Madal Horumarinta Ka-hortagga Jirka ee Muuqaalka Muuqaalka (Phage Display Antibody Platform)
Iyada oo ku saleysan dhismaha nidaamka madal dhamaystiran ee madal antibody ah, Alpha Lifetech waxay bixin kartaa adeegyo farsamo oo ka socda diyaarinta antibody-ga, nadiifinta antibody-ga, taxanaha antibody-ga, iwm.
NALA SOO XIRIIR01
Madal Horumarinta Ka-hortagga Jirka ee Muuqaalka Muuqaalka (Phage Display Antibody Platform)
Alpha Lifetech, oo leh khibrad ballaaran oo mashruuceeda ah oo ku saabsan daahfurka antibody-ga, waxay bixin kartaa adeegyo antibody monoclonal iyo polyclonal ah oo loogu talagalay noocyo badan, iyo sidoo kale dhismaha maktabadda antibody-ga iyo adeegyada baaritaanka. Alpha Lifetech waxay macaamiisha siin kartaa alaabada antibody-ga ee la hubo oo tayo leh iyo antibody-ga dib-u-habaynta, iyo sidoo kale adeegyo u dhigma, si loo diyaariyo antibody-ga hufan, aad u gaar ah, iyo xasilloon. Annagoo adeegsanayna aaladaha antibody-ga ee dhammaystiran iyo injineernimada antibody-ga, waxaan bixinnaa adeegyo daboolaya kor iyo hoosba wax soo saarka antibody-ga, oo ay ku jiraan adeegyada farsamada sida diyaarinta antibody-ga, nadiifinta, taxanaha antibody-ga, iyo xaqiijinta.
Alpha Lifetech waxay leedahay goob lagu ogaanayo unugyada difaaca jirka ee bisil kaas oo macaamiisha siiya adeegyo kala duwan oo ay ku jiraan difaaca xayawaanka, dhismaha iyo baaritaanka maktabadda unugyada difaaca jirka ee phage, taxanaha unugyada difaaca jirka, muujinta unugyada difaaca jirka, nadiifinta unugyada difaaca jirka, xaqiijinta unugyada difaaca jirka, iyo calaamadaynta unugyada difaaca jirka ee ku salaysan tiknoolajiyada hybridoma, tignoolajiyada unugyada B ee hal-halka ah, tignoolajiyada muujinta unugyada difaaca jirka, iyo waxyaabo kaloo badan. Alpha Lifetech waxay leedahay khad wax soo saar oo caadi ah, marka lagu daro adeegyada diyaarinta unugyada difaaca jirka, adeegyada taageerada sida bini'aadamnimada unugyada difaaca jirka, bislaanshaha unugyada difaaca jirka, naqshadeynta iyo horumarinta daawada ADC, iyo naqshadeynta taxanaha xiga ee CAR-T ayaa sidoo kale la bixin karaa. Isla mar ahaantaana, Alpha Lifetech waxay dhistay maktabad unugyada difaaca jirka ah oo ku salaysan goobaha tignoolajiyada ee M13, T4, iyo T7, oo leh awood kaydin ilaa 10^8-10^9. Heerka togan, heerka gelinta, iyo kala duwanaanshaha maktabadda dhammaantood waxay gaari karaan in ka badan 90%.
Tiknoolajiyada bandhigga wejiga
Soo saarista unugyada difaaca jirka ee monoclonal waxaa markii hore la diyaariyey iyadoo la adeegsanayo tignoolajiyada unugyada difaaca jirka ee monoclonal hybridoma si loo soo saaro unugyada difaaca jirka ee monoclonal hybridoma jiirka. Gaar ahaan, iyadoo la isku darayo unugyada beeryarada ee jiirarka la tallaalay oo leh unugyada myeloma ee aadanaha ama jiirka, unugyada hybridoma ayaa la sameeyaa, kuwaas oo soo saara unugyada difaaca jirka ee monoclonal hybridoma gaar ah. Iyada oo la adeegsanayo wax ka beddelka aadanaha ee unugyada difaaca jirka ee monoclonal jiirka iyo wax ka beddelka injineernimada hidde-sidaha ee unugyada difaaca jirka si loo siiyo gobol joogto ah oo immunoglobulin ah oo aadanaha ah, difaacooda waa la dhimi karaa. Injineernimada unugyada difaaca jirka ee monoclonal waxaa si weyn loogu isticmaalaa codsiyada biomedical iyo kiliinikada.
