Nhanganyaya kuMaraibhurari eAntibody Akagadzirwa

Raibhurari yeantibody yakagadzirwa, inozivikanwawo seraibhurari yeDe novo, inzira yekugadzira inoshandisa matekiniki akadai seDNA synthesized kana phage display kugadzira nekugadzira nzvimbo dzakasiyana dzeantibody, kusanganisira nzvimbo dzeframework neCDR, pasina kuvimba nemaraibhurari eantibody asina ruzivo.

Raibhurari yemaantibody emhando yeSemi-synthetic inogadzirwa nekubatanidza maraibhurari emaantibody anowanikwa zvakasikwa nemarudzi akasiyana-siyana ekugadzira. Kazhinji inosanganisira kugadzira maDNA oligonucleotides kuti agadzire nzvimbo dzakasiyana-siyana dzinoenderana (CDRs), idzo dzinozobatanidzwa nehurongwa hwemaantibody hunogara huripo hunobva kuzvinhu zvakasikwa zvakaita semasero eB evanhu kana emhuka. Iyo synthesized CDR inounza kusiyana-siyana muraibhurari, zvichikonzera maantibody anonanga ma epitopes akasiyana-siyana. Iyo semi-synthetic antibody raibhurari inopa bvumirano pakati pekusiyana-siyana kwechisikigo chemuviri uye kusiyana-siyana kunodzorwa kunowanikwa kuburikidza nenzira dzekugadzira.

Musiyano uripo pakati pemaraibhurari eantibody asina ruzivo neakagadzirwa unoenderana nekwakabva majini e immunoglobulin. Panguva iyi, musiyano uripo pakati pemaraibhurari eantibody asina ruzivo neakagadzirwa uri pakuti yekutanga ine chikamu chezvikamu zveantibody zvisina ruzivo, zvakaita secheni diki kana cheni inorema, uye chimwe chete chazvo chinogadzirwa mu vitro; nepo yakagadzirwa ichitorwa zvachose kubva mukugadzirwa kweakagadzirwa nePCR mu vitro.

Alpha Lifetech inogona kupa

Alpha Lifetech Inc.inogona kupa raibhurari yemaantibody akasanganiswa/akagadziriswa kubva kumhuka nevanhu zvichibva papuratifomu yedu yehunyanzvi yekuwana maantibody. Nemakore akawanda eruzivo mukugadzirisa majini nekuvaka raibhurari, maraibhurari akagadziriswa anogona kuvakwa anokwanisa kugadzira maantibody ekugadzira ane hukama uye akajeka kupfuura kugona kwemaantibody echisikigo.Alpha Lifetech'sNyanzvi dzinofara kuzivisa kuti tinogona kuvimbisa kubudirira kukuru mukuvaka raibhurari yemaantibody ine ma clones akazvimiririra ari pakati pe10^8 - 10^10.

Alpha Lifetech Inc.inodada nekupa mabasa akazara ekuvaka maraibhurari eantibody kuti isangane nezvinodiwa zvakasiyana-siyana zvevaongorori vepasi rose, kusanganisira scFv, Fab, VHH antibody, uye maraibhurari akagadzirwa. Chinangwa chedu ndechekunzwisisa nekuzadzisa zvinodiwa nevatengi vakasiyana uye kubatsira nematambudziko ari kuuya uye ari kubuda mubasa rekutsvagisa.

Maitiro Ekuvaka Maraibhurari Ekudzivirira Mabhakitiriya Akagadzirwa

Dhizaini
Chikamu chekugadzira chinosanganisira kusarudza ma antibodies frameworks uye nzvimbo dzinoona kuti maCDR anowirirana zvichienderana nemaitiro eantibody aripo kana data remaumbirwo. Ma antibodies eCDR anogonawo kugadzirwa kuti agadzire mashandiro chaiwo kana kuwedzera hunhu hwekubatanidza.

Kugadzira
DNA yakagadzirwa inochengetedza maantibody akagadzirwa, kusanganisira nzvimbo dzese dze framework neCDR, inogadzirwa uchishandisa nzira dzemakemikari kana enzyme.

Kuungana
Zvidimbu zveDNA zvakagadzirwa zvinounganidzwa kuita mavector ekuratidza maantibody uchishandisa matekiniki akadai sePCR, ligation, kana Gibson assembly. Mavector aya anogona kuiswa musystem dzekuratidza maantibody akadai semabhakitiriya, mbiriso, kana mammalia cells kuti pagadzirwe maantibody.

Kuongorora uye Kusarudza
Maraibhurari emasoja ekudzivirira chirwere akavakwa anoongororwa uye anosarudzwa kuti aone masoja ekudzivirira chirwere ane hunhu hwaanoda achishandisa nzira dzekuongorora dzinoshandisa nzira dzekuongorora dzakanyanya. Izvi zvinogona kusanganisira nzira dzakadai sekuratidza mapoja, kuratidza yeast, kana kuratidza ribosome, zvichienderana nefomati yemaraibhurari uye mashandisirwo awo.

Basa rekuvaka maraibhurari eAntibody Akagadzirwa

Alpha Lifetech Inc.Inopa vatengi dhizaini imwe chete uye sevhisi yekugadzira maraibhurari ekudzivirira chirwere kuburikidza nekubatanidza ma repertoires eV-gene ane heavy uye light chain mu phage vector vachishandisa Cre-lox site-specific recombination system kugadzira repertoire yeFabs inoratidzwa pa phage ine 6.5 × 10^10 clones. Raibhurari iyi yakaburitsa Abs ichirwisa maantigen akawanda, mamwe ane nanomolar affinities. Kugadzirwa kweraibhurari yekudzivirira chirwere chemunhu kwakavakirwa paM13 phage display technology (inoratidzwa mumufananidzo uri pazasi).