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ALP64562-Recombinant Rhesus Macaque CD160 Borotiin, Fc Tag
Borotiin
ALP64562-Recombinant Rhesus Macaque CD160 Borotiin, Fc Tag
2.png

ALP64562-Recombinant Rhesus Macaque CD160 Borotiin, Fc Tag

  • Lambarka buugga: ALP64562
  • Bartilmaameed CD160
  • Noocyada martida loo yahay: Rhesus Macaque
  • Daahirnimo: >90%-95% sida uu go'aamiyay SDS-PAGE
  • Nidaamka Muujinta: Unugyada HEK293

Borotiinka Rhesus Macaque CD160 ee dib loo habeeyay, Fc Tag waxaa lagu soo saaraa Unugyada HEK293, hidda-wadaha bartilmaameedka ah ee ku jira Met1-Leu158 waxaa lagu muujiyaa calaamadda Fc ee C-terminus. Borotiinka Rhesus Macaque CD160 ee dib loo habeeyay, wuxuu tilmaamayaa qaab hidde ahaan loo sameeyay oo ah borotiinka CD160 ee ka soo jeeda noocyada Rhesus Macaque, kaas oo wax laga beddelay iyadoo lagu daray calaamadda Fc. Calaamadda Fc waxaa laga soo qaatay molecule immunoglobulin G (IgG) waxayna u oggolaanaysaa nadiifinta iyo ogaanshaha borotiinka CD160 si fudud.

Alpha Lifetechwaxay ku faantaa inay bixiso badeeco borotiin dib loo habeeyay,Borotiin Rhesus Macaque CD160 oo dib loo habeeyay, si loo daboolo baahiyaha macaamiisha. Borotiinka CD160 ee dib loo isku daray oo leh calaamadda Fc waxaa loo isticmaali karaa codsiyo cilmi baaris oo kala duwan, oo ay ku jiraan barashada shaqada iyo isdhexgalka CD160 ee unugyada difaaca jirka, baaritaanka doorkiisa ku leh jawaabaha difaaca jirka iyo cudurrada, iyo sidii xakameyn ama heer caadi ah oo ku saabsan daraasadaha is-dhexgalka borotiinka-borotiinka. DooroAlpha LifetechBadeecada borotiinka dib-u-habaynta ah ee loogu talagalay xal daaweyn oo la isku halleyn karo, wax ku ool ah, iyo kharash-ool ah.

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ALP64562-Recombinant Rhesus Macaque CD160 Borotiin, Fc Tag

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01
adeegga lagu taliyay
xiriiro degdeg ah
Nala soo xiriir
Taariikhda Badeecada Qeexitaanka BadeecadaSu'aalaha Badiya La Weydiiyo

Taariikhda Badeecada

Antigen-ka CD160 waa borotiin xuubka unugyada ah oo ka kooban hal domain oo Ig-likeV-nooc (immunoglobulin-la mid ah). CD160 waa soo-dhoweeye dusha sare ee lymphocyte-ka GPI-anchored kaas oo muujinta badanaa ku kooban tahay qaybta dhiigga ee aadka u sunta ah ee CD56(dim) CD16(+) ee NK. CD160 waa soo-dhoweeye muujinaya gaar ahaansho ballaaran oo loogu talagalay molecules-ka fasalka I ee caadiga ah iyo kuwa aan caadiga ahayn ee MHC. CD160 waxaa lagu muujiyaa beeryarada, dhiigga durugsan, iyo lintestine-ka yar. Muujinta CD160 waxay ku kooban tahay lymphocytes-ka NK iyo T ee shaqeynaya. CD160 waxay u dhaqantaa sidii soo-dhoweeye isku-dhafan oo loogu talagalay faafitaanka CD3 ee unugyada T ee CD4+ CD160+ T ee laga soocay nabarrada maqaarka ee bararka leh. Lymphocytes-ka NK ee firfircoon waxay sii daayaan qaab milmi kara oo CD160 ah kaas oo si firfircoon u wiiqaya jawaab celinta cytotoxic-ka CD8(+) T ee MHC-I-gaarka ah.

