Bandhiga Wajiga Horumarinta Kahortagga Jirka
Iyada oo ku saleysan dhismaha nidaamka madal dhamaystiran ee madal antibody, Alpha Lifetech ku siin kartaa adeegyo farsamo diyaarinta antibody, nadiifinta antibody, isku xigxiga antibody, iwm.
NALA SOO XIRIIR01
Bandhiga Wajiga Horumarinta Kahortagga Jirka
Alpha Lifetech, oo leh waayo-aragnimadeeda mashruuc ee ballaadhan ee sahaminta antibody-ga, waxay bixin kartaa adeegyada ka hortagga unugyada monoclonal iyo polyclonal ee noocyada badan, iyo sidoo kale dhismaha maktabadda kahortagga iyo adeegyada baaritaanka. Alpha Lifetech waxay siin kartaa macaamiisha tayada difaaca jirka ee tayada leh iyo dib-u-habaynta ka hortagga unugyada, iyo sidoo kale adeegyada u dhigma, si loo diyaariyo unugyada difaaca ee hufan, aadka u gaarka ah, iyo xasilloon. Anagoo adeegsanayna aaladaha lidka-iska-hortagga ee dhammaystiran iyo injineernimada antibody-ka, waxaan bixinaa adeegyo daboolaya kor iyo hoos-u-socodka wax-soo-saarka ka-hortagga, oo ay ku jiraan adeegyada farsamada sida diyaarinta-anti-body-ga, sifaynta, isku-xigta-ka-hortagga, iyo ansixinta.
Alpha Lifetech waxay leedahay madal daahfurka difaaca jirka oo qaan-gaar ah oo siisa macaamiisha adeegyo kala duwan oo ay ku jiraan xasaanadda xayawaanka, bandhiga bandhiga maktabadda lidka ku ah dhismaha iyo baarista, isku xigxiga antibody-ka, muujinta antibody-ka, nadiifinta antibody-ka, ansaxinta antibody-ka, iyo calaamadaynta antibody ee ku saleysan tignoolajiyada hybridoma, tignoolajiyada unugyada B ee hal-abuurka ah, tignoolajiyada bandhiga phage, iyo in ka badan. Alpha Lifetech waxay leedahay khad wax soo saar oo caadi ah, marka lagu daro adeegyada diyaarinta antibody-ka, adeegyada taageerada sida bini'aadantinimada antibody-ka, korriinka xidhidhnimada antibody, naqshadaynta iyo horumarinta daroogada ADC, iyo CAR-T naqshadaynta xigta ee soo socota ayaa sidoo kale la bixin karaa. Isla mar ahaantaana, Alpha Lifetech waxay dhistay maktabad ka hortag ah oo ku salaysan M13, T4, iyo T7 phage dhufto ee tignoolajiyada, oo leh awood kaydinta ilaa 10^8-10^9. Heerka togan, heerka gelinta, iyo kala duwanaanta maktabadda waxay dhammaan gaari karaan in ka badan 90%.
Farsamada bandhigga wejiga
Soo saarista unugyada difaaca jirka ee monoclonal ayaa markii hore la diyaariyey iyadoo la isticmaalayo hybridoma monoclonal antibody technology si loo soo saaro mouse hybridoma monoclonal antibody. Gaar ahaan, iyada oo la isku darayo unugyada beeryarada ee jiirarka la tallaalay ee leh unugyada myeloma ee bini'aadamka ama jiirarka, unugyada hybridoma ayaa la sameeyay, kuwaas oo qarsoodi ah hybridoma monoclonal antibody antibody. Beddelka bini'aadantinimada ee unugyada difaaca jirka ee monoclonal mouse iyo wax ka beddelka injineernimada hidaha ee unugyada difaaca si loo siiyo gobolka immunoglobulin joogto ah, difaacooda waa la yarayn karaa. Monoclonal antibody injineernimada ayaa si weyn loogu isticmaalaa codsiyada biomedical iyo kiliinikada.
