Sisitimu Yekuratidza Mapeji
Alpha Lifetech yakavaka sisitimu yekuratidza Phage yakasimba inochengetedza nguva yekutsvaga kwesainzi kana kutsvagisa mapurojekiti uye inobatsira kugadzirwa kunotevera.
TAURA NESU01
Sisitimu Yekuratidza Mapeji
Alpha Lifetech yave ichishanda zvakanyanya muhunyanzvi hwekuratidza Phage kwemakore mazhinji. Alpha Lifetech yakavaka puratifomu yakakwana yehunyanzvi hwekuratidzira Phage iyo inochengetedza nguva yekutsvagisa kwesainzi kana kutsvagisa mapurojekiti uye inobatsira kugadzirwa kunotevera. Tine masisitimu akawanda ekuratidza phage anowanikwa kune vatengi ekusarudza kubva, anosanganisira M13 phage, T7 phage, T4 phage, uye λ phage display systems. Zvichienderana nezvinodiwa zvako zvekuyedza, unogona kusarudza sisitimu yekuratidza phage yakakodzera. Alpha Lifetech inogona zvakare kupa vatengi masevhisi akadai sekugadzira vhh antibody, kugadzirwa kwescfv antibody, uye kugadzirwa kweFab antibody.
Nhanganyaya yekuratidzwa kwePhage
Tekinoroji yekuratidza mabhakitiriya inoshandisa maphage (hutachiona hunokanganisa mabhakitiriya) kutsvaga mamorekuru anoshanda eprotein kana mapeptides chaiwo. Matekiniki ekuvaka library yePhage antibody anosanganisira kuvaka library yeFab antibody antibody, kuvaka library yeScFv antibody, kuvaka library yeVHH antibody, nezvimwewo. Zvichienderana nemarudzi emabacteriophages, anogona kukamurwa kuita bacteriophage m13, T7 bacteriophage, T4 bacteriophage, λ bacteriophage, nedzimwe bacteriophage. Zvichienderana nerudzi rwe library, inogona kukamurwa kuita random peptide library, cDNA library, antibody library, uye protein library. Tekinoroji yekuratidza mabhakitiriya iri nyore kushandisa uye isingadhuri kushandisa ine Fab phage display, scfv phage display, vhh phage display. Zvisinei, nzira iyi inoderedza kusiyana kwema molecular genetics mu library asingakwanise kuratidza ma sequences akareba zvakanyanya.
Tekinoroji yekuratidza phage inokwanisa kugadzira chisungo chepanyama pakati pemorekuru iri kuratidzwa neDNA sequence inoburitsa mamorekuru ari kuratidzwa, uye amino acid sequence ye conjugate chaiyo inogona kuonekwa ipapo ipapo neDNA sequencing yechidimbu chinodiwa mu phage genome.
Parizvino, tekinoroji yekuratidza ma phage ndiyo inoshandiswa zvakanyanya. Pakati pazvo, pane kubudirira kukuru mukutsvagisa nekugadzira majekiseni matsva (majekiseni ekugadzira asingadhuri uye anoshanda), kugadzirwa kwemishonga yekudzivirira ma antibodies (kuongorora ma enzyme inhibitors), kufambiswa kwezviratidzo zvemasero (kuongorora ma epitopes akaenzaniswa), uye kutsvagisa ma antigen epitopes (kugadzirira ma monoclonal antibodies).
Nheyo yekuratidzwa kwephage
Kuratidzwa kwePhage tekinoroji yemamorekuru yakavakirwa pakuchinja kwemajini kweDNA yephage, iyo inosunga chidimbu chakanangana neprotein yephage coat nokudaro ichiiratidza pamusoro pephage. Kutevedzana kweDNA yekunze kunounzwa munzvimbo chaiyo munucleotide sequence yegenome yephage iyo inochengetedza protein yephage coat (Mufananidzo 1).
Kana hutachiona hwephage hukaitika, phage gene inotanga kuoneka mukati mebhakitiriya, uye chidimbu chakaiswa chinoratidzwa pamusoro pephage semusanganiswa wegene rine chekuita neprotein yejasi uye cloned sequence.