Alpha Lifetech waxay bixin kartaa adeegyo hal-joojin ah oo injineerinka antibody-ga ah laga bilaabo naqshadeynta hab-raaca cilmi-baarista kahor-kiliinikada ilaa daahfurka daawooyinka antibody-ga (ADCs) iyo xaqiijinta xayawaanka. Isku-xidhka daawooyinka antibody-ga (ADCs) waxay keeni karaan daawooyinka kiimoterabiga unugyada kansarka. Ka dib marka ay ku xidhmaan bartilmaameedyo gaar ah oo lagu muujiyo unugyada kansarka, ADC waxay sii deysaa daawooyinka cytotoxic-ka unugyada kansarka. Alpha Lifetech waxay sidoo kale siin kartaa macaamiisha adeegyo dhammaystiran oo ku saabsan bislaanshaha antibody-ga. Iyada oo la adeegsanayo hagaajinta isbeddelka horumarsan iyo tignoolajiyada baaritaanka phage-ka ee heerka sare ah, unugyada difaaca jirka ee leh xiriir gaar ah ayaa marka hore la baaraa, ka dibna isbeddellada amino acid-ka ayaa la soo bandhigaa si loo soo saaro noocyo kala duwan. Ka dib, unugyada difaaca jirka ee heerka sare ah ayaa la qiimeeyay oo la baaray iyadoo la adeegsanayo farsamooyinka baaritaanka. Ka dib wareegyo badan oo hagaajin iyo falanqayn qaab-dhismeed ah, unugyada difaaca jirka ee leh xiriir sare iyo gaar ahaansho xooggan ayaa ugu dambeyntii la helay.
Alpha Lifetech waxay dhisi kartaa noocyo kala duwan oo maktabado bandhig ah oo ah phage-ka difaaca jirka, oo ay ku jiraan maktabadaha difaaca jirka, maktabadaha asalka ah, maktabadaha nus-sameysan, iyo maktabadaha synthetic-ga ah. Iyada oo ku saleysan awoodda sare ee maktabadaha antibody-ga, antibody-yada aadka u gaarka ah ayaa la heli karaa. Vectors-yo badan oo phagemid ah sida pMECS, pComb3X, iyo pCANTAB 5E ayaa la bixin karaa, iyo sidoo kale noocyada sida TG1 E. coli, XL1 Blue, iyo ER2738. Marka lagu daro awoodda maktabadda oo ah ilaa 10^9, heerka gelinta jajabka bartilmaameedka ee maktabadda ayaa sidoo kale sarreeya, taasoo abuuraysa shuruudo ku filan oo lagu baarayo antibody-yada qancinaya macaamiisha. Shaxda socodka adeegga diyaarinta antibody-ga ee Alpha Lifetech oo ku salaysan tiknoolajiyada bandhigga phage waxaa lagu muujiyay Jaantuska 1.
Jaantuska 1aad Jaantuska habka loo diyaariyo unugyada difaaca jirka ee monoclonal iyadoo lagu saleynayo tiknoolajiyada bandhigga phage.
Habka Shaqada Soo-saarka Ka-hortagga Jirka ee Muuqaalka Wajiga (Phage Display Antibody)
| Tallaabooyinka | Macluumaadka Adeegga | Jadwalka |
|---|---|---|
| Tallaabada 1aad: Tallaalka xoolaha | (1) Tallaalka xoolaha 4 jeer, tallaalka xoojiyaha 1 qiyaas, wadarta guud ee 5 qiyaasood oo la tallaalay. (2) Serum taban ka hor inta aan la qaadin tallaalka, ELISA-na waxaa lagu sameeyay qiyaasta afraad si loo ogaado titer-ka serum-ka. (3) Haddii heerka difaaca jirka ee serum-ka ee qiyaasta afraad uu buuxiyo shuruudaha, hal qiyaas oo tallaal ah oo dheeraad ah ayaa la siin doonaa 7 maalmood ka hor ururinta dhiigga. Haddii uusan buuxin shuruudaha, tallaalka joogtada ah wuu sii socon doonaa. (4) Awood u qalanta, ururinta dhiigga iyo kala soocida unugyada dhiigga cas | 10 toddobaad |
| Tallaabada 2: Diyaarinta cDNA | (1) Soo saarista RNA-ga PBMC Wadarta Guud (Qalabka Soo saarista RNA) (2) Diyaarinta RT-PCR ee daacadnimada sare leh ee cDNA (qalabka qoraalka ee dib loo rogi karo) | 1 maalin |