Alpha Lifetech waxay bixin kartaa adeegyada injineernimada hal-joojin ka-hortagga jidhka laga bilaabo naqshadaynta borotokoolka cilmi-baarista saxda ah ilaa helitaanka isku-xidhka daroogada-diidka (ADCs) iyo xaqiijinta xayawaanka. Isku-xidhayaasha daroogada ka-hortagga ah (ADCs) waxay dawooyinka kiimoterabiga gaadhsiin karaan unugyada kansarka. Ka dib markii lagu xidho bartilmaameedyo gaar ah oo lagu muujiyay unugyada kansarka, ADC waxay ku sii daysaa daawooyinka cytotoxic unugyada kansarka. Alpha Lifetech waxay sidoo kale siin kartaa macaamiisha adeegyo korriin isku xidhidhnimo oo dhammaystiran. Hagaajinta isbeddelka horumarsan iyo tignoolajiyada baadhista phage-ga sare-u-qaadista, unugyada difaaca jirka ee xidhiidh gaar ah leh ayaa marka hore la baaraa, ka dibna isbeddellada amino acid ayaa la soo bandhigay si loo abuuro noocyo kala duwan. Ka dib, unugyada difaaca jirka ee sarreeya ayaa la qiimeeyay oo la baadhay iyadoo la adeegsanayo farsamooyinka baaritaanka. Ka dib wareegyo badan oo wanaajinta iyo falanqaynta qaabdhismeedka, unugyada difaaca jirka oo leh xiriir sare iyo gaar gaar ah ayaa ugu dambeyntii la helay.
Alpha Lifetech waxay dhisi kartaa noocyo kala duwan oo ka mid ah maktabadaha soo bandhiga antibody phage, oo ay ku jiraan maktabadaha difaaca jirka, maktabadaha asalka ah, maktabadaha synthetic semi, iyo maktabadaha synthetic. Iyada oo ku saleysan awoodda sare ee maktabadaha antibody-ga, unugyada difaaca jirka ee aadka u gaarka ah ayaa la heli karaa. Qaybaha phagemid ee badan sida pMECS, pComb3X, iyo pCANTAB 5E ayaa la bixin karaa, iyo sidoo kale noocyada sida TG1 E. coli, XL1 Blue, iyo ER2738. Marka lagu daro awoodda maktabadda oo ah ilaa 10^9, heerka gelinta jajabka bartilmaameedka ee maktabadda ayaa sidoo kale aad u sarreeya, abuurista shuruudo ku filan oo lagu baaro unugyada difaaca ee qanciya macaamiisha. Jaantuska socodka ee adeega diyaarinta kahortaga jidhka ee Alpha Lifetech ee ku salaysan tignoolajiyada soo bandhigida fage waxa lagu muujiyay sawirka 1.
Jaantuska.1 Habka jaantuska loogu talagalay diyaarinta unugyada difaaca jirka ee monoclonal ee ku salaysan tignoolajiyada bandhigga phage.
Bandhiga Wajiga Socodka Shaqo Soo Saarka Antibody
| Talaabooyinka | Nuxurka Adeegga | Jadwalka waqtiga |
|---|---|---|
| Tallaabada 1: Tallaalka xoolaha | (1) Tallaalka xoolaha 4 jeer, tallaalka xoojinta 1 dose, wadar ahaan 5 qiyaasood ayaa la tallaalay. (2) Serum taban ka hor inta aan la ururin tallaalka, iyo ELISA waxaa lagu sameeyay qiyaasta afaraad si loo ogaado titer serum. (3) Haddii serum antibody titer ee qiyaasta afraad ay buuxiso shuruudaha, hal qiyaas oo dheeraad ah oo tallaal ah ayaa la qaadan doonaa 7 maalmood ka hor ururinta dhiigga. Haddii ay buuxin waydo shuruudaha, tallaalka joogtada ahi wuu sii socon doonaa. (4) Awood u qalma, ururinta dhiigga iyo kala-soocidda monocytes | 10 todobaad |
| Tallaabada 2: Diyaarinta cDNA | (1) PBMC Wadarta Soo saarista RNA (Qalabka Soosaarida RNA) (2) Daacadnimada sare ee RT-PCR diyaarinta cDNA (xirmada qoraalka dib u noqoshada) | 1 maalin |
