Hordhac Maktabadaha Antibody-ga ee la isku-dhafan yahay

Maktabadda antibody-ga ee la isku daray, oo sidoo kale loo yaqaan maktabadda De novo, waa hab macmal ah oo adeegsada farsamooyinka sida isku-darka DNA-da ama bandhigga fage-ka si loo naqshadeeyo loona sameeyo gobollada kala duwan ee antibody-ga oo dhammaystiran, oo ay ku jiraan gobollada qaab-dhismeedka iyo CDR-yada, iyada oo aan lagu tiirsanayn maktabadaha antibody-ga ee aan caadiga ahayn.

Maktabadda antibody-ga ee nus-synthetic-ka ah waxaa la sameeyaa iyadoo la isku darayo maktabadaha antibody-ga ee dabiiciga ah iyo kala duwanaanshaha synthetic-ga ah. Badanaa waxay ku lug leedahay isku-darka oligonucleotides-ka DNA-da si loo soo saaro qaybo kala duwan oo go'aaminaya isku-dhafan (CDRs), kuwaas oo markaa lagu daro qaab-dhismeedka antibody-ga ee joogtada ah ee laga helo ilo dabiici ah sida unugyada B ee aadanaha ama xayawaanka. CDR-ka la sameeyay wuxuu ku soo bandhigayaa kala duwanaansho maktabadda, taasoo keentay in antibody-yadu ay bartilmaameedsadaan noocyo badan oo epitopes ah. Maktabadda antibody-ga ee nus-synthetic-ka ah waxay bixisaa tanaasul u dhexeeya kala duwanaanshaha dabiiciga ah ee nidaamka difaaca jirka iyo kala duwanaanshaha la xakameeyey ee lagu gaaro hababka synthetic-ga.

Kala duwanaanshaha u dhexeeya maktabadaha unugyada difaaca jirka ee aan caadiga ahayn iyo kuwa aan caadiga ahayn waxay ku xiran tahay isha hiddo-sidayaasha unugyada difaaca jirka ee aan caadiga ahayn. Dhanka kale, farqiga u dhexeeya maktabadda unugyada difaaca jirka ee nus-sameysan iyo kuwa la sameeyay waxay ku jirtaa in tan hore ay ka kooban tahay qayb ka mid ah qaybaha unugyada difaaca jirka ee aan caadiga ahayn, sida silsiladda fudud ama silsiladda culus, oo mid ka mid ah oo keliya lagu sameeyay in vitro; halka tan la sameeyay ay gebi ahaanba ka soo baxday isku-darka macmal ah oo ay samaysay PCR in vitro.

Alpha Lifetech waxay bixin kartaa

Shirkadda Alpha Lifetech Inc.waxay bixin kartaa maktabad antibody ah oo nus-samaysan/la sameeyay oo ka timid xayawaanka iyo aadanaha iyadoo lagu saleynayo barnaamijkeena xirfadeed ee daahfurka antibody-ga. Iyada oo leh sannado khibrad ah oo ku saabsan wax ka beddelka hidde-sidaha iyo dhismaha maktabadda, maktabadaha la sameeyay ayaa la dhisi karaa kuwaas oo awood u leh inay soo saaraan antibody-yada macmalka ah oo leh isku xirnaan iyo gaar ah oo ka baxsan awoodda antibody-yada dabiiciga ah.Alpha Lifetech'sKhubaradu waxay ku faanayaan inay ku dhawaaqaan inaan dammaanad qaadi karno heer sare oo guul ah oo ku saabsan dhismaha maktabad antibody ah oo ka kooban 10^8 - 10^10 clones madax-bannaan.

Shirkadda Alpha Lifetech Inc.waxay ku faantaa inay bixiso adeegyo dhisme oo dhammaystiran oo maktabadda antibody-ga ah si loo daboolo baahiyaha kala duwan ee cilmi-baarayaasha caalamiga ah, oo ay ku jiraan scFv, Fab, VHH antibody, iyo maktabado gaar ah. Hadafkayagu waa inaan fahanno oo aan daboolno baahiyaha macaamiisha kala duwan iyo inaan ka caawinno dhibaatooyinka soo socda iyo kuwa soo baxaya ee shaqada cilmi-baarista.

Habka Adeegga Dhismaha Maktabadaha Antibody-ga ee Isku-dhafan

Naqshadeynta
Marxaladda naqshadaynta waxay ku lug leedahay xulashada qaab-dhismeedka antibody-ga iyo gobollada go'aaminta dhammaystirka (CDRs) iyadoo lagu saleynayo taxanaha antibody-ga ee jira ama xogta qaab-dhismeedka. Antibodies-ka CDR ee synthetic-ga ayaa sidoo kale loo qaabeyn karaa inay soo bandhigaan shaqooyin gaar ah ama ay kor u qaadaan sifooyinka isku xidhka.

Isku-darka
DNA-da synthetic-ga ah ee codaynaysa taxanaha antibody-ga ee loogu talagalay, oo ay ku jiraan labada gobol ee qaab-dhismeedka iyo CDR-yada, waxaa lagu sameeyaa iyadoo la adeegsanayo hababka kiimikada ama ensaymikada.

Shirka
Qaybaha DNA-da ee la sameeyay waxaa lagu soo ururiyaa vectors-ka muujinta antibody-ga iyadoo la adeegsanayo farsamooyin sida PCR, ligation, ama Gibson assembly. Vectors-kan waxaa markaa lagu soo bandhigi karaa nidaamyada muujinta sida bakteeriyada, khamiirka, ama unugyada naasleyda si loo soo saaro antibody-ga.

Baaritaanka iyo Xulashada
Maktabadaha difaaca jirka ee la dhisay waxaa lagu baaraa oo loo xushaa unugyada difaaca jirka ee leh sifooyinka la rabo iyadoo la adeegsanayo hababka baaritaanka ee wax soo saarka sare leh. Tani waxay ku lug yeelan kartaa farsamooyin sida soo bandhigida phage, soo bandhigida khamiirka, ama soo bandhigida ribosome, iyadoo ku xiran qaabka maktabadda iyo codsiga.

Adeegga Dhismaha Maktabadaha Ka-hortagga Jirka ee Isku-dhafan

Shirkadda Alpha Lifetech Inc.Waxay macaamiisha siisaa naqshad hal-joojin ah iyo adeeg isku-dhafan oo maktabadaha antibody-ga ah iyadoo la isku xidho silsiladda culus iyo kuwa fudud ee hiddo-wadaha V-ga vector-ka phage-ka iyadoo la adeegsanayo nidaamka isku-darka goobta gaarka ah ee Cre-lox si loo abuuro repertoire Fabs ah oo lagu soo bandhigay phage oo ka kooban 6.5 × 10^10 clones. Maktabaddu waxay soo saartay Abs oo ka dhan ah antigens badan, qaarkoodna waxay leeyihiin isku-xirnaan nanomolar ah. Soo saarista maktabadda antibody-ga aadanaha waxay ku salaysan tahay tignoolajiyada bandhigga phage-ka M13 (oo lagu muujiyay sawirka hoose).