	Mabhakitiriya emugadziri weER2738 akagadzirwa, uye E. coli yakazoshandurwa neplasmid yakagadzirwa zvakanaka.	Vhiki imwe chete
4. Kukura kweRaibhurari yePeptide uye Kuonekwa kweKusiyana-siyana	Kuonekwa kwehukuru hweraibhurari yepeptide kuburikidza nekuverengwa kwekoloni uye kusiyana kweraibhurari yepeptide kuburikidza nekutevedzana	Mazuva maviri kusvika matatu
5. Kuvaka Raibhurari yePeptide yePhage Display	Nekutapukira muraibhurari yepeptidi yephage packaging	Vhiki imwe chete