| Tallaabada 3: Dhismaha Maktabadda Kahortagga Jirka | (1) Isticmaalka cDNA qaab ahaan, hiddo-wadaha waxaa lagu xoojiyay laba wareeg oo PCR ah. (2) Dhismaha wejiga iyo isbeddelka: qaybinta hidda-wadaha phagemid vector, isbeddelka korantada ee bakteeriyada martida loo yahay TG1, dhismaha maktabadda antibody. (3) Aqoonsiga: Si aan kala sooc lahayn u dooro 24 clone, PCR-ga aqoonsiga heerka togan+heerka gelinta. (4) Diyaarinta phage-ga la caawiyay: M13 phage amplification+nadiifinta. (5) Samatabbixinta maktabadda wejiga | 3-4 toddobaad |
| Talaabada 4: Baadhitaanka Maktabada ee Antibody (3 wareeg) | (1) Baadhitaanka 3-wareega ah ee ugu talagalka ah (baaritaanka wejiga adag): baarista cadaadiska si meesha looga saaro unugyada difaaca jirka ee aan gaarka ahayn ilaa iyo inta ugu badan ee suurtogalka ah. (2) bacteriophage-ka-weyn ee hal-bowlaha ah oo la xushay+IPTG odhaah ay keentay+ELISA ogaanshaha clones togan. (3) Dhammaan clones togan ayaa loo xushay isku xigxiga hiddo-wadaha. | 4-5 toddobaad |
Adeegyada Taageerada
Waxaan bixin karnaa adeegyada dhismaha maktabadda difaaca jirka ee xayawaanka ku saleysan iyo adeegyada baarista dabiiciga ah ee ka hortagga jirka iyadoo loo eegayo baahida macaamiisha
Bartilmaameedyo Badan
Waxaa jira adeegyo badan oo bartilmaameed ka hortag ah: borotiinada, peptides, molecules yar yar, fayrasyada, borotiinada xuubka, mRNA, iwm.
Vectors badan
Adeegga dhismaha maktabadda ee shakhsi ahaaneed, waxaanu bixin karnaa noocyo kala duwan oo bakteeriyayaal ah oo ay ku jiraan PMECS, pComb3X, iyo pCANTAB 5E, oo aan wax ka beddelno iyadoo loo eegayo baahiyaha macaamiisha
Madal Platform
Awoodda kaydinta waxay gaari kartaa 10 ^ 8-10 ^ 9, heerarka gelinta ayaa dhamaantood ka sarreeya 90%, iyo xiriirka unugyada difaaca jirka ee lagu helo baaritaanka guud ahaan waa heerka nM pM
Adeegga Horumarinta Kahortagga Jirka ee Monoclonal
Waxaan bixin karnaa adeegyo horumarineed oo tayo sare leh, nadiif ah oo aad u gaar ah, oo ay ku jiraan soo saarida unugyada difaaca jirka ee mouse monoclonal iyo unugyada difaaca jirka ee bakaylaha monoclonal
Platform Technology Hybridoma
Oo ay ku jiraan barnaamijka tallaalka, adeegyada diyaarinta ka-hortagga jidhka, nadiifinta ka-hortagga unugga, kala-soocidda sare-u-qaadista antibody-ga, ansaxinta antibody-ga, iwm
Hal B Platform Kala Soocida Unugyada
Alpha Lifetech waxay faa'iidooyin u leedahay waqtiga baaritaanka iyo helitaanka unugyada difaaca jirka oo tayo sare leh. Waxay bixin kartaa naqshadaynta antigen-ka, isku-dhafka, iyo wax-ka-beddelka, difaaca xayawaanka, baadhista kobcinta unugyada B ee hal unug, isku xigxiga hal unug.
Bandhiga Wajiga Horumarinta Kahortagga Jirka
Alpha Lifetech waxay bixin kartaa bandhiga bandhiga antibody-ka adeegyada farsamo ee diyaarinta antibody, nadiifinta antibody, isku xigxiga antibody, iwm.
Haddii aad wax su'aalo ah qabtid, fadlan xor u noqo inaad nala soo xiriirto wakhti kasta.
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2018-07-16 
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