Madal Tiknoolajiyadda Hybridoma
Alpha Lifetech waxay macaamiisha siisaa adeegyo tayo leh oo la hubo oo ku saabsan antibody-ga iyo antibody-ga dib-u-habaynta ah.
Codso Qiimayn01
Madal Tiknoolajiyadda Hybridoma
Alpha Lifetech waxay leedahay waayo-aragnimo qani ah oo ku saabsan horumarinta antibody-ga. Sannado badan, Alpha Lifetech waxay aasaastay goob dhammaystiran oo lagu horumarinayo antibody-ga (oo ay ku jiraan horumarinta antibody-ga monoclonal, horumarinta antibody-ga polyclonal, horumarinta antibody-ga monoclonal-ka ee dib-u-habaynta, iyo adeegyada kale ee diyaarinta antibody-ga iyo horumarinta).
Alpha Lifetech waxay macaamiisha siisaa adeegyo iyo adeegyo tayo leh oo la hubo oo ku saabsan antibody-ga iyo antibody-ga dib-u-habaynta, antibody-yada la diyaariyeyna waxay leeyihiin qeexitaan xooggan iyo xasillooni wanaagsan. Qalab iyo qalab badan oo lagu nadiifiyo antibody-ga ayaa loo isticmaali karaa in lagu bixiyo adeegyo nadiifinta antibody-ga oo laga helo ilo kala duwan sida bakaylaha, idaha, digaagga, unugyada difaaca jirka ee monoclonal-ka jiirka, iyo sidoo kale adeegyada nadiifinta protein-ka ee A/G iyo adeegyada kala-soocidda antibody-ga.
Iyada oo ku saleysan farsamooyin kala duwan oo loogu talagalay diyaarinta antibody-ga sida teknoolojiyadda unugyada hybridoma, Alpha Lifetech waxay bixin kartaa adeegyo gaar ah oo loogu talagalay monoclonal iyo polyclonal antibody-yada noocyada kala duwan. Intaa waxaa dheer, adeegyo kala duwan laga bilaabo taxanaha sare ee wax soo saarka, diyaarinta antibody-ga, nadiifinta antibody-ga ilaa xaqiijinta antibody-ga ayaa sidoo kale la bixin karaa. Waxaan dooran doonnaa habka nadiifinta iyadoo lagu saleynayo ujeeddada gaarka ah ee macaamiisha. Iyada oo lagu saleynayo nidaamyada dhammaystiran ee madal sida madal horumarinta antibody-ga iyo madal borotiin ah, Alpha Lifetech waxay daboolaysaa adeegyada kor iyo hoos ee wax soo saarka antibody-ga, bixinta nadiifinta antibody-ga, taxanaha sare ee wax soo saarka antibody-ga, xaqiijinta antibody-ga iyo adeegyada kale ee farsamada.
Tiknoolajiyadda Hybridoma
Tiknoolajiyadda Hybridoma waxaa loo isticmaali karaa in lagu diyaariyo unugyada difaaca jirka ee monoclonal. Iyadoo la isku darayo unugyada beeryarada ee jiirarka la tallaalay iyo unugyada myeloma ee aadanaha ama jiirarka, unugyada hybridoma ayaa la sameeyaa waxayna soo saaraan unugyada difaaca jirka ee gaarka ah. Unugyada difaaca jirka ee monoclonal ee laga soo saaro jiirka ayaa loo isticmaali karaa cilmi-baaris cilmiyeed sida baaritaanka immunosorbent-linked enzyme-linked, radioimmunoassay, immunohistochemistry, flow cytometry, nadiifinta borotiinka, iwm. Muunadaha antibody-ga ee monoclonal waxaa ka mid ah ascites, hybridoma supernatant, iwm. Muunadda laga helo duritaanka unugyada hybridoma godka caloosha ee xayawaanka iyo isticmaalka deegaanka jireed ee godka caloosha ee xayawaanka si loo soo saaro unugyada difaaca jirka waxaa loo yaqaan ascites sample. Unugyada Hybridoma ee lagu duro godka caloosha ee xayawaanka ayaa ku badin kara oo soo saari kara unugyada difaaca jirka ee monoclonal, kuwaas oo ku ururi kara ascites. Sababtoo ah suurtagalnimada wasakhda (borotiinnada xayawaanka, hoormoonnada, iwm.) ee unugyada difaaca jirka, waa la nadiifiyaa. Iyada oo ku saleysan shaqada dheecaanka ee lymphocytes-ka B, unugyada difaaca jirka ee monoclonal badanaa waxaa lagu soo daayaa bartamaha dhaqanka marka la beerayo unugyada hybridoma. Dareeraha ka kooban unugyada difaaca jirka ee monoclonal ee ku jira habka dhaqanka waxaa loo yaqaan hybridoma supernatant. Nadiifnimada unugyada difaaca jirka ee monoclonal ee ku jira dheecaanka sare waa mid aad u sareysa, laakiin nadiifin dheeraad ah ayaa loo baahan yahay si loogu diyaariyo baaxad weyn maadaama laga helo dhaqanka in vitro.