Qeexitaanka Badeecada

Lambarka Kataloogga

ALP64562

Magaca Badeecada

Borotiin Rhesus Macaque CD160 oo dib loo habeeyay, Fc Tag

Erayo isku mid ah

CD160, borotiin CD160, borotiin Rhesus Macaque CD160, Borotiin Rhesus Macaque CD160 oo dib loo habeeyay, borotiin CD160 oo ka tirsan unugyada difaaca jirka, antibody-ga CD160, antigen-ka CD160

Magaca loo yaqaan

Cudurka 'antigen'-ka ee CD160, CD160

Astaanta rasmiga ah

CD160

Noocyada

Rhesus Macaque

Martigeliyaha muujinta

Unugyada HEK293

Qoyska Borotiinnada

Qoyska Sare ee Immunoglobulin

Cabbirka

50g, 100g

Nadiifinta

Nadiifinta Xiriir

Daahirnimo

>90%-95% sida uu go'aamiyay SDS-PAGE

Endotoxin

Kaydka

Waxaa lagu bixiyaa PBS, pH 7.4

Kaydinta

Kaydinta muddada dheer, ku kaydi sheyga -80°C. Ka fogow wareegyada barafaynta iyo dhalaalinta ee soo noqnoqda.

Xaaladda Maraakiibta

Budada Lyophilized waxaa lagu soo raraa heerkulka deegaanka. Qaab dareere ah ayaa lagu soo raraa baraf qalalan.

Fiiro gaar ah

Badeecadan waxaa loogu talagalay cilmi-baaris oo keliya. Badeecadan looma isticmaali karo aadanaha.

Su'aalaha Badiya La Weydiiyo

  • + -

    Waa maxay koromatografiga xiriirka ka dhexeeya iyo sidee loo isticmaalaa nadiifinta borotiinka?

    Koromatografiga Affinity wuxuu adeegsadaa isku xidhka gaarka ah ee u dhexeeya borotiinka bartilmaameedka ah iyo ligand-ka aan dhaqdhaqaaq lahayn ee ku jira shaxda koromatografiga. Borotiinka bartilmaameedka ah wuxuu si xushmad leh ugu xidhmaa shaxda, taasoo u oggolaanaysa nadiifinta si aad u gaar ah. Calaamadaha isku xidhka caadiga ah waxaa ka mid ah calaamadaha histidine, calaamadaha antibody-ga, iyo ligands-ka gaarka u ah borotiinka.

  • + -

    Waa maxay koromagrafiga is-weydaarsiga ion sideese loogu isticmaalaa nadiifinta borotiinka?

    Koromagrafiga is-weydaarsiga ion-ku wuxuu kala saaraa borotiinnada iyadoo lagu saleynayo dallacaadda saafiga ah ee pH gaar ah. Borotiinnada leh dallacaadda saafiga ah waxay ku xidhmaan reinsiyada is-weydaarsiga ion-ka ee taban (chromatography-ga is-weydaarsiga anion), halka borotiinnada leh dallacaadda taban ee saafiga ah ay ku xidhmaan reinsiyada togan ee togan (chromatography-ga is-weydaarsiga cation-ka).

  • + -

    Waa maxay chromatography-ga ka-saarista cabbirka iyo sidee loogu isticmaalaa nadiifinta borotiinka?

    Koromagrafiga ka-saarista cabbirka wuxuu kala soocaa borotiinnada iyadoo lagu saleynayo cabbirkooda iyo qaabkooda. Borotiinnada waaweyn ayaa marka hore ka soo baxa tiirka koromagrafiga, halka borotiinnada yaryar laga saaro daloollada marxaladda taagan. Habkani waxaa badanaa loo isticmaalaa nadiifinta milixda iyo is-weydaarsiga kaydka iyo sidoo kale nadiifinta isku-dhafka borotiinka.

  • + -

    Sidee loo qiimeeyaa daahirnimada iyo wax-soo-saarka inta lagu jiro nadiifinta borotiinka?

    Nadiifnimada iyo wax-soo-saarka waxaa badanaa lagu qiimeeyaa iyadoo la adeegsanayo farsamooyin falanqayn ah sida SDS-PAGE, western blotting, UV absorbance spectroscopy, iyo Bradford ama BCA protein protein ...

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