Basa rekuvaka raibhurari yekuratidza mbiriso

Alpha Lifetech inonyanya kugadzira raibhurari yekuratidza yeast uye kuongorora maraibhurari ekudzivirira zvirwere, ichipa kugona kugadzira mhando dzakasiyana dzemaraibhurari ekudzivirira zvirwere uye kuaongorora kuti aone kana paine hukama hwakakura uye ma antibodies chaiwo kune vatengi pasi rese. Nechikwata chevaongorori vane hunyanzvi, tekinoroji yepamusoro uye michina, tinogona kuvaka maraibhurari ekudzivirira zvirwere tichitarisa mapuroteni akasiyana-siyana ane chekuita nezvirwere.

Mbiriso yekuratidza sisitimu yekumashure

Yeast display tekinoroji ye eukaryotic protein expression inoshandisa intracellular protein translocation mechanism (GPI anchor) ye yeast surface display system kudzivirira kufamba uye kuratidza exogenous antibodies kana ma protein genes pamusoro pe yeast. Yeast surface display system ine protein modification yakakwana mushure mekushandura uye secretion mechanism, iyo yakakodzera kupeta, kugadzirisa uye kutakurwa kwemapuroteni akagadzirwa nevanhu. Nokudaro, ma antibodies anogadzirwa ne yeast display system ari pedyo nema antibodies emuno evanhu muchimiro uye mashandiro, uye anopa mukana wekurapa zvirwere.

Sisitimu yekuratidza mbiriso inoita kuti pave nema antibodies chaiwo emapuroteni, mapeptide, mamorekuru madiki, uye maT-cell receptors. Ma antibodies aya ane zvakanakira zvekushanda zvakasiyana-siyana, kuyerwa uye kushanda zvakanaka kwekurapa. Kushandiswa kwetekinoroji yekuratidza mbiriso kwakatokura, iyo inogona kushandiswa kwete chete pakutsvaga mbiriso yekuratidza mbiriso, asiwo pakuongorora mapuroteni muraibhurari, kushanduka kwemapuroteni, kuvaka raibhurari yeantigen, kurongedza ma antibodies affinity, maturation ye antibody affinity, kugadzirwa kwevaccine, immuno-biocatalysis, uye biosensors, nezvimwewo, izvo zvine mukana wakakura muminda yekuongorora, kurapwa kwezvirwere uye tsvakiridzo yezvekurapa. Pamusoro pezvo, mapuroteni akagadzirwa kuburikidza ne antigenic mbiriso pamusoro pechiratidzo ari pedyo nechimiro chawo chechisikigo, zvichiita kuti ave akakodzera kuongororwa kwe serological senge ELISA. mbiriso inoratidza-Alpha Lifetech

Mufananidzo 1: Pfungwa yekuratidza mbiriso

Maitiro ekuvaka raibhurari yekuratidzira mbiriso

Maraibhurari edu embiriso ane kugona kukuru uye akasiyana-siyana, izvo zvinopa hwaro hwepamusoro hwekuongorora zvinobudirira maantibody sequences chaiwo. Tinogona kuvaka mhando dzakasiyana dzemaraibhurari embiriso (IgG, scFv, VHH, Fab antibody libraries), maraibhurari emapuroteni, maraibhurari epeptide, maraibhurari ecDNA, nezvimwewo zvichienderana nezvaunoda. Pamusoro pezvo, tinogonawo kugadzira maraibhurari embiriso ane hunhu hwakasiyana-siyana, kusanganisira maraibhurari ekudzivirira muviri, maraibhurari echisikigo, maraibhurari ekugadzira, maraibhurari esemi-synthetic, uye maraibhurari ezvirwere zvemaantibody. pYD ndiyo vector yedu inowanzoshandiswa yembiriso pamusoro pechiratidziro.

Tinokwanisa kuvaka maraibhurari embiriso ane simba reraibhurari rinosvika 10^9, ne100% insertion rate uye diversity. Tisati taendesa raibhurari yembiriso, tinosimbisa zvakasimba kuti tive nechokwadi chekusiyana, kuvimbika uye kushanda kwemajini eantibody. Kuti tive nechokwadi chekuti ma antibodies akaongororwa ane affinity yakanyanya uye specificity, tinoshandisa real-time quantitative evaluation ye antibody raibhurari. Fluorescence-activated cell sorting (FACS) inoongorora quantitatively kuti ione shanduko mu antibody expression uye activity, iyo inoshandisa zviratidzo zvakasiyana pa antibody ne antigen kuti ione simba rekubatanidza uye antibody expression pamusoro pemasero embiriso. Tinoshandisa FACS binding assays kuongorora quantitatively ma antibodies ane affinity yakakura uye selectivity. Kushanda zvakanaka kwe display ye yeast display nanobody library yedu neFACS kunopfuura 95%. Kazhinji, yeast experimentation inosanganisira rounds 3-5 dzekuongorora nekuwedzera, zvichitibvumira kuwana ma antibodies ane affinity yakawanda munguva pfupi.

Sisitimu yekuratidza mbiriso yave chishandiso chine simba mukushanduka-shanduka kwemapuroteni. Alpha Lifetech inopa masevhisi ekuongorora mapuroteni, kuchenesa, uye kinetic ekugadzirira maantibody emu vitro. Tine kugona kugadzirira mapuroteni makuru uye kuvandudza kuchena kwemapuroteni emu recombinant zvinobudirira. Isu tinopa sevhisi imwe chete kubva pakugadzira majini kusvika pakugadzirwa kwemapuroteni emu recombinant uye kuchenesa. Tichishandisa sisitimu yedu yepamusoro yekuratidza mbiriso, takabudirira kugadzirira ma antibodies ane picomolar (pM) affinity yepamusoro, inova budiriro huru mu tekinoroji yekugadzirira maantibody emu recombinant emu vitro.