Mufananidzo 1: Magadzirirwo eBacteriophage M13 virion uye musimboti wetekinoroji yekuratidza phage.(Mashoko ekutsvaga: Kuratidzwa kwePhage nedzimwe nzira dzekuratidza peptide | Ongororo dzeFEMS Microbiology | Oxford Academic (oup.com))
Masisitimu ekuratidza phage
Mafilamentous phages ndiwo anonyanya kushandiswa pakuratidza mafilaments, uye mafilaments ekuratidza mafilaments anogona kugadzirwa uchishandisa mamwe mafilaments. Mafilaments akadaro anogona kuratidza mapeptides akakura kana ma screen cDNA libraries zviri nani kupfuura mafilaments efilaments.
Mufananidzo 2 Mhando dzemabhakitiriya anoshandiswa pakuratidzwa kwephage.(Mashoko ekutsvaga: Kuratidzwa kwePhage nedzimwe nzira dzekuratidza peptide | Ongororo dzeFEMS Microbiology | Oxford Academic (oup.com))
Kubatsira kwePhage display system
Masisitimu ekuratidza ma phage anowanzo shandisa ma filamentous phages, anosanganisira M13, fd, uye f1. Zvichienzaniswa ne M13 phages, ma T7 bacteriophage phages anoumbwa ne double-stranded DNA, iyo inoratidza kugadzikana kukuru uye hainyanyi kuchinja panguva yekudzokororwa. T7 phage, semutakuri, inokura nekukurumidza uye inogona kuumba ma plaque mukati memaawa matatu, izvo zvinochengetedza nguva yakawanda ye cloning uye kuongorora.
Kuvakwa kweraibhurari yeT7 phage kwave kuchishandiswa pakuratidza majini pamusoro nepasiri pamusoro uye pakuvaka maraibhurari ekuratidza mapeptides, cDNA, genomic DNA, uye antibodies. Izvi zvinobvumira T7 phage kushandiswa kwete semushonga wekuuraya utachiona chete, asiwo pakuongorora mishonga mukuongorora nhengo (seuropi, chiropa, nemapapu), kuongorora maantibody, kutsvakurudza mashandiro emuviri, nezvimwewo. Inogona zvakare kushandiswa pakuongorora nekurapa zvirwere zvakasiyana-siyana.
Mamwe marudzi ebacteriophages
Phage inotapukira yakasarudzwa
Kusiyana nezviratidzo zvechinyakare zvephage, selective infective phage (SIP) inobatanidza kugadzirwa kwemapuroteni neligand zvakananga nehutachiona hwephage uye kuwedzera pasina chikonzero chematanho ekudzivirira.
Mufananidzo 3. Selectively infective phage (SIP).(Mashoko ekutsvaga: Kuratidzwa kwePhage nedzimwe nzira dzekuratidza peptide | Ongororo dzeFEMS Microbiology | Oxford Academic (oup.com))
Mapoka enzvimbo
Ma "landscape phages" anokonzerwa nezviuru zvema "foreign peptides" akarongwa akakomberedza tubular capsids mu "super-tight repeating patterns", uye "polyvalent display" pa "landscape phages" inoita kuti pave ne "high-affinity selection", saka hazvigoneke kusiyanisa pakati pe "high" ne "low" ye "peptide ligands".
Mufananidzo 4. Pfungwa yema "landscape phages". (Mashoko ekutsvaga: Kuratidzwa kwePhage nedzimwe nzira dzekuratidza peptide | Ongororo dzeFEMS Microbiology | Oxford Academic (oup.com))
Masisitimu ekuratidza ma phage akawanda
Sisitimu yekuratidza yeM13 phage, T7 phage, T4 phage, λ phage yekuti vatengi vasarudze kubva.
Kugona kuongorora zvinhu zvakawanda kuburikidza nemagetsi
Tinogona kushandisa masisitimu ekuratidza maphage kuti tivake nekukurumidza maraibhurari makuru ephage, uye tiwane kuyedza kuongorora zvinhu zvine simba guru.
Sevhisi yekuratidza phage yakagadzirirwa
Vatengi vanogona kugadzirisa masevhisi akazara zvichienderana nezvinodiwa neprojekiti, zvakaita sekuongorora ma antibodies ane hukama hwakanyanya, nezvimwewo.
Rudzi rwakakura rwekushandisa
Inoshandiswa muminda yakaita seinjiniya yeantibody, peptide neprotein engineering, kuwanikwa kwemishonga nekuvandudzwa kwayo, tsvakurudzo yekudyidzana kwemapuroteni, nezvimwewo.
Kana muine mibvunzo, ndapota inzwa wakasununguka kutibata chero nguva.
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2018-07-16 
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