Nadiifinta unugyada difaaca jirka waxay ka sooci kartaa unugyada difaaca jirka ee nadiifka sare leh isku-dhafka. Waqtigan xaadirka ah, nadiifinta unugyada difaaca jirka iyo kala-soocidda iyo nadiifinta unugyada difaaca jirka ayaa ah hababka nadiifinta unugyada difaaca jirka ee si caadi ah loo isticmaalo, oo ay ugu badan yihiin nadiifinta borotiinka A/G, chromatography-ga is-weydaarsiga ion, shaandhaynta jel, roobabka iyo chromatography-ga is-dhexgalka hydrophobic. Kuwaas waxaa ka mid ah, adeegga nadiifinta borotiinka A/G ee firfircoonida ayaa si weyn looga jecel yahay codsiyada kala duwan ee nadiifinta unugyada difaaca jirka. Habka Alpha Lifetech u diyaarinayso unugyada difaaca jirka ee monoclonal oo ku salaysan tignoolajiyada hybridoma waxaa lagu muujiyay Jaantuska 1.
Jaantuska 1aad Jaantuska habka loo diyaariyo unugyada difaaca jirka ee monoclonal oo ku salaysan tiknoolajiyada hybridoma.
Tiknoolajiyada Hybridoma iyo Tiknoolajiyada Ka-hortagga Cudurka Ka-hortagga
Isku-xidhka daawada ka hortagga jirka (ADC) waa wakiil bayooloji cusub oo kiimiko ahaan isku xidha unugyada difaaca jirka iyo daawooyinka sunta ah. Isku-xidhka daawada ka hortagga jirka wuxuu isku daraa awoodda aqoonsiga gaarka ah ee unugyada difaaca jirka iyo saameynta dilka xooggan ee daawooyinka sunta ah, wuxuuna si sax ah u gaarsiiyaa daawooyinka sunta ah unugyada burooyinka iyada oo loo marayo unugyada difaaca jirka, taasoo keenta daaweyn sax ah. Qaybta antibody-ga ee daawooyinka ADC waxaa badanaa lagu diyaariyaa tiknoolajiyada hybridoma ama farsamooyinka kale ee wax soo saarka antibody-ga, sida tiknoolajiyada antibody-ga injineernimada hidde-sidaha. Tiknoolajiyadda Hybridoma waxay bixisaa walxo tayo sare leh oo antibody ah oo loogu talagalay daawooyinka ADC. Daawooyinka ADC iyo antibody-ga monoclonal ee lagu diyaariyey tiknoolajiyadda hybridoma waxay door muhiim ah ka ciyaaraan meelaha sida daaweynta kansarka. Waxaa loo isticmaali karaa kaligood ama isku darka si loo xoojiyo waxtarka daaweynta.
Unugyada difaaca jirka ee monoclonal-ka oo ay diyaarisay tiknoolajiyadda hybridoma waxay leeyihiin gaar ahaansho sare, waxay si sax ah u xidhi karaan oo u aqoonsan karaan antigens gaar ah, waxaana lagu soo saari karaa heer aad u ballaaran. Iyada oo loo marayo tiknoolajiyada isku xigxiga hybridoma, hiddo-sideyaasha antibody-ga ee unugyada hybridoma ayaa la soo saari karaa oo la kala saari karaa si loo helo taxane u dhigma, taas oo faa'iido u leh muujinta antibody-ga ee xigta. Unugyada difaaca jirka ee monoclonal-ka ee lagu diyaariyey tiknoolajiyada hybridoma waxaa loo isticmaali karaa in lagu ogaado calaamadaha cudurrada gaarka ah, sida calaamadaha burooyinka, antigens-ka fayraska, iwm., iyagoo siinaya taageero xooggan oo loogu talagalay ogaanshaha hore ee cudurrada. Intaa waxaa dheer, waxaa sidoo kale loo isticmaali karaa tijaabooyinka sida immunoblotting iyo immunohistochemistry si loo barto muujinta borotiinka, deegaanka, qaab-dhismeedka, iyo shaqada.