| Tallaabada 3: Dhismaha Maktabadda Ka-hortagga Jirka | (1) Iyadoo la adeegsanayo cDNA qaab ahaan, hiddo-sidayaasha waxaa lagu kordhiyay laba wareeg oo PCR ah. (2) Dhismaha iyo isbeddelka wejiga: isku-xidhka hidda-wadaha ee vector-ka phagemid, isbeddelka elektroporation ee bakteeriyada martida loo yahay ee TG1, dhismaha maktabadda antibody-ga. (3) Aqoonsi: Si aan kala sooc lahayn u dooro 24 nooc oo kala duwan, heerka togan ee aqoonsiga PCR + heerka gelinta. (4) Diyaarinta fajiyada ee caawinta leh: M13 kor u qaadida fajiyada + nadiifinta. (5) Badbaadinta maktabadda bandhigga wejiga | 3-4 toddobaad |
| Tallaabada 4: Baaritaanka Maktabadda Ka-hortagga Jirka (3 wareeg) | (1) Baaritaanka 3-wareeg ee caadiga ah (baaritaanka wejiga adag): baaritaanka cadaadiska si looga saaro unugyada difaaca jirka ee aan gaarka ahayn ilaa inta ugu badan ee suurtogalka ah. (2) Hal shay oo kor u qaadis ah oo loo sameeyay bakteeriyada oo la doortay + muujinta IPTG ee la soo saaray + ogaanshaha ELISA ee unugyada togan. (3) Dhammaan noocyada togan waxaa loo xushay taxanaha hidda-wadaha. | 4-5 toddobaad |
Adeegyada Taageerada
Waxaan bixin karnaa adeegyo dhisme maktabad difaac oo ku salaysan xayawaan iyo adeegyo baaritaan oo maktabadda difaaca jirka dabiiciga ah iyadoo loo eegayo baahiyaha macaamiisha
Bartilmaameedyo Badan
Adeegyada ogaanshaha antibody-ga ee bartilmaameedka badan ayaa la heli karaa: borotiinno, peptides, molecules yaryar, fayrasyo, borotiinno xuubka, mRNA, iwm.
Vektorrada Badan
Adeegga dhismaha maktabadda shaqsiyeed, waxaan bixin karnaa noocyo kala duwan oo bacteriophage ah oo ay ku jiraan PMECS, pComb3X, iyo pCANTAB 5E, waxaanan wax ka beddeli karnaa iyadoo loo eegayo baahiyaha macaamiisha.
Madal Qaangaar ah
Awoodda kaydinta waxay gaari kartaa 10 ^ 8-10 ^ 9, heerarka gelinta dhammaantood waxay ka sarreeyaan 90%, isku xirnaanta unugyada difaaca jirka ee laga helo baaritaanka guud ahaan waa heerka nM pM.
Adeegga Horumarinta Ka-hortagga Jirka ee Monoclonal
Waxaan bixin karnaa adeegyo tayo sare leh, saafinimo sare leh, iyo horumarin antibody monoclonal ah oo gaar ah, oo ay ku jiraan soo saarista antibody monoclonal jiirka iyo antibody monoclonal bakayle
Madal Tiknoolajiyadda Hybridoma
Oo ay ku jiraan barnaamijka tallaalka, adeegyada diyaarinta antibody-ga, nadiifinta antibody-ga, isku xigxiga sare ee antibody-ga, xaqiijinta antibody-ga, iwm.
Madal Kala-soocidda Unugyada Hal-B ah
Alpha Lifetech waxay leedahay faa'iidooyin ku saabsan waqtiga baaritaanka iyo helitaanka unugyada difaaca jirka ee tayo sare leh. Waxay bixin kartaa naqshadaynta antigen-ka, sameynta, iyo wax ka beddelka, difaaca xayawaanka, baaritaanka kobcinta unugyada B ee hal-hal ah, iyo isku xigxiga unugyada hal-hal ah.
Madal Horumarinta Ka-hortagga Jirka ee Muuqaalka Muuqaalka (Phage Display Antibody Platform)
Alpha Lifetech waxay bixin kartaa adeegyo farsamo oo horumarinta antibody-ga bandhigga phage-ka laga bilaabo diyaarinta antibody-ga, nadiifinta antibody-ga, taxanaha antibody-ga, iwm.
Haddii aad qabtid wax su'aalo ah, fadlan xor u noqo inaad nala soo xiriirto wakhti kasta.
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2018-07-16 
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