Chikuva Chekuongorora Peptide Library yePhage Display
Alpha Lifetech inopa nzira yekuongorora mapuroteni, masero, uye tissue raibhurari yakagadzirwa kare.
TAURA NESU01
Chikuva Chekuongorora Peptide Library yePhage Display
Alpha Lifetech ine ruzivo rwakakura mukuvaka nekuongorora raibhurari ye peptide phage, ichipa rutsigiro rwakasimba rwehunyanzvi rwekuwana mishonga midiki yemamorekuru, kuongororwa kwekiriniki, kurapwa kwezvirwere uye kutsvagisa kwezvipenyu. Nevaongorori vakadzidza zvikuru uye vane ruzivo mukuongorora raibhurari ye phage display peptide, tanga tichitarisa pakuvaka nekuongorora raibhurari ye peptide phage ine hukama hwakakura kwemakore gumi, uye takaongorora ma peptide ane mashandisirwo ekiriniki kune vatengi vazhinji pasi rese, ayo anopfuura 98% ane hukama hwakanyanya uye hune hunyanzvi, izvo zvakabatsirawo mukufambira mberi kwemunda wepasi rose wezvekurapa.
Mapeptide akaongororwa nehunyanzvi hwe library ye phage display ane hukama hwakakura uye anogona kubatana zvakananga nemapuroteni anotarisirwa. Pamusoro pezvo, nzira dzekuongorora peptide library dzakagadzirwa kare dzine zvakanakira zvemutengo wakaderera wekugadzira uye kutenderera kwenguva pfupi kwekugadzirira. Zvakanakira mapeptide, akadai semamorekuru madiki, kupinda kwemuviri kwakawanda, kugadzikana kwakanyanya, kushaya simba kwemuviri uye chepfu, zvinoita kuti ashandiswe zvakanyanya mukutsvaga mishonga, kugadzira majekiseni, kuongororwa kwakanangana nekurapa kenza, kutsvaga nzira dzekutumira masaini emasero uye mamwe mabasa ekurapa.
mhando dzemaraibhurari epeptidi ekuratidza phage
Alpha Lifetech yakazvipira kugadzira kuvaka uye kuongorora maraibhurari e peptide display kune vatengi vedu vepasi rose kuti tive nechokwadi chekuti ma peptide sequences akakosha anoshanda anoongororwa mukati menguva yakatarwa. Tinogona kupa maraibhurari e peptide ane linear, maraibhurari e cyclic peptide uye mamwe maraibhurari e peptide asina kurongeka ane 5-25 amino acids., uye maraibhurari edu e peptide phage display ane simba remaraibhurari anopfuura 10^9, izvo zvinobatsira zvikuru pakuongorora ma peptide sequences akakodzera kuti agutse zvinodiwa nevatengi vedu. Kusvika pari zvino, takabudirira kuvaka maraibhurari e peptide ane linear 7, maraibhurari e peptide ane linear 9, maraibhurari e peptide ane linear 10, maraibhurari e peptide ane linear 15, maraibhurari e peptide ane linear 12, maraibhurari e peptide ane cyclic 7, uye mamwe maraibhurari e peptide ane huwandu hwakasiyana hweamino acid uye maumbirwo, kuitira kugutsa zvinodiwa zvakasiyana zvemasayendisiti pasi rese.
Raibhurari yemhando dzakasiyana-siyana dzephage peptide
Raibhurari yedu yepeptide inosanganisira mapeptide akatsetseka, mapeptide anotenderera, uye mapeptide akagadziriswa kuti ive nechokwadi chekuti zvinangwa zvinofukidzwa zvakanyanya uye zvakasiyana-siyana. Rudzi rweraibhurari yacho runochinjika uye rwakasiyana-siyana, izvo zvinogona kusangana nezvinodiwa pakugadzirwa kwemishonga, kuwanikwa kwezviratidzo zvekuongorora uye kuongororwa kwepeptide inoshanda.
Maitiro ekuongorora akangwara
Gadzirisa nzira yekuongorora zvinoenderana nehunhu hwechinangwa, pamwe chete nekuongorora kwechikamu chakasimba, kuongorora chikamu chemvura uye kuongorora masero kuti uve nechokwadi chekuti anonyatsojeka uye affinity yakakwirira.Kuongororwa nekuvandudzwa kwemasero akawanda, kutorwa nemazvo kwemapeptides ane mutengo wakakwira.
Kusimbisa uye kugadzirisa zvinobudirira
Kushandisa tekinoroji ye "high-throughput sequencing" uye "bioinformatics" kuti ikurumidze kuvhara "target sequence", kugadzirisa mhedzisiro yekuongorora, uye kuchengetedza nguva nemutengo weR & D.
Rutsigiro rwesevhisi yakazara
Kupa mhinduro imwe chete kubva pakuvaka raibhurari yepeptide kusvika pakusimbiswa kunotevera kutsigira kugadzirwa kwemishonga iri pasi uye tsvakiridzo yemaitiro emamorekuru.
Maitiro Ekuongorora Raibhurari yePeptide Display yePhage Display
Mufananidzo 1: Puratifomu Yekuongorora Peptide Library yePhage Display
Kufamba Kwebasa Rekuongorora Peptide Library yePhage Display
| Maitiro | Zviri Mukati meSevhisi | Nguva yekutevera nguva |
|---|---|---|
| 1. Kutapurirana kweTarget uye Kuchengetedzwa kweRaibhurari yePeptide | Mamorekuru akatarwa (akadai semapuroteni, ma antibodies kana masero) anomiswa kufamba pazvinotakura zve solid-phase uye anoiswa mu incubator nema phages mu peptide display library kuti asunge ma peptide chaiwo kune chinangwa. | Zuva rimwe chete |
| 2. Kugadzirisa uye Kuvandudza | Ma phage asina kutsanangurwa akabviswa ne pH yakaderera uye molecular elution inokwikwidzana, uye ma phage ane hukama hwakanyanya akapfuma. | Zuva rimwe chete |
| 3. Kuongororwa Kwenguva Yekuongorora | Mushure mekuongororwa kwe3-4, kuomarara kwemamiriro ekuongorora kwakavandudzika zvishoma nezvishoma, uye kujeka kwemapeptide akasarudzwa kune chinangwa kwakawedzerwa. | Vhiki imwe chete |
| 4. Kuongorora Kutevedzana kwePeptide | Ma phage akapfuma akaongororwa ne high-throughput sequencing kuti awane optimal peptide sequence. | Vhiki imwe chete |
Kana muine mibvunzo, ndapota inzwa wakasununguka kutibata chero nguva.
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2018-07-16 
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