tiknoolajiyada hybridoma Soo saarista Adeegga Ka-hortagga Jirka Hab-shaqo
| Tallaabooyinka | Macluumaadka Adeegga | Jadwalka |
|---|---|---|
| Tallaabada 1aad: Tallaalka xoolaha | (1) Tallaalka xoolaha 4 jeer, tallaalka xoojiyaha 1 qiyaas, wadarta guud ee 5 qiyaasood oo la tallaalay. (2) Serum taban ka hor inta aan la qaadin tallaalka, ELISA-na waxaa lagu sameeyay qiyaasta afraad si loo ogaado titer-ka serum-ka. (3) Haddii heerka difaaca jirka ee serum-ka ee qiyaasta afraad uu buuxiyo shuruudaha, hal qiyaas oo tallaal ah oo dheeraad ah ayaa la siin doonaa 7 maalmood ka hor ururinta dhiigga. Haddii uusan buuxin shuruudaha, tallaalka joogtada ah wuu sii socon doonaa. (4) Awood aqoon leh, Unugyada Spleen-ka ayaa la go'doomiyaa si loogu daro unugyada. | 10-12 toddobaad |
| Tallaabada 2: Isku-darka iyo Shaandhaynta | (1) Isku-darka: Ka dib tallaalka, unugyada beeryarada jiirka ayaa la soo ururiyay oo lagu daray unugyada myeloma. (2) Fiditaanka iyo baaritaanka: Unugyada hybridoma ee la helay waxaa lagu sameeyay faafitaan iyo baaritaan ELISA si loo helo tirooyin aad waxtar u leh. | 6 toddobaad |
| Tallaabada 3: Subclone | Ku-samaynta 15-25 unug oo hooyooyin ah oo togan iyadoo la adeegsanayo habka kala-soocidda xaddidan, ugu dambayntiina go'aaminta 10-15 unugyo togan | 3-4 toddobaad |
| Tallaabada 4: Soo saarista Ascites iyo Nadiifinta Antibody-ga | (1) Soo saarista Ascites: 10-15 nooc oo hybridoma ah ayaa loo isticmaalaa soo saarista ascites ee jiirka (haddii tiro badan oo nooc oo hybridoma ah la helo, waxaa loo diri doonaa qaab unug) (2) Aqoonsiga Ascites: Ogaanshaha Ascites ELISA, awoodda (borotiin/fayras>10 ^ 5, molecule yar/peptide>10 ^ 4) si loo nadiifiyo hoos (3) Nadiifinta Borotiin A/G: Nadiifinta Ascites | 3-4 toddobaad |
| Tallaabada 5: Gaarsiinta | (1) Bixi 3-5 nooc oo ah unugyada difaaca jirka ee monoclonal hybridoma. (2) Unug kasta oo hybridoma ah wuxuu bixiyaa 1mg oo ah antibody la safeeyey (ascites cleaned). (3) Warbixinta Habka Tijaabada ah | 1 toddobaad |
Noocyo Badan
Difaaca waxaa la siin karaa xayawaanno kala duwan, oo ay ku jiraan laakiin aan ku xaddidnayn jiirka, bakaylaha, idaha, iwm.
Bartilmaameedyo Badan
Iyada oo ku saleysan tignoolajiyada hybridoma, adeegyo badan oo lagu ogaanayo unugyada difaaca jirka ayaa la heli karaa: borotiinno, peptides, molecules yar yar, fayrasyo, borotiinno xuubka, mRNA, iwm.
Diiwaanada Raadinta
Heerarka xakamaynta tayada QC (awoodda difaaca jirka, xakamaynta tayada PBMC, xakamaynta tayada maktabadda, iyo xakamaynta tayada xaqiijinta baaritaanka), warbixinnada tijaabada Ingiriisiga, diiwaannada tijaabada asalka ah
Barnaamij Shakhsiyeysan
Habaynta barnaamij shaqsiyeed oo mid-mid ah si loo daboolo baahiyaha mashruuca cilmi-baarista ee macaamiisha kala duwan
Adeegga Horumarinta Ka-hortagga Jirka ee Monoclonal
Waxaan bixin karnaa adeegyo tayo sare leh, saafinimo sare leh, iyo horumarin antibody monoclonal ah oo gaar ah, oo ay ku jiraan soo saarista antibody monoclonal jiirka iyo antibody monoclonal bakayle
Madal Tiknoolajiyadda Hybridoma
Oo ay ku jiraan barnaamijka tallaalka, adeegyada diyaarinta antibody-ga, nadiifinta antibody-ga, isku xigxiga sare ee antibody-ga, xaqiijinta antibody-ga, iwm.
Madal Kala-soocidda Unugyada Hal-B ah
Alpha Lifetech waxay leedahay faa'iidooyin ku saabsan waqtiga baaritaanka iyo helitaanka unugyada difaaca jirka ee tayo sare leh. Waxay bixin kartaa naqshadaynta antigen-ka, sameynta, iyo wax ka beddelka, difaaca xayawaanka, baaritaanka kobcinta unugyada B ee hal-hal ah, iyo isku xigxiga unugyada hal-hal ah.
Madal Horumarinta Ka-hortagga Jirka ee Muuqaalka Muuqaalka (Phage Display Antibody)
Alpha Lifetech waxay bixin kartaa adeegyo farsamo oo horumarinta antibody-ga bandhigga phage-ka laga bilaabo diyaarinta antibody-ga, nadiifinta antibody-ga, taxanaha antibody-ga, iwm.
Kheyraadka Tiknoolajiyada La Xiriira
Hordhac Tiknoolajiyadda HybridomaFIIDIYOOW
Haddii aad qabtid wax su'aalo ah, fadlan xor u noqo inaad nala soo xiriirto wakhti kasta.
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2018-07-